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O89026 (ROBO1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Roundabout homolog 1
Gene names
Name:Robo1
Synonyms:Dutt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1612 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex By similarity. May be required for lung development. Ref.2 Ref.3

Subunit structure

Interacts with SLIT1 and SLIT2. Ref.5 Ref.7 Ref.8

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways. Ref.3 Ref.6

Developmental stage

Earliest and highest expression at 11 dpc. Expression is detected in developing somits, brain, neural tube, and pericardiac mesenchyme. By in situ hybridization is detected in marginal zones bordering the mitotically active periventricular region, weakly extending to the ventral aspect impinging on motor neuron columns. Also detected in the ventral third of the developing neural tube, and in spinal chord throughout the full length of the neural tube. Also detected at 17.5 dpc in lung mesenchyme. Expressed at E11.5 in spinal chord, predominantly localized to postcrossing commissural axons. Ref.3 Ref.4 Ref.6

Post-translational modification

Ubiquitinated. May be deubiquitinated by USP33. Ref.7

Disruption phenotype

Mice show defects in commissural axon guidance in spinal chord including midline recrossing and an altered lateral and ventral funiculi projection. The phenotype resembles that of a SLIT1;SLIT2;SLIT3 triple mutant. They also mimick a naturally occurring human homozygous deletion mutant detected in a small lung cancer cell line, frequently die at birth by respiratory failure with accompanying abnormal lung histology. Surviving mice develop bronchial hyperplasia. Ref.2

Sequence similarities

Belongs to the immunoglobulin superfamily. ROBO family.

Contains 3 fibronectin type-III domains.

Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 16121593Roundabout homolog 1
PRO_0000031034

Regions

Topological domain20 – 858839Extracellular Potential
Transmembrane859 – 87921Helical; Potential
Topological domain880 – 1612733Cytoplasmic Potential
Domain29 – 12597Ig-like C2-type 1
Domain131 – 21888Ig-like C2-type 2
Domain223 – 30785Ig-like C2-type 3
Domain312 – 40796Ig-like C2-type 4
Domain416 – 50287Ig-like C2-type 5
Domain524 – 61895Fibronectin type-III 1
Domain637 – 73498Fibronectin type-III 2
Domain739 – 83597Fibronectin type-III 3

Amino acid modifications

Modified residue9011Phosphoserine By similarity
Modified residue9991Phosphotyrosine By similarity
Modified residue10161Phosphoserine By similarity
Modified residue10341Phosphotyrosine By similarity
Modified residue10751Phosphotyrosine By similarity
Modified residue12011Phosphothreonine By similarity
Modified residue12581Phosphoserine By similarity
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation7511N-linked (GlcNAc...) Potential
Glycosylation7811N-linked (GlcNAc...) Potential
Glycosylation7881N-linked (GlcNAc...) Potential
Disulfide bond50 ↔ 108 Potential
Disulfide bond152 ↔ 201 Potential
Disulfide bond244 ↔ 291 Potential
Disulfide bond333 ↔ 389 Potential
Disulfide bond437 ↔ 486 Potential

Sequences

Sequence LengthMass (Da)Tools
O89026 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 5F2988C544796B4B

FASTA1,612176,407
        10         20         30         40         50         60 
MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC KAEGRPTPTI 

        70         80         90        100        110        120 
EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR PDEGVYICVA RNYLGEAVSH 

       130        140        150        160        170        180 
NASLEVAILR DDFRQNPSDV MVAVGEPAVM ECQPPRGHPE PTISWKKDGS PLDDKDERIT 

       190        200        210        220        230        240 
IRGGKLMITY TRKSDAGKYV CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA 

       250        260        270        280        290        300 
EFKCEARGDP VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA 

       310        320        330        340        350        360 
EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS QNLLFSYQPP 

       370        380        390        400        410        420 
QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT KAYLEVTDVI ADRPPPVIRQ 

       430        440        450        460        470        480 
GPVNQTVAVD GTLILSCVAT GSPAPTILWR KDGVLVSTQD SRIKQLESGV LQIRYAKLGD 

       490        500        510        520        530        540 
TGRYTCTAST PSGEATWSAY IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL 

       550        560        570        580        590        600 
SWQPNLNSGA TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA 

       610        620        630        640        650        660 
YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS SVEVHWTVDQ 

       670        680        690        700        710        720 
QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP DLRKGVNYEI KARPFFNEFQ 

       730        740        750        760        770        780 
GADSEIKFAK TLEEAPSAPP RSVTVSKNDG NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG 

       790        800        810        820        830        840 
NETKYHINKT VDGSTFSVVI PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS 

       850        860        870        880        890        900 
PEDQVSLAQQ ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP 

       910        920        930        940        950        960 
SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND CSINCCTAGN 

       970        980        990       1000       1010       1020 
GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG DVDLSNKINE MKTFNSPNLK 

      1030       1040       1050       1060       1070       1080 
DGRFVNPSGQ PTPYATTQLI QANLSNNMNN GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ 

      1090       1100       1110       1120       1130       1140 
NKLNKDYRAN DTIPPTIPYN QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG 

      1150       1160       1170       1180       1190       1200 
MNWADLLPPP PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP 

      1210       1220       1230       1240       1250       1260 
TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP DRRRQPVSPP 

      1270       1280       1290       1300       1310       1320 
PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA KMQTRRLLLR GLEQTPASSV 

      1330       1340       1350       1360       1370       1380 
GDLESSVTGS MINGWGSASE EDNISSGRSS VSSSDGSFFT DADFAQAVAA AAEYAGLKVA 

      1390       1400       1410       1420       1430       1440 
RRQMQDAAGR RHFHASQCPR PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD 

      1450       1460       1470       1480       1490       1500 
LPPPPVPPPA IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ 

      1510       1520       1530       1540       1550       1560 
VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR PTFPTSNNPR 

      1570       1580       1590       1600       1610 
DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG DENNEELEET ES 

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References

[1]"The mouse homologue of human DUTT1/H-robo1 gene: protein sequence and chromosomal location."
Wu M.C., Lowe N., Fordham R., Rabbitts P.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Dutt1/Robo1 gene."
Xian J., Clark K.J., Fordham R., Pannell R., Rabbitts T.H., Rabbitts P.H.
Proc. Natl. Acad. Sci. U.S.A. 98:15062-15066(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION, DISRUPTION PHENOTYPE.
[3]"Conserved roles for slit and robo proteins in midline commissural axon guidance."
Long H., Sabatier C., Ma L., Plump A., Yuan W., Ornitz D.M., Tamada A., Murakami F., Goodman C.S., Tessier-Lavigne M.
Neuron 42:213-223(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]"The DUTT1 gene, a novel NCAM family member is expressed in developing murine neural tissues and has an unusually broad pattern of expression."
Sundaresan V., Roberts I., Bateman A., Bankier A., Sheppard M., Hobbs C., Xiong J., Minna J., Latif F., Lerman M., Rabbitts P.
Mol. Cell. Neurosci. 11:29-35(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance."
Yuan W., Zhou L., Chen J.H., Wu J.Y., Rao Y., Ornitz D.M.
Dev. Biol. 212:290-306(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLIT1.
[6]"Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development."
Clark K., Hammond E., Rabbitts P.
FEBS Lett. 523:12-16(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]"Midline crossing and Slit responsiveness of commissural axons require USP33."
Yuasa-Kawada J., Kinoshita-Kawada M., Wu G., Rao Y., Wu J.Y.
Nat. Neurosci. 12:1087-1089(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION, INTERACTION WITH USP33.
[8]"Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration."
Yuasa-Kawada J., Kinoshita-Kawada M., Rao Y., Wu J.Y.
Proc. Natl. Acad. Sci. U.S.A. 106:14530-14535(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH USP33.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y17793 mRNA. Translation: CAA76850.1.
PIRT30805.
RefSeqNP_062286.2. NM_019413.2.
UniGeneMm.310772.

3D structure databases

ProteinModelPortalO89026.
SMRO89026. Positions 5-832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202949. 4 interactions.

PTM databases

PhosphoSiteO89026.

Proteomic databases

PaxDbO89026.
PRIDEO89026.

Protocols and materials databases

DNASU19876.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID19876.
KEGGmmu:19876.

Organism-specific databases

CTD6091.
MGIMGI:1274781. Robo1.

Phylogenomic databases

eggNOGNOG238978.
HOGENOMHOG000010267.
HOVERGENHBG073476.
InParanoidO89026.
KOK06753.
PhylomeDBO89026.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

CleanExMM_ROBO1.
GenevestigatorO89026.

Family and domain databases

Gene3D2.60.40.10. 9 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR003596. Ig_V-set_subgr.
[Graphical view]
PfamPF00041. fn3. 3 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTSM00060. FN3. 3 hits.
SM00408. IGc2. 4 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio297362.
PROO89026.
SOURCESearch...

Entry information

Entry nameROBO1_MOUSE
AccessionPrimary (citable) accession number: O89026
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot