C-X-C chemokine receptor type 7 - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

O89039 (CXCR7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
C-X-C chemokine receptor type 7
Short name=CXC-R7
Short name=CXCR-7

Alternative name(s):
Chemokine orphan receptor 1
G-protein coupled receptor RDC1 homolog
Short name=RDC-1

Gene names

Name:	Cxcr7
Synonyms:	Cmkor1, Rdc1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Ref.6
Subunit structure	Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Early endosome By similarity. Recycling endosome By similarity. Note: Localized mainly in perinuclear regions in neurons and in early endosomes in T-lymphocytes and some other cell types, with very low levels detected on the cell surface. May spontaneously cycle between the plasma membrane and endosomal compartments By similarity. Ref.7
Tissue specificity	Expressed in vascular smooth muscle cells (at protein level). In brain, expressed in blood vessels, pyramidal cells in hippocampal subfield CA3, mature dentate gyrus granule cells, ventricle walls, olfactory bulb, accumbens shell, supraoptic, lateroanterior and ventromedial hypothalamic nuclei, medial region of thalamus, and motor nuclei, central gray and raphe magnus nucleus of brain stem. Detected in primary neurons, GABAergic neurons, astrocytes, cerebral cortex, ventral striatum and choroid plexus. Not detected in mesencephalon. Ref.4 Ref.5 Ref.6 Ref.7
Developmental stage	Expressed in the ventral and dorsal parts of telencephalon at all developmental stages of brain analyzed (E14 to P56). Strong expression detected in the cranial connective tissue surrounding the brain at E14-E15. In the cortex, expressed mainly in the marginal zone at preplate stage (E14), with expression increasing strongly in the marginal zone/layer I between E15 and E18 and declining rapidly in layer I after P0. Expression emerges in the lateral part of cortical plate at E15 and increases in the medial and lateral parts between E15 and E17. Expression not detected in the cortical ventricular and subventricular zones at the early embryonic stages but emerges at E18. Expressed in GABAergic precursors and in some reelin-expressing Cajal-Ratzius cells, in neurons forming the cortical plate and sparsely in the developing dentate gyrus and cerebellar external germinal layer. In the ventral telencephalon, expressed in the germinative zone of the ganglionic eminences and in GABAergic neurons forming the caudate putamen between E14 and E18. Ref.4
Induction	By ischemia. Up-regulated in the cingulate, retrosplenial and frontal areas of the cortex ipsilateral to middle cerebral artery occlusion (MCAO) by 163% at 6 hours, 220% at 2 days and 89% at 4 days after ischemia-onset, with expression reduced back to control level at 10 days after MCAO. Expression is almost undetectable in the infarct area between days 2 and 10 after MCAO. Ref.5
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Cytoplasm Endosome Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW chemotaxis Inferred from electronic annotation. Source: InterPro negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara vasculogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	early endosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell recycling endosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	C-X-C chemokine receptor activity Inferred from electronic annotation. Source: Compara coreceptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 362

362

C-X-C chemokine receptor type 7

PRO_0000070103

Regions

Topological domain

1 – 47

Extracellular Potential

Transmembrane

48 – 68

Helical; Name=1; Potential

Topological domain

69 – 81

Cytoplasmic Potential

Transmembrane

82 – 102

Helical; Name=2; Potential

Topological domain

103 – 118

Extracellular Potential

Transmembrane

119 – 139

Helical; Name=3; Potential

Topological domain

140 – 162

Cytoplasmic Potential

Transmembrane

163 – 183

Helical; Name=4; Potential

Topological domain

184 – 213

Extracellular Potential

Transmembrane

214 – 234

Helical; Name=5; Potential

Topological domain

235 – 252

Cytoplasmic Potential

Transmembrane

253 – 273

Helical; Name=6; Potential

Topological domain

274 – 296

Extracellular Potential

Transmembrane

297 – 319

Helical; Name=7; Potential

Topological domain

320 – 362

Cytoplasmic Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

117 ↔ 196

By similarity

Experimental info

Sequence conflict

I → S in CAA09370. Ref.1

Sequence conflict

161

R → L in CAA09370. Ref.1

Sequence conflict

361

T → A in CAA09370. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O89039 [UniParc].

Last modified January 25, 2012. Version 2.
Checksum: C47D1DD678697CFD
Show »

FASTA

362

41,650

        10         20         30         40         50         60 
MDVHLFDYVE PGNYSDINWP CNSSDCIVVD TVQCPAMPNK NVLLYTLSFI YIFIFVIGMI 

        70         80         90        100        110        120 
ANSVVVWVNI QAKTTGYDTH CYILNLAIAD LWVVITIPVW VVSLVQHNQW PMGELTCKIT 

       130        140        150        160        170        180 
HLIFSINLFG SIFFLACMSV DRYLSITYFT STSSYKKKMV RRVVCVLVWL LAFFVSLPDT 

       190        200        210        220        230        240 
YYLKTVTSAS NNETYCRSFY PEHSIKEWLI GMELVSVILG FAVPFTIIAI FYFLLARAMS 

       250        260        270        280        290        300 
ASGDQEKHSS RKIIFSYVVV FLVCWLPYHF VVLLDIFSIL HYIPFTCQLE NVLFTALHVT 

       310        320        330        340        350        360 
QCLSLVHCCV NPVLYSFINR NYRYELMKAF IFKYSAKTGL TKLIDASRVS ETEYSALEQN 


TK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Investigation of the role of rat RDC-1 as a putative calcitonin gene-related peptide receptor." Mirtella A., Lowe S.R., Bartlett T.J., Drake W., Clark A.J. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	Xie P., Fu A.K.Y., Ip N.Y. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Regional and cellular localization of the CXCl12/SDF-1 chemokine receptor CXCR7 in the developing and adult rat brain." Schonemeier B., Kolodziej A., Schulz S., Jacobs S., Hoellt V., Stumm R. J. Comp. Neurol. 510:207-220(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Enhanced expression of the CXCl12/SDF-1 chemokine receptor CXCR7 after cerebral ischemia in the rat brain." Schonemeier B., Schulz S., Hoellt V., Stumm R. J. Neuroimmunol. 198:39-45(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]	"Beta-arrestin- but not G protein-mediated signaling by the 'decoy' receptor CXCR7." Rajagopal S., Kim J., Ahn S., Craig S., Lam C.M., Gerard N.P., Gerard C., Lefkowitz R.J. Proc. Natl. Acad. Sci. U.S.A. 107:628-632(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"CXCR7 protein expression in human adult brain and differentiated neurons." Shimizu S., Brown M., Sengupta R., Penfold M.E., Meucci O. PLoS ONE 6:E20680-E20680(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010828 mRNA. Translation: CAA09370.1. AF118816 mRNA. Translation: AAF34338.1. CH473997 Genomic DNA. Translation: EDL92073.1. CH473997 Genomic DNA. Translation: EDL92074.1.
IPI	IPI00204223. IPI00209368.
RefSeq	NP_445804.1. NM_053352.1.
UniGene	Rn.12959.
3D structure databases
ProteinModelPortal	O89039.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026558.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O89039.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026558; ENSRNOP00000026558; ENSRNOG00000019622.
GeneID	84348.
KEGG	rno:84348.
UCSC	RGD:620601. rat.
Organism-specific databases
CTD	57007.
RGD	620601. Cxcr7.
Phylogenomic databases
eggNOG	NOG306050.
GeneTree	ENSGT00700000104167.
HOGENOM	HOG000261660.
HOVERGEN	HBG106832.
InParanoid	Q9JLZ0.
KO	K04304.
OMA	YIPFTCQ.
OrthoDB	EOG45DWPR.
Gene expression databases
ArrayExpress	O89039.
Genevestigator	O89039.
GermOnline	ENSRNOG00000019622. Rattus norvegicus.
Family and domain databases
InterPro	IPR001416. Chemokine_CXCR7. IPR000355. Chemokine_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24227. PTHR24227. 1 hit. PTHR24227:SF34. PTHR24227:SF34. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00646. RDC1ORPHANR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616647.

Entry information

Entry name

CXCR7_RAT

Accession

Primary (citable) accession number: O89039
Secondary accession number(s): Q9JLZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 25, 2012
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents