Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucosamine-6-phosphate isomerase 1

Gene

Gnpda1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity).By similarity

Catalytic activityi

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Proton acceptor; for enolization stepBy similarity
Active sitei141 – 1411For ring-opening stepBy similarity
Active sitei143 – 1431Proton acceptor; for ring-opening stepBy similarity
Active sitei148 – 1481For ring-opening stepBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-MMU-70326. Glucose metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine-6-phosphate isomerase 1 (EC:3.5.99.6)
Alternative name(s):
Glucosamine-6-phosphate deaminase 1
Short name:
GNPDA 1
Short name:
GlcN6P deaminase 1
Oscillin
Gene namesi
Name:Gnpda1
Synonyms:Gnpi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1347054. Gnpda1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Glucosamine-6-phosphate isomerase 1PRO_0000160124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei161 – 1611PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88958.
PaxDbiO88958.
PRIDEiO88958.

PTM databases

iPTMnetiO88958.
PhosphoSiteiO88958.

Expressioni

Tissue specificityi

Widely expressed. Detected in brain, liver, kidney, muscle, ovary, testis, spermatids and spermatozoa. In spermatids, located close to the developing acrosome vesicle. In spermatozoa, found close to the acrosomal region.1 Publication

Gene expression databases

CleanExiMM_GNPDA1.
ExpressionAtlasiO88958. baseline.
GenevisibleiO88958. MM.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiO88958. 1 interaction.
MINTiMINT-4121378.
STRINGi10090.ENSMUSP00000069081.

Structurei

3D structure databases

ProteinModelPortaliO88958.
SMRiO88958. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiO88958.
KOiK02564.
OMAiFFDNDMS.
OrthoDBiEOG7NW69T.
TreeFamiTF300841.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIILEHYS QASEWAAKYI RNRIIQFNPG PDKYFTLGLP TGSTPLGCYQ
60 70 80 90 100
KLIEYYKNGD LSFQYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH
110 120 130 140 150
PENTHILDGN AADLQAECDA FEEKIQAAGG IELFVGGIGP DGHIAFNEPG
160 170 180 190 200
SSLVSRTRVK TLAMDTILAN ARFFDGDLAK VPTMALTVGV GTVMDAKEVM
210 220 230 240 250
ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD EDATLELKVK
260 270 280
TVKYFKGLML VHNKLVDPLY SIKEKEIQKS QSAKKPYSD
Length:289
Mass (Da):32,549
Last modified:October 3, 2012 - v3
Checksum:i4778C0E0D74EBBB8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871S → F in AAC36739 (PubMed:10481053).Curated
Sequence conflicti91 – 911N → D in AAC36739 (PubMed:10481053).Curated
Sequence conflicti171 – 1711A → G in AAC36739 (PubMed:10481053).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088903 mRNA. Translation: AAC36739.1.
AF160355 mRNA. Translation: AAD42233.1.
AC134576 Genomic DNA. No translation available.
AC152450 Genomic DNA. No translation available.
CH466528 Genomic DNA. Translation: EDL10078.1.
BC014800 mRNA. Translation: AAH14800.1.
CCDSiCCDS29201.1.
RefSeqiNP_036067.2. NM_011937.2.
UniGeneiMm.22374.

Genome annotation databases

EnsembliENSMUST00000063814; ENSMUSP00000069081; ENSMUSG00000052102.
GeneIDi26384.
KEGGimmu:26384.
UCSCiuc008esi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088903 mRNA. Translation: AAC36739.1.
AF160355 mRNA. Translation: AAD42233.1.
AC134576 Genomic DNA. No translation available.
AC152450 Genomic DNA. No translation available.
CH466528 Genomic DNA. Translation: EDL10078.1.
BC014800 mRNA. Translation: AAH14800.1.
CCDSiCCDS29201.1.
RefSeqiNP_036067.2. NM_011937.2.
UniGeneiMm.22374.

3D structure databases

ProteinModelPortaliO88958.
SMRiO88958. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88958. 1 interaction.
MINTiMINT-4121378.
STRINGi10090.ENSMUSP00000069081.

PTM databases

iPTMnetiO88958.
PhosphoSiteiO88958.

Proteomic databases

EPDiO88958.
PaxDbiO88958.
PRIDEiO88958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063814; ENSMUSP00000069081; ENSMUSG00000052102.
GeneIDi26384.
KEGGimmu:26384.
UCSCiuc008esi.2. mouse.

Organism-specific databases

CTDi10007.
MGIiMGI:1347054. Gnpda1.

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiO88958.
KOiK02564.
OMAiFFDNDMS.
OrthoDBiEOG7NW69T.
TreeFamiTF300841.

Enzyme and pathway databases

ReactomeiR-MMU-70326. Glucose metabolism.

Miscellaneous databases

ChiTaRSiGnpda1. mouse.
PROiO88958.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GNPDA1.
ExpressionAtlasiO88958. baseline.
GenevisibleiO88958. MM.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of testicular mouse glucosamine 6-phosphate deaminase (GNPDA)."
    Montag M., van der Ven K., Doerbecker C., van der Ven H.
    FEBS Lett. 458:141-144(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Cloning, sequencing, and expression analysis of mouse glucosamine-6-phosphate deaminase (GNPDA/oscillin)."
    Amireault P., Dube F.
    Mol. Reprod. Dev. 56:424-435(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGNPI1_MOUSE
AccessioniPrimary (citable) accession number: O88958
Secondary accession number(s): Q91WJ4, Q9R188
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 3, 2012
Last modified: June 8, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.