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O89040 (PLCB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name=PLC-beta-2
Gene names
Name:Plcb2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1183 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli. Ref.1 UniProtKB Q00722

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB Q00722

Cofactor

Binds 1 calcium ion per subunit By similarity. UniProtKB Q00722

Subunit structure

Interacts with RAC1 By similarity. UniProtKB Q00722

Tissue specificity

Expressed in the gustatory cells of the circumvallate papillae. Not detected in non-sensory lingual epithelium, brain, parotid gland, liver, uterus, lung, heart, adrenal gland, ovary, spleen, pancreas, intestine, thymus, kidney or muscle. Ref.1 Ref.2

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16. UniProtKB Q00722

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms may be produced.
Isoform 1 Ref.1 (identifier: O89040-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 118311831-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
PRO_0000088490

Regions

Domain312 – 463152PI-PLC X-box
Domain550 – 666117PI-PLC Y-box
Domain673 – 77199C2
Coiled coil1077 – 114670 Potential
Compositional bias492 – 54958Glu-rich

Sites

Active site3271 By similarity UniProtKB P10688
Active site3741 By similarity UniProtKB P10688
Metal binding3281Calcium By similarity UniProtKB Q00722
Metal binding3571Calcium By similarity UniProtKB Q00722
Metal binding3591Calcium By similarity UniProtKB Q00722
Metal binding4081Calcium By similarity UniProtKB Q00722

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: BA3AD63FE074A18C

FASTA1,183134,883
        10         20         30         40         50         60 
MSLLNPVLLP PKVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT HQSKEMEFLD 

        70         80         90        100        110        120 
VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTFT VVSGPDMVGL TFHNFVSYKE 

       130        140        150        160        170        180 
NVGKDWAEDV LALAKHPMTA NASRSTFLDK ILVKLKMQLS PEGKIPVKNF FQMFPADRKR 

       190        200        210        220        230        240 
VEAALSACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH 

       250        260        270        280        290        300 
LTKFINQKQR DPRLNSLLFP PARPEQVQAL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 

       310        320        330        340        350        360 
SVLAHDTLRI HQDMTQPLNH YFINSSHNTY LTAGQFSGPS SAEMYRQVLL SGCRCVELDC 

       370        380        390        400        410        420 
WKGKPPDEEP IITHGFTMTT DILFKEAVEA IAESAFKTSP YPVILSFENH VDSPRQQAKM 

       430        440        450        460        470        480 
AEYCRTMFGE TLLTEPLENF PLKPGMPLPS PEDLRGKILI KNKKNQFSGP ASPNKKPDGV 

       490        500        510        520        530        540 
SEGGFPSSVP VEEDTGWTAE DRTEVEEGEE EEEVEEEEEE ESGNLDEEEI KKMQSDEGTA 

       550        560        570        580        590        600 
GLEVTAYEEM SSLVNYIQPT KFISFEFSAQ KNRSYLVSSF TELKAYELLS KASMQFVDYN 

       610        620        630        640        650        660 
KRQMSRVYPK GTRMDSSNYM PQMFWNAGCQ MVALNFQTMD LPMQQNMALF EFNGQSGYLL 

       670        680        690        700        710        720 
KHEFMRRQDK QFNPFSVDRI DVVVATTLSI TVISGQFLSE RSVRTYVEVE LFGLPGDPKR 

       730        740        750        760        770        780 
RYRTKLSPTA NSINPVWKEE PFIFEKILVP ELASLRIAVM EEGGKFIGHR IIPINALHSG 

       790        800        810        820        830        840 
YHHLCLRSES NMPLTMPALF VFLEMKDYVP DTWADLTVAL ANPIKYFSAH DKKSVKLKEV 

       850        860        870        880        890        900 
TGSLPEKLFS GIPVASQSNG APVSAGNGST APGTKAKEEA TKEVAEPQTT SLEELRELKG 

       910        920        930        940        950        960 
VVKLQRRHEK ELRELERRGA RRWEELLQRG AAQLAELQDP AASCKLRPGK GSRKKRIVPC 

       970        980        990       1000       1010       1020 
EETIVVPREV LEGPDPRVQD LKDRLEQELQ QQGEEQYRSV LKRKEQHVTE QIAKMMELAR 

      1030       1040       1050       1060       1070       1080 
EKQAAELKSF KETSETDTKE MKKKLEAKRL ERIQAMTKVT TDKVAQERLK REINNSHIQE 

      1090       1100       1110       1120       1130       1140 
VVQAVKQMTE TLERHQEKLE EKQTACLEQI QAMEKQFQEK ALAEYEAKMK GLEAEVKESM 

      1150       1160       1170       1180 
RACFKACFPT EAEEKPERPC EASEESCPQE PLVNKTDTQE SRL 

« Hide

References

[1]"Identification of a phospholipase C beta subtype in rat taste cells."
Roessler P., Kroner C., Freitag J., Noe J., Breer H.
Eur. J. Cell Biol. 77:253-261(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Circumvallate papilla.
[2]"Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ011035 mRNA. Translation: CAA09465.1.
RefSeqNP_445930.1. NM_053478.1. [O89040-1]
UniGeneRn.30033.

3D structure databases

ProteinModelPortalO89040.
SMRO89040. Positions 11-807.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250044. 1 interaction.
STRING10116.ENSRNOP00000011911.

PTM databases

PhosphoSiteO89040.

Proteomic databases

PaxDbO89040.
PRIDEO89040.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID85240.
KEGGrno:85240.
UCSCRGD:621004. rat. [O89040-1]

Organism-specific databases

CTD5330.
RGD621004. Plcb2.

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000232046.
HOVERGENHBG053609.
KOK05858.
PhylomeDBO89040.

Gene expression databases

GenevestigatorO89040.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFPIRSF000956. PLC-beta. 1 hit.
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617292.
PROO89040.

Entry information

Entry namePLCB2_RAT
AccessionPrimary (citable) accession number: O89040
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families