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O88990 (ACTN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-actinin-3
Alternative name(s):
Alpha-actinin skeletal muscle isoform 3
F-actin cross-linking protein
Gene names
Name:Actn3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length900 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.

Subunit structure

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1 By similarity.

Sequence similarities

Belongs to the alpha-actinin family.

Contains 1 actin-binding domain.

Contains 2 CH (calponin-homology) domains.

Contains 2 EF-hand domains.

Contains 4 spectrin repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 900900Alpha-actinin-3
PRO_0000073439

Regions

Domain1 – 260260Actin-binding
Domain44 – 148105CH 1
Domain157 – 260104CH 2
Repeat287 – 397111Spectrin 1
Repeat407 – 512106Spectrin 2
Repeat522 – 633112Spectrin 3
Repeat643 – 746104Spectrin 4
Domain759 – 79436EF-hand 1
Domain795 – 83036EF-hand 2
Calcium binding772 – 783121; possibly ancestral
Calcium binding808 – 819122; possibly ancestral

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
O88990 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7F795002CB2B7F8B

FASTA900103,043
        10         20         30         40         50         60 
MMMVMQPEGL GAGEGPFSGG GGGEYMEQEE DWDRDLLLDP AWEKQQRKTF TAWCNSHLRK 

        70         80         90        100        110        120 
AGTQIENIEE DFRNGLKLML LLEVISGERL PRPDKGKMRF HKIANVNKAL DFIASKGVKL 

       130        140        150        160        170        180 
VSIGAEEIVD GNLKMTLGMI WTIILRFAIQ DISVEETSAK EGLLLWCQRK TAPYRNVNVQ 

       190        200        210        220        230        240 
NFHTSWKDGL ALCALIHRHR PDLIDYAKLR KDDPIGNLNT AFEVAEKYLD IPKMLDAEDI 

       250        260        270        280        290        300 
VNTPKPDEKA IMTYVSCFYH AFAGAEQAET AANRICKVLA VNQENEKLME EYEKLASELL 

       310        320        330        340        350        360 
EWIRRTVPWL ENRVGEPSMS AMQRKLEDFR DYRRLHKPPR VQEKCQLEIN FNTLQTKLRL 

       370        380        390        400        410        420 
SHRPAFMPSE GKLVSDIANA WRGLEQVEKG YEDWLLSEIR RLQRLQHLAE KFQQKASLHE 

       430        440        450        460        470        480 
AWTRGKEEML NQHDYESASL QEVRALLRRH EAFESDLAAH QDRVEHIAAL AQELNELDYH 

       490        500        510        520        530        540 
EAASVNSRCQ AICDQWDNLG TLTQKRRDAL ERMEKLLETI DQLQLEFARR AAPFNNWLDG 

       550        560        570        580        590        600 
AIEDLQDVWL VHSVEETQSL LTAHEQFKAT LPEADRERGA ILGIQGEIQK ICQTYGLRPK 

       610        620        630        640        650        660 
SGNPYITLSS QDINNKWDTV RKLVPSRDQT LQEELARQQV NERLRRQFAA QANAIGPWIQ 

       670        680        690        700        710        720 
GKVEEVGRLA AGLAGSLEEQ MAGLRQQEQN IINYKSNIDR LEGDHQLLQE SLVFDNKHTV 

       730        740        750        760        770        780 
YSMEHIRVGW EQLLTSIART INEVENQVLT RDAKGLSQEQ LNEFRASFNH FDRKRNGMME 

       790        800        810        820        830        840 
PDDFRACLIS MGYDLGEVEF ARIMTMVDPN AAGVVTFQAF IDFMTRETAE TDTAEQVVAS 

       850        860        870        880        890        900 
FKILAGDKNY ITPEELRREL PAEQAEYCIR RMAPYKGSGA PSGALDYVAF SSALYGESDL 

« Hide

References

« Hide 'large scale' references
[1]Birkenmeier C.S., Gifford E.J., Barker J.E.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Skeletal muscle.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093775 mRNA. Translation: AAC62512.1.
BC111890 mRNA. Translation: AAI11891.1.
CCDSCCDS29441.1.
RefSeqNP_038484.1. NM_013456.2.
UniGeneMm.5316.

3D structure databases

ProteinModelPortalO88990.
SMRO88990. Positions 41-267, 280-900.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid197951. 2 interactions.
IntActO88990. 4 interactions.
MINTMINT-4086780.

PTM databases

PhosphoSiteO88990.

Proteomic databases

MaxQBO88990.
PaxDbO88990.
PRIDEO88990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006626; ENSMUSP00000006626; ENSMUSG00000006457.
GeneID11474.
KEGGmmu:11474.
UCSCuc008gbf.1. mouse.

Organism-specific databases

CTD89.
MGIMGI:99678. Actn3.

Phylogenomic databases

eggNOGCOG5069.
GeneTreeENSGT00670000097825.
HOGENOMHOG000263418.
HOVERGENHBG050453.
InParanoidQ14DS8.
KOK05699.
OMAGPWIQGK.
OrthoDBEOG72C4ZJ.
PhylomeDBO88990.
TreeFamTF352676.

Gene expression databases

BgeeO88990.
CleanExMM_ACTN3.
GenevestigatorO88990.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
PROSITEPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio278812.
PROO88990.
SOURCESearch...

Entry information

Entry nameACTN3_MOUSE
AccessionPrimary (citable) accession number: O88990
Secondary accession number(s): Q14DS8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot