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O89020

- AFAM_MOUSE

UniProt

O89020 - AFAM_MOUSE

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Protein
Afamin
Gene
Afm
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier By similarity.

GO - Molecular functioni

  1. vitamin E binding Source: UniProtKB

GO - Biological processi

  1. vitamin transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2429409. Afm.

Subcellular locationi

Secreted
Note: Detected in large and small blood vessels throughout the cortex and the striatum.

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 By similarity
Add
BLAST
Chaini22 – 608587Afamin
PRO_0000001107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)3 Publications
Disulfide bondi77 ↔ 86 By similarity
Disulfide bondi99 ↔ 114 By similarity
Glycosylationi109 – 1091N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi113 ↔ 124 By similarity
Disulfide bondi148 ↔ 193 By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)1 Publication
Disulfide bondi224 ↔ 270 By similarity
Disulfide bondi269 ↔ 277 By similarity
Disulfide bondi289 ↔ 303 By similarity
Disulfide bondi302 ↔ 313 By similarity
Disulfide bondi340 ↔ 385 By similarity
Disulfide bondi384 ↔ 393 By similarity
Glycosylationi402 – 4021N-linked (GlcNAc...)3 Publications
Disulfide bondi416 ↔ 462 By similarity
Disulfide bondi461 ↔ 470 By similarity
Disulfide bondi483 ↔ 499 By similarity
Glycosylationi488 – 4881N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi498 ↔ 509 By similarity
Disulfide bondi580 ↔ 589 By similarity

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO89020.
PaxDbiO89020.
PRIDEiO89020.

PTM databases

PhosphoSiteiO89020.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Expressed in isolated brain capillaries.1 Publication

Gene expression databases

BgeeiO89020.
CleanExiMM_AFM.
GenevestigatoriO89020.

Interactioni

Protein-protein interaction databases

IntActiO89020. 1 interaction.
MINTiMINT-4087327.

Structurei

3D structure databases

ProteinModelPortaliO89020.
SMRiO89020. Positions 40-603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 210189Albumin 1
Add
BLAST
Domaini211 – 403193Albumin 2
Add
BLAST
Domaini404 – 599196Albumin 3
Add
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG47806.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiO89020.
OMAiFACVDNL.
OrthoDBiEOG7DZ8JF.
PhylomeDBiO89020.
TreeFamiTF335561.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR001703. Alpha-fetoprotein.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O89020-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA    50
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD 100
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA 150
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT 200
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI 250
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK 300
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF 350
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK 400
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL 450
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK 500
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF 550
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE 600
SQATEKQR 608
Length:608
Mass (Da):69,379
Last modified:February 20, 2007 - v2
Checksum:i98B31E6D96E73F12
GO
Isoform 2 (identifier: O89020-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: H → Q
     431-608: Missing.

Show »
Length:430
Mass (Da):49,275
Checksum:i1CF6CB19386A4976
GO
Isoform 3 (identifier: O89020-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-608: KQR → NKITDQ

Note: No experimental confirmation available.

Show »
Length:611
Mass (Da):69,666
Checksum:iF0EE392CF6F91D96
GO

Sequence cautioni

The sequence AAH26681.1 differs from that shown. Reason: Contaminating sequence.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei430 – 4301H → Q in isoform 2.
VSP_023387
Alternative sequencei431 – 608178Missing in isoform 2.
VSP_023388Add
BLAST
Alternative sequencei606 – 6083KQR → NKITDQ in isoform 3.
VSP_023389

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441T → A in CAA09471. 1 Publication
Sequence conflicti44 – 441T → A in AAH26681. 1 Publication
Sequence conflicti154 – 1541N → K in CAA09471. 1 Publication
Sequence conflicti280 – 2801S → N in AAI00598. 1 Publication
Sequence conflicti285 – 2851V → E in CAA09471. 1 Publication
Sequence conflicti295 – 2951I → V in AAI00598. 1 Publication
Sequence conflicti307 – 3071T → I in AAI00598. 1 Publication
Sequence conflicti369 – 3691E → R in CAA09471. 1 Publication
Sequence conflicti417 – 4171K → N in CAA09471. 1 Publication
Sequence conflicti520 – 5201E → A in CAA09471. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011080 mRNA. Translation: CAA09471.1.
AK143987 mRNA. Translation: BAE25647.1.
BC026681 mRNA. Translation: AAH26681.1. Sequence problems.
BC100597 mRNA. Translation: AAI00598.1.
CCDSiCCDS39141.1. [O89020-1]
RefSeqiNP_660128.2. NM_145146.2. [O89020-1]
UniGeneiMm.348786.

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369. [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369. [O89020-2]
GeneIDi280662.
KEGGimmu:280662.
UCSCiuc008ybb.1. mouse. [O89020-2]
uc008ybc.1. mouse. [O89020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011080 mRNA. Translation: CAA09471.1 .
AK143987 mRNA. Translation: BAE25647.1 .
BC026681 mRNA. Translation: AAH26681.1 . Sequence problems.
BC100597 mRNA. Translation: AAI00598.1 .
CCDSi CCDS39141.1. [O89020-1 ]
RefSeqi NP_660128.2. NM_145146.2. [O89020-1 ]
UniGenei Mm.348786.

3D structure databases

ProteinModelPortali O89020.
SMRi O89020. Positions 40-603.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O89020. 1 interaction.
MINTi MINT-4087327.

PTM databases

PhosphoSitei O89020.

Proteomic databases

MaxQBi O89020.
PaxDbi O89020.
PRIDEi O89020.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000113179 ; ENSMUSP00000108804 ; ENSMUSG00000029369 . [O89020-1 ]
ENSMUST00000128740 ; ENSMUSP00000117180 ; ENSMUSG00000029369 . [O89020-2 ]
GeneIDi 280662.
KEGGi mmu:280662.
UCSCi uc008ybb.1. mouse. [O89020-2 ]
uc008ybc.1. mouse. [O89020-1 ]

Organism-specific databases

CTDi 173.
MGIi MGI:2429409. Afm.

Phylogenomic databases

eggNOGi NOG47806.
GeneTreei ENSGT00390000000113.
HOGENOMi HOG000293137.
HOVERGENi HBG004207.
InParanoidi O89020.
OMAi FACVDNL.
OrthoDBi EOG7DZ8JF.
PhylomeDBi O89020.
TreeFami TF335561.

Miscellaneous databases

ChiTaRSi AFM. mouse.
NextBioi 394069.
PROi O89020.
SOURCEi Search...

Gene expression databases

Bgeei O89020.
CleanExi MM_AFM.
Genevestigatori O89020.

Family and domain databases

InterProi IPR000264. ALB/AFP/VDB.
IPR001703. Alpha-fetoprotein.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view ]
Pfami PF00273. Serum_albumin. 3 hits.
[Graphical view ]
PIRSFi PIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSi PR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTi SM00103. ALBUMIN. 3 hits.
[Graphical view ]
SUPFAMi SSF48552. SSF48552. 3 hits.
PROSITEi PS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. van Reeth T., Gabant P., Dreze P., Szpirer J., Szpirer C.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Diaphragm.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-608 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Kidney and Liver.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-153 AND ASN-402.
    Strain: C57BL/6.
    Tissue: Plasma.
  6. "Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier."
    Kratzer I., Bernhart E., Wintersperger A., Hammer A., Waltl S., Malle E., Sperk G., Wietzorrek G., Dieplinger H., Sattler W.
    J. Neurochem. 108:707-718(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402.
    Tissue: Liver.

Entry informationi

Entry nameiAFAM_MOUSE
AccessioniPrimary (citable) accession number: O89020
Secondary accession number(s): Q3UNV0, Q497E6, Q8R0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi