Reviewed, UniProtKB/Swiss-Prot O88989 (MDHC_RAT)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
Alternative name(s):
    Cytosolic malate dehydrogenase

Gene names

Name:	Mdh1
Synonyms:	Mdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 334	333	Malate dehydrogenase, cytoplasmic		PRO_0000226737
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	210	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	251	1	S → A in AAC26799. Ref.4
	Sequence conflict	276	1	N → D in AAH59124. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O88989-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8F6778722A607B3C	FASTA	334	36,483
	10 20 30 40 50 60 MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC 70 80 90 100 110 120 ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD LLKANVKIFK SQGAALEKYA 130 140 150 160 170 180 KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTADDVKN 190 200 210 220 230 240 VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL 250 260 270 280 290 300 SSAMSAAKAI SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 310 320 330 EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of rat cytosolic malate dehydrogenase." Achenbach P., Steinbrenner H., Reindl G., Seissler J. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	Lubec G., Afjehi-Sadat L., Diao W., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 7-32; 80-92; 126-157; 171-199; 206-230; 239-255; 299-310 AND 319-334, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[4]	"Partial sequence of rat malate dehydrogenase (MDH) mRNA." Earley S. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 251-284.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF093773 mRNA. Translation: AAC64180.1. BC059124 mRNA. Translation: AAH59124.1. AF075574 mRNA. Translation: AAC26799.1.
RefSeq	NP_150238.1.
UniGene	Rn.13492
3D structure databases
HSSP	HSSP built from PDB template 4MDH based on UniProtKB P11708.
SMR	O88989. Positions 1-333, 2-334.
ModBase	Search...
PTM databases
PhosphoSite	O88989.
Genome annotation databases
Ensembl	ENSRNOG00000008103. Rattus norvegicus. [Contig view]
GeneID	24551.
KEGG	rno:24551.
NMPDR	fig|10116.3.peg.10844.
Organism-specific databases
RGD	3072. Mdh1.
Phylogenomic databases
HOVERGEN	O88989.
Gene expression databases
ArrayExpress	O88989.
GermOnline	ENSRNOG00000008103. Rattus norvegicus.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR011274. Malate_DHase_NAD-dep_euk. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603654.

Entry information

Entry name

MDHC_RAT

Accession

Primary (citable) accession number: O88989
Secondary accession number(s): O88585, Q6PCV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)