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Protein

Afamin

Gene

Afm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2429409. Afm.

Subcellular locationi

  • Secreted

  • Note: Detected in large and small blood vessels throughout the cortex and the striatum.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000000110722 – 608AfaminAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)3 Publications1
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Glycosylationi153N-linked (GlcNAc...)1 Publication1
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402N-linked (GlcNAc...)3 Publications1
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO89020.
PaxDbiO89020.
PeptideAtlasiO89020.
PRIDEiO89020.

PTM databases

iPTMnetiO89020.
PhosphoSitePlusiO89020.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Expressed in isolated brain capillaries.1 Publication

Gene expression databases

BgeeiENSMUSG00000029369.
CleanExiMM_AFM.
GenevisibleiO89020. MM.

Interactioni

Protein-protein interaction databases

IntActiO89020. 1 interactor.
MINTiMINT-4087327.
STRINGi10090.ENSMUSP00000108804.

Structurei

3D structure databases

ProteinModelPortaliO89020.
SMRiO89020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 210Albumin 1PROSITE-ProRule annotationAdd BLAST189
Domaini211 – 403Albumin 2PROSITE-ProRule annotationAdd BLAST193
Domaini404 – 599Albumin 3PROSITE-ProRule annotationAdd BLAST196

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ. Eukaryota.
ENOG410Z40H. LUCA.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiO89020.
OMAiLDPEEKC.
OrthoDBiEOG091G0F5F.
PhylomeDBiO89020.
TreeFamiTF335561.

Family and domain databases

CDDicd00015. ALBUMIN. 3 hits.
InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O89020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA
60 70 80 90 100
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
110 120 130 140 150
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
210 220 230 240 250
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI
260 270 280 290 300
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
310 320 330 340 350
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF
360 370 380 390 400
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
410 420 430 440 450
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
510 520 530 540 550
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF
560 570 580 590 600
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE

SQATEKQR
Length:608
Mass (Da):69,379
Last modified:February 20, 2007 - v2
Checksum:i98B31E6D96E73F12
GO
Isoform 2 (identifier: O89020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: H → Q
     431-608: Missing.

Show »
Length:430
Mass (Da):49,275
Checksum:i1CF6CB19386A4976
GO
Isoform 3 (identifier: O89020-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-608: KQR → NKITDQ

Note: No experimental confirmation available.
Show »
Length:611
Mass (Da):69,666
Checksum:iF0EE392CF6F91D96
GO

Sequence cautioni

The sequence AAH26681 differs from that shown. Contaminating sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44T → A in CAA09471 (Ref. 1) Curated1
Sequence conflicti44T → A in AAH26681 (PubMed:15489334).Curated1
Sequence conflicti154N → K in CAA09471 (Ref. 1) Curated1
Sequence conflicti280S → N in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti285V → E in CAA09471 (Ref. 1) Curated1
Sequence conflicti295I → V in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti307T → I in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti369E → R in CAA09471 (Ref. 1) Curated1
Sequence conflicti417K → N in CAA09471 (Ref. 1) Curated1
Sequence conflicti520E → A in CAA09471 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023387430H → Q in isoform 2. 2 Publications1
Alternative sequenceiVSP_023388431 – 608Missing in isoform 2. 2 PublicationsAdd BLAST178
Alternative sequenceiVSP_023389606 – 608KQR → NKITDQ in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA. Translation: CAA09471.1.
AK143987 mRNA. Translation: BAE25647.1.
BC026681 mRNA. Translation: AAH26681.1. Sequence problems.
BC100597 mRNA. Translation: AAI00598.1.
CCDSiCCDS39141.1. [O89020-1]
RefSeqiNP_660128.2. NM_145146.2. [O89020-1]
UniGeneiMm.348786.

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369. [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369. [O89020-2]
GeneIDi280662.
KEGGimmu:280662.
UCSCiuc008ybb.1. mouse. [O89020-2]
uc008ybc.1. mouse. [O89020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA. Translation: CAA09471.1.
AK143987 mRNA. Translation: BAE25647.1.
BC026681 mRNA. Translation: AAH26681.1. Sequence problems.
BC100597 mRNA. Translation: AAI00598.1.
CCDSiCCDS39141.1. [O89020-1]
RefSeqiNP_660128.2. NM_145146.2. [O89020-1]
UniGeneiMm.348786.

3D structure databases

ProteinModelPortaliO89020.
SMRiO89020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO89020. 1 interactor.
MINTiMINT-4087327.
STRINGi10090.ENSMUSP00000108804.

PTM databases

iPTMnetiO89020.
PhosphoSitePlusiO89020.

Proteomic databases

MaxQBiO89020.
PaxDbiO89020.
PeptideAtlasiO89020.
PRIDEiO89020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369. [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369. [O89020-2]
GeneIDi280662.
KEGGimmu:280662.
UCSCiuc008ybb.1. mouse. [O89020-2]
uc008ybc.1. mouse. [O89020-1]

Organism-specific databases

CTDi173.
MGIiMGI:2429409. Afm.

Phylogenomic databases

eggNOGiENOG410IIRZ. Eukaryota.
ENOG410Z40H. LUCA.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiO89020.
OMAiLDPEEKC.
OrthoDBiEOG091G0F5F.
PhylomeDBiO89020.
TreeFamiTF335561.

Miscellaneous databases

ChiTaRSiAfm. mouse.
PROiO89020.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029369.
CleanExiMM_AFM.
GenevisibleiO89020. MM.

Family and domain databases

CDDicd00015. ALBUMIN. 3 hits.
InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 2 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAFAM_MOUSE
AccessioniPrimary (citable) accession number: O89020
Secondary accession number(s): Q3UNV0, Q497E6, Q8R0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.