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O88962 (CP8B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

EC=1.14.13.95
Alternative name(s):
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
CYPVIIIB1
Cytochrome P450 8B1
Sterol 12-alpha-hydroxylase
Gene names
Name:Cyp8b1
Synonyms:Cyp12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

Catalytic activity

7-alpha-hydroxycholest-4-en-3-one + NADPH + O2 = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Tissue specificity

Expressed in liver.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 5004997-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
PRO_0000051914

Regions

Transmembrane4 – 2421Helical; Potential

Sites

Metal binding4391Iron (heme axial ligand) By similarity

Natural variations

Natural variant2421E → K in strain: 129/SvJ. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O88962 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: D6902755B4AD141A

FASTA50057,706
        10         20         30         40         50         60 
MTLWCTVLGA LLTVVGCLCL SLLLRHRRPW EPPLDKGFVP WLGHSMAFRK NMFEFLKGMR 

        70         80         90        100        110        120 
AKHGDVFTVQ LGGQYFTFVM DPLSFGPIIK NTEKALDFQS YAKELVLKVF GYQSVDGDHR 

       130        140        150        160        170        180 
MIHLASTKHL MGQGLEELNQ AMLDSLSLVM LGPKGSSLGA SSWCEDGLFH FCYRILFKAG 

       190        200        210        220        230        240 
FLSLFGYTKD KQQDLDEADE LFRKFRRFDF LFPRFVYSLL GPREWVEVSQ LQRLFHQRLS 

       250        260        270        280        290        300 
VEQNLEKDGI SCWLGYMLQF LREQGIASSM QDKFNFMMLW ASQGNTGPTC FWVLLFLLKH 

       310        320        330        340        350        360 
QDAMKAVREE ATRVMGKARL EAKKSFTFTP SALKHTPVLD SVMEESLRLC ATPTLLRVVQ 

       370        380        390        400        410        420 
EDYVLKMASG QEYQIRRGDK VALFPYLSVH MDPDIHPEPT AFKYDRFLNP DGTRKVDFYK 

       430        440        450        460        470        480 
SGKKIHHYSM PWGSGVSKCP GRFFALSEMK TFVLLMIMYF DFKLVDPDIP VPPIDPRRWG 

       490        500 
FGTSQPSHEV RFLYRLKPVQ 

« Hide

References

« Hide 'large scale' references
[1]"Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns."
Gaafvels M., Olin M., Chowdhary B.P., Raudsepp T., Andersson U., Persson B., Jansson M., Bjoerkhem I., Eggertsen G.
Genomics 56:184-196(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT LYS-242.
Strain: 129/SvJ and BALB/c.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF090317 mRNA. Translation: AAC63036.1.
AF090319 Genomic DNA. Translation: AAD19876.1.
BC010973 mRNA. Translation: AAH10973.1.
BC010974 mRNA. Translation: AAH10974.1.
BC049969 mRNA. Translation: AAH49969.1.
CCDSCCDS23641.1.
RefSeqNP_034142.3. NM_010012.3.
UniGeneMm.20889.

3D structure databases

ProteinModelPortalO88962.
SMRO88962. Positions 25-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1865660.
STRING10090.ENSMUSP00000052989.

PTM databases

PhosphoSiteO88962.

Proteomic databases

PaxDbO88962.
PRIDEO88962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062474; ENSMUSP00000052989; ENSMUSG00000050445.
GeneID13124.
KEGGmmu:13124.
UCSCuc009sef.2. mouse.

Organism-specific databases

CTD1582.
MGIMGI:1338044. Cyp8b1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00550000074551.
HOGENOMHOG000231026.
HOVERGENHBG051100.
InParanoidO88962.
KOK07431.
OrthoDBEOG7J9VP6.
PhylomeDBO88962.
TreeFamTF105090.

Gene expression databases

BgeeO88962.
GenevestigatorO88962.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PIRSFPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSPR00465. EP450IV.
SUPFAMSSF48264. SSF48264. 1 hit.
ProtoNetSearch...

Other

NextBio283170.
PROO88962.
SOURCESearch...

Entry information

Entry nameCP8B1_MOUSE
AccessionPrimary (citable) accession number: O88962
Secondary accession number(s): Q9R217
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot