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Protein

Matrilin-4

Gene

Matn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major component of the extracellular matrix of cartilage.

GO - Molecular functioni

GO - Biological processi

  • response to axon injury Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Matrilin-4
Short name:
MAT-4
Gene namesi
Name:Matn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1328314. Matn4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 624603Matrilin-4PRO_0000007661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Disulfide bondi221 ↔ 232By similarity
Disulfide bondi228 ↔ 241By similarity
Disulfide bondi243 ↔ 256By similarity
Disulfide bondi262 ↔ 273By similarity
Disulfide bondi269 ↔ 282By similarity
Disulfide bondi284 ↔ 297By similarity
Disulfide bondi303 ↔ 314By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis
Disulfide bondi310 ↔ 323By similarity
Disulfide bondi325 ↔ 338By similarity
Disulfide bondi344 ↔ 355By similarity
Disulfide bondi351 ↔ 364By similarity
Disulfide bondi366 ↔ 379By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO89029.
PaxDbiO89029.
PRIDEiO89029.

PTM databases

PhosphoSiteiO89029.

Expressioni

Tissue specificityi

Lung, brain, sternum, kidney and heart.

Developmental stagei

The short isoform was detected in 7 weeks old mice but not in developing mice (19.5 dpc embryos or in 2, 8, and 21 days old animals).

Gene expression databases

CleanExiMM_MATN4.

Interactioni

Subunit structurei

Interacts with COMP.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099392.

Structurei

3D structure databases

ProteinModelPortaliO89029.
SMRiO89029. Positions 21-215, 231-339, 347-603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 215180VWFA 1PROSITE-ProRule annotationAdd
BLAST
Domaini217 – 25741EGF-like 1Add
BLAST
Domaini258 – 29841EGF-like 2Add
BLAST
Domaini299 – 33941EGF-like 3Add
BLAST
Domaini340 – 38041EGF-like 4Add
BLAST
Domaini388 – 563176VWFA 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili590 – 62334Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 4 EGF-like domains.Curated
Contains 2 VWFA domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR74. Eukaryota.
ENOG410XR1E. LUCA.
HOGENOMiHOG000263415.
HOVERGENiHBG056906.
InParanoidiO89029.
PhylomeDBiO89029.

Family and domain databases

Gene3Di1.20.5.30. 1 hit.
3.40.50.410. 2 hits.
InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR009030. Growth_fac_rcpt_.
IPR030765. Matrilin-3/4.
IPR019466. Matrilin_coiled-coil_trimer.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR11132:SF50. PTHR11132:SF50. 1 hit.
PfamiPF10393. Matrilin_ccoil. 1 hit.
PF00092. VWA. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 4 hits.
SM01279. Matrilin_ccoil. 1 hit.
SM00327. VWA. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 2 hits.
SSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 2 hits.
PS50234. VWFA. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: O89029-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGPCCWPLS LLLLFLQSWE TQLQSAGPRC YNGPLDLVFM IDSSRSVRPF
60 70 80 90 100
EFETMRQFLV GLLRSLDVGL NATRVGVIQY SSQVQSVFPL GAFSRREDME
110 120 130 140 150
RAIRAVVPLA QGTMTGLAIQ YAMNVAFSEA EGARPSEERV PRVLVIVTDG
160 170 180 190 200
RPQDRVAEVA AQARARGIEI YAVGVQRADV GSLRTMASPP LDQHVFLVES
210 220 230 240 250
FDLIQEFGLQ FQGRLCGKDL CAELVHGCQH LCVNAPGTFY CACNSGYKLA
260 270 280 290 300
PDNKNCLALD LCAEGTHGCE HLCVNSVDSY FCRCRAGFAL QQDQRSCRAI
310 320 330 340 350
DYCSFGNHSC QHECVSTLAG PQCRCREGHD LLPDGRSCRV RDFCNGVDHG
360 370 380 390 400
CEFQCVSEGL SFHCLCPEGR RLQADGKSCD RCREGHVDLV LLVDGSKSVR
410 420 430 440 450
PQNFELVKRF VNQIVDFLDV SPEGTRVGLV QFSSRVRTEF PLGRYGTAAE
460 470 480 490 500
VKQAVLAVEY MERGTMTGLA LRHMVEHSFS EAQGARPRDL NVPRVGLVFT
510 520 530 540 550
DGRSQDDISV WAARAKEEGI VMYAVGVGKA VEEELREIAS EPSELHVSYS
560 570 580 590 600
PDFSTMTHLL ENLKGSICPE EGIGAGTELR SPCECESLVE FQGRTLGALE
610 620
SLTQNLARLT ERLEELENQL ASRK
Length:624
Mass (Da):68,918
Last modified:November 1, 1998 - v1
Checksum:iDFA28D2C94B1A14F
GO
Isoform Short (identifier: O89029-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-217: Missing.

