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O89026

- ROBO1_MOUSE

UniProt

O89026 - ROBO1_MOUSE

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Protein

Roundabout homolog 1

Gene

Robo1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). May be required for lung development.By similarity1 Publication

GO - Biological processi

  1. axon guidance Source: MGI
  2. axon midline choice point recognition Source: UniProtKB
  3. mammary duct terminal end bud growth Source: MGI
  4. negative regulation of cell proliferation Source: MGI
  5. olfactory bulb interneuron development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Chemotaxis, Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Roundabout homolog 1
Gene namesi
Name:Robo1
Synonyms:Dutt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1274781. Robo1.

Subcellular locationi

GO - Cellular componenti

  1. axolemma Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show defects in commissural axon guidance in spinal chord including midline recrossing and an altered lateral and ventral funiculi projection. The phenotype resembles that of a SLIT1;SLIT2;SLIT3 triple mutant. They also mimick a naturally occurring human homozygous deletion mutant detected in a small lung cancer cell line, frequently die at birth by respiratory failure with accompanying abnormal lung histology. Surviving mice develop bronchial hyperplasia.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 16121593Roundabout homolog 1PRO_0000031034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 108PROSITE-ProRule annotation
Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi152 ↔ 201PROSITE-ProRule annotation
Disulfide bondi244 ↔ 291PROSITE-ProRule annotation
Disulfide bondi333 ↔ 389PROSITE-ProRule annotation
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi437 ↔ 486PROSITE-ProRule annotation
Glycosylationi751 – 7511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi781 – 7811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi788 – 7881N-linked (GlcNAc...)Sequence Analysis
Modified residuei901 – 9011PhosphoserineBy similarity
Modified residuei999 – 9991PhosphotyrosineBy similarity
Modified residuei1016 – 10161PhosphoserineBy similarity
Modified residuei1034 – 10341PhosphotyrosineBy similarity
Modified residuei1075 – 10751PhosphotyrosineBy similarity
Modified residuei1201 – 12011PhosphothreonineBy similarity
Modified residuei1258 – 12581PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated. May be deubiquitinated by USP33.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO89026.
PaxDbiO89026.
PRIDEiO89026.

PTM databases

PhosphoSiteiO89026.

Expressioni

Tissue specificityi

Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways.2 Publications

Developmental stagei

Earliest and highest expression at 11 dpc. Expression is detected in developing somits, brain, neural tube, and pericardiac mesenchyme. By in situ hybridization is detected in marginal zones bordering the mitotically active periventricular region, weakly extending to the ventral aspect impinging on motor neuron columns. Also detected in the ventral third of the developing neural tube, and in spinal chord throughout the full length of the neural tube. Also detected at 17.5 dpc in lung mesenchyme. Expressed at E11.5 in spinal chord, predominantly localized to postcrossing commissural axons.3 Publications

Gene expression databases

CleanExiMM_ROBO1.
GenevestigatoriO89026.

Interactioni

Subunit structurei

Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding (By similarity). Interacts with SLIT1 and SLIT2.By similarity3 Publications

Protein-protein interaction databases

BioGridi202949. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliO89026.
SMRiO89026. Positions 22-846.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 858839ExtracellularSequence AnalysisAdd
BLAST
Topological domaini880 – 1612733CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei859 – 87921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 12597Ig-like C2-type 1Add
BLAST
Domaini131 – 21888Ig-like C2-type 2Add
BLAST
Domaini223 – 30785Ig-like C2-type 3Add
BLAST
Domaini312 – 40796Ig-like C2-type 4Add
BLAST
Domaini416 – 50287Ig-like C2-type 5Add
BLAST
Domaini524 – 61895Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini637 – 73498Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini739 – 83597Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. ROBO family.Curated
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG238978.
HOGENOMiHOG000010267.
HOVERGENiHBG073476.
InParanoidiO89026.
KOiK06753.
PhylomeDBiO89026.

