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O89040

- PLCB2_RAT

UniProt

O89040 - PLCB2_RAT

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Protein
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Gene
Plcb2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli.By similarity1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Binds 1 calcium ion per subunit By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271 By similarityBy similarity
Metal bindingi328 – 3281Calcium By similarityBy similarity
Metal bindingi357 – 3571Calcium By similarityBy similarity
Metal bindingi359 – 3591Calcium By similarityBy similarity
Active sitei374 – 3741 By similarityBy similarity
Metal bindingi408 – 4081Calcium By similarityBy similarity

GO - Molecular functioni

  1. G-protein beta/gamma-subunit complex binding Source: RGD
  2. calcium ion binding Source: InterPro
  3. phosphatidylinositol phospholipase C activity Source: UniProtKB
  4. phospholipase C activity Source: RGD
  5. phospholipid binding Source: RGD
  6. signal transducer activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. phospholipid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621004. Plcb2.

Subcellular locationi

GO - Cellular componenti

  1. G-protein beta/gamma-subunit complex Source: RGD
  2. Golgi apparatus Source: RGD
  3. cytosol Source: RGD
  4. plasma membrane Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118311831-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
PRO_0000088490Add
BLAST

Proteomic databases

PaxDbiO89040.
PRIDEiO89040.

PTM databases

PhosphoSiteiO89040.

Expressioni

Tissue specificityi

Expressed in the gustatory cells of the circumvallate papillae. Not detected in non-sensory lingual epithelium, brain, parotid gland, liver, uterus, lung, heart, adrenal gland, ovary, spleen, pancreas, intestine, thymus, kidney or muscle.2 Publications

Gene expression databases

GenevestigatoriO89040.

Interactioni

Subunit structurei

Interacts with RAC1 By similarity.By similarity

Protein-protein interaction databases

BioGridi250044. 1 interaction.
STRINGi10116.ENSRNOP00000011911.

Structurei

3D structure databases

ProteinModelPortaliO89040.
SMRiO89040. Positions 11-807.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini312 – 463152PI-PLC X-box
Add
BLAST
Domaini550 – 666117PI-PLC Y-box
Add
BLAST
Domaini673 – 77199C2
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1077 – 114670 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi492 – 54958Glu-rich
Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
KOiK05858.
PhylomeDBiO89040.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms may be produced.

Isoform 11 Publication (identifier: O89040-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLLNPVLLP PKVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT     50
HQSKEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTFT 100
VVSGPDMVGL TFHNFVSYKE NVGKDWAEDV LALAKHPMTA NASRSTFLDK 150
ILVKLKMQLS PEGKIPVKNF FQMFPADRKR VEAALSACHL AKGKNDAINP 200
EDFPESVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH LTKFINQKQR 250
DPRLNSLLFP PARPEQVQAL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN 300
SVLAHDTLRI HQDMTQPLNH YFINSSHNTY LTAGQFSGPS SAEMYRQVLL 350
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAVEA IAESAFKTSP 400
YPVILSFENH VDSPRQQAKM AEYCRTMFGE TLLTEPLENF PLKPGMPLPS 450
PEDLRGKILI KNKKNQFSGP ASPNKKPDGV SEGGFPSSVP VEEDTGWTAE 500
DRTEVEEGEE EEEVEEEEEE ESGNLDEEEI KKMQSDEGTA GLEVTAYEEM 550
SSLVNYIQPT KFISFEFSAQ KNRSYLVSSF TELKAYELLS KASMQFVDYN 600
KRQMSRVYPK GTRMDSSNYM PQMFWNAGCQ MVALNFQTMD LPMQQNMALF 650
EFNGQSGYLL KHEFMRRQDK QFNPFSVDRI DVVVATTLSI TVISGQFLSE 700
RSVRTYVEVE LFGLPGDPKR RYRTKLSPTA NSINPVWKEE PFIFEKILVP 750
ELASLRIAVM EEGGKFIGHR IIPINALHSG YHHLCLRSES NMPLTMPALF 800
VFLEMKDYVP DTWADLTVAL ANPIKYFSAH DKKSVKLKEV TGSLPEKLFS 850
GIPVASQSNG APVSAGNGST APGTKAKEEA TKEVAEPQTT SLEELRELKG 900
VVKLQRRHEK ELRELERRGA RRWEELLQRG AAQLAELQDP AASCKLRPGK 950
GSRKKRIVPC EETIVVPREV LEGPDPRVQD LKDRLEQELQ QQGEEQYRSV 1000
LKRKEQHVTE QIAKMMELAR EKQAAELKSF KETSETDTKE MKKKLEAKRL 1050
ERIQAMTKVT TDKVAQERLK REINNSHIQE VVQAVKQMTE TLERHQEKLE 1100
EKQTACLEQI QAMEKQFQEK ALAEYEAKMK GLEAEVKESM RACFKACFPT 1150
EAEEKPERPC EASEESCPQE PLVNKTDTQE SRL 1183
Length:1,183
Mass (Da):134,883
Last modified:November 1, 1998 - v1
Checksum:iBA3AD63FE074A18C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011035 mRNA. Translation: CAA09465.1.
RefSeqiNP_445930.1. NM_053478.1. [O89040-1]
UniGeneiRn.30033.

Genome annotation databases

GeneIDi85240.
KEGGirno:85240.
UCSCiRGD:621004. rat. [O89040-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011035 mRNA. Translation: CAA09465.1 .
RefSeqi NP_445930.1. NM_053478.1. [O89040-1 ]
UniGenei Rn.30033.

3D structure databases

ProteinModelPortali O89040.
SMRi O89040. Positions 11-807.
ModBasei Search...

Protein-protein interaction databases

BioGridi 250044. 1 interaction.
STRINGi 10116.ENSRNOP00000011911.

PTM databases

PhosphoSitei O89040.

Proteomic databases

PaxDbi O89040.
PRIDEi O89040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 85240.
KEGGi rno:85240.
UCSCi RGD:621004. rat. [O89040-1 ]

Organism-specific databases

CTDi 5330.
RGDi 621004. Plcb2.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000232046.
HOVERGENi HBG053609.
KOi K05858.
PhylomeDBi O89040.

Miscellaneous databases

NextBioi 617292.
PROi O89040.

Gene expression databases

Genevestigatori O89040.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a phospholipase C beta subtype in rat taste cells."
    Roessler P., Kroner C., Freitag J., Noe J., Breer H.
    Eur. J. Cell Biol. 77:253-261(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Circumvallate papilla.
  2. "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
    Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
    J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCB2_RAT
AccessioniPrimary (citable) accession number: O89040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi