O88935 (SYN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synapsin-1

Alternative name(s):
Synapsin I

Gene names

Name:	Syn1
Synonyms:	Syn-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.
Subunit structure	Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 By similarity. Isoform Ib interacts with PRNP. Ref.5
Subcellular location	Cell junction › synapse. Golgi apparatus Ref.5.
Post-translational modification	Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal By similarity.
Sequence similarities	Belongs to the synapsin family.

Ontologies

Keywords
Cellular component	Cell junction Golgi apparatus Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Actin-binding
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	neurotransmitter secretion Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW presynaptic active zone Inferred from direct assay. Source: MGI synaptic vesicle membrane Inferred from direct assay. Source: MGI synaptonemal complex Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro actin binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.5. Source: MGI protein dimerization activity Traceable author statement. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Ia (identifier: O88935-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Ib (identifier: O88935-1)

The sequence of this isoform differs from the canonical sequence as follows:
     662-670: NKSQSLTNA → KASPSQAQP
     671-706: Missing.

Isoform 3 (identifier: O88935-3)

The sequence of this isoform differs from the canonical sequence as follows:
     573-600: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Synapsin-1		PRO_0000183019
Regions
	Region	1 – 28	28	A
	Region	29 – 112	84	B; linker
	Region	113 – 420	308	C; actin-binding and synaptic-vesicle binding
	Region	421 – 657	237	D; Pro-rich linker
	Region	658 – 706	49	E
Amino acid modifications
	Modified residue	9	1	Phosphoserine; by CaMK1 and PKA By similarity
	Modified residue	39	1	Phosphoserine By similarity
	Modified residue	62	1	Phosphoserine Ref.10
	Modified residue	67	1	Phosphoserine Ref.10
	Modified residue	312	1	Phosphotyrosine Ref.9
	Modified residue	337	1	Phosphothreonine Ref.10
	Modified residue	427	1	Phosphoserine Ref.7 Ref.10
	Modified residue	510	1	Phosphoserine Ref.6
	Modified residue	512	1	Phosphothreonine Ref.10
	Modified residue	520	1	Phosphoserine Ref.10
	Modified residue	551	1	Phosphoserine; by PDPK1 By similarity
	Modified residue	553	1	Phosphoserine Ref.10
	Modified residue	568	1	Phosphoserine Ref.7
	Modified residue	605	1	Phosphoserine; by CaMK2 By similarity
	Modified residue	666	1	Phosphoserine Ref.10
	Modified residue	705	1	Phosphoserine Ref.10
	Glycosylation	55	1	O-linked (GlcNAc) By similarity
	Glycosylation	56	1	O-linked (GlcNAc) By similarity
	Glycosylation	87	1	O-linked (GlcNAc) Ref.8
	Glycosylation	96	1	O-linked (GlcNAc) By similarity
	Glycosylation	103	1	O-linked (GlcNAc) By similarity
	Glycosylation	261	1	O-linked (GlcNAc) By similarity
	Glycosylation	432	1	O-linked (GlcNAc) By similarity
	Glycosylation	518	1	O-linked (GlcNAc) By similarity
	Glycosylation	526	1	O-linked (GlcNAc) Ref.8
	Glycosylation	564	1	O-linked (GlcNAc) By similarity
	Glycosylation	578	1	O-linked (GlcNAc) By similarity
Natural variations
	Alternative sequence	573 – 600	28	Missing in isoform 3.		VSP_015205
	Alternative sequence	662 – 670	9	NKSQSLTNA → KASPSQAQP in isoform Ib.		VSP_015206
	Alternative sequence	671 – 706	36	Missing in isoform Ib.		VSP_015207
Experimental info
	Sequence conflict	44	1	P → L in AAA79963. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Ia [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 04C940E68547372B	FASTA	706	74,097
	10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT AAAGGPPHPQ 670 680 690 700 LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF ASLFSD
	Isoform Ib [UniParc]. Checksum: DE64BF0090A2B58E	FASTA	