Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Afamin

Gene

Afm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2429409. Afm.

Subcellular locationi

  • Secreted

  • Note: Detected in large and small blood vessels throughout the cortex and the striatum.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 608587AfaminPRO_0000001107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)3 Publications
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Glycosylationi153 – 1531N-linked (GlcNAc...)1 Publication
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402 – 4021N-linked (GlcNAc...)3 Publications
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488 – 4881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO89020.
PaxDbiO89020.
PRIDEiO89020.

PTM databases

PhosphoSiteiO89020.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Expressed in isolated brain capillaries.1 Publication

Gene expression databases

BgeeiO89020.
CleanExiMM_AFM.
GenevestigatoriO89020.

Interactioni

Protein-protein interaction databases

IntActiO89020. 1 interaction.
MINTiMINT-4087327.

Structurei

3D structure databases

ProteinModelPortaliO89020.
SMRiO89020. Positions 40-603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 210189Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 403193Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini404 – 599196Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG47806.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiO89020.
OMAiFACVDNL.
OrthoDBiEOG7DZ8JF.
PhylomeDBiO89020.
TreeFamiTF335561.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O89020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA
60 70 80 90 100
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
110 120 130 140 150
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
210 220 230 240 250
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI
260 270 280 290 300
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
310 320 330 340 350
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF
360 370 380 390 400
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
410 420 430 440 450
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
510 520 530 540 550
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF
560 570 580 590 600
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE

SQATEKQR
Length:608
Mass (Da):69,379
Last modified:February 20, 2007 - v2
Checksum:i98B31E6D96E73F12
GO
Isoform 2 (identifier: O89020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: H → Q
     431-608: Missing.

Show »
Length:430
Mass (Da):49,275
Checksum:i1CF6CB19386A4976
GO
Isoform 3 (identifier: O89020-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-608: KQR → NKITDQ

Note: No experimental confirmation available.

Show »
Length:611
Mass (Da):69,666
Checksum:iF0EE392CF6F91D96
GO

Sequence cautioni

The sequence AAH26681.1 differs from that shown.Contaminating sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441T → A in CAA09471 (Ref. 1) Curated
Sequence conflicti44 – 441T → A in AAH26681 (PubMed:15489334).Curated
Sequence conflicti154 – 1541N → K in CAA09471 (Ref. 1) Curated
Sequence conflicti280 – 2801S → N in AAI00598 (PubMed:15489334).Curated
Sequence conflicti285 – 2851V → E in CAA09471 (Ref. 1) Curated
Sequence conflicti295 – 2951I → V in AAI00598 (PubMed:15489334).Curated
Sequence conflicti307 – 3071T → I in AAI00598 (PubMed:15489334).Curated
Sequence conflicti369 – 3691E → R in CAA09471 (Ref. 1) Curated
Sequence conflicti417 – 4171K → N in CAA09471 (Ref. 1) Curated
Sequence conflicti520 – 5201E → A in CAA09471 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei430 – 4301H → Q in isoform 2. 2 PublicationsVSP_023387
Alternative sequencei431 – 608178Missing in isoform 2. 2 PublicationsVSP_023388Add
BLAST
Alternative sequencei606 – 6083KQR → NKITDQ in isoform 3. 1 PublicationVSP_023389

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA. Translation: CAA09471.1.
AK143987 mRNA. Translation: BAE25647.1.
BC026681 mRNA. Translation: AAH26681.1. Sequence problems.
BC100597 mRNA. Translation: AAI00598.1.
CCDSiCCDS39141.1. [O89020-1]
RefSeqiNP_660128.2. NM_145146.2. [O89020-1]
UniGeneiMm.348786.

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369. [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369. [O89020-2]
GeneIDi280662.
KEGGimmu:280662.
UCSCiuc008ybb.1. mouse. [O89020-2]
uc008ybc.1. mouse. [O89020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA. Translation: CAA09471.1.
AK143987 mRNA. Translation: BAE25647.1.
BC026681 mRNA. Translation: AAH26681.1. Sequence problems.
BC100597 mRNA. Translation: AAI00598.1.
CCDSiCCDS39141.1. [O89020-1]
RefSeqiNP_660128.2. NM_145146.2. [O89020-1]
UniGeneiMm.348786.

3D structure databases

ProteinModelPortaliO89020.
SMRiO89020. Positions 40-603.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO89020. 1 interaction.
MINTiMINT-4087327.

PTM databases

PhosphoSiteiO89020.

Proteomic databases

MaxQBiO89020.
PaxDbiO89020.
PRIDEiO89020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369. [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369. [O89020-2]
GeneIDi280662.
KEGGimmu:280662.
UCSCiuc008ybb.1. mouse. [O89020-2]
uc008ybc.1. mouse. [O89020-1]

Organism-specific databases

CTDi173.
MGIiMGI:2429409. Afm.

Phylogenomic databases

eggNOGiNOG47806.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiO89020.
OMAiFACVDNL.
OrthoDBiEOG7DZ8JF.
PhylomeDBiO89020.
TreeFamiTF335561.

Miscellaneous databases

ChiTaRSiAfm. mouse.
NextBioi394069.
PROiO89020.
SOURCEiSearch...

Gene expression databases

BgeeiO89020.
CleanExiMM_AFM.
GenevestigatoriO89020.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. van Reeth T., Gabant P., Dreze P., Szpirer J., Szpirer C.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Diaphragm.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-608 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Kidney and Liver.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-153 AND ASN-402.
    Strain: C57BL/6.
    Tissue: Plasma.
  6. "Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier."
    Kratzer I., Bernhart E., Wintersperger A., Hammer A., Waltl S., Malle E., Sperk G., Wietzorrek G., Dieplinger H., Sattler W.
    J. Neurochem. 108:707-718(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402.
    Tissue: Liver.

Entry informationi

Entry nameiAFAM_MOUSE
AccessioniPrimary (citable) accession number: O89020
Secondary accession number(s): Q3UNV0, Q497E6, Q8R0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 20, 2007
Last modified: May 27, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.