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Protein

Malate dehydrogenase, cytoplasmic

Gene

Mdh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei105NADBy similarity1
Binding sitei112NADBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei162SubstrateBy similarity1
Active sitei187Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADBy similarity7
Nucleotide bindingi129 – 131NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: RGD
  • malate dehydrogenase activity Source: RGD
  • NAD binding Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • malate metabolic process Source: RGD
  • mitophagy in response to mitochondrial depolarization Source: Ensembl
  • NADH metabolic process Source: RGD
  • NAD metabolic process Source: RGD
  • oxaloacetate metabolic process Source: RGD
  • positive regulation of defense response to virus by host Source: Ensembl
  • tricarboxylic acid cycle Source: GO_Central
  • xenophagy Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-RNO-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:Mdh1
Synonyms:Mdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3072. Mdh1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002267372 – 334Malate dehydrogenase, cytoplasmicAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei110N6-succinyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei121N6-acetyllysineBy similarity1
Modified residuei214N6-succinyllysineBy similarity1
Modified residuei217PhosphoserineCombined sources1
Modified residuei230Omega-N-methylarginineBy similarity1
Modified residuei241PhosphoserineCombined sources1
Modified residuei298N6-acetyllysine; alternateBy similarity1
Modified residuei298N6-succinyllysine; alternateBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei318N6-succinyllysineBy similarity1
Modified residuei332PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.By similarity
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88989.
PRIDEiO88989.

2D gel databases

World-2DPAGE0004:O88989.

PTM databases

iPTMnetiO88989.
PhosphoSitePlusiO88989.
SwissPalmiO88989.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008103.
GenevisibleiO88989. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi246700. 3 interactors.
IntActiO88989. 1 interactor.
MINTiMINT-4580226.
STRINGi10116.ENSRNOP00000011429.

Structurei

3D structure databases

ProteinModelPortaliO88989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiO88989.
KOiK00025.
OMAiQYPDAGQ.
OrthoDBiEOG091G0BLA.
PhylomeDBiO88989.
TreeFamiTF105826.

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGAALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA
Length:334
Mass (Da):36,483
Last modified:January 23, 2007 - v3
Checksum:i8F6778722A607B3C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251S → A in AAC26799 (Ref. 4) Curated1
Sequence conflicti276N → D in AAH59124 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093773 mRNA. Translation: AAC64180.1.
BC059124 mRNA. Translation: AAH59124.1.
AF075574 mRNA. Translation: AAC26799.1.
RefSeqiNP_150238.1. NM_033235.2.
UniGeneiRn.13492.

Genome annotation databases

EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103.
GeneIDi24551.
KEGGirno:24551.
UCSCiRGD:3072. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093773 mRNA. Translation: AAC64180.1.
BC059124 mRNA. Translation: AAH59124.1.
AF075574 mRNA. Translation: AAC26799.1.
RefSeqiNP_150238.1. NM_033235.2.
UniGeneiRn.13492.

3D structure databases

ProteinModelPortaliO88989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246700. 3 interactors.
IntActiO88989. 1 interactor.
MINTiMINT-4580226.
STRINGi10116.ENSRNOP00000011429.

PTM databases

iPTMnetiO88989.
PhosphoSitePlusiO88989.
SwissPalmiO88989.

2D gel databases

World-2DPAGE0004:O88989.

Proteomic databases

PaxDbiO88989.
PRIDEiO88989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103.
GeneIDi24551.
KEGGirno:24551.
UCSCiRGD:3072. rat.

Organism-specific databases

CTDi4190.
RGDi3072. Mdh1.

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiO88989.
KOiK00025.
OMAiQYPDAGQ.
OrthoDBiEOG091G0BLA.
PhylomeDBiO88989.
TreeFamiTF105826.

Enzyme and pathway databases

ReactomeiR-RNO-70263. Gluconeogenesis.

Miscellaneous databases

PROiO88989.

Gene expression databases

BgeeiENSRNOG00000008103.
GenevisibleiO88989. RN.

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHC_RAT
AccessioniPrimary (citable) accession number: O88989
Secondary accession number(s): O88585, Q6PCV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.