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O88990

- ACTN3_MOUSE

UniProt

O88990 - ACTN3_MOUSE

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Protein
Alpha-actinin-3
Gene
Actn3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi772 – 783121; possibly ancestral
Add
BLAST
Calcium bindingi808 – 819122; possibly ancestral
Add
BLAST

GO - Molecular functioni

  1. actin filament binding Source: MGI
  2. calcium ion binding Source: InterPro
  3. protein binding Source: MGI
  4. protein binding, bridging Source: MGI
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. muscle contraction Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-3
Alternative name(s):
Alpha-actinin skeletal muscle isoform 3
F-actin cross-linking protein
Gene namesi
Name:Actn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:99678. Actn3.

Subcellular locationi

GO - Cellular componenti

  1. Z disc Source: MGI
  2. sarcomere Source: MGI
  3. striated muscle thin filament Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Alpha-actinin-3
PRO_0000073439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO88990.
PaxDbiO88990.
PRIDEiO88990.

PTM databases

PhosphoSiteiO88990.

Expressioni

Gene expression databases

BgeeiO88990.
CleanExiMM_ACTN3.
GenevestigatoriO88990.

Interactioni

Subunit structurei

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1 By similarity.

Protein-protein interaction databases

BioGridi197951. 2 interactions.
IntActiO88990. 4 interactions.
MINTiMINT-4086780.

Structurei

3D structure databases

ProteinModelPortaliO88990.
SMRiO88990. Positions 41-267, 280-900.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 260260Actin-binding
Add
BLAST
Domaini44 – 148105CH 1
Add
BLAST
Domaini157 – 260104CH 2
Add
BLAST
Repeati287 – 397111Spectrin 1
Add
BLAST
Repeati407 – 512106Spectrin 2
Add
BLAST
Repeati522 – 633112Spectrin 3
Add
BLAST
Repeati643 – 746104Spectrin 4
Add
BLAST
Domaini759 – 79436EF-hand 1
Add
BLAST
Domaini795 – 83036EF-hand 2
Add
BLAST

Sequence similaritiesi

Belongs to the alpha-actinin family.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5069.
GeneTreeiENSGT00670000097825.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiQ14DS8.
KOiK05699.
OMAiGPWIQGK.
OrthoDBiEOG72C4ZJ.
PhylomeDBiO88990.
TreeFamiTF352676.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88990-1 [UniParc]FASTAAdd to Basket

« Hide

MMMVMQPEGL GAGEGPFSGG GGGEYMEQEE DWDRDLLLDP AWEKQQRKTF    50
TAWCNSHLRK AGTQIENIEE DFRNGLKLML LLEVISGERL PRPDKGKMRF 100
HKIANVNKAL DFIASKGVKL VSIGAEEIVD GNLKMTLGMI WTIILRFAIQ 150
DISVEETSAK EGLLLWCQRK TAPYRNVNVQ NFHTSWKDGL ALCALIHRHR 200
PDLIDYAKLR KDDPIGNLNT AFEVAEKYLD IPKMLDAEDI VNTPKPDEKA 250
IMTYVSCFYH AFAGAEQAET AANRICKVLA VNQENEKLME EYEKLASELL 300
EWIRRTVPWL ENRVGEPSMS AMQRKLEDFR DYRRLHKPPR VQEKCQLEIN 350
FNTLQTKLRL SHRPAFMPSE GKLVSDIANA WRGLEQVEKG YEDWLLSEIR 400
RLQRLQHLAE KFQQKASLHE AWTRGKEEML NQHDYESASL QEVRALLRRH 450
EAFESDLAAH QDRVEHIAAL AQELNELDYH EAASVNSRCQ AICDQWDNLG 500
TLTQKRRDAL ERMEKLLETI DQLQLEFARR AAPFNNWLDG AIEDLQDVWL 550
VHSVEETQSL LTAHEQFKAT LPEADRERGA ILGIQGEIQK ICQTYGLRPK 600
SGNPYITLSS QDINNKWDTV RKLVPSRDQT LQEELARQQV NERLRRQFAA 650
QANAIGPWIQ GKVEEVGRLA AGLAGSLEEQ MAGLRQQEQN IINYKSNIDR 700
LEGDHQLLQE SLVFDNKHTV YSMEHIRVGW EQLLTSIART INEVENQVLT 750
RDAKGLSQEQ LNEFRASFNH FDRKRNGMME PDDFRACLIS MGYDLGEVEF 800
ARIMTMVDPN AAGVVTFQAF IDFMTRETAE TDTAEQVVAS FKILAGDKNY 850
ITPEELRREL PAEQAEYCIR RMAPYKGSGA PSGALDYVAF SSALYGESDL 900
Length:900
Mass (Da):103,043
Last modified:November 1, 1998 - v1
Checksum:i7F795002CB2B7F8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093775 mRNA. Translation: AAC62512.1.
BC111890 mRNA. Translation: AAI11891.1.
CCDSiCCDS29441.1.
RefSeqiNP_038484.1. NM_013456.2.
UniGeneiMm.5316.

Genome annotation databases

EnsembliENSMUST00000006626; ENSMUSP00000006626; ENSMUSG00000006457.
GeneIDi11474.
KEGGimmu:11474.
UCSCiuc008gbf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093775 mRNA. Translation: AAC62512.1 .
BC111890 mRNA. Translation: AAI11891.1 .
CCDSi CCDS29441.1.
RefSeqi NP_038484.1. NM_013456.2.
UniGenei Mm.5316.

3D structure databases

ProteinModelPortali O88990.
SMRi O88990. Positions 41-267, 280-900.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 197951. 2 interactions.
IntActi O88990. 4 interactions.
MINTi MINT-4086780.

PTM databases

PhosphoSitei O88990.

Proteomic databases

MaxQBi O88990.
PaxDbi O88990.
PRIDEi O88990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006626 ; ENSMUSP00000006626 ; ENSMUSG00000006457 .
GeneIDi 11474.
KEGGi mmu:11474.
UCSCi uc008gbf.1. mouse.

Organism-specific databases

CTDi 89.
MGIi MGI:99678. Actn3.

Phylogenomic databases

eggNOGi COG5069.
GeneTreei ENSGT00670000097825.
HOGENOMi HOG000263418.
HOVERGENi HBG050453.
InParanoidi Q14DS8.
KOi K05699.
OMAi GPWIQGK.
OrthoDBi EOG72C4ZJ.
PhylomeDBi O88990.
TreeFami TF352676.

Miscellaneous databases

NextBioi 278812.
PROi O88990.
SOURCEi Search...

Gene expression databases

Bgeei O88990.
CleanExi MM_ACTN3.
Genevestigatori O88990.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProi IPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view ]
Pfami PF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view ]
SMARTi SM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
[Graphical view ]
SUPFAMi SSF47576. SSF47576. 1 hit.
PROSITEi PS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Birkenmeier C.S., Gifford E.J., Barker J.E.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiACTN3_MOUSE
AccessioniPrimary (citable) accession number: O88990
Secondary accession number(s): Q14DS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi