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O88989

- MDHC_RAT

UniProt

O88989 - MDHC_RAT

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Protein

Malate dehydrogenase, cytoplasmic

Gene
Mdh1, Mdh
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921Substrate By similarity
Binding sitei98 – 981Substrate By similarity
Binding sitei105 – 1051NAD By similarity
Binding sitei112 – 1121NAD By similarity
Binding sitei131 – 1311Substrate By similarity
Binding sitei162 – 1621Substrate By similarity
Active sitei187 – 1871Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NAD By similarity
Nucleotide bindingi129 – 1313NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: RGD
  2. malate dehydrogenase activity Source: RGD
  3. NAD binding Source: RGD

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: RGD
  3. NADH metabolic process Source: RGD
  4. NAD metabolic process Source: RGD
  5. oxaloacetate metabolic process Source: RGD
  6. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_217998. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:Mdh1
Synonyms:Mdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi3072. Mdh1.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 334333Malate dehydrogenase, cytoplasmicUniRule annotationPRO_0000226737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei110 – 1101N6-succinyllysine By similarity
Modified residuei118 – 1181N6-acetyllysine By similarity
Modified residuei121 – 1211N6-acetyllysine By similarity
Modified residuei214 – 2141N6-succinyllysine By similarity
Modified residuei298 – 2981N6-acetyllysine; alternate By similarity
Modified residuei298 – 2981N6-succinyllysine; alternate By similarity
Modified residuei318 – 3181N6-succinyllysine By similarity
Modified residuei333 – 3331Phosphoserine By similarity

Post-translational modificationi

ISGylated By similarity.UniRule annotation
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88989.
PRIDEiO88989.

2D gel databases

World-2DPAGE0004:O88989.

PTM databases

PhosphoSiteiO88989.

Expressioni

Gene expression databases

GenevestigatoriO88989.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi246700. 4 interactions.
IntActiO88989. 1 interaction.
MINTiMINT-4580226.
STRINGi10116.ENSRNOP00000011429.

Structurei

3D structure databases

ProteinModelPortaliO88989.
SMRiO88989. Positions 2-334.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiO88989.
KOiK00025.
OMAiNCLIASK.
OrthoDBiEOG78H3TM.
PhylomeDBiO88989.
TreeFamiTF105826.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88989-1 [UniParc]FASTAAdd to Basket

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MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL    50
DGVLMELQDC ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD 100
LLKANVKIFK SQGAALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE 150
NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV 200
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI 250
SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 300
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA 334
Length:334
Mass (Da):36,483
Last modified:January 23, 2007 - v3
Checksum:i8F6778722A607B3C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti251 – 2511S → A in AAC26799. 1 Publication
Sequence conflicti276 – 2761N → D in AAH59124. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093773 mRNA. Translation: AAC64180.1.
BC059124 mRNA. Translation: AAH59124.1.
AF075574 mRNA. Translation: AAC26799.1.
RefSeqiNP_150238.1. NM_033235.1.
UniGeneiRn.13492.

Genome annotation databases

EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103.
GeneIDi24551.
KEGGirno:24551.
UCSCiRGD:3072. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093773 mRNA. Translation: AAC64180.1 .
BC059124 mRNA. Translation: AAH59124.1 .
AF075574 mRNA. Translation: AAC26799.1 .
RefSeqi NP_150238.1. NM_033235.1.
UniGenei Rn.13492.

3D structure databases

ProteinModelPortali O88989.
SMRi O88989. Positions 2-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246700. 4 interactions.
IntActi O88989. 1 interaction.
MINTi MINT-4580226.
STRINGi 10116.ENSRNOP00000011429.

PTM databases

PhosphoSitei O88989.

2D gel databases

World-2DPAGE 0004:O88989.

Proteomic databases

PaxDbi O88989.
PRIDEi O88989.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000011429 ; ENSRNOP00000011429 ; ENSRNOG00000008103 .
GeneIDi 24551.
KEGGi rno:24551.
UCSCi RGD:3072. rat.

Organism-specific databases

CTDi 4190.
RGDi 3072. Mdh1.

Phylogenomic databases

eggNOGi COG0039.
GeneTreei ENSGT00530000063410.
HOGENOMi HOG000220953.
HOVERGENi HBG006340.
InParanoidi O88989.
KOi K00025.
OMAi NCLIASK.
OrthoDBi EOG78H3TM.
PhylomeDBi O88989.
TreeFami TF105826.

Enzyme and pathway databases

Reactomei REACT_217998. Gluconeogenesis.

Miscellaneous databases

NextBioi 603654.

Gene expression databases

Genevestigatori O88989.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of rat cytosolic malate dehydrogenase."
    Achenbach P., Steinbrenner H., Reindl G., Seissler J.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. Lubec G., Afjehi-Sadat L., Diao W., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 7-32; 80-92; 126-157; 171-199; 206-230; 239-255; 299-310 AND 319-334, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  4. "Partial sequence of rat malate dehydrogenase (MDH) mRNA."
    Earley S.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 251-284.

Entry informationi

Entry nameiMDHC_RAT
AccessioniPrimary (citable) accession number: O88989
Secondary accession number(s): O88585, Q6PCV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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