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O88962

- CP8B1_MOUSE

UniProt

O88962 - CP8B1_MOUSE

Protein

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Gene

Cyp8b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

    Catalytic activityi

    7-alpha-hydroxycholest-4-en-3-one + NADPH + O2 = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity Source: UniProtKB-EC
    2. heme binding Source: RefGenome
    3. iron ion binding Source: InterPro
    4. sterol 12-alpha-hydroxylase activity Source: RefGenome

    GO - Biological processi

    1. lipid metabolic process Source: RefGenome

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    REACT_227297. Sterols are 12-hydroxylated by CYP8B1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC:1.14.13.95)
    Alternative name(s):
    7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
    CYPVIIIB1
    Cytochrome P450 8B1
    Sterol 12-alpha-hydroxylase
    Gene namesi
    Name:Cyp8b1
    Synonyms:Cyp12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1338044. Cyp8b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: RefGenome
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 5004997-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylasePRO_0000051914Add
    BLAST

    Proteomic databases

    PaxDbiO88962.
    PRIDEiO88962.

    PTM databases

    PhosphoSiteiO88962.

    Expressioni

    Tissue specificityi

    Expressed in liver.

    Gene expression databases

    BgeeiO88962.
    GenevestigatoriO88962.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1865660.
    STRINGi10090.ENSMUSP00000052989.

    Structurei

    3D structure databases

    ProteinModelPortaliO88962.
    SMRiO88962. Positions 25-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00550000074551.
    HOGENOMiHOG000231026.
    HOVERGENiHBG051100.
    InParanoidiO88962.
    KOiK07431.
    OrthoDBiEOG7J9VP6.
    PhylomeDBiO88962.
    TreeFamiTF105090.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSiPR00465. EP450IV.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88962-1 [UniParc]FASTAAdd to Basket

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    MTLWCTVLGA LLTVVGCLCL SLLLRHRRPW EPPLDKGFVP WLGHSMAFRK    50
    NMFEFLKGMR AKHGDVFTVQ LGGQYFTFVM DPLSFGPIIK NTEKALDFQS 100
    YAKELVLKVF GYQSVDGDHR MIHLASTKHL MGQGLEELNQ AMLDSLSLVM 150
    LGPKGSSLGA SSWCEDGLFH FCYRILFKAG FLSLFGYTKD KQQDLDEADE 200
    LFRKFRRFDF LFPRFVYSLL GPREWVEVSQ LQRLFHQRLS VEQNLEKDGI 250
    SCWLGYMLQF LREQGIASSM QDKFNFMMLW ASQGNTGPTC FWVLLFLLKH 300
    QDAMKAVREE ATRVMGKARL EAKKSFTFTP SALKHTPVLD SVMEESLRLC 350
    ATPTLLRVVQ EDYVLKMASG QEYQIRRGDK VALFPYLSVH MDPDIHPEPT 400
    AFKYDRFLNP DGTRKVDFYK SGKKIHHYSM PWGSGVSKCP GRFFALSEMK 450
    TFVLLMIMYF DFKLVDPDIP VPPIDPRRWG FGTSQPSHEV RFLYRLKPVQ 500
    Length:500
    Mass (Da):57,706
    Last modified:November 1, 1998 - v1
    Checksum:iD6902755B4AD141A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti242 – 2421E → K in strain: 129/SvJ. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090317 mRNA. Translation: AAC63036.1.
    AF090319 Genomic DNA. Translation: AAD19876.1.
    BC010973 mRNA. Translation: AAH10973.1.
    BC010974 mRNA. Translation: AAH10974.1.
    BC049969 mRNA. Translation: AAH49969.1.
    CCDSiCCDS23641.1.
    RefSeqiNP_034142.3. NM_010012.3.
    UniGeneiMm.20889.

    Genome annotation databases

    EnsembliENSMUST00000062474; ENSMUSP00000052989; ENSMUSG00000050445.
    GeneIDi13124.
    KEGGimmu:13124.
    UCSCiuc009sef.2. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090317 mRNA. Translation: AAC63036.1 .
    AF090319 Genomic DNA. Translation: AAD19876.1 .
    BC010973 mRNA. Translation: AAH10973.1 .
    BC010974 mRNA. Translation: AAH10974.1 .
    BC049969 mRNA. Translation: AAH49969.1 .
    CCDSi CCDS23641.1.
    RefSeqi NP_034142.3. NM_010012.3.
    UniGenei Mm.20889.

    3D structure databases

    ProteinModelPortali O88962.
    SMRi O88962. Positions 25-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1865660.
    STRINGi 10090.ENSMUSP00000052989.

    PTM databases

    PhosphoSitei O88962.

    Proteomic databases

    PaxDbi O88962.
    PRIDEi O88962.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000062474 ; ENSMUSP00000052989 ; ENSMUSG00000050445 .
    GeneIDi 13124.
    KEGGi mmu:13124.
    UCSCi uc009sef.2. mouse.

    Organism-specific databases

    CTDi 1582.
    MGIi MGI:1338044. Cyp8b1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00550000074551.
    HOGENOMi HOG000231026.
    HOVERGENi HBG051100.
    InParanoidi O88962.
    KOi K07431.
    OrthoDBi EOG7J9VP6.
    PhylomeDBi O88962.
    TreeFami TF105090.

    Enzyme and pathway databases

    Reactomei REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    REACT_227297. Sterols are 12-hydroxylated by CYP8B1.

    Miscellaneous databases

    NextBioi 283170.
    PROi O88962.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88962.
    Genevestigatori O88962.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSi PR00465. EP450IV.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns."
      Gaafvels M., Olin M., Chowdhary B.P., Raudsepp T., Andersson U., Persson B., Jansson M., Bjoerkhem I., Eggertsen G.
      Genomics 56:184-196(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT LYS-242.
      Strain: 129/SvJ and BALB/c.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP8B1_MOUSE
    AccessioniPrimary (citable) accession number: O88962
    Secondary accession number(s): Q9R217
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3