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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli.1 PublicationBy similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271PROSITE-ProRule annotationBy similarity
Metal bindingi328 – 3281CalciumBy similarity
Metal bindingi357 – 3571CalciumBy similarity
Metal bindingi359 – 3591CalciumBy similarity
Active sitei374 – 3741PROSITE-ProRule annotationBy similarity
Metal bindingi408 – 4081CalciumBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. G-protein beta/gamma-subunit complex binding Source: RGD
  3. phosphatidylinositol phospholipase C activity Source: UniProtKB
  4. phospholipase C activity Source: RGD
  5. phospholipid binding Source: RGD
  6. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. phospholipid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2By similarity
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621004. Plcb2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
  2. Golgi apparatus Source: RGD
  3. G-protein beta/gamma-subunit complex Source: RGD
  4. plasma membrane Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118311831-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2PRO_0000088490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei952 – 9521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO89040.
PRIDEiO89040.

PTM databases

PhosphoSiteiO89040.

Expressioni

Tissue specificityi

Expressed in the gustatory cells of the circumvallate papillae. Not detected in non-sensory lingual epithelium, brain, parotid gland, liver, uterus, lung, heart, adrenal gland, ovary, spleen, pancreas, intestine, thymus, kidney or muscle.2 Publications

Gene expression databases

GenevestigatoriO89040.

Interactioni

Subunit structurei

Interacts with RAC1.By similarity

Protein-protein interaction databases

BioGridi250044. 2 interactions.
STRINGi10116.ENSRNOP00000011911.

Structurei

3D structure databases

ProteinModelPortaliO89040.
SMRiO89040. Positions 11-807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini312 – 463152PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini550 – 666117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini673 – 77199C2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1077 – 114670Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi492 – 54958Glu-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiO89040.
KOiK05858.
PhylomeDBiO89040.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms may be produced.1 Publication

Isoform 1 (identifier: O89040-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLNPVLLP PKVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT
60 70 80 90 100
HQSKEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTFT
110 120 130 140 150
VVSGPDMVGL TFHNFVSYKE NVGKDWAEDV LALAKHPMTA NASRSTFLDK
160 170 180 190 200
ILVKLKMQLS PEGKIPVKNF FQMFPADRKR VEAALSACHL AKGKNDAINP
210 220 230 240 250
EDFPESVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH LTKFINQKQR
260 270 280 290 300
DPRLNSLLFP PARPEQVQAL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN
310 320 330 340 350
SVLAHDTLRI HQDMTQPLNH YFINSSHNTY LTAGQFSGPS SAEMYRQVLL
360 370 380 390 400
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAVEA IAESAFKTSP
410 420 430 440 450
YPVILSFENH VDSPRQQAKM AEYCRTMFGE TLLTEPLENF PLKPGMPLPS
460 470 480 490 500
PEDLRGKILI KNKKNQFSGP ASPNKKPDGV SEGGFPSSVP VEEDTGWTAE
510 520 530 540 550
DRTEVEEGEE EEEVEEEEEE ESGNLDEEEI KKMQSDEGTA GLEVTAYEEM
560 570 580 590 600
SSLVNYIQPT KFISFEFSAQ KNRSYLVSSF TELKAYELLS KASMQFVDYN
610 620 630 640 650
KRQMSRVYPK GTRMDSSNYM PQMFWNAGCQ MVALNFQTMD LPMQQNMALF
660 670 680 690 700
EFNGQSGYLL KHEFMRRQDK QFNPFSVDRI DVVVATTLSI TVISGQFLSE
710 720 730 740 750
RSVRTYVEVE LFGLPGDPKR RYRTKLSPTA NSINPVWKEE PFIFEKILVP
760 770 780 790 800
ELASLRIAVM EEGGKFIGHR IIPINALHSG YHHLCLRSES NMPLTMPALF
810 820 830 840 850
VFLEMKDYVP DTWADLTVAL ANPIKYFSAH DKKSVKLKEV TGSLPEKLFS
860 870 880 890 900
GIPVASQSNG APVSAGNGST APGTKAKEEA TKEVAEPQTT SLEELRELKG
910 920 930 940 950
VVKLQRRHEK ELRELERRGA RRWEELLQRG AAQLAELQDP AASCKLRPGK
960 970 980 990 1000
GSRKKRIVPC EETIVVPREV LEGPDPRVQD LKDRLEQELQ QQGEEQYRSV
1010 1020 1030 1040 1050
LKRKEQHVTE QIAKMMELAR EKQAAELKSF KETSETDTKE MKKKLEAKRL
1060 1070 1080 1090 1100
ERIQAMTKVT TDKVAQERLK REINNSHIQE VVQAVKQMTE TLERHQEKLE
1110 1120 1130 1140 1150
EKQTACLEQI QAMEKQFQEK ALAEYEAKMK GLEAEVKESM RACFKACFPT
1160 1170 1180
EAEEKPERPC EASEESCPQE PLVNKTDTQE SRL
Length:1,183
Mass (Da):134,883
Last modified:November 1, 1998 - v1
Checksum:iBA3AD63FE074A18C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011035 mRNA. Translation: CAA09465.1.
RefSeqiNP_445930.1. NM_053478.1. [O89040-1]
UniGeneiRn.30033.

Genome annotation databases

GeneIDi85240.
KEGGirno:85240.
UCSCiRGD:621004. rat. [O89040-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011035 mRNA. Translation: CAA09465.1.
RefSeqiNP_445930.1. NM_053478.1. [O89040-1]
UniGeneiRn.30033.

3D structure databases

ProteinModelPortaliO89040.
SMRiO89040. Positions 11-807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250044. 2 interactions.
STRINGi10116.ENSRNOP00000011911.

PTM databases

PhosphoSiteiO89040.

Proteomic databases

PaxDbiO89040.
PRIDEiO89040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85240.
KEGGirno:85240.
UCSCiRGD:621004. rat. [O89040-1]

Organism-specific databases

CTDi5330.
RGDi621004. Plcb2.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiO89040.
KOiK05858.
PhylomeDBiO89040.

Miscellaneous databases

NextBioi617292.
PROiO89040.

Gene expression databases

GenevestigatoriO89040.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of a phospholipase C beta subtype in rat taste cells."
    Roessler P., Kroner C., Freitag J., Noe J., Breer H.
    Eur. J. Cell Biol. 77:253-261(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Circumvallate papilla.
  2. "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
    Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
    J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCB2_RAT
AccessioniPrimary (citable) accession number: O89040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: November 1, 1998
Last modified: March 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.