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Protein

Zinc finger and BTB domain-containing protein 7A

Gene

Zbtb7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri376 – 39823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri404 – 42623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri432 – 45423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri460 – 48425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • histone acetyltransferase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB

GO - Biological processi

  • cartilage development Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of osteoclast differentiation Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 7A
Alternative name(s):
Leukemia/lymphoma-related factor
POZ and Krueppel erythroid myeloid ontogenic factor
Short name:
POK erythroid myeloid ontogenic factor
Short name:
Pokemon
Gene namesi
Name:Zbtb7a
Synonyms:Lrf, Zbtb7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1335091. Zbtb7a.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death around 16.5 dpc because of severe anemia with a profound block in early B-cell development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Zinc finger and BTB domain-containing protein 7APRO_0000047716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei331 – 3311PhosphoserineCombined sources
Modified residuei335 – 3351PhosphoserineBy similarity
Cross-linki527 – 527Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei537 – 5371PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO88939.
MaxQBiO88939.
PaxDbiO88939.
PeptideAtlasiO88939.
PRIDEiO88939.

PTM databases

iPTMnetiO88939.
PhosphoSiteiO88939.

Expressioni

Tissue specificityi

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas.2 Publications

Developmental stagei

Expressed at E9.5-E10.0 day in limb buds, pharyngeal arches, tail bud, placenta and neural tube.1 Publication

Gene expression databases

BgeeiENSMUSG00000035011.
CleanExiMM_ZBTB7A.
ExpressionAtlasiO88939. baseline and differential.
GenevisibleiO88939. MM.

Interactioni

Subunit structurei

Interacts with BCL6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hdac1O091062EBI-595063,EBI-301912

GO - Molecular functioni

  • histone acetyltransferase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi201199. 8 interactions.
IntActiO88939. 8 interactions.
MINTiMINT-4140499.
STRINGi10090.ENSMUSP00000047333.

Structurei

3D structure databases

ProteinModelPortaliO88939.
SMRiO88939. Positions 9-162, 377-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 10168BTBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri376 – 39823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri404 – 42623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri432 – 45423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri460 – 48425C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129834.
HOGENOMiHOG000082521.
HOVERGENiHBG106747.
InParanoidiO88939.
KOiK10494.
OMAiQMMDSIE.
OrthoDBiEOG091G057O.
PhylomeDBiO88939.
TreeFamiTF331824.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88939-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS
60 70 80 90 100
VLAACSQYFK KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS
110 120 130 140 150
TANVGDILSA ARLLEIPAVS HVCADLLERQ ILAADDVGDA SQPDGAGPTD
160 170 180 190 200
QRNLLRAKEY LEFFRSNPMN SLPPTAFPWS GFGAPDDDLD ATKEAVAAAV
210 220 230 240 250
AAVAAGDCNG LDFYGPGPPA DRPPAGDGDE GDSTPGLWPE RDEDAPPGGL
260 270 280 290 300
FPPPTAPPAT TQNGHYGRAG AGTGEEEAAA LSEAAPEPGD SPGFLSGAAE
310 320 330 340 350
GEDGDAADVD GLAASTLLQQ MMSSVGRAGD SDEESRTDDK GVMDYYLKYF
360 370 380 390 400
SGAHEGDVYP AWSQKGEKKI RAKAFQKCPI CEKVIQGAGK LPRHIRTHTG
410 420 430 440 450
EKPYECNICK VRFTRQDKLK VHMRKHTGEK PYLCQQCGAA FAHNYDLKNH
460 470 480 490 500
MRVHTGLRPY QCDSCCKTFV RSDHLHRHLK KDGCNGVPSR RGRKPRVRGV
510 520 530 540 550
PPDVPAGAGA PPGLPDAPRN GQEKHFKDEE EDEEEASPDG SGRLNVAGSG
560
GDDGAGGPAV ATAEGNFAT
Length:569
Mass (Da):60,281
Last modified:September 2, 2008 - v2
Checksum:iB4F79FF89A88CBD5
GO
Isoform 2 (identifier: O88939-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     532-535: Missing.

Show »
Length:565
Mass (Da):59,778
Checksum:iB59CCA89D740DC70
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331L → M in BAB26897 (PubMed:16141072).Curated
Sequence conflicti548 – 5481G → D in BAE32917 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei532 – 5354Missing in isoform 2. 1 PublicationVSP_035026

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086830 mRNA. Translation: AAC35367.1.
AK010379 mRNA. Translation: BAB26897.1.
AK154907 mRNA. Translation: BAE32917.1.
CH466553 Genomic DNA. Translation: EDL31455.1.
BC057204 mRNA. Translation: AAH57204.1.
BC138524 mRNA. Translation: AAI38525.1.
BC145311 mRNA. Translation: AAI45312.1.
CCDSiCCDS35991.1. [O88939-1]
RefSeqiNP_034861.3. NM_010731.3. [O88939-1]
XP_006513343.2. XM_006513280.2. [O88939-1]
XP_006513345.1. XM_006513282.1. [O88939-1]
UniGeneiMm.20920.
Mm.486315.

Genome annotation databases

EnsembliENSMUST00000048128; ENSMUSP00000047333; ENSMUSG00000035011. [O88939-1]
ENSMUST00000117956; ENSMUSP00000113428; ENSMUSG00000035011. [O88939-1]
ENSMUST00000119606; ENSMUSP00000113612; ENSMUSG00000035011. [O88939-1]
GeneIDi16969.
KEGGimmu:16969.
UCSCiuc007gfz.1. mouse. [O88939-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086830 mRNA. Translation: AAC35367.1.
AK010379 mRNA. Translation: BAB26897.1.
AK154907 mRNA. Translation: BAE32917.1.
CH466553 Genomic DNA. Translation: EDL31455.1.
BC057204 mRNA. Translation: AAH57204.1.
BC138524 mRNA. Translation: AAI38525.1.
BC145311 mRNA. Translation: AAI45312.1.
CCDSiCCDS35991.1. [O88939-1]
RefSeqiNP_034861.3. NM_010731.3. [O88939-1]
XP_006513343.2. XM_006513280.2. [O88939-1]
XP_006513345.1. XM_006513282.1. [O88939-1]
UniGeneiMm.20920.
Mm.486315.

3D structure databases

ProteinModelPortaliO88939.
SMRiO88939. Positions 9-162, 377-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201199. 8 interactions.
IntActiO88939. 8 interactions.
MINTiMINT-4140499.
STRINGi10090.ENSMUSP00000047333.

PTM databases

iPTMnetiO88939.
PhosphoSiteiO88939.

Proteomic databases

EPDiO88939.
MaxQBiO88939.
PaxDbiO88939.
PeptideAtlasiO88939.
PRIDEiO88939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048128; ENSMUSP00000047333; ENSMUSG00000035011. [O88939-1]
ENSMUST00000117956; ENSMUSP00000113428; ENSMUSG00000035011. [O88939-1]
ENSMUST00000119606; ENSMUSP00000113612; ENSMUSG00000035011. [O88939-1]
GeneIDi16969.
KEGGimmu:16969.
UCSCiuc007gfz.1. mouse. [O88939-1]

Organism-specific databases

CTDi51341.
MGIiMGI:1335091. Zbtb7a.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129834.
HOGENOMiHOG000082521.
HOVERGENiHBG106747.
InParanoidiO88939.
KOiK10494.
OMAiQMMDSIE.
OrthoDBiEOG091G057O.
PhylomeDBiO88939.
TreeFamiTF331824.

Miscellaneous databases

PROiO88939.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035011.
CleanExiMM_ZBTB7A.
ExpressionAtlasiO88939. baseline and differential.
GenevisibleiO88939. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZBT7A_MOUSE
AccessioniPrimary (citable) accession number: O88939
Secondary accession number(s): B2RRP7
, B7ZNL6, Q3U372, Q9CRJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: September 2, 2008
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.