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Protein

Carboxypeptidase D

Gene

Cpd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Releases C-terminal Arg and Lys from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Zinc 1; catalyticBy similarity1
Metal bindingi141Zinc 1; catalyticBy similarity1
Metal bindingi256Zinc 1; catalyticBy similarity1
Sitei349Important for catalytic activityBy similarity1
Metal bindingi563Zinc 2; catalyticBy similarity1
Metal bindingi566Zinc 2; catalyticBy similarity1
Metal bindingi670Zinc 2; catalyticBy similarity1
Active sitei761Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.22. 3474.
ReactomeiR-MMU-432722. Golgi Associated Vesicle Biogenesis.

Protein family/group databases

MEROPSiM14.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase D (EC:3.4.17.22)
Alternative name(s):
Metallocarboxypeptidase D
gp180
Gene namesi
Name:Cpd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107265. Cpd.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 1296ExtracellularSequence analysisAdd BLAST1259
Transmembranei1297 – 1317HelicalSequence analysisAdd BLAST21
Topological domaini1318 – 1377CytoplasmicSequence analysisAdd BLAST60

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000000440238 – 1377Carboxypeptidase DAdd BLAST1340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi216N-linked (GlcNAc...)Sequence analysis1
Modified residuei264PhosphotyrosineCombined sources1
Modified residuei269PhosphoserineCombined sources1
Glycosylationi398N-linked (GlcNAc...)1 Publication1
Glycosylationi409N-linked (GlcNAc...)1 Publication1
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1
Glycosylationi521N-linked (GlcNAc...)1 Publication1
Glycosylationi625N-linked (GlcNAc...)Sequence analysis1
Glycosylationi810N-linked (GlcNAc...)Sequence analysis1
Glycosylationi854N-linked (GlcNAc...)Sequence analysis1
Glycosylationi866N-linked (GlcNAc...)Sequence analysis1
Glycosylationi878N-linked (GlcNAc...)Sequence analysis1
Glycosylationi952N-linked (GlcNAc...)Sequence analysis1
Glycosylationi975N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1067N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1139N-linked (GlcNAc...)Sequence analysis1
Lipidationi1314S-palmitoyl cysteineBy similarity1
Lipidationi1318S-palmitoyl cysteineBy similarity1
Lipidationi1320S-palmitoyl cysteineBy similarity1
Modified residuei1355PhosphoserineCombined sources1
Modified residuei1358PhosphoserineCombined sources1
Modified residuei1365PhosphothreonineCombined sources1
Modified residuei1367PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiO89001.
PaxDbiO89001.
PeptideAtlasiO89001.
PRIDEiO89001.

PTM databases

iPTMnetiO89001.
PhosphoSitePlusiO89001.
SwissPalmiO89001.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020841.
CleanExiMM_CPD.
GenevisibleiO89001. MM.

Interactioni

Protein-protein interaction databases

IntActiO89001. 3 interactors.
MINTiMINT-4996296.
STRINGi10090.ENSMUSP00000021201.

Structurei

3D structure databases

ProteinModelPortaliO89001.
SMRiO89001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 492Carboxypeptidase-like 1Add BLAST455
Regioni493 – 896Carboxypeptidase-like 2Add BLAST404
Regioni897 – 1296Carboxypeptidase-like 3Add BLAST400

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi161 – 163Cell attachment siteSequence analysis3

Domaini

There are 3 carboxypeptidase-like domains. Only the first two domains seem to have kept a catalytic activity.

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000046445.
HOVERGENiHBG006932.
InParanoidiO89001.
KOiK07752.
OMAiDWIRRNK.
OrthoDBiEOG091G06A9.
TreeFamiTF315592.

Family and domain databases

CDDicd06245. M14_CPD_III. 1 hit.
Gene3Di2.60.40.1120. 3 hits.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR033848. Carboxypeptidase_D_III.
IPR015567. Pept_M14B_carboxypept_D2.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF50. PTHR11532:SF50. 3 hits.
PfamiPF00246. Peptidase_M14. 3 hits.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 3 hits.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 3 hits.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 2 hits.
PS00133. CARBOXYPEPT_ZN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGRDERPP WRLGRLRLLP PPPLLLLLLL LRSSAQAAHI KKAEATTTTV
60 70 80 90 100
GGSEAAEGQF DHYYHEAALG EALEAAAAAG PPGLARLFSI GSSVEGRPLW
110 120 130 140 150
VLRLTAGLGP PPTAAAGLDA AGPLLPGRPQ VKLVGNMHGD ETVSRQVLVY
160 170 180 190 200
LARELASGYR RGDPRLVRLL NTTDVYLLPS LNPDGFERAR EGDCGLGDSG
210 220 230 240 250
PPGTSGRDNS RGRDLNRSFP DQFSTGEPPS LDEVPEVRAL IDWIRRNKFV
260 270 280 290 300
LSGNLHGGSV VASYPFDDSP EHKTTGLYSK TSDDEVFRYL AKAYASNHPI
310 320 330 340 350
MKTGEPHCPG DEDETFKDGI TNGAHWYDVE GGMQDYNYVW ANCFEITLEL
360 370 380 390 400
SCCKYPPASQ LRQEWENNRE SLITLIEKVH IGIKGFVKDS VTGSGLENAT
410 420 430 440 450
ISVAGINHNI TTGRFGDFHR LLVPGTYNLT ALSTGYMPLT INNIMVKEGP
460 470 480 490 500
ATEMDFSLRP TVMSVMPGST EAVTTPGTVA VPNIPPGTPS SHQPIQPKDF
510 520 530 540 550
HHHHFPDMEI FLRRFANEYP NITRLYSLGK SVESRELYVM EISDNPGVHE
560 570 580 590 600
PGEPEFKYIG NMHGNEVVGR ELLLNLIEYL CKNFGTDPEV TDLVRSTRIH
610 620 630 640 650
LMPSMNPDGY EKSQEGDSIS VVGRNNSNNF DLNRNFPDQF VPITEPTQPE
660 670 680 690 700
TIAVMSWVKA YPFVLSANLH GGSLVVNYPY DDNEQGVATY SKSPDDAVFQ
710 720 730 740 750
QIALSYSKEN SQMFQGRPCK DMYLNEYFPH GITNGASWYN VPGGMQDWNY
760 770 780 790 800
LQTNCFEVTI ELGCVKYPFE NELPKYWEQN RRSLIQFMKQ VHQGVKGFVL
810 820 830 840 850
DATDGRGILN ATLSVAEINH PVTTYKAGDY WRLLVPGTYK ITASARGYNP
860 870 880 890 900
VTKNVTVRSE GAVQVNFTLV RSSADANNES KKGRGHSTST DDTSDPTSKE
910 920 930 940 950
FEALIKHLSA ENGLEGFMLS SSSDLALYRY HSYKDLSEFL RGLVMNYPHI
960 970 980 990 1000
TNLTTLGQSV EYRHIWSLEI SNKPNISEPE EPKIRFVAGI HGNAPVGTEL
1010 1020 1030 1040 1050
LLALAEFLCL NYKRNPVVTQ LVDRTRIVIV PSLNPDGRER AQEKDCTSKT
1060 1070 1080 1090 1100
GHTNAHGKDL DTDFTSNASQ PETKAIIENL IQKQDFSLSI ALDGGSVLVT
1110 1120 1130 1140 1150
YPYDKPVQTV ENKETLKHLA SLYANNHPSM HMGQPSCPNN SDENIPGGVM
1160 1170 1180 1190 1200
RGAEWHSHLG SMKDYSVTYG HCPEITVYTS CCYFPSAAQL PALWAENKKS
1210 1220 1230 1240 1250
LLSMLVEVHK GVHGLVKDKA GKPISKAVIV LNEGIKVYTK EGGYFHVLLA
1260 1270 1280 1290 1300
PGVHNINAIA DGYQQQHTQV FVHHDAASSV VIVFDTDNRI FGLPRELVVT
1310 1320 1330 1340 1350
VSGATMSALI LTACIIWCIC SIKSNRHKDG FHRLRQHHDE YEDEIRMMST
1360 1370
GSKKSLLSHE FQDETDTEEE TLYSSKH
Length:1,377
Mass (Da):152,406
Last modified:July 27, 2011 - v2
Checksum:i73CC0861EE674053
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53S → T in BAA33371 (PubMed:9714835).Curated1
Sequence conflicti1039 – 1040ER → DG in BAA33371 (PubMed:9714835).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85391 mRNA. Translation: BAA33371.1.
AK137737 mRNA. Translation: BAE23483.1.
AL645479 Genomic DNA. Translation: CAI25538.1.
CCDSiCCDS25071.1.
RefSeqiNP_031780.2. NM_007754.2.
UniGeneiMm.276736.

Genome annotation databases

EnsembliENSMUST00000021201; ENSMUSP00000021201; ENSMUSG00000020841.
GeneIDi12874.
KEGGimmu:12874.
UCSCiuc007kga.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85391 mRNA. Translation: BAA33371.1.
AK137737 mRNA. Translation: BAE23483.1.
AL645479 Genomic DNA. Translation: CAI25538.1.
CCDSiCCDS25071.1.
RefSeqiNP_031780.2. NM_007754.2.
UniGeneiMm.276736.

3D structure databases

ProteinModelPortaliO89001.
SMRiO89001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO89001. 3 interactors.
MINTiMINT-4996296.
STRINGi10090.ENSMUSP00000021201.

Protein family/group databases

MEROPSiM14.011.

PTM databases

iPTMnetiO89001.
PhosphoSitePlusiO89001.
SwissPalmiO89001.

Proteomic databases

MaxQBiO89001.
PaxDbiO89001.
PeptideAtlasiO89001.
PRIDEiO89001.

Protocols and materials databases

DNASUi12874.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021201; ENSMUSP00000021201; ENSMUSG00000020841.
GeneIDi12874.
KEGGimmu:12874.
UCSCiuc007kga.1. mouse.

Organism-specific databases

CTDi1362.
MGIiMGI:107265. Cpd.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000046445.
HOVERGENiHBG006932.
InParanoidiO89001.
KOiK07752.
OMAiDWIRRNK.
OrthoDBiEOG091G06A9.
TreeFamiTF315592.

Enzyme and pathway databases

BRENDAi3.4.17.22. 3474.
ReactomeiR-MMU-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiCpd. mouse.
PROiO89001.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020841.
CleanExiMM_CPD.
GenevisibleiO89001. MM.

Family and domain databases

CDDicd06245. M14_CPD_III. 1 hit.
Gene3Di2.60.40.1120. 3 hits.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR033848. Carboxypeptidase_D_III.
IPR015567. Pept_M14B_carboxypept_D2.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF50. PTHR11532:SF50. 3 hits.
PfamiPF00246. Peptidase_M14. 3 hits.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 3 hits.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 3 hits.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 2 hits.
PS00133. CARBOXYPEPT_ZN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPD_MOUSE
AccessioniPrimary (citable) accession number: O89001
Secondary accession number(s): Q5SVH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.