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Protein

Synapsin-1

Gene

Syn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. catalytic activity Source: InterPro
  3. protein kinase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  1. neurotransmitter secretion Source: InterPro
  2. regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synapsin-1
Alternative name(s):
Synapsin I
Gene namesi
Name:Syn1
Synonyms:Syn-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:98460. Syn1.

Subcellular locationi

  1. Cell junctionsynapse 1 Publication
  2. Golgi apparatus 1 Publication

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytosol Source: Ensembl
  3. dendrite Source: Ensembl
  4. Golgi apparatus Source: UniProtKB-SubCell
  5. myelin sheath Source: UniProtKB
  6. synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Golgi apparatus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Synapsin-1PRO_0000183019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine; by CaMK1 and PKABy similarity
Glycosylationi55 – 551O-linked (GlcNAc)By similarity
Glycosylationi56 – 561O-linked (GlcNAc)By similarity
Glycosylationi87 – 871O-linked (GlcNAc)1 Publication
Glycosylationi96 – 961O-linked (GlcNAc)By similarity
Glycosylationi103 – 1031O-linked (GlcNAc)By similarity
Glycosylationi261 – 2611O-linked (GlcNAc)By similarity
Modified residuei312 – 3121Phosphotyrosine1 Publication
Modified residuei427 – 4271Phosphoserine1 Publication
Glycosylationi432 – 4321O-linked (GlcNAc)By similarity
Glycosylationi518 – 5181O-linked (GlcNAc)By similarity
Glycosylationi526 – 5261O-linked (GlcNAc)1 Publication
Modified residuei551 – 5511Phosphoserine; by PDPK1By similarity
Modified residuei553 – 5531PhosphoserineBy similarity
Glycosylationi564 – 5641O-linked (GlcNAc)By similarity
Modified residuei568 – 5681Phosphoserine; by CaMK2By similarity
Glycosylationi578 – 5781O-linked (GlcNAc)By similarity
Modified residuei605 – 6051Phosphoserine; by CaMK2By similarity

Post-translational modificationi

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal (By similarity).By similarity
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO88935.
PaxDbiO88935.
PRIDEiO88935.

PTM databases

PhosphoSiteiO88935.

Expressioni

Gene expression databases

BgeeiO88935.
CleanExiMM_SYN1.
ExpressionAtlasiO88935. baseline and differential.
GenevestigatoriO88935.

Interactioni

Subunit structurei

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). Isoform Ib interacts with PRNP.By similarity1 Publication

Protein-protein interaction databases

BioGridi203600. 7 interactions.
IntActiO88935. 11 interactions.
MINTiMINT-1531899.

Structurei

3D structure databases

ProteinModelPortaliO88935.
SMRiO88935. Positions 112-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2828AAdd
BLAST
Regioni29 – 11284B; linkerAdd
BLAST
Regioni113 – 420308C; actin-binding and synaptic-vesicle bindingAdd
BLAST
Regioni421 – 657237D; Pro-rich linkerAdd
BLAST
Regioni658 – 70649EAdd
BLAST

Domaini

The A region binds phospholipids with a preference for negatively charged species.By similarity

Sequence similaritiesi

Belongs to the synapsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG284201.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiO88935.
OMAiPIRQASQ.
OrthoDBiEOG793B7G.
PhylomeDBiO88935.
TreeFamiTF319919.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PTHR10841:SF8. PTHR10841:SF8. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Ia (identifier: O88935-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS
60 70 80 90 100
AERASTAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG
110 120 130 140 150
GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD
160 170 180 190 200
LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS
210 220 230 240 250
LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLIDQT
260 270 280 290 300
FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT
310 320 330 340 350
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS
360 370 380 390 400
DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD
410 420 430 440 450
EDKQLIVELV VNKMTQALPR QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ
460 470 480 490 500
QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP LQQRPPPQGQ QHLSGLGPPA
510 520 530 540 550
GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP GAPPAARPPA
560 570 580 590 600
SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP
610 620 630 640 650
TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT
660 670 680 690 700
AAAGGPPHPQ LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF

ASLFSD
Length:706
Mass (Da):74,097
Last modified:August 30, 2005 - v2
Checksum:i04C940E68547372B
GO
Isoform Ib (identifier: O88935-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     662-670: NKSQSLTNA → KASPSQAQP
     671-706: Missing.

