Reviewed, UniProtKB/Swiss-Prot O88956 (LPP1_CAVPO)

Names and origin

Protein names

Recommended name:
    Lipid phosphate phosphohydrolase 1
    EC=3.1.3.4
Alternative name(s):
    Phosphatidic acid phosphatase 2a
    PAP2-alpha
      Short name=PAP-2a
      Short name=PAP2a
    Phosphatidate phosphohydrolase type 2a

Gene names

Name:	PPAP2A
Synonyms:	LPP1, PAP2A

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P) By similarity.
Catalytic activity	A 3-sn-phosphatidate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PA-phosphatase related phosphoesterase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidate phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O88956-1)

Also known as: PAP2a1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O88956-2)

Also known as: PAP2a2;

The sequence of this isoform differs from the canonical sequence as follows:
     21-70: GLPFAILTSR...GIMIPFSIVV → SMPMAVLNLG...IVGLGLPISS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Lipid phosphate phosphohydrolase 1		PRO_0000220904
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Potential
	Topological domain	28 – 53	26	Lumenal Potential
	Transmembrane	54 – 74	21	Potential
	Topological domain	75 – 94	20	Cytoplasmic Potential
	Transmembrane	95 – 115	21	Potential
	Topological domain	116 – 165	50	Lumenal Potential
	Transmembrane	166 – 186	21	Potential
	Topological domain	187 – 199	13	Cytoplasmic Potential
	Transmembrane	200 – 220	21	Potential
	Topological domain	221 – 229	9	Lumenal Potential
	Transmembrane	230 – 250	21	Potential
	Topological domain	251 – 285	35	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	21 – 70	50	GLPFA…FSIVV → SMPMAVLNLGQIYPFQRGFF CNDNSIQYPYHDSTVASTIL TIVGLGLPISS in isoform 2.		VSP_009650
Experimental info
	Sequence conflict	5	1	A → T in AAC63334. Ref.1
	Sequence conflict	218	1	I → V in AAC63334. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PAP2a1) [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E6F48E188DED6CF5	FASTA	285	32,133
	10 20 30 40 50 60 MFDKARLPYV ALDVLCVVLA GLPFAILTSR HTPFQRGIFC NDESIKYPYK EDTIPYALLG 70 80 90 100 110 120 GIMIPFSIVV MIIGETLSVY CNLLHSNSFI RNNYIATIYK SIGTFLFGAA ASQSLTDIAK 130 140 150 160 170 180 YSIGRLRPHF LSVCDPDWSK VNCSDGYIEY YVCRGNAEKV KEGRLSFYSG HSSFSMYCMV 190 200 210 220 230 240 FVALYLQARM KGDWARLLRP TLQFGLVAAS IYVGLSRISD YKHHWSDVLT GLIQGAIVAI 250 260 270 280 LVAVYVSDFF KARNSPFQER KEEDSHTTLH ETPTAGNHYR SNHQP
	Isoform 2 (PAP2a2). Checksum: 32A7CD8C19AE02FB	FASTA	286	32,081
	10 20 30 40 50 60 MFDKARLPYV ALDVLCVVLA SMPMAVLNLG QIYPFQRGFF CNDNSIQYPY HDSTVASTIL 70 80 90 100 110 120 TIVGLGLPIS SMIIGETLSV YCNLLHSNSF IRNNYIATIY KSIGTFLFGA AASQSLTDIA 130 140 150 160 170 180 KYSIGRLRPH FLSVCDPDWS KVNCSDGYIE YYVCRGNAEK VKEGRLSFYS GHSSFSMYCM 190 200 210 220 230 240 VFVALYLQAR MKGDWARLLR PTLQFGLVAA SIYVGLSRIS DYKHHWSDVL TGLIQGAIVA 250 260 270 280 ILVAVYVSDF FKARNSPFQE RKEEDSHTTL HETPTAGNHY RSNHQP

References

[1]	"Molecular cloning of magnesium-independent type 2 phosphatidic acid phosphatases from airway smooth muscle." Tate R.J., Tolan D., Pyne S. Cell. Signal. 11:515-522(1999) [PubMed: 10405762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Airway smooth muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF088283 mRNA. Translation: AAC63333.1. AF088284 mRNA. Translation: AAC63334.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOG00000009814. Cavia porcellus. [Contig view]
Phylogenomic databases
HOVERGEN	O88956.
Enzyme and pathway databases
BRENDA	3.1.3.4. 44.
Family and domain databases
InterPro	IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPP1_CAVPO

Accession

Primary (citable) accession number: O88956
Secondary accession number(s): O88957

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)