O88983 (STX8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-8

Alternative name(s):
Syntaxin-like protein 3I35

Gene names

Name:

Stx8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER By similarity.
Subunit structure	Forms a SNARE complex with STX7, VTI1B and VAMP8 which functions in the homotypic fusion of late endosomes. Part of the SNARE core complex containing STX7, VAMP8 and VTI1B. Interacts with VAMP8 By similarity. Interacts with HECTD3. Ref.7
Subcellular location	Membrane; Single-pass type IV membrane protein By similarity. Note: Preferentially associated with the early endosome. To lesser extends, also present in late endosome, the plasma membrane and coated pits By similarity.
Post-translational modification	Ubiquitinated by HECTD3. Ref.7
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin protein ligase binding Inferred from physical interaction Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Syntaxin-8		PRO_0000210218
Regions
	Topological domain	1 – 215	215	Cytoplasmic Potential
	Transmembrane	216 – 232	17	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	233 – 236	4	Vesicular Potential
	Domain	145 – 207	63	t-SNARE coiled-coil homology
	Coiled coil	42 – 65	24	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88983 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: EAE1D9D150C2EF2D	FASTA	236	26,925
	10 20 30 40 50 60 MAPDPWFSTY DSTCQIAQEI AEKIQERNQC ERRGEKTPKL TLTIRTLLKN LKVKIDLLKD 70 80 90 100 110 120 LLLRAVSTRQ ITQLEGDRRQ NLLDDLVTRE RLLLASFKNE GAEPDLIRSS LMSEEAKRGT 130 140 150 160 170 180 PNPWLCEEPE ETRGLGFDEI RQQQQKIIQE QDAGLDALSS IISRQKQMGQ EIGNELDEQN 190 200 210 220 230 EIIDDLANLV ENTDEKLRTE ARRVTLVDRK STSCGMIMVI LLLLVAIVVV AVWPTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Thoreau V., Bilan F., Kitzis A., Chomel J.-C. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Subramaniam V.N., Loh E., Hong W. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Mouse syntaxin8." Nakamura N., Wada Y., Futai M. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[5]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain and Testis.
[7]	"Interaction between syntaxin 8 and HECTd3, a HECT domain ligase." Zhang L., Kang L., Bond W., Zhang N. Cell. Mol. Neurobiol. 29:115-121(2009) [PubMed: 18821010] [Abstract] Cited for: INTERACTION WITH HECTD3, UBIQUITINATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093064 mRNA. Translation: AAC64149.1. AF036716 mRNA. Translation: AAC95286.1. AB040054 mRNA. Translation: BAB20500.1. AK003297 mRNA. Translation: BAB22698.1. AL669842, AL663076 Genomic DNA. Translation: CAI24774.1. BC048167 mRNA. Translation: AAH48167.1. BC048479 mRNA. Translation: AAH48479.1.
IPI	IPI00136653.
RefSeq	NP_061238.1. NM_018768.2.
UniGene	Mm.3973.
3D structure databases
ProteinModelPortal	O88983.
SMR	O88983. Positions 152-205.
ModBase	Search...
Protein-protein interaction databases
STRING	O88983.
PTM databases
PhosphoSite	O88983.
Proteomic databases
PRIDE	O88983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021285; ENSMUSP00000021285; ENSMUSG00000020903.
GeneID	55943.
KEGG	mmu:55943.
Organism-specific databases
CTD	9482.
MGI	MGI:1890156. Stx8.
Phylogenomic databases
eggNOG	roNOG06719.
HOGENOM	HBG716370.
HOVERGEN	HBG007572.
InParanoid	O88983.
OMA	QKIIQEQ.
OrthoDB	EOG402WT8.
PhylomeDB	O88983.
Gene expression databases
ArrayExpress	O88983.
Bgee	O88983.
CleanEx	MM_STX8.
Genevestigator	O88983.
GermOnline	ENSMUSG00000020903. Mus musculus.
Family and domain databases
InterPro	IPR015640. Syntax_8. IPR000727. T_SNARE_dom. [Graphical view]
KO	K08501.
PANTHER	PTHR12380:SF6. Syntax_8. 1 hit.
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00397. t_SNARE. 1 hit. [Graphical view]
PROSITE	PS00914. SYNTAXIN. False negative. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311626.
SOURCE	Search...

Entry information

Entry name

STX8_MOUSE

Accession

Primary (citable) accession number: O88983

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program