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O89040

- PLCB2_RAT

UniProt

O89040 - PLCB2_RAT

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli.By similarity1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei327 – 3271By similarityPROSITE-ProRule annotation
    Metal bindingi328 – 3281CalciumBy similarity
    Metal bindingi357 – 3571CalciumBy similarity
    Metal bindingi359 – 3591CalciumBy similarity
    Active sitei374 – 3741By similarityPROSITE-ProRule annotation
    Metal bindingi408 – 4081CalciumBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. G-protein beta/gamma-subunit complex binding Source: RGD
    3. phosphatidylinositol phospholipase C activity Source: UniProtKB
    4. phospholipase C activity Source: RGD
    5. phospholipid binding Source: RGD
    6. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProtKB
    2. intracellular signal transduction Source: UniProtKB
    3. phospholipid catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-beta-2
    Phospholipase C-beta-2
    Short name:
    PLC-beta-2
    Gene namesi
    Name:Plcb2By similarity
    OrganismiRattus norvegicus (Rat)Imported
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621004. Plcb2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. Golgi apparatus Source: RGD
    3. G-protein beta/gamma-subunit complex Source: RGD
    4. plasma membrane Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 118311831-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2PRO_0000088490Add
    BLAST

    Proteomic databases

    PaxDbiO89040.
    PRIDEiO89040.

    PTM databases

    PhosphoSiteiO89040.

    Expressioni

    Tissue specificityi

    Expressed in the gustatory cells of the circumvallate papillae. Not detected in non-sensory lingual epithelium, brain, parotid gland, liver, uterus, lung, heart, adrenal gland, ovary, spleen, pancreas, intestine, thymus, kidney or muscle.2 Publications

    Gene expression databases

    GenevestigatoriO89040.

    Interactioni

    Subunit structurei

    Interacts with RAC1.By similarity

    Protein-protein interaction databases

    BioGridi250044. 1 interaction.
    STRINGi10116.ENSRNOP00000011911.

    Structurei

    3D structure databases

    ProteinModelPortaliO89040.
    SMRiO89040. Positions 11-807.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini312 – 463152PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini550 – 666117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini673 – 77199C2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1077 – 114670Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi492 – 54958Glu-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000232046.
    HOVERGENiHBG053609.
    KOiK05858.
    PhylomeDBiO89040.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028403. PLC-beta2.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF10. PTHR10336:SF10. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms may be produced.1 Publication

    Isoform 1 (identifier: O89040-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLLNPVLLP PKVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT     50
    HQSKEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTFT 100
    VVSGPDMVGL TFHNFVSYKE NVGKDWAEDV LALAKHPMTA NASRSTFLDK 150
    ILVKLKMQLS PEGKIPVKNF FQMFPADRKR VEAALSACHL AKGKNDAINP 200
    EDFPESVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH LTKFINQKQR 250
    DPRLNSLLFP PARPEQVQAL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 300
    SVLAHDTLRI HQDMTQPLNH YFINSSHNTY LTAGQFSGPS SAEMYRQVLL 350
    SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAVEA IAESAFKTSP 400
    YPVILSFENH VDSPRQQAKM AEYCRTMFGE TLLTEPLENF PLKPGMPLPS 450
    PEDLRGKILI KNKKNQFSGP ASPNKKPDGV SEGGFPSSVP VEEDTGWTAE 500
    DRTEVEEGEE EEEVEEEEEE ESGNLDEEEI KKMQSDEGTA GLEVTAYEEM 550
    SSLVNYIQPT KFISFEFSAQ KNRSYLVSSF TELKAYELLS KASMQFVDYN 600
    KRQMSRVYPK GTRMDSSNYM PQMFWNAGCQ MVALNFQTMD LPMQQNMALF 650
    EFNGQSGYLL KHEFMRRQDK QFNPFSVDRI DVVVATTLSI TVISGQFLSE 700
    RSVRTYVEVE LFGLPGDPKR RYRTKLSPTA NSINPVWKEE PFIFEKILVP 750
    ELASLRIAVM EEGGKFIGHR IIPINALHSG YHHLCLRSES NMPLTMPALF 800
    VFLEMKDYVP DTWADLTVAL ANPIKYFSAH DKKSVKLKEV TGSLPEKLFS 850
    GIPVASQSNG APVSAGNGST APGTKAKEEA TKEVAEPQTT SLEELRELKG 900
    VVKLQRRHEK ELRELERRGA RRWEELLQRG AAQLAELQDP AASCKLRPGK 950
    GSRKKRIVPC EETIVVPREV LEGPDPRVQD LKDRLEQELQ QQGEEQYRSV 1000
    LKRKEQHVTE QIAKMMELAR EKQAAELKSF KETSETDTKE MKKKLEAKRL 1050
    ERIQAMTKVT TDKVAQERLK REINNSHIQE VVQAVKQMTE TLERHQEKLE 1100
    EKQTACLEQI QAMEKQFQEK ALAEYEAKMK GLEAEVKESM RACFKACFPT 1150
    EAEEKPERPC EASEESCPQE PLVNKTDTQE SRL 1183
    Length:1,183
    Mass (Da):134,883
    Last modified:November 1, 1998 - v1
    Checksum:iBA3AD63FE074A18C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011035 mRNA. Translation: CAA09465.1.
    RefSeqiNP_445930.1. NM_053478.1. [O89040-1]
    UniGeneiRn.30033.

    Genome annotation databases

    GeneIDi85240.
    KEGGirno:85240.
    UCSCiRGD:621004. rat. [O89040-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011035 mRNA. Translation: CAA09465.1 .
    RefSeqi NP_445930.1. NM_053478.1. [O89040-1 ]
    UniGenei Rn.30033.

    3D structure databases

    ProteinModelPortali O89040.
    SMRi O89040. Positions 11-807.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250044. 1 interaction.
    STRINGi 10116.ENSRNOP00000011911.

    PTM databases

    PhosphoSitei O89040.

    Proteomic databases

    PaxDbi O89040.
    PRIDEi O89040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 85240.
    KEGGi rno:85240.
    UCSCi RGD:621004. rat. [O89040-1 ]

    Organism-specific databases

    CTDi 5330.
    RGDi 621004. Plcb2.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000232046.
    HOVERGENi HBG053609.
    KOi K05858.
    PhylomeDBi O89040.

    Miscellaneous databases

    NextBioi 617292.
    PROi O89040.

    Gene expression databases

    Genevestigatori O89040.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028403. PLC-beta2.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF10. PTHR10336:SF10. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a phospholipase C beta subtype in rat taste cells."
      Roessler P., Kroner C., Freitag J., Noe J., Breer H.
      Eur. J. Cell Biol. 77:253-261(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Circumvallate papilla.
    2. "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
      Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
      J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCB2_RAT
    AccessioniPrimary (citable) accession number: O89040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3