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Protein

A-kinase anchor protein 3

Gene

Akap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.By similarity

GO - Molecular functioni

  • protein kinase A binding Source: MGI

GO - Biological processi

  • protein localization Source: MGI
  • regulation of protein kinase A signaling Source: GO_Central
  • transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 3
Short name:
AKAP-3
Alternative name(s):
A-kinase anchor protein 110 kDa
Short name:
AKAP 110
Protein kinase A-anchoring protein 3
Short name:
PRKA3
Gene namesi
Name:Akap3
Synonyms:Akap110
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1341149. Akap3.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • motile cilium Source: MGI
  • nucleus Source: MGI
  • sperm fibrous sheath Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864A-kinase anchor protein 3PRO_0000064527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei206 – 2061PhosphoserineBy similarity
Modified residuei405 – 4051PhosphoserineBy similarity
Modified residuei406 – 4061PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88987.
PaxDbiO88987.
PeptideAtlasiO88987.
PRIDEiO88987.

PTM databases

PhosphoSiteiO88987.

Expressioni

Gene expression databases

BgeeiO88987.
CleanExiMM_AKAP3.
GenevisibleiO88987. MM.

Interactioni

Subunit structurei

Interacts with ROPN1 AND ROPN1L.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BrapQ99MP83EBI-9033539,EBI-10818333

GO - Molecular functioni

  • protein kinase A binding Source: MGI

Protein-protein interaction databases

BioGridi198050. 1 interaction.
IntActiO88987. 2 interactions.
STRINGi10090.ENSMUSP00000093091.

Structurei

3D structure databases

ProteinModelPortaliO88987.
SMRiO88987. Positions 779-864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 13814PKA-RII subunit binding domainAdd
BLAST

Domaini

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

eggNOGiENOG410IEV9. Eukaryota.
ENOG411183Z. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO88987.
KOiK16520.
OMAiQYHLAQG.
OrthoDBiEOG7327N3.
TreeFamiTF105403.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRVDWLQS QSGVCKVGVY SPGDNQHQDW KMDTSTDPVR VLSWLRKDLE
60 70 80 90 100
KSTAGFQDSR FKPGESSFVE EVAYPVDQRK GFCVDYYNTT NKGSPGRLHF
110 120 130 140 150
EMSHKENPSQ GLISHVGNGG SIDEVSFYAN RLTNLVIAMA RKEINEKIHG
160 170 180 190 200
AENKCVHQSL YMGDEPTPHK SLSTVASELV NETVTACSKN ISSDKAPGSG
210 220 230 240 250
DRASGSSQAP GLRYTSTLKI KESTKEGKCP DDKPGTKKSF FYKEVFESRN
260 270 280 290 300
AGDAKEGGRS LPGDQKLFRT SPDNRPDDFS NSISQGIMTY ANSVVSDMMV
310 320 330 340 350
SIMKTLKIQV KDTTIATILL KKVLMKHAKE VVSDLIDSFM KNLHGVTGSL
360 370 380 390 400
MTDTDFVSAV KRSFFSHGSQ KATDIMDAML GKLYNVMFAK KFPENIRRAR
410 420 430 440 450
DKSESYSLIS TKSRAGDPKL SNLNFAMKSE SKLKENLFST CKLEKEKTCA
460 470 480 490 500
ETLGEHIIKE GLHMWHKSQQ KSPGLERAAK LGNAPQEVSF ECPDPCEANP
510 520 530 540 550
PHQPQPPENF ANFMCDSDSW AKDLIVSALL LIQYHLAQGG KMDAQSFLEA
560 570 580 590 600
AASTNFPTNK PPPPSPVVQD ECKLKSPPHK ICDQEQTEKK DLMSVIFNFI
610 620 630 640 650
RNLLSETIFK SSRNCESNVH EQNTQEEEIH PCERPKTPCE RPITPPAPKF
660 670 680 690 700
CEDEEATGGA LSGLTKMVAN QLDNCMNGQM VEHLMDSVMK LCLIIAKSCD
710 720 730 740 750
SPLSELGEEK CGDASRPNSA FPDNLYECLP VKGTGTAEAL LQNAYLTIHN
760 770 780 790 800
ELRGLSGQPP EGCEIPKVIV SNHNLADTVQ NKQLQAVLQW VAASELNVPI
810 820 830 840 850
LYFAGDDEGI QEKLLQLSAT AVEKGRSVGE VLQSVLRYEK ERQLDEAVGN
860
VTRLQLLDWL MANL
Length:864
Mass (Da):95,556
Last modified:June 28, 2011 - v2
Checksum:i3E24E63327BC4E78
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2151T → M in AAC63369 (PubMed:10319321).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093406 mRNA. Translation: AAC63369.1.
CH466523 Genomic DNA. Translation: EDK99845.1.
BC100458 mRNA. Translation: AAI00459.1.
CCDSiCCDS20558.1.
RefSeqiNP_033780.2. NM_009650.2.
UniGeneiMm.87748.

Genome annotation databases

EnsembliENSMUST00000095440; ENSMUSP00000093091; ENSMUSG00000030344.
ENSMUST00000202574; ENSMUSP00000144405; ENSMUSG00000030344.
ENSMUST00000202878; ENSMUSP00000143794; ENSMUSG00000030344.
GeneIDi11642.
KEGGimmu:11642.
UCSCiuc009dvf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093406 mRNA. Translation: AAC63369.1.
CH466523 Genomic DNA. Translation: EDK99845.1.
BC100458 mRNA. Translation: AAI00459.1.
CCDSiCCDS20558.1.
RefSeqiNP_033780.2. NM_009650.2.
UniGeneiMm.87748.

3D structure databases

ProteinModelPortaliO88987.
SMRiO88987. Positions 779-864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198050. 1 interaction.
IntActiO88987. 2 interactions.
STRINGi10090.ENSMUSP00000093091.

PTM databases

PhosphoSiteiO88987.

Proteomic databases

MaxQBiO88987.
PaxDbiO88987.
PeptideAtlasiO88987.
PRIDEiO88987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095440; ENSMUSP00000093091; ENSMUSG00000030344.
ENSMUST00000202574; ENSMUSP00000144405; ENSMUSG00000030344.
ENSMUST00000202878; ENSMUSP00000143794; ENSMUSG00000030344.
GeneIDi11642.
KEGGimmu:11642.
UCSCiuc009dvf.2. mouse.

Organism-specific databases

CTDi10566.
MGIiMGI:1341149. Akap3.

Phylogenomic databases

eggNOGiENOG410IEV9. Eukaryota.
ENOG411183Z. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO88987.
KOiK16520.
OMAiQYHLAQG.
OrthoDBiEOG7327N3.
TreeFamiTF105403.

Miscellaneous databases

PROiO88987.
SOURCEiSearch...

Gene expression databases

BgeeiO88987.
CleanExiMM_AKAP3.
GenevisibleiO88987. MM.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein."
    Vijayaraghavan S., Liberty G.A., Mohan J., Winfrey V.P., Olson G.E., Carr D.W.
    Mol. Endocrinol. 13:705-717(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA."
    Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S.
    J. Biol. Chem. 276:17332-17338(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiAKAP3_MOUSE
AccessioniPrimary (citable) accession number: O88987
Secondary accession number(s): Q497M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 28, 2011
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.