Synapsin-1 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Synapsin-1

Alternative name(s):
Synapsin I

Gene names

Name:	Syn1
Synonyms:	Syn-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.
Subunit structure	Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 By similarity. Isoform Ib interacts with PRNP. Ref.5
Subcellular location	Cell junction › synapse. Golgi apparatus Ref.5.
Domain	The A region binds phospholipids with a preference for negatively charged species By similarity.
Post-translational modification	Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal By similarity. Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles By similarity.
Sequence similarities	Belongs to the synapsin family.

Ontologies

Keywords
Cellular component	Cell junction Golgi apparatus Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Actin-binding
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC neurotransmitter secretion Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara synaptic vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Ia (identifier: O88935-2) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Ib (identifier: O88935-1) The sequence of this isoform differs from the canonical sequence as follows: 662-670: NKSQSLTNA → KASPSQAQP 671-706: Missing.

Isoform 3 (identifier: O88935-3) The sequence of this isoform differs from the canonical sequence as follows: 573-600: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 706

706

Synapsin-1

PRO_0000183019

Regions

Region

1 – 28

28

A

Region

29 – 112

84

B; linker

Region

113 – 420

308

C; actin-binding and synaptic-vesicle binding

Region

421 – 657

237

D; Pro-rich linker

Region

658 – 706

49

E

Amino acid modifications

Modified residue

9

1

Phosphoserine; by CaMK1 and PKA; alternate By similarity

Modified residue

9

1

Phosphoserine; by PKA and CaMK1; alternate By similarity

Modified residue

62

1

Phosphoserine Ref.10

Modified residue

67

1

Phosphoserine Ref.10

Modified residue

312

1

Phosphotyrosine Ref.9

Modified residue

337

1

Phosphothreonine Ref.10

Modified residue

427

1

Phosphoserine Ref.7 Ref.10

Modified residue

510

1

Phosphoserine Ref.6

Modified residue

512

1

Phosphothreonine Ref.10

Modified residue

520

1

Phosphoserine Ref.10

Modified residue

551

1

Phosphoserine; by PDPK1 By similarity

Modified residue

553

1

Phosphoserine Ref.10

Modified residue

568

1

Phosphoserine Ref.7

Modified residue

605

1

Phosphoserine; by CaMK2 By similarity

Modified residue

666

1

Phosphoserine Ref.10

Modified residue

705

1

Phosphoserine Ref.10

Glycosylation

55

1

O-linked (GlcNAc) By similarity

Glycosylation

56

1

O-linked (GlcNAc) By similarity

Glycosylation

87

1

O-linked (GlcNAc) Ref.8

Glycosylation

96

1

O-linked (GlcNAc) By similarity

Glycosylation

103

1

O-linked (GlcNAc) By similarity

Glycosylation

261

1

O-linked (GlcNAc) By similarity

Glycosylation

432

1

O-linked (GlcNAc) By similarity

Glycosylation

518

1

O-linked (GlcNAc) By similarity

Glycosylation

526

1

O-linked (GlcNAc) Ref.8

Glycosylation

564

1

O-linked (GlcNAc) By similarity

Glycosylation

578

1

O-linked (GlcNAc) By similarity

Natural variations

Alternative sequence

573 – 600

28

Missing in isoform 3.

VSP_015205

Alternative sequence

662 – 670

9

NKSQSLTNA → KASPSQAQP in isoform Ib.

VSP_015206

Alternative sequence

671 – 706

36

Missing in isoform Ib.

VSP_015207

Experimental info

Sequence conflict

44

1

P → L in AAA79963. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform Ia [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 04C940E68547372B Show »	FASTA	706	74,097
10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT AAAGGPPHPQ 670 680 690 700 LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF ASLFSD « Hide
Isoform Ib [UniParc]. Checksum: DE64BF0090A2B58E Show »	FASTA	670	70,035
10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT AAAGGPPHPQ 670 LKASPSQAQP « Hide
Isoform 3 [UniParc]. Checksum: 3B7E5546952C5B8F Show »	FASTA	678	71,483
10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPTRQASQAG PGPRTGPPTT QQPRPSGPGP 610 620 630 640 650 660 AGRPAKPQLA QKPSQDVPPP ITAAAGGPPH PQLNKSQSLT NAFNLPEPAP PRPSLSQDEV 670 KAETIRSLRK SFASLFSD « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning from insulinoma cells of synapsin I associated with insulin secretory granules." Matsumoto K., Ebihara K., Yamamoto H., Tabuchi H., Fukunaga K., Yasunami M., Ohkubo H., Shichiri M., Miyamoto E. J. Biol. Chem. 274:2053-2059(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IB). Strain: C57BL/6. Tissue: Pancreatic islet.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Eye.
[3]	"Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements." Chin L.S., Li L., Greengard P. J. Biol. Chem. 269:18507-18513(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
[4]	Lubec G., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 86-108; 115-128; 177-186; 257-269; 282-311; 329-336; 414-420; 431-446 AND 566-576, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[5]	"PrPC directly interacts with proteins involved in signaling pathways." Spielhaupter C., Schaetzl H.M. J. Biol. Chem. 276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
[6]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510, MASS SPECTROMETRY. Tissue: Embryonic brain.
[7]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427 AND SER-568, MASS SPECTROMETRY. Tissue: Brain.
[8]	"O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry." Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526, MASS SPECTROMETRY. Tissue: Brain.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-67; THR-337; SER-427; THR-512; SER-520; SER-553; SER-666 AND SER-705, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085809 mRNA. Translation: AAD09833.1. BC022954 mRNA. Translation: AAH22954.1. L32025 Genomic DNA. Translation: AAA79963.1.
IPI	IPI00136372. IPI00649467. IPI00649886.
PIR	A53692.
RefSeq	NP_001104250.1. NM_001110780.1. NP_038708.3. NM_013680.4.
UniGene	Mm.439844.
3D structure databases
ProteinModelPortal	O88935.
SMR	O88935. Positions 112-417.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88935. 10 interactions.
MINT	MINT-1531899.
PTM databases
PhosphoSite	O88935.
Proteomic databases
PaxDb	O88935.
PRIDE	O88935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217.
GeneID	20964.
KEGG	mmu:20964.
UCSC	uc009stw.2. mouse.
Organism-specific databases
CTD	6853.
MGI	MGI:98460. Syn1.
Phylogenomic databases
eggNOG	NOG284201.
GeneTree	ENSGT00530000063319.
HOGENOM	HOG000231323.
HOVERGEN	HBG016354.
InParanoid	O88935.
Gene expression databases
ArrayExpress	O88935.
Bgee	O88935.
CleanEx	MM_SYN1.
Genevestigator	O88935.
GermOnline	ENSMUSG00000037217. Mus musculus.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR001359. Synapsin. IPR020898. Synapsin_ATP-bd_dom. IPR019735. Synapsin_CS. IPR019736. Synapsin_P_site. IPR020897. Synapsin_pre-ATP-grasp_dom. [Graphical view]
Pfam	PF02078. Synapsin. 1 hit. PF02750. Synapsin_C. 1 hit. PF10581. Synapsin_N. 1 hit. [Graphical view]
PRINTS	PR01368. SYNAPSIN.
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS00415. SYNAPSIN_1. 1 hit. PS00416. SYNAPSIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	299922.
SOURCE	Search...

Entry information

Entry name

SYN1_MOUSE

Accession

Primary (citable) accession number: O88935
Secondary accession number(s): Q62279, Q8QZT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 30, 2005
Last modified:	April 3, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents