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O88990

- ACTN3_MOUSE

UniProt

O88990 - ACTN3_MOUSE

Protein

Alpha-actinin-3

Gene

Actn3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi772 – 783121; possibly ancestralAdd
    BLAST
    Calcium bindingi808 – 819122; possibly ancestralAdd
    BLAST

    GO - Molecular functioni

    1. actin filament binding Source: MGI
    2. calcium ion binding Source: InterPro
    3. protein binding Source: MGI
    4. protein binding, bridging Source: MGI
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. muscle contraction Source: MGI

    Keywords - Ligandi

    Actin-binding, Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-actinin-3
    Alternative name(s):
    Alpha-actinin skeletal muscle isoform 3
    F-actin cross-linking protein
    Gene namesi
    Name:Actn3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:99678. Actn3.

    Subcellular locationi

    GO - Cellular componenti

    1. sarcomere Source: MGI
    2. striated muscle thin filament Source: MGI
    3. Z disc Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Alpha-actinin-3PRO_0000073439Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO88990.
    PaxDbiO88990.
    PRIDEiO88990.

    PTM databases

    PhosphoSiteiO88990.

    Expressioni

    Gene expression databases

    BgeeiO88990.
    CleanExiMM_ACTN3.
    GenevestigatoriO88990.

    Interactioni

    Subunit structurei

    Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi197951. 2 interactions.
    IntActiO88990. 4 interactions.
    MINTiMINT-4086780.

    Structurei

    3D structure databases

    ProteinModelPortaliO88990.
    SMRiO88990. Positions 41-267, 280-900.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 260260Actin-bindingAdd
    BLAST
    Domaini44 – 148105CH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini157 – 260104CH 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati287 – 397111Spectrin 1Add
    BLAST
    Repeati407 – 512106Spectrin 2Add
    BLAST
    Repeati522 – 633112Spectrin 3Add
    BLAST
    Repeati643 – 746104Spectrin 4Add
    BLAST
    Domaini759 – 79436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini795 – 83036EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alpha-actinin family.Curated
    Contains 1 actin-binding domain.Curated
    Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 4 spectrin repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5069.
    GeneTreeiENSGT00670000097825.
    HOGENOMiHOG000263418.
    HOVERGENiHBG050453.
    InParanoidiQ14DS8.
    KOiK05699.
    OMAiGPWIQGK.
    OrthoDBiEOG72C4ZJ.
    PhylomeDBiO88990.
    TreeFamiTF352676.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProiIPR001589. Actinin_actin-bd_CS.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view]
    PfamiPF00307. CH. 2 hits.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view]
    SMARTiSM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 2 hits.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    PROSITEiPS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88990-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMMVMQPEGL GAGEGPFSGG GGGEYMEQEE DWDRDLLLDP AWEKQQRKTF    50
    TAWCNSHLRK AGTQIENIEE DFRNGLKLML LLEVISGERL PRPDKGKMRF 100
    HKIANVNKAL DFIASKGVKL VSIGAEEIVD GNLKMTLGMI WTIILRFAIQ 150
    DISVEETSAK EGLLLWCQRK TAPYRNVNVQ NFHTSWKDGL ALCALIHRHR 200
    PDLIDYAKLR KDDPIGNLNT AFEVAEKYLD IPKMLDAEDI VNTPKPDEKA 250
    IMTYVSCFYH AFAGAEQAET AANRICKVLA VNQENEKLME EYEKLASELL 300
    EWIRRTVPWL ENRVGEPSMS AMQRKLEDFR DYRRLHKPPR VQEKCQLEIN 350
    FNTLQTKLRL SHRPAFMPSE GKLVSDIANA WRGLEQVEKG YEDWLLSEIR 400
    RLQRLQHLAE KFQQKASLHE AWTRGKEEML NQHDYESASL QEVRALLRRH 450
    EAFESDLAAH QDRVEHIAAL AQELNELDYH EAASVNSRCQ AICDQWDNLG 500
    TLTQKRRDAL ERMEKLLETI DQLQLEFARR AAPFNNWLDG AIEDLQDVWL 550
    VHSVEETQSL LTAHEQFKAT LPEADRERGA ILGIQGEIQK ICQTYGLRPK 600
    SGNPYITLSS QDINNKWDTV RKLVPSRDQT LQEELARQQV NERLRRQFAA 650
    QANAIGPWIQ GKVEEVGRLA AGLAGSLEEQ MAGLRQQEQN IINYKSNIDR 700
    LEGDHQLLQE SLVFDNKHTV YSMEHIRVGW EQLLTSIART INEVENQVLT 750
    RDAKGLSQEQ LNEFRASFNH FDRKRNGMME PDDFRACLIS MGYDLGEVEF 800
    ARIMTMVDPN AAGVVTFQAF IDFMTRETAE TDTAEQVVAS FKILAGDKNY 850
    ITPEELRREL PAEQAEYCIR RMAPYKGSGA PSGALDYVAF SSALYGESDL 900
    Length:900
    Mass (Da):103,043
    Last modified:November 1, 1998 - v1
    Checksum:i7F795002CB2B7F8B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093775 mRNA. Translation: AAC62512.1.
    BC111890 mRNA. Translation: AAI11891.1.
    CCDSiCCDS29441.1.
    RefSeqiNP_038484.1. NM_013456.2.
    UniGeneiMm.5316.

    Genome annotation databases

    EnsembliENSMUST00000006626; ENSMUSP00000006626; ENSMUSG00000006457.
    GeneIDi11474.
    KEGGimmu:11474.
    UCSCiuc008gbf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093775 mRNA. Translation: AAC62512.1 .
    BC111890 mRNA. Translation: AAI11891.1 .
    CCDSi CCDS29441.1.
    RefSeqi NP_038484.1. NM_013456.2.
    UniGenei Mm.5316.

    3D structure databases

    ProteinModelPortali O88990.
    SMRi O88990. Positions 41-267, 280-900.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 197951. 2 interactions.
    IntActi O88990. 4 interactions.
    MINTi MINT-4086780.

    PTM databases

    PhosphoSitei O88990.

    Proteomic databases

    MaxQBi O88990.
    PaxDbi O88990.
    PRIDEi O88990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006626 ; ENSMUSP00000006626 ; ENSMUSG00000006457 .
    GeneIDi 11474.
    KEGGi mmu:11474.
    UCSCi uc008gbf.1. mouse.

    Organism-specific databases

    CTDi 89.
    MGIi MGI:99678. Actn3.

    Phylogenomic databases

    eggNOGi COG5069.
    GeneTreei ENSGT00670000097825.
    HOGENOMi HOG000263418.
    HOVERGENi HBG050453.
    InParanoidi Q14DS8.
    KOi K05699.
    OMAi GPWIQGK.
    OrthoDBi EOG72C4ZJ.
    PhylomeDBi O88990.
    TreeFami TF352676.

    Miscellaneous databases

    NextBioi 278812.
    PROi O88990.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88990.
    CleanExi MM_ACTN3.
    Genevestigatori O88990.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProi IPR001589. Actinin_actin-bd_CS.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view ]
    Pfami PF00307. CH. 2 hits.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view ]
    SMARTi SM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    PROSITEi PS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Birkenmeier C.S., Gifford E.J., Barker J.E.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Skeletal muscle.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiACTN3_MOUSE
    AccessioniPrimary (citable) accession number: O88990
    Secondary accession number(s): Q14DS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3