Family and domain databases

Gene3Di2.60.40.10. 9 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR003596. Ig_V-set_subgr.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
SM00408. IGc2. 4 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89026 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC
60 70 80 90 100
KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR
110 120 130 140 150
PDEGVYICVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM
160 170 180 190 200
ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV
210 220 230 240 250
CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP
260 270 280 290 300
VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA
310 320 330 340 350
EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS
360 370 380 390 400
QNLLFSYQPP QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT
410 420 430 440 450
KAYLEVTDVI ADRPPPVIRQ GPVNQTVAVD GTLILSCVAT GSPAPTILWR
460 470 480 490 500
KDGVLVSTQD SRIKQLESGV LQIRYAKLGD TGRYTCTAST PSGEATWSAY
510 520 530 540 550
IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL SWQPNLNSGA
560 570 580 590 600
TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA
610 620 630 640 650
YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS
660 670 680 690 700
SVEVHWTVDQ QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP
710 720 730 740 750
DLRKGVNYEI KARPFFNEFQ GADSEIKFAK TLEEAPSAPP RSVTVSKNDG
760 770 780 790 800
NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG NETKYHINKT VDGSTFSVVI
810 820 830 840 850
PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS PEDQVSLAQQ
860 870 880 890 900
ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP
910 920 930 940 950
SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND
960 970 980 990 1000
CSINCCTAGN GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG
1010 1020 1030 1040 1050
DVDLSNKINE MKTFNSPNLK DGRFVNPSGQ PTPYATTQLI QANLSNNMNN
1060 1070 1080 1090 1100
GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ NKLNKDYRAN DTIPPTIPYN
1110 1120 1130 1140 1150
QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG MNWADLLPPP
1160 1170 1180 1190 1200
PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP
1210 1220 1230 1240 1250
TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP
1260 1270 1280 1290 1300
DRRRQPVSPP PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA
1310 1320 1330 1340 1350
KMQTRRLLLR GLEQTPASSV GDLESSVTGS MINGWGSASE EDNISSGRSS
1360 1370 1380 1390 1400
VSSSDGSFFT DADFAQAVAA AAEYAGLKVA RRQMQDAAGR RHFHASQCPR
1410 1420 1430 1440 1450
PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD LPPPPVPPPA
1460 1470 1480 1490 1500
IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ
1510 1520 1530 1540 1550
VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR
1560 1570 1580 1590 1600
PTFPTSNNPR DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG
1610
DENNEELEET ES
Length:1,612
Mass (Da):176,407
Last modified:November 1, 1998 - v1
Checksum:i5F2988C544796B4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17793 mRNA. Translation: CAA76850.1.
CCDSiCCDS37376.1.
PIRiT30805.
RefSeqiNP_062286.2. NM_019413.2.
UniGeneiMm.310772.

Genome annotation databases

GeneIDi19876.
KEGGimmu:19876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17793 mRNA. Translation: CAA76850.1 .
CCDSi CCDS37376.1.
PIRi T30805.
RefSeqi NP_062286.2. NM_019413.2.
UniGenei Mm.310772.

3D structure databases

ProteinModelPortali O89026.
SMRi O89026. Positions 22-846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202949. 4 interactions.

PTM databases

PhosphoSitei O89026.

Proteomic databases

MaxQBi O89026.
PaxDbi O89026.
PRIDEi O89026.

Protocols and materials databases

DNASUi 19876.
Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 19876.
KEGGi mmu:19876.

Organism-specific databases

CTDi 6091.
MGIi MGI:1274781. Robo1.

Phylogenomic databases

eggNOGi NOG238978.
HOGENOMi HOG000010267.
HOVERGENi HBG073476.
InParanoidi O89026.
KOi K06753.
PhylomeDBi O89026.

Miscellaneous databases

NextBioi 297362.
PROi O89026.
SOURCEi Search...

Gene expression databases

CleanExi MM_ROBO1.
Genevestigatori O89026.

Family and domain databases

Gene3Di 2.60.40.10. 9 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR003596. Ig_V-set_subgr.
[Graphical view ]
Pfami PF00041. fn3. 3 hits.
PF07679. I-set. 5 hits.
[Graphical view ]
SMARTi SM00060. FN3. 3 hits.
SM00408. IGc2. 4 hits.
SM00406. IGv. 1 hit.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
PROSITEi PS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mouse homologue of human DUTT1/H-robo1 gene: protein sequence and chromosomal location."
    Wu M.C., Lowe N., Fordham R., Rabbitts P.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Dutt1/Robo1 gene."
    Xian J., Clark K.J., Fordham R., Pannell R., Rabbitts T.H., Rabbitts P.H.
    Proc. Natl. Acad. Sci. U.S.A. 98:15062-15066(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION, DISRUPTION PHENOTYPE.
  3. "Conserved roles for slit and robo proteins in midline commissural axon guidance."
    Long H., Sabatier C., Ma L., Plump A., Yuan W., Ornitz D.M., Tamada A., Murakami F., Goodman C.S., Tessier-Lavigne M.
    Neuron 42:213-223(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "The DUTT1 gene, a novel NCAM family member is expressed in developing murine neural tissues and has an unusually broad pattern of expression."
    Sundaresan V., Roberts I., Bateman A., Bankier A., Sheppard M., Hobbs C., Xiong J., Minna J., Latif F., Lerman M., Rabbitts P.
    Mol. Cell. Neurosci. 11:29-35(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance."
    Yuan W., Zhou L., Chen J.H., Wu J.Y., Rao Y., Ornitz D.M.
    Dev. Biol. 212:290-306(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLIT1.
  6. "Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development."
    Clark K., Hammond E., Rabbitts P.
    FEBS Lett. 523:12-16(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Midline crossing and Slit responsiveness of commissural axons require USP33."
    Yuasa-Kawada J., Kinoshita-Kawada M., Wu G., Rao Y., Wu J.Y.
    Nat. Neurosci. 12:1087-1089(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH USP33.
  8. "Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration."
    Yuasa-Kawada J., Kinoshita-Kawada M., Rao Y., Wu J.Y.
    Proc. Natl. Acad. Sci. U.S.A. 106:14530-14535(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH USP33.

Entry informationi

Entry nameiROBO1_MOUSE
AccessioniPrimary (citable) accession number: O89026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3