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Protein

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

Gene

Gcat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate.

Cofactori

Pathwayi: L-threonine degradation via oxydo-reductase pathway

This protein is involved in step 2 of the subpathway that synthesizes glycine from L-threonine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-threonine 3-dehydrogenase, mitochondrial (Tdh)
  2. 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (Gcat)
This subpathway is part of the pathway L-threonine degradation via oxydo-reductase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561SubstrateBy similarity
Binding sitei203 – 2031Pyridoxal phosphateBy similarity
Binding sitei386 – 3861SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00046; UER00506.

Names & Taxonomyi

Protein namesi
Recommended name:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (EC:2.3.1.29)
Short name:
AKB ligase
Alternative name(s):
Aminoacetone synthase
Glycine acetyltransferase
Gene namesi
Name:Gcat
Synonyms:Kbl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1349389. Gcat.

Subcellular locationi

  • Mitochondrion By similarity
  • Nucleus By similarity

  • Note: Translocates to the nucleus upon cold and osmotic stress.By similarity

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818MitochondrionSequence analysisAdd
BLAST
Chaini19 – 4163982-amino-3-ketobutyrate coenzyme A ligase, mitochondrialPRO_0000001247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421N6-acetyllysine; alternateCombined sources
Modified residuei42 – 421N6-succinyllysine; alternateCombined sources
Modified residuei184 – 1841N6-acetyllysine; alternateCombined sources
Modified residuei184 – 1841N6-succinyllysine; alternateCombined sources
Modified residuei262 – 2621N6-(pyridoxal phosphate)lysineCurated
Modified residuei323 – 3231N6-succinyllysineCombined sources
Modified residuei365 – 3651N6-succinyllysineCombined sources
Modified residuei380 – 3801N6-acetyllysine; alternateCombined sources
Modified residuei380 – 3801N6-succinyllysine; alternateCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88986.
MaxQBiO88986.
PaxDbiO88986.
PeptideAtlasiO88986.
PRIDEiO88986.

2D gel databases

REPRODUCTION-2DPAGEO88986.
SWISS-2DPAGEO88986.

PTM databases

iPTMnetiO88986.
PhosphoSiteiO88986.
SwissPalmiO88986.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006378.
ExpressionAtlasiO88986. baseline and differential.
GenevisibleiO88986. MM.

Interactioni

Protein-protein interaction databases

MINTiMINT-1838793.
STRINGi10090.ENSMUSP00000006544.

Structurei

3D structure databases

ProteinModelPortaliO88986.
SMRiO88986. Positions 28-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1322Pyridoxal phosphate bindingBy similarity
Regioni259 – 2624Pyridoxal phosphate bindingBy similarity
Regioni292 – 2932Pyridoxal phosphate binding; shared with dimeric partnerBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1359. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221022.
HOVERGENiHBG105208.
InParanoidiO88986.
KOiK00639.
OrthoDBiEOG091G041D.
PhylomeDBiO88986.
TreeFamiTF105923.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00985. 2am3keto_CoA_ligase. 1 hit.
InterProiIPR011282. 2am3keto_CoA_ligase.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01822. 2am3keto_CoA. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWASFMWHGA LSPGRRAHSA LAQLRCILDS ELEGIRGAGT WKSERVITSR
60 70 80 90 100
QGPSIRVDGI SGGILNFCAN NYLGLSSHPA VIQAGLQTLE EFGAGLSSTR
110 120 130 140 150
FICGTQSIHK NLEAKIAHFH QREDAILYPS CFDANAGLFE ALLTPEDAVL
160 170 180 190 200
SDELNHASII DGIRLCKAHK YRYRHLDMAD LEAKLKEAQK HRLRLVATDG
210 220 230 240 250
AFSMDGDIAP LQDICRLAAQ YGALVFVDEC HATGFLGPTG RGTDELLGVM
260 270 280 290 300
DQVTIINSTL GKALGGASGG YTTGPEPLVS LLRQRSRPYL FSNSLPPAVV
310 320 330 340 350
GCASKALDLL MESNAIIQSM AAKTRRFRSK MEAAGFTVSG ADHPICPVML
360 370 380 390 400
GDARLSSQMA DDMLKKGIFV IGFSYPVVPK GKARIRVQIS AVHSEEDIDR
410
CVEAFVEVGR LHGALP
Length:416
Mass (Da):44,931
Last modified:January 1, 1999 - v2
Checksum:i01EA71B3846BEBBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093403 mRNA. Translation: AAC99774.1.
AK087433 mRNA. Translation: BAC39872.1.
BC024107 mRNA. Translation: AAH24107.1.
CCDSiCCDS27630.1.
RefSeqiNP_038875.3. NM_013847.4.
UniGeneiMm.237085.

Genome annotation databases

EnsembliENSMUST00000006544; ENSMUSP00000006544; ENSMUSG00000006378.
GeneIDi26912.
KEGGimmu:26912.
UCSCiuc007wse.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093403 mRNA. Translation: AAC99774.1.
AK087433 mRNA. Translation: BAC39872.1.
BC024107 mRNA. Translation: AAH24107.1.
CCDSiCCDS27630.1.
RefSeqiNP_038875.3. NM_013847.4.
UniGeneiMm.237085.

3D structure databases

ProteinModelPortaliO88986.
SMRiO88986. Positions 28-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1838793.
STRINGi10090.ENSMUSP00000006544.

PTM databases

iPTMnetiO88986.
PhosphoSiteiO88986.
SwissPalmiO88986.

2D gel databases

REPRODUCTION-2DPAGEO88986.
SWISS-2DPAGEO88986.

Proteomic databases

EPDiO88986.
MaxQBiO88986.
PaxDbiO88986.
PeptideAtlasiO88986.
PRIDEiO88986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006544; ENSMUSP00000006544; ENSMUSG00000006378.
GeneIDi26912.
KEGGimmu:26912.
UCSCiuc007wse.2. mouse.

Organism-specific databases

CTDi23464.
MGIiMGI:1349389. Gcat.

Phylogenomic databases

eggNOGiKOG1359. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221022.
HOVERGENiHBG105208.
InParanoidiO88986.
KOiK00639.
OrthoDBiEOG091G041D.
PhylomeDBiO88986.
TreeFamiTF105923.

Enzyme and pathway databases

UniPathwayiUPA00046; UER00506.

Miscellaneous databases

ChiTaRSiGcat. mouse.
PROiO88986.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006378.
ExpressionAtlasiO88986. baseline and differential.
GenevisibleiO88986. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00985. 2am3keto_CoA_ligase. 1 hit.
InterProiIPR011282. 2am3keto_CoA_ligase.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01822. 2am3keto_CoA. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKBL_MOUSE
AccessioniPrimary (citable) accession number: O88986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.