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O88989 (MDHC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, cytoplasmic

EC=1.1.1.37
Alternative name(s):
Cytosolic malate dehydrogenase
Gene names
Name:Mdh1
Synonyms:Mdh
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01517.

Post-translational modification

ISGylated By similarity. HAMAP-Rule MF_01517

Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation By similarity. HAMAP-Rule MF_01517

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 334333Malate dehydrogenase, cytoplasmic HAMAP-Rule MF_01517
PRO_0000226737

Regions

Nucleotide binding11 – 177NAD By similarity
Nucleotide binding129 – 1313NAD By similarity

Sites

Active site1871Proton acceptor By similarity
Binding site921Substrate By similarity
Binding site981Substrate By similarity
Binding site1051NAD By similarity
Binding site1121NAD By similarity
Binding site1311Substrate By similarity
Binding site1621Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1101N6-succinyllysine By similarity
Modified residue1181N6-acetyllysine By similarity
Modified residue1211N6-acetyllysine By similarity
Modified residue2141N6-succinyllysine By similarity
Modified residue2981N6-acetyllysine; alternate By similarity
Modified residue2981N6-succinyllysine; alternate By similarity
Modified residue3181N6-succinyllysine By similarity
Modified residue3331Phosphoserine By similarity

Experimental info

Sequence conflict2511S → A in AAC26799. Ref.4
Sequence conflict2761N → D in AAH59124. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O88989 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8F6778722A607B3C

FASTA33436,483
        10         20         30         40         50         60 
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC 

        70         80         90        100        110        120 
ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD LLKANVKIFK SQGAALEKYA 

       130        140        150        160        170        180 
KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTADDVKN 

       190        200        210        220        230        240 
VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL 

       250        260        270        280        290        300 
SSAMSAAKAI SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 

       310        320        330 
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of rat cytosolic malate dehydrogenase."
Achenbach P., Steinbrenner H., Reindl G., Seissler J.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
[3]Lubec G., Afjehi-Sadat L., Diao W., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 7-32; 80-92; 126-157; 171-199; 206-230; 239-255; 299-310 AND 319-334, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain, Hippocampus and Spinal cord.
[4]"Partial sequence of rat malate dehydrogenase (MDH) mRNA."
Earley S.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 251-284.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093773 mRNA. Translation: AAC64180.1.
BC059124 mRNA. Translation: AAH59124.1.
AF075574 mRNA. Translation: AAC26799.1.
RefSeqNP_150238.1. NM_033235.1.
UniGeneRn.13492.

3D structure databases

ProteinModelPortalO88989.
SMRO88989. Positions 2-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246700. 4 interactions.
IntActO88989. 1 interaction.
MINTMINT-4580226.
STRING10116.ENSRNOP00000011429.

PTM databases

PhosphoSiteO88989.

2D gel databases

World-2DPAGE0004:O88989.

Proteomic databases

PaxDbO88989.
PRIDEO88989.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103.
GeneID24551.
KEGGrno:24551.
UCSCRGD:3072. rat.

Organism-specific databases

CTD4190.
RGD3072. Mdh1.

Phylogenomic databases

eggNOGCOG0039.
GeneTreeENSGT00530000063410.
HOGENOMHOG000220953.
HOVERGENHBG006340.
InParanoidO88989.
KOK00025.
OMANCLIASK.
OrthoDBEOG78H3TM.
PhylomeDBO88989.
TreeFamTF105826.

Gene expression databases

GenevestigatorO88989.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603654.

Entry information

Entry nameMDHC_RAT
AccessionPrimary (citable) accession number: O88989
Secondary accession number(s): O88585, Q6PCV2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families