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Protein

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Gene

Cyp8b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

Catalytic activityi

7-alpha-hydroxycholest-4-en-3-one + NADPH + O2 = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_283453. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_292237. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_347652. Sterols are 12-hydroxylated by CYP8B1.

Names & Taxonomyi

Protein namesi
Recommended name:
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC:1.14.13.95)
Alternative name(s):
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
CYPVIIIB1
Cytochrome P450 8B1
Sterol 12-alpha-hydroxylase
Gene namesi
Name:Cyp8b1
Synonyms:Cyp12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1338044. Cyp8b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 5004997-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylasePRO_0000051914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88962.
PRIDEiO88962.

PTM databases

PhosphoSiteiO88962.

Expressioni

Tissue specificityi

Expressed in liver.

Gene expression databases

BgeeiO88962.
GenevestigatoriO88962.

Interactioni

Protein-protein interaction databases

MINTiMINT-1865660.
STRINGi10090.ENSMUSP00000052989.

Structurei

3D structure databases

ProteinModelPortaliO88962.
SMRiO88962. Positions 25-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO88962.
KOiK07431.
OrthoDBiEOG7J9VP6.
PhylomeDBiO88962.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR030686. Cytochrome_CYP8B1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500627. Cytochrome_CYP8B1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLWCTVLGA LLTVVGCLCL SLLLRHRRPW EPPLDKGFVP WLGHSMAFRK
60 70 80 90 100
NMFEFLKGMR AKHGDVFTVQ LGGQYFTFVM DPLSFGPIIK NTEKALDFQS
110 120 130 140 150
YAKELVLKVF GYQSVDGDHR MIHLASTKHL MGQGLEELNQ AMLDSLSLVM
160 170 180 190 200
LGPKGSSLGA SSWCEDGLFH FCYRILFKAG FLSLFGYTKD KQQDLDEADE
210 220 230 240 250
LFRKFRRFDF LFPRFVYSLL GPREWVEVSQ LQRLFHQRLS VEQNLEKDGI
260 270 280 290 300
SCWLGYMLQF LREQGIASSM QDKFNFMMLW ASQGNTGPTC FWVLLFLLKH
310 320 330 340 350
QDAMKAVREE ATRVMGKARL EAKKSFTFTP SALKHTPVLD SVMEESLRLC
360 370 380 390 400
ATPTLLRVVQ EDYVLKMASG QEYQIRRGDK VALFPYLSVH MDPDIHPEPT
410 420 430 440 450
AFKYDRFLNP DGTRKVDFYK SGKKIHHYSM PWGSGVSKCP GRFFALSEMK
460 470 480 490 500
TFVLLMIMYF DFKLVDPDIP VPPIDPRRWG FGTSQPSHEV RFLYRLKPVQ
Length:500
Mass (Da):57,706
Last modified:November 1, 1998 - v1
Checksum:iD6902755B4AD141A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti242 – 2421E → K in strain: 129/SvJ. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090317 mRNA. Translation: AAC63036.1.
AF090319 Genomic DNA. Translation: AAD19876.1.
BC010973 mRNA. Translation: AAH10973.1.
BC010974 mRNA. Translation: AAH10974.1.
BC049969 mRNA. Translation: AAH49969.1.
CCDSiCCDS23641.1.
RefSeqiNP_034142.3. NM_010012.3.
UniGeneiMm.20889.

Genome annotation databases

GeneIDi13124.
KEGGimmu:13124.
UCSCiuc009sef.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090317 mRNA. Translation: AAC63036.1.
AF090319 Genomic DNA. Translation: AAD19876.1.
BC010973 mRNA. Translation: AAH10973.1.
BC010974 mRNA. Translation: AAH10974.1.
BC049969 mRNA. Translation: AAH49969.1.
CCDSiCCDS23641.1.
RefSeqiNP_034142.3. NM_010012.3.
UniGeneiMm.20889.

3D structure databases

ProteinModelPortaliO88962.
SMRiO88962. Positions 25-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1865660.
STRINGi10090.ENSMUSP00000052989.

PTM databases

PhosphoSiteiO88962.

Proteomic databases

PaxDbiO88962.
PRIDEiO88962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13124.
KEGGimmu:13124.
UCSCiuc009sef.2. mouse.

Organism-specific databases

CTDi1582.
MGIiMGI:1338044. Cyp8b1.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO88962.
KOiK07431.
OrthoDBiEOG7J9VP6.
PhylomeDBiO88962.
TreeFamiTF105090.

Enzyme and pathway databases

ReactomeiREACT_283453. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_292237. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_347652. Sterols are 12-hydroxylated by CYP8B1.

Miscellaneous databases

NextBioi283170.
PROiO88962.
SOURCEiSearch...

Gene expression databases

BgeeiO88962.
GenevestigatoriO88962.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR030686. Cytochrome_CYP8B1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500627. Cytochrome_CYP8B1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns."
    Gaafvels M., Olin M., Chowdhary B.P., Raudsepp T., Andersson U., Persson B., Jansson M., Bjoerkhem I., Eggertsen G.
    Genomics 56:184-196(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT LYS-242.
    Strain: 129/SvJ and BALB/c.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiCP8B1_MOUSE
AccessioniPrimary (citable) accession number: O88962
Secondary accession number(s): Q9R217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: May 27, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.