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O88989

- MDHC_RAT

UniProt

O88989 - MDHC_RAT

Protein

Malate dehydrogenase, cytoplasmic

Gene

Mdh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei98 – 981SubstrateBy similarity
    Binding sitei105 – 1051NADBy similarity
    Binding sitei112 – 1121NADBy similarity
    Binding sitei131 – 1311SubstrateBy similarity
    Binding sitei162 – 1621SubstrateBy similarity
    Active sitei187 – 1871Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 177NADBy similarity
    Nucleotide bindingi129 – 1313NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: RGD
    2. malate dehydrogenase activity Source: RGD
    3. NAD binding Source: RGD

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: RGD
    3. NADH metabolic process Source: RGD
    4. NAD metabolic process Source: RGD
    5. oxaloacetate metabolic process Source: RGD
    6. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_217998. Gluconeogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
    Alternative name(s):
    Cytosolic malate dehydrogenase
    Gene namesi
    Name:Mdh1
    Synonyms:Mdh
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi3072. Mdh1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 334333Malate dehydrogenase, cytoplasmicPRO_0000226737Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei110 – 1101N6-succinyllysineBy similarity
    Modified residuei118 – 1181N6-acetyllysineBy similarity
    Modified residuei121 – 1211N6-acetyllysineBy similarity
    Modified residuei214 – 2141N6-succinyllysineBy similarity
    Modified residuei298 – 2981N6-acetyllysine; alternateBy similarity
    Modified residuei298 – 2981N6-succinyllysine; alternateBy similarity
    Modified residuei318 – 3181N6-succinyllysineBy similarity
    Modified residuei333 – 3331PhosphoserineBy similarity

    Post-translational modificationi

    ISGylated.By similarity
    Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiO88989.
    PRIDEiO88989.

    2D gel databases

    World-2DPAGE0004:O88989.

    PTM databases

    PhosphoSiteiO88989.

    Expressioni

    Gene expression databases

    GenevestigatoriO88989.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi246700. 4 interactions.
    IntActiO88989. 1 interaction.
    MINTiMINT-4580226.
    STRINGi10116.ENSRNOP00000011429.

    Structurei

    3D structure databases

    ProteinModelPortaliO88989.
    SMRiO88989. Positions 2-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    GeneTreeiENSGT00530000063410.
    HOGENOMiHOG000220953.
    HOVERGENiHBG006340.
    InParanoidiO88989.
    KOiK00025.
    OMAiNCLIASK.
    OrthoDBiEOG78H3TM.
    PhylomeDBiO88989.
    TreeFamiTF105826.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88989-1 [UniParc]FASTAAdd to Basket

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    MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL    50
    DGVLMELQDC ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD 100
    LLKANVKIFK SQGAALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE 150
    NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV 200
    KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI 250
    SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 300
    EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA 334
    Length:334
    Mass (Da):36,483
    Last modified:January 23, 2007 - v3
    Checksum:i8F6778722A607B3C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti251 – 2511S → A in AAC26799. 1 PublicationCurated
    Sequence conflicti276 – 2761N → D in AAH59124. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093773 mRNA. Translation: AAC64180.1.
    BC059124 mRNA. Translation: AAH59124.1.
    AF075574 mRNA. Translation: AAC26799.1.
    RefSeqiNP_150238.1. NM_033235.1.
    UniGeneiRn.13492.

    Genome annotation databases

    EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103.
    GeneIDi24551.
    KEGGirno:24551.
    UCSCiRGD:3072. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093773 mRNA. Translation: AAC64180.1 .
    BC059124 mRNA. Translation: AAH59124.1 .
    AF075574 mRNA. Translation: AAC26799.1 .
    RefSeqi NP_150238.1. NM_033235.1.
    UniGenei Rn.13492.

    3D structure databases

    ProteinModelPortali O88989.
    SMRi O88989. Positions 2-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246700. 4 interactions.
    IntActi O88989. 1 interaction.
    MINTi MINT-4580226.
    STRINGi 10116.ENSRNOP00000011429.

    PTM databases

    PhosphoSitei O88989.

    2D gel databases

    World-2DPAGE 0004:O88989.

    Proteomic databases

    PaxDbi O88989.
    PRIDEi O88989.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000011429 ; ENSRNOP00000011429 ; ENSRNOG00000008103 .
    GeneIDi 24551.
    KEGGi rno:24551.
    UCSCi RGD:3072. rat.

    Organism-specific databases

    CTDi 4190.
    RGDi 3072. Mdh1.

    Phylogenomic databases

    eggNOGi COG0039.
    GeneTreei ENSGT00530000063410.
    HOGENOMi HOG000220953.
    HOVERGENi HBG006340.
    InParanoidi O88989.
    KOi K00025.
    OMAi NCLIASK.
    OrthoDBi EOG78H3TM.
    PhylomeDBi O88989.
    TreeFami TF105826.

    Enzyme and pathway databases

    Reactomei REACT_217998. Gluconeogenesis.

    Miscellaneous databases

    NextBioi 603654.

    Gene expression databases

    Genevestigatori O88989.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of rat cytosolic malate dehydrogenase."
      Achenbach P., Steinbrenner H., Reindl G., Seissler J.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. Lubec G., Afjehi-Sadat L., Diao W., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 7-32; 80-92; 126-157; 171-199; 206-230; 239-255; 299-310 AND 319-334, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    4. "Partial sequence of rat malate dehydrogenase (MDH) mRNA."
      Earley S.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 251-284.

    Entry informationi

    Entry nameiMDHC_RAT
    AccessioniPrimary (citable) accession number: O88989
    Secondary accession number(s): O88585, Q6PCV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3