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Protein

Tripeptidyl-peptidase 1

Gene

Tpp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei271Charge relay systemBy similarity1
Active sitei275Charge relay systemBy similarity1
Active sitei474Charge relay systemBy similarity1
Metal bindingi516CalciumBy similarity1
Metal bindingi517Calcium; via carbonyl oxygenBy similarity1
Metal bindingi538Calcium; via carbonyl oxygenBy similarity1
Metal bindingi540Calcium; via carbonyl oxygenBy similarity1
Metal bindingi542CalciumBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: UniProtKB
  • tripeptidyl-peptidase activity Source: UniProtKB

GO - Biological processi

  • bone resorption Source: UniProtKB
  • central nervous system development Source: GO_Central
  • epithelial cell differentiation Source: Ensembl
  • lysosome organization Source: MGI
  • nervous system development Source: UniProtKB
  • neuromuscular process controlling balance Source: MGI
  • peptide catabolic process Source: UniProtKB
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS53.003

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 1 (EC:3.4.14.9)
Short name:
TPP-1
Alternative name(s):
Lysosomal pepstatin-insensitive protease
Short name:
LPIC
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name:
TPP-I
Gene namesi
Name:Tpp1
Synonyms:Cln2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1336194 Tpp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000002737820 – 194Removed in mature formBy similarityAdd BLAST175
ChainiPRO_0000027379195 – 562Tripeptidyl-peptidase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi111 ↔ 122By similarity
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi221N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi285N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi364 ↔ 525By similarity
Glycosylationi442N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi521 ↔ 536By similarity

Post-translational modificationi

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO89023
MaxQBiO89023
PaxDbiO89023
PeptideAtlasiO89023
PRIDEiO89023

PTM databases

iPTMnetiO89023
PhosphoSitePlusiO89023
UniCarbKBiO89023

Expressioni

Gene expression databases

BgeeiENSMUSG00000030894
CleanExiMM_TPP1
ExpressionAtlasiO89023 baseline and differential
GenevisibleiO89023 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with CLN5 (PubMed:19941651).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Pot1Q91WC12EBI-7051084,EBI-7051001

Protein-protein interaction databases

BioGridi198754, 2 interactors
IntActiO89023, 5 interactors
MINTiO89023
STRINGi10090.ENSMUSP00000033184

Structurei

3D structure databases

ProteinModelPortaliO89023
SMRiO89023
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 562Peptidase S53Add BLAST365

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IICY Eukaryota
COG4934 LUCA
GeneTreeiENSGT00390000008684
HOGENOMiHOG000171253
HOVERGENiHBG004449
InParanoidiO89023
KOiK01279
OMAiISTWVYS
OrthoDBiEOG091G059I
PhylomeDBiO89023
TreeFamiTF333497

Family and domain databases

CDDicd04056 Peptidases_S53, 1 hit
cd11377 Pro-peptidase_S53, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR036852 Peptidase_S8/S53_dom_sf
IPR015366 S53_propep
IPR030400 Sedolisin_dom
PfamiView protein in Pfam
PF09286 Pro-kuma_activ, 1 hit
SMARTiView protein in SMART
SM00944 Pro-kuma_activ, 1 hit
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51695 SEDOLISIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLQARLLGL LALVIAGKCT YNPEPDQRWM LPPGWVSLGR VDPEEELSLT
60 70 80 90 100
FALKQRNLER LSELVQAVSD PSSPQYGKYL TLEDVAELVQ PSPLTLLTVQ
110 120 130 140 150
KWLSAAGARN CDSVTTQDFL TCWLSVRQAE LLLPGAEFHR YVGGPTKTHV
160 170 180 190 200
IRSPHPYQLP QALAPHVDFV GGLHRFPPSS PRQRPEPQQV GTVSLHLGVT
210 220 230 240 250
PSVLRQRYNL TAKDVGSGTT NNSQACAQFL EQYFHNSDLT EFMRLFGGSF
260 270 280 290 300
THQASVAKVV GKQGRGRAGI EASLDVEYLM SAGANISTWV YSSPGRHEAQ
310 320 330 340 350
EPFLQWLLLL SNESSLPHVH TVSYGDDEDS LSSIYIQRVN TEFMKAAARG
360 370 380 390 400
LTLLFASGDT GAGCWSVSGR HKFRPSFPAS SPYVTTVGGT SFKNPFLITD
410 420 430 440 450
EVVDYISGGG FSNVFPRPPY QEEAVAQFLK SSSHLPPSSY FNASGRAYPD
460 470 480 490 500
VAALSDGYWV VSNMVPIPWV SGTSASTPVF GGILSLINEH RILNGRPPLG
510 520 530 540 550
FLNPRLYQQH GTGLFDVTHG CHESCLNEEV EGQGFCSGPG WDPVTGWGTP
560
NFPALLKTLL NP
Length:562
Mass (Da):61,342
Last modified:December 1, 2000 - v2
Checksum:i0AF8163EA1A66396
GO

Sequence cautioni

The sequence CAA09863 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → V (PubMed:9989590).Curated1
Sequence conflicti562P → LDPFVP (PubMed:9989590).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011912 mRNA Translation: CAA09863.1 Different initiation.
AF124599 Genomic DNA Translation: AAD32573.1
AF111172 mRNA Translation: AAD03083.1
AK002418 mRNA Translation: BAB22085.1
AK048279 mRNA Translation: BAC33293.1
AK170781 mRNA Translation: BAE42026.1
BC024820 mRNA Translation: AAH24820.1
CCDSiCCDS21661.1
RefSeqiNP_034036.1, NM_009906.6
UniGeneiMm.20837

Genome annotation databases

EnsembliENSMUST00000033184; ENSMUSP00000033184; ENSMUSG00000030894
GeneIDi12751
KEGGimmu:12751
UCSCiuc009izg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTPP1_MOUSE
AccessioniPrimary (citable) accession number: O89023
Secondary accession number(s): Q543Q8, Q9QUS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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