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Protein

Synapsin-1

Gene

Syn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • catalytic activity Source: InterPro
  • protein dimerization activity Source: MGI
  • protein kinase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • neurotransmitter secretion Source: MGI
  • regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synapsin-1
Alternative name(s):
Synapsin I
Gene namesi
Name:Syn1
Synonyms:Syn-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:98460. Syn1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • cytosol Source: Ensembl
  • dendrite Source: Ensembl
  • Golgi apparatus Source: UniProtKB-SubCell
  • myelin sheath Source: UniProtKB
  • postsynaptic density Source: MGI
  • presynaptic active zone Source: MGI
  • synapse Source: MGI
  • synaptic vesicle membrane Source: MGI
  • synaptonemal complex Source: MGI
  • terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Golgi apparatus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830191 – 706Synapsin-1Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Glycosylationi55O-linked (GlcNAc)By similarity1
Glycosylationi56O-linked (GlcNAc)By similarity1
Modified residuei62PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Glycosylationi87O-linked (GlcNAc)1 Publication1
Glycosylationi96O-linked (GlcNAc)By similarity1
Glycosylationi103O-linked (GlcNAc)By similarity1
Glycosylationi261O-linked (GlcNAc)By similarity1
Modified residuei312PhosphotyrosineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei430Omega-N-methylarginineCombined sources1
Modified residuei432Phosphoserine; alternateCombined sources1
Glycosylationi432O-linked (GlcNAc); alternateBy similarity1
Modified residuei434PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei438PhosphoserineCombined sources1
Modified residuei476Omega-N-methylarginineCombined sources1
Glycosylationi518O-linked (GlcNAc)By similarity1
Glycosylationi526O-linked (GlcNAc)1 Publication1
Modified residuei534Omega-N-methylarginineCombined sources1
Modified residuei547Omega-N-methylarginineCombined sources1
Modified residuei551Phosphoserine; by PDPK1By similarity1
Modified residuei553PhosphoserineBy similarity1
Modified residuei556Omega-N-methylarginineCombined sources1
Glycosylationi564O-linked (GlcNAc)By similarity1
Modified residuei568Phosphoserine; by CaMK2By similarity1
Glycosylationi578O-linked (GlcNAc)By similarity1
Modified residuei605Phosphoserine; by CaMK2By similarity1
Modified residuei622Omega-N-methylarginineCombined sources1
Modified residuei664PhosphoserineCombined sources1
Modified residuei666PhosphoserineCombined sources1
Modified residuei680Asymmetric dimethylarginineCombined sources1
Modified residuei684PhosphoserineCombined sources1

Post-translational modificationi

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal (By similarity).By similarity
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiO88935.
PeptideAtlasiO88935.
PRIDEiO88935.

PTM databases

iPTMnetiO88935.
PhosphoSitePlusiO88935.
SwissPalmiO88935.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037217.
CleanExiMM_SYN1.
GenevisibleiO88935. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). Isoform Ib interacts with PRNP.By similarity1 Publication

GO - Molecular functioni

  • protein dimerization activity Source: MGI
  • protein kinase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi203600. 9 interactors.
IntActiO88935. 14 interactors.
MINTiMINT-1531899.
STRINGi10090.ENSMUSP00000080568.

Structurei

3D structure databases

ProteinModelPortaliO88935.
SMRiO88935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 28AAdd BLAST28
Regioni29 – 112B; linkerAdd BLAST84
Regioni113 – 420C; actin-binding and synaptic-vesicle bindingAdd BLAST308
Regioni421 – 657D; Pro-rich linkerAdd BLAST237
Regioni658 – 706EAdd BLAST49

Domaini

The A region binds phospholipids with a preference for negatively charged species.By similarity

Sequence similaritiesi

Belongs to the synapsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiO88935.
KOiK19941.
OMAiPIRQASQ.
OrthoDBiEOG091G0BZ1.
PhylomeDBiO88935.
TreeFamiTF319919.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 2 hits.
PTHR10841:SF22. PTHR10841:SF22. 2 hits.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Ia (identifier: O88935-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS
60 70 80 90 100
AERASTAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG
110 120 130 140 150
GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD
160 170 180 190 200
LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS
210 220 230 240 250
LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLIDQT
260 270 280 290 300
FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT
310 320 330 340 350
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS
360 370 380 390 400
DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD
410 420 430 440 450
EDKQLIVELV VNKMTQALPR QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ
460 470 480 490 500
QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP LQQRPPPQGQ QHLSGLGPPA
510 520 530 540 550
GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP GAPPAARPPA
560 570 580 590 600
SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP
610 620 630 640 650
TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT
660 670 680 690 700
AAAGGPPHPQ LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF

ASLFSD
Length:706
Mass (Da):74,097
Last modified:August 30, 2005 - v2
Checksum:i04C940E68547372B
GO
Isoform Ib (identifier: O88935-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     662-670: NKSQSLTNA → KASPSQAQP
     671-706: Missing.

Show »
Length:670
Mass (Da):70,035
Checksum:iDE64BF0090A2B58E
GO
Isoform 3 (identifier: O88935-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     573-600: Missing.

Note: No experimental confirmation available.
Show »
Length:678
Mass (Da):71,483
Checksum:i3B7E5546952C5B8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44P → L in AAA79963 (PubMed:8034599).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015205573 – 600Missing in isoform 3. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_015206662 – 670NKSQSLTNA → KASPSQAQP in isoform Ib. 1 Publication9
Alternative sequenceiVSP_015207671 – 706Missing in isoform Ib. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085809 mRNA. Translation: AAD09833.1.
BC022954 mRNA. Translation: AAH22954.1.
L32025 Genomic DNA. Translation: AAA79963.1.
CCDSiCCDS53017.1. [O88935-2]
CCDS53018.1. [O88935-1]
PIRiA53692.
RefSeqiNP_001104250.1. NM_001110780.1. [O88935-1]
NP_038708.3. NM_013680.4. [O88935-2]
UniGeneiMm.439844.

Genome annotation databases

EnsembliENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
GeneIDi20964.
KEGGimmu:20964.
UCSCiuc009stw.2. mouse. [O88935-2]
uc009stx.2. mouse. [O88935-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085809 mRNA. Translation: AAD09833.1.
BC022954 mRNA. Translation: AAH22954.1.
L32025 Genomic DNA. Translation: AAA79963.1.
CCDSiCCDS53017.1. [O88935-2]
CCDS53018.1. [O88935-1]
PIRiA53692.
RefSeqiNP_001104250.1. NM_001110780.1. [O88935-1]
NP_038708.3. NM_013680.4. [O88935-2]
UniGeneiMm.439844.

3D structure databases

ProteinModelPortaliO88935.
SMRiO88935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203600. 9 interactors.
IntActiO88935. 14 interactors.
MINTiMINT-1531899.
STRINGi10090.ENSMUSP00000080568.

PTM databases

iPTMnetiO88935.
PhosphoSitePlusiO88935.
SwissPalmiO88935.

Proteomic databases

PaxDbiO88935.
PeptideAtlasiO88935.
PRIDEiO88935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
GeneIDi20964.
KEGGimmu:20964.
UCSCiuc009stw.2. mouse. [O88935-2]
uc009stx.2. mouse. [O88935-1]

Organism-specific databases

CTDi6853.
MGIiMGI:98460. Syn1.

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiO88935.
KOiK19941.
OMAiPIRQASQ.
OrthoDBiEOG091G0BZ1.
PhylomeDBiO88935.
TreeFamiTF319919.

Miscellaneous databases

PROiO88935.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037217.
CleanExiMM_SYN1.
GenevisibleiO88935. MM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 2 hits.
PTHR10841:SF22. PTHR10841:SF22. 2 hits.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN1_MOUSE
AccessioniPrimary (citable) accession number: O88935
Secondary accession number(s): Q62279, Q8QZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.