Show »
Length:670
Mass (Da):70,035
Checksum:iDE64BF0090A2B58E
GO
Isoform 3 (identifier: O88935-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     573-600: Missing.

Note: No experimental confirmation available.

Show »
Length:678
Mass (Da):71,483
Checksum:i3B7E5546952C5B8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441P → L in AAA79963 (PubMed:8034599).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei573 – 60028Missing in isoform 3. 1 PublicationVSP_015205Add
BLAST
Alternative sequencei662 – 6709NKSQSLTNA → KASPSQAQP in isoform Ib. 1 PublicationVSP_015206
Alternative sequencei671 – 70636Missing in isoform Ib. 1 PublicationVSP_015207Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085809 mRNA. Translation: AAD09833.1.
BC022954 mRNA. Translation: AAH22954.1.
L32025 Genomic DNA. Translation: AAA79963.1.
CCDSiCCDS53017.1. [O88935-2]
CCDS53018.1. [O88935-1]
PIRiA53692.
RefSeqiNP_001104250.1. NM_001110780.1. [O88935-1]
NP_038708.3. NM_013680.4. [O88935-2]
UniGeneiMm.439844.

Genome annotation databases

EnsembliENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
GeneIDi20964.
KEGGimmu:20964.
UCSCiuc009stw.2. mouse. [O88935-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085809 mRNA. Translation: AAD09833.1.
BC022954 mRNA. Translation: AAH22954.1.
L32025 Genomic DNA. Translation: AAA79963.1.
CCDSiCCDS53017.1. [O88935-2]
CCDS53018.1. [O88935-1]
PIRiA53692.
RefSeqiNP_001104250.1. NM_001110780.1. [O88935-1]
NP_038708.3. NM_013680.4. [O88935-2]
UniGeneiMm.439844.

3D structure databases

ProteinModelPortaliO88935.
SMRiO88935. Positions 112-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203600. 7 interactions.
IntActiO88935. 11 interactions.
MINTiMINT-1531899.

PTM databases

PhosphoSiteiO88935.

Proteomic databases

MaxQBiO88935.
PaxDbiO88935.
PRIDEiO88935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
GeneIDi20964.
KEGGimmu:20964.
UCSCiuc009stw.2. mouse. [O88935-2]

Organism-specific databases

CTDi6853.
MGIiMGI:98460. Syn1.

Phylogenomic databases

eggNOGiNOG284201.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiO88935.
OMAiPIRQASQ.
OrthoDBiEOG793B7G.
PhylomeDBiO88935.
TreeFamiTF319919.

Miscellaneous databases

NextBioi299922.
PROiO88935.
SOURCEiSearch...

Gene expression databases

BgeeiO88935.
CleanExiMM_SYN1.
ExpressionAtlasiO88935. baseline and differential.
GenevestigatoriO88935.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PTHR10841:SF8. PTHR10841:SF8. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules."
    Matsumoto K., Ebihara K., Yamamoto H., Tabuchi H., Fukunaga K., Yasunami M., Ohkubo H., Shichiri M., Miyamoto E.
    J. Biol. Chem. 274:2053-2059(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IB).
    Strain: C57BL/6.
    Tissue: Pancreatic islet.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Eye.
  3. "Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements."
    Chin L.S., Li L., Greengard P.
    J. Biol. Chem. 269:18507-18513(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
  4. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 86-108; 115-128; 177-186; 257-269; 282-311; 329-336; 414-420; 431-446 AND 566-576, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  5. "PrPC directly interacts with proteins involved in signaling pathways."
    Spielhaupter C., Schaetzl H.M.
    J. Biol. Chem. 276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry."
    Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526.
    Tissue: Brain.
  9. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiSYN1_MOUSE
AccessioniPrimary (citable) accession number: O88935
Secondary accession number(s): Q62279, Q8QZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: March 4, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.