Show »
Length:434
Mass (Da):47,944
Checksum:i2F2900295D389F2F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti319 – 3191A → E in strain: C57BL/6J.
Natural varianti346 – 3461G → D in strain: C57BL/6J.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 217190Missing in isoform Short. 1 PublicationVSP_001401Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006140 mRNA. Translation: CAA06889.1.
AJ006140 mRNA. Translation: CAA06890.1.
BC036558 mRNA. Translation: AAH36558.1.
AJ010984 Genomic DNA. Translation: CAA09451.1.
CCDSiCCDS17034.1. [O89029-1]
RefSeqiNP_001239492.1. NM_001252563.1.
UniGeneiMm.29428.

Genome annotation databases

GeneIDi17183.
KEGGimmu:17183.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006140 mRNA. Translation: CAA06889.1.
AJ006140 mRNA. Translation: CAA06890.1.
BC036558 mRNA. Translation: AAH36558.1.
AJ010984 Genomic DNA. Translation: CAA09451.1.
CCDSiCCDS17034.1. [O89029-1]
RefSeqiNP_001239492.1. NM_001252563.1.
UniGeneiMm.29428.

3D structure databases

ProteinModelPortaliO89029.
SMRiO89029. Positions 21-215, 231-339, 347-603.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099392.

PTM databases

PhosphoSiteiO89029.

Proteomic databases

MaxQBiO89029.
PaxDbiO89029.
PRIDEiO89029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17183.
KEGGimmu:17183.

Organism-specific databases

CTDi8785.
MGIiMGI:1328314. Matn4.

Phylogenomic databases

eggNOGiENOG410IR74. Eukaryota.
ENOG410XR1E. LUCA.
HOGENOMiHOG000263415.
HOVERGENiHBG056906.
InParanoidiO89029.
PhylomeDBiO89029.

Miscellaneous databases

PROiO89029.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MATN4.

Family and domain databases

Gene3Di1.20.5.30. 1 hit.
3.40.50.410. 2 hits.
InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR009030. Growth_fac_rcpt_.
IPR030765. Matrilin-3/4.
IPR019466. Matrilin_coiled-coil_trimer.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR11132:SF50. PTHR11132:SF50. 1 hit.
PfamiPF10393. Matrilin_ccoil. 1 hit.
PF00092. VWA. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 4 hits.
SM01279. Matrilin_ccoil. 1 hit.
SM00327. VWA. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 2 hits.
SSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 2 hits.
PS50234. VWFA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Matrilin-4, a new member of the matrilin family of extracellular matrix proteins."
    Wagener R., Kobbe B., Paulsson M.
    FEBS Lett. 436:123-127(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), VARIANTS.
    Strain: C57BL/6J and CD-1.
    Tissue: Fetus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  3. "Genomic organisation, alternative splicing and primary structure of human matrilin-4."
    Wagener R., Kobbe B., Paulsson M.
    FEBS Lett. 438:165-170(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-257.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiMATN4_MOUSE
AccessioniPrimary (citable) accession number: O89029
Secondary accession number(s): O89030, Q9QWS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.