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O89026

- ROBO1_MOUSE

UniProt

O89026 - ROBO1_MOUSE

Protein

Roundabout homolog 1

Gene

Robo1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex By similarity. May be required for lung development.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein heterodimerization activity Source: MGI

    GO - Biological processi

    1. axon guidance Source: MGI
    2. axon midline choice point recognition Source: UniProtKB
    3. mammary duct terminal end bud growth Source: MGI
    4. negative regulation of cell proliferation Source: MGI
    5. olfactory bulb interneuron development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Receptor

    Keywords - Biological processi

    Chemotaxis, Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Roundabout homolog 1
    Gene namesi
    Name:Robo1
    Synonyms:Dutt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1274781. Robo1.

    Subcellular locationi

    GO - Cellular componenti

    1. axolemma Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice show defects in commissural axon guidance in spinal chord including midline recrossing and an altered lateral and ventral funiculi projection. The phenotype resembles that of a SLIT1;SLIT2;SLIT3 triple mutant. They also mimick a naturally occurring human homozygous deletion mutant detected in a small lung cancer cell line, frequently die at birth by respiratory failure with accompanying abnormal lung histology. Surviving mice develop bronchial hyperplasia.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 16121593Roundabout homolog 1PRO_0000031034Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 108PROSITE-ProRule annotation
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi152 ↔ 201PROSITE-ProRule annotation
    Disulfide bondi244 ↔ 291PROSITE-ProRule annotation
    Disulfide bondi333 ↔ 389PROSITE-ProRule annotation
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi437 ↔ 486PROSITE-ProRule annotation
    Glycosylationi751 – 7511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi781 – 7811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi788 – 7881N-linked (GlcNAc...)Sequence Analysis
    Modified residuei901 – 9011PhosphoserineBy similarity
    Modified residuei999 – 9991PhosphotyrosineBy similarity
    Modified residuei1016 – 10161PhosphoserineBy similarity
    Modified residuei1034 – 10341PhosphotyrosineBy similarity
    Modified residuei1075 – 10751PhosphotyrosineBy similarity
    Modified residuei1201 – 12011PhosphothreonineBy similarity
    Modified residuei1258 – 12581PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated. May be deubiquitinated by USP33.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO89026.
    PaxDbiO89026.
    PRIDEiO89026.

    PTM databases

    PhosphoSiteiO89026.

    Expressioni

    Tissue specificityi

    Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways.2 Publications

    Developmental stagei

    Earliest and highest expression at 11 dpc. Expression is detected in developing somits, brain, neural tube, and pericardiac mesenchyme. By in situ hybridization is detected in marginal zones bordering the mitotically active periventricular region, weakly extending to the ventral aspect impinging on motor neuron columns. Also detected in the ventral third of the developing neural tube, and in spinal chord throughout the full length of the neural tube. Also detected at 17.5 dpc in lung mesenchyme. Expressed at E11.5 in spinal chord, predominantly localized to postcrossing commissural axons.3 Publications

    Gene expression databases

    CleanExiMM_ROBO1.
    GenevestigatoriO89026.

    Interactioni

    Subunit structurei

    Interacts with SLIT1 and SLIT2.3 Publications

    Protein-protein interaction databases

    BioGridi202949. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO89026.
    SMRiO89026. Positions 22-846.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 858839ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini880 – 1612733CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei859 – 87921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 12597Ig-like C2-type 1Add
    BLAST
    Domaini131 – 21888Ig-like C2-type 2Add
    BLAST
    Domaini223 – 30785Ig-like C2-type 3Add
    BLAST
    Domaini312 – 40796Ig-like C2-type 4Add
    BLAST
    Domaini416 – 50287Ig-like C2-type 5Add
    BLAST
    Domaini524 – 61895Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini637 – 73498Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini739 – 83597Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the immunoglobulin superfamily. ROBO family.Curated
    Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG238978.
    HOGENOMiHOG000010267.
    HOVERGENiHBG073476.
    InParanoidiO89026.
    KOiK06753.
    PhylomeDBiO89026.

    Family and domain databases

    Gene3Di2.60.40.10. 9 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003598. Ig_sub2.
    IPR003596. Ig_V-set_subgr.
    [Graphical view]
    PfamiPF00041. fn3. 3 hits.
    PF07679. I-set. 5 hits.
    [Graphical view]
    SMARTiSM00060. FN3. 3 hits.
    SM00408. IGc2. 4 hits.
    SM00406. IGv. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    PROSITEiPS50853. FN3. 3 hits.
    PS50835. IG_LIKE. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O89026-1 [UniParc]FASTAAdd to Basket

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    MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC     50
    KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR 100
    PDEGVYICVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM 150
    ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV 200
    CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP 250
    VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA 300
    EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS 350
    QNLLFSYQPP QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT 400
    KAYLEVTDVI ADRPPPVIRQ GPVNQTVAVD GTLILSCVAT GSPAPTILWR 450
    KDGVLVSTQD SRIKQLESGV LQIRYAKLGD TGRYTCTAST PSGEATWSAY 500
    IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL SWQPNLNSGA 550
    TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA 600
    YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS 650
    SVEVHWTVDQ QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP 700
    DLRKGVNYEI KARPFFNEFQ GADSEIKFAK TLEEAPSAPP RSVTVSKNDG 750
    NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG NETKYHINKT VDGSTFSVVI 800
    PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS PEDQVSLAQQ 850
    ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP 900
    SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND 950
    CSINCCTAGN GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG 1000
    DVDLSNKINE MKTFNSPNLK DGRFVNPSGQ PTPYATTQLI QANLSNNMNN 1050
    GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ NKLNKDYRAN DTIPPTIPYN 1100
    QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG MNWADLLPPP 1150
    PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP 1200
    TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP 1250
    DRRRQPVSPP PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA 1300
    KMQTRRLLLR GLEQTPASSV GDLESSVTGS MINGWGSASE EDNISSGRSS 1350
    VSSSDGSFFT DADFAQAVAA AAEYAGLKVA RRQMQDAAGR RHFHASQCPR 1400
    PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD LPPPPVPPPA 1450
    IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ 1500
    VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR 1550
    PTFPTSNNPR DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG 1600
    DENNEELEET ES 1612
    Length:1,612
    Mass (Da):176,407
    Last modified:November 1, 1998 - v1
    Checksum:i5F2988C544796B4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17793 mRNA. Translation: CAA76850.1.
    CCDSiCCDS37376.1.
    PIRiT30805.
    RefSeqiNP_062286.2. NM_019413.2.
    UniGeneiMm.310772.

    Genome annotation databases

    GeneIDi19876.
    KEGGimmu:19876.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17793 mRNA. Translation: CAA76850.1 .
    CCDSi CCDS37376.1.
    PIRi T30805.
    RefSeqi NP_062286.2. NM_019413.2.
    UniGenei Mm.310772.

    3D structure databases

    ProteinModelPortali O89026.
    SMRi O89026. Positions 22-846.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202949. 4 interactions.

    PTM databases

    PhosphoSitei O89026.

    Proteomic databases

    MaxQBi O89026.
    PaxDbi O89026.
    PRIDEi O89026.

    Protocols and materials databases

    DNASUi 19876.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 19876.
    KEGGi mmu:19876.

    Organism-specific databases

    CTDi 6091.
    MGIi MGI:1274781. Robo1.

    Phylogenomic databases

    eggNOGi NOG238978.
    HOGENOMi HOG000010267.
    HOVERGENi HBG073476.
    InParanoidi O89026.
    KOi K06753.
    PhylomeDBi O89026.

    Miscellaneous databases

    NextBioi 297362.
    PROi O89026.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ROBO1.
    Genevestigatori O89026.

    Family and domain databases

    Gene3Di 2.60.40.10. 9 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003598. Ig_sub2.
    IPR003596. Ig_V-set_subgr.
    [Graphical view ]
    Pfami PF00041. fn3. 3 hits.
    PF07679. I-set. 5 hits.
    [Graphical view ]
    SMARTi SM00060. FN3. 3 hits.
    SM00408. IGc2. 4 hits.
    SM00406. IGv. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    PROSITEi PS50853. FN3. 3 hits.
    PS50835. IG_LIKE. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse homologue of human DUTT1/H-robo1 gene: protein sequence and chromosomal location."
      Wu M.C., Lowe N., Fordham R., Rabbitts P.
      Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Dutt1/Robo1 gene."
      Xian J., Clark K.J., Fordham R., Pannell R., Rabbitts T.H., Rabbitts P.H.
      Proc. Natl. Acad. Sci. U.S.A. 98:15062-15066(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION, DISRUPTION PHENOTYPE.
    3. "Conserved roles for slit and robo proteins in midline commissural axon guidance."
      Long H., Sabatier C., Ma L., Plump A., Yuan W., Ornitz D.M., Tamada A., Murakami F., Goodman C.S., Tessier-Lavigne M.
      Neuron 42:213-223(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    4. "The DUTT1 gene, a novel NCAM family member is expressed in developing murine neural tissues and has an unusually broad pattern of expression."
      Sundaresan V., Roberts I., Bateman A., Bankier A., Sheppard M., Hobbs C., Xiong J., Minna J., Latif F., Lerman M., Rabbitts P.
      Mol. Cell. Neurosci. 11:29-35(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance."
      Yuan W., Zhou L., Chen J.H., Wu J.Y., Rao Y., Ornitz D.M.
      Dev. Biol. 212:290-306(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLIT1.
    6. "Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development."
      Clark K., Hammond E., Rabbitts P.
      FEBS Lett. 523:12-16(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. "Midline crossing and Slit responsiveness of commissural axons require USP33."
      Yuasa-Kawada J., Kinoshita-Kawada M., Wu G., Rao Y., Wu J.Y.
      Nat. Neurosci. 12:1087-1089(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION, INTERACTION WITH USP33.
    8. "Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration."
      Yuasa-Kawada J., Kinoshita-Kawada M., Rao Y., Wu J.Y.
      Proc. Natl. Acad. Sci. U.S.A. 106:14530-14535(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH USP33.

    Entry informationi

    Entry nameiROBO1_MOUSE
    AccessioniPrimary (citable) accession number: O89026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2004
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3