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O88935

- SYN1_MOUSE

UniProt

O88935 - SYN1_MOUSE

Protein

Synapsin-1

Gene

Syn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. catalytic activity Source: InterPro
    3. protein kinase binding Source: ParkinsonsUK-UCL

    GO - Biological processi

    1. neurotransmitter secretion Source: InterPro

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synapsin-1
    Alternative name(s):
    Synapsin I
    Gene namesi
    Name:Syn1
    Synonyms:Syn-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:98460. Syn1.

    Subcellular locationi

    Cell junctionsynapse 1 Publication. Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytosol Source: Ensembl
    3. dendrite Source: Ensembl
    4. Golgi apparatus Source: UniProtKB-SubCell
    5. synaptic vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Golgi apparatus, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Synapsin-1PRO_0000183019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91Phosphoserine; by CaMK1 and PKABy similarity
    Glycosylationi55 – 551O-linked (GlcNAc)By similarity
    Glycosylationi56 – 561O-linked (GlcNAc)By similarity
    Glycosylationi87 – 871O-linked (GlcNAc)1 Publication
    Glycosylationi96 – 961O-linked (GlcNAc)By similarity
    Glycosylationi103 – 1031O-linked (GlcNAc)By similarity
    Glycosylationi261 – 2611O-linked (GlcNAc)By similarity
    Modified residuei312 – 3121Phosphotyrosine1 Publication
    Modified residuei427 – 4271Phosphoserine1 Publication
    Glycosylationi432 – 4321O-linked (GlcNAc)By similarity
    Glycosylationi518 – 5181O-linked (GlcNAc)By similarity
    Glycosylationi526 – 5261O-linked (GlcNAc)1 Publication
    Modified residuei551 – 5511Phosphoserine; by PDPK1By similarity
    Modified residuei553 – 5531PhosphoserineBy similarity
    Glycosylationi564 – 5641O-linked (GlcNAc)By similarity
    Modified residuei568 – 5681Phosphoserine; by CaMK2By similarity
    Glycosylationi578 – 5781O-linked (GlcNAc)By similarity
    Modified residuei605 – 6051Phosphoserine; by CaMK2By similarity

    Post-translational modificationi

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal By similarity.By similarity
    Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiO88935.
    PaxDbiO88935.
    PRIDEiO88935.

    PTM databases

    PhosphoSiteiO88935.

    Expressioni

    Gene expression databases

    BgeeiO88935.
    CleanExiMM_SYN1.
    GenevestigatoriO88935.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 By similarity. Isoform Ib interacts with PRNP.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi203600. 7 interactions.
    IntActiO88935. 11 interactions.
    MINTiMINT-1531899.

    Structurei

    3D structure databases

    ProteinModelPortaliO88935.
    SMRiO88935. Positions 112-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2828AAdd
    BLAST
    Regioni29 – 11284B; linkerAdd
    BLAST
    Regioni113 – 420308C; actin-binding and synaptic-vesicle bindingAdd
    BLAST
    Regioni421 – 657237D; Pro-rich linkerAdd
    BLAST
    Regioni658 – 70649EAdd
    BLAST

    Domaini

    The A region binds phospholipids with a preference for negatively charged species.By similarity

    Sequence similaritiesi

    Belongs to the synapsin family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG284201.
    GeneTreeiENSGT00530000063319.
    HOGENOMiHOG000231323.
    HOVERGENiHBG016354.
    InParanoidiO88935.
    OMAiPIRQASQ.
    OrthoDBiEOG793B7G.
    PhylomeDBiO88935.
    TreeFamiTF319919.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    InterProiIPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR028713. SYN1.
    IPR001359. Synapsin.
    IPR020898. Synapsin_ATP-bd_dom.
    IPR019735. Synapsin_CS.
    IPR019736. Synapsin_P_site.
    IPR020897. Synapsin_pre-ATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR10841. PTHR10841. 1 hit.
    PTHR10841:SF8. PTHR10841:SF8. 1 hit.
    PfamiPF02078. Synapsin. 1 hit.
    PF02750. Synapsin_C. 1 hit.
    PF10581. Synapsin_N. 1 hit.
    [Graphical view]
    PRINTSiPR01368. SYNAPSIN.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    PROSITEiPS00415. SYNAPSIN_1. 1 hit.
    PS00416. SYNAPSIN_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Ia (identifier: O88935-2) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS    50
    AERASTAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG 100
    GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD 150
    LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS 200
    LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLIDQT 250
    FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 300
    YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS 350
    DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD 400
    EDKQLIVELV VNKMTQALPR QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ 450
    QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP LQQRPPPQGQ QHLSGLGPPA 500
    GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP GAPPAARPPA 550
    SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 600
    TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT 650
    AAAGGPPHPQ LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF 700
    ASLFSD 706
    Length:706
    Mass (Da):74,097
    Last modified:August 30, 2005 - v2
    Checksum:i04C940E68547372B
    GO
    Isoform Ib (identifier: O88935-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         662-670: NKSQSLTNA → KASPSQAQP
         671-706: Missing.

    Show »
    Length:670
    Mass (Da):70,035
    Checksum:iDE64BF0090A2B58E
    GO
    Isoform 3 (identifier: O88935-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         573-600: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:678
    Mass (Da):71,483
    Checksum:i3B7E5546952C5B8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441P → L in AAA79963. (PubMed:8034599)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei573 – 60028Missing in isoform 3. 1 PublicationVSP_015205Add
    BLAST
    Alternative sequencei662 – 6709NKSQSLTNA → KASPSQAQP in isoform Ib. 1 PublicationVSP_015206
    Alternative sequencei671 – 70636Missing in isoform Ib. 1 PublicationVSP_015207Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF085809 mRNA. Translation: AAD09833.1.
    BC022954 mRNA. Translation: AAH22954.1.
    L32025 Genomic DNA. Translation: AAA79963.1.
    CCDSiCCDS53017.1. [O88935-2]
    CCDS53018.1. [O88935-1]
    PIRiA53692.
    RefSeqiNP_001104250.1. NM_001110780.1. [O88935-1]
    NP_038708.3. NM_013680.4. [O88935-2]
    UniGeneiMm.439844.

    Genome annotation databases

    EnsembliENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
    ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
    GeneIDi20964.
    KEGGimmu:20964.
    UCSCiuc009stw.2. mouse. [O88935-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF085809 mRNA. Translation: AAD09833.1 .
    BC022954 mRNA. Translation: AAH22954.1 .
    L32025 Genomic DNA. Translation: AAA79963.1 .
    CCDSi CCDS53017.1. [O88935-2 ]
    CCDS53018.1. [O88935-1 ]
    PIRi A53692.
    RefSeqi NP_001104250.1. NM_001110780.1. [O88935-1 ]
    NP_038708.3. NM_013680.4. [O88935-2 ]
    UniGenei Mm.439844.

    3D structure databases

    ProteinModelPortali O88935.
    SMRi O88935. Positions 112-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203600. 7 interactions.
    IntActi O88935. 11 interactions.
    MINTi MINT-1531899.

    PTM databases

    PhosphoSitei O88935.

    Proteomic databases

    MaxQBi O88935.
    PaxDbi O88935.
    PRIDEi O88935.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081893 ; ENSMUSP00000080568 ; ENSMUSG00000037217 . [O88935-2 ]
    ENSMUST00000115345 ; ENSMUSP00000111002 ; ENSMUSG00000037217 . [O88935-1 ]
    GeneIDi 20964.
    KEGGi mmu:20964.
    UCSCi uc009stw.2. mouse. [O88935-2 ]

    Organism-specific databases

    CTDi 6853.
    MGIi MGI:98460. Syn1.

    Phylogenomic databases

    eggNOGi NOG284201.
    GeneTreei ENSGT00530000063319.
    HOGENOMi HOG000231323.
    HOVERGENi HBG016354.
    InParanoidi O88935.
    OMAi PIRQASQ.
    OrthoDBi EOG793B7G.
    PhylomeDBi O88935.
    TreeFami TF319919.

    Miscellaneous databases

    NextBioi 299922.
    PROi O88935.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88935.
    CleanExi MM_SYN1.
    Genevestigatori O88935.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    InterProi IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR028713. SYN1.
    IPR001359. Synapsin.
    IPR020898. Synapsin_ATP-bd_dom.
    IPR019735. Synapsin_CS.
    IPR019736. Synapsin_P_site.
    IPR020897. Synapsin_pre-ATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR10841. PTHR10841. 1 hit.
    PTHR10841:SF8. PTHR10841:SF8. 1 hit.
    Pfami PF02078. Synapsin. 1 hit.
    PF02750. Synapsin_C. 1 hit.
    PF10581. Synapsin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01368. SYNAPSIN.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    PROSITEi PS00415. SYNAPSIN_1. 1 hit.
    PS00416. SYNAPSIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules."
      Matsumoto K., Ebihara K., Yamamoto H., Tabuchi H., Fukunaga K., Yasunami M., Ohkubo H., Shichiri M., Miyamoto E.
      J. Biol. Chem. 274:2053-2059(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IB).
      Strain: C57BL/6.
      Tissue: Pancreatic islet.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Eye.
    3. "Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements."
      Chin L.S., Li L., Greengard P.
      J. Biol. Chem. 269:18507-18513(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
    4. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 86-108; 115-128; 177-186; 257-269; 282-311; 329-336; 414-420; 431-446 AND 566-576, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    5. "PrPC directly interacts with proteins involved in signaling pathways."
      Spielhaupter C., Schaetzl H.M.
      J. Biol. Chem. 276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry."
      Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526.
      Tissue: Brain.
    9. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiSYN1_MOUSE
    AccessioniPrimary (citable) accession number: O88935
    Secondary accession number(s): Q62279, Q8QZT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3