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O88935 (SYN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synapsin-1
Alternative name(s):
Synapsin I
Gene names
Name:Syn1
Synonyms:Syn-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

Subunit structure

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 By similarity. Isoform Ib interacts with PRNP. Ref.5

Subcellular location

Cell junctionsynapse. Golgi apparatus Ref.5.

Domain

The A region binds phospholipids with a preference for negatively charged species By similarity.

Post-translational modification

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal By similarity.

Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles By similarity.

Sequence similarities

Belongs to the synapsin family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Ia (identifier: O88935-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Ib (identifier: O88935-1)

The sequence of this isoform differs from the canonical sequence as follows:
     662-670: NKSQSLTNA → KASPSQAQP
     671-706: Missing.
Isoform 3 (identifier: O88935-3)

The sequence of this isoform differs from the canonical sequence as follows:
     573-600: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Synapsin-1
PRO_0000183019

Regions

Region1 – 2828A
Region29 – 11284B; linker
Region113 – 420308C; actin-binding and synaptic-vesicle binding
Region421 – 657237D; Pro-rich linker
Region658 – 70649E

Amino acid modifications

Modified residue91Phosphoserine; by CaMK1 and PKA; alternate By similarity
Modified residue91Phosphoserine; by PKA and CaMK1; alternate By similarity
Modified residue3121Phosphotyrosine Ref.9
Modified residue4271Phosphoserine Ref.7
Modified residue5511Phosphoserine; by PDPK1 By similarity
Modified residue5531Phosphoserine By similarity
Modified residue5681Phosphoserine By similarity
Modified residue6051Phosphoserine; by CaMK2 By similarity
Glycosylation551O-linked (GlcNAc) By similarity
Glycosylation561O-linked (GlcNAc) By similarity
Glycosylation871O-linked (GlcNAc) Ref.8
Glycosylation961O-linked (GlcNAc) By similarity
Glycosylation1031O-linked (GlcNAc) By similarity
Glycosylation2611O-linked (GlcNAc) By similarity
Glycosylation4321O-linked (GlcNAc) By similarity
Glycosylation5181O-linked (GlcNAc) By similarity
Glycosylation5261O-linked (GlcNAc) Ref.8
Glycosylation5641O-linked (GlcNAc) By similarity
Glycosylation5781O-linked (GlcNAc) By similarity

Natural variations

Alternative sequence573 – 60028Missing in isoform 3.
VSP_015205
Alternative sequence662 – 6709NKSQSLTNA → KASPSQAQP in isoform Ib.
VSP_015206
Alternative sequence671 – 70636Missing in isoform Ib.
VSP_015207

Experimental info

Sequence conflict441P → L in AAA79963. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Ia [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 04C940E68547372B

FASTA70674,097
        10         20         30         40         50         60 
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 

        70         80         90        100        110        120 
ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 

       130        140        150        160        170        180 
EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 

       190        200        210        220        230        240 
DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 

       250        260        270        280        290        300 
TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 

       310        320        330        340        350        360 
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 

       370        380        390        400        410        420 
SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 

       430        440        450        460        470        480 
QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 

       490        500        510        520        530        540 
LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 

       550        560        570        580        590        600 
GAPPAARPPA SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 

       610        620        630        640        650        660 
TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT AAAGGPPHPQ 

       670        680        690        700 
LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF ASLFSD 

« Hide

Isoform Ib [UniParc].

Checksum: DE64BF0090A2B58E
Show »

FASTA67070,035
Isoform 3 [UniParc].

Checksum: 3B7E5546952C5B8F
Show »

FASTA67871,483

References

« Hide 'large scale' references
[1]"Cloning from insulinoma cells of synapsin I associated with insulin secretory granules."
Matsumoto K., Ebihara K., Yamamoto H., Tabuchi H., Fukunaga K., Yasunami M., Ohkubo H., Shichiri M., Miyamoto E.
J. Biol. Chem. 274:2053-2059(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IB).
Strain: C57BL/6.
Tissue: Pancreatic islet.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Eye.
[3]"Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements."
Chin L.S., Li L., Greengard P.
J. Biol. Chem. 269:18507-18513(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
[4]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 86-108; 115-128; 177-186; 257-269; 282-311; 329-336; 414-420; 431-446 AND 566-576, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[5]"PrPC directly interacts with proteins involved in signaling pathways."
Spielhaupter C., Schaetzl H.M.
J. Biol. Chem. 276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
[6]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[7]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[8]"O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry."
Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526.
Tissue: Brain.
[9]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF085809 mRNA. Translation: AAD09833.1.
BC022954 mRNA. Translation: AAH22954.1.
L32025 Genomic DNA. Translation: AAA79963.1.
PIRA53692.
RefSeqNP_001104250.1. NM_001110780.1.
NP_038708.3. NM_013680.4.
UniGeneMm.439844.

3D structure databases

ProteinModelPortalO88935.
SMRO88935. Positions 112-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203600. 7 interactions.
IntActO88935. 11 interactions.
MINTMINT-1531899.

PTM databases

PhosphoSiteO88935.

Proteomic databases

PaxDbO88935.
PRIDEO88935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. [O88935-2]
ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217. [O88935-1]
GeneID20964.
KEGGmmu:20964.
UCSCuc009stw.2. mouse. [O88935-2]

Organism-specific databases

CTD6853.
MGIMGI:98460. Syn1.

Phylogenomic databases

eggNOGNOG284201.
GeneTreeENSGT00530000063319.
HOGENOMHOG000231323.
HOVERGENHBG016354.
InParanoidO88935.
OMAPIRQASQ.
OrthoDBEOG793B7G.
PhylomeDBO88935.
TreeFamTF319919.

Gene expression databases

BgeeO88935.
CleanExMM_SYN1.
GenevestigatorO88935.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERPTHR10841:SF8. PTHR10841:SF8. 1 hit.
PfamPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSPR01368. SYNAPSIN.
SUPFAMSSF52440. SSF52440. 1 hit.
PROSITEPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio299922.
PROO88935.
SOURCESearch...

Entry information

Entry nameSYN1_MOUSE
AccessionPrimary (citable) accession number: O88935
Secondary accession number(s): Q62279, Q8QZT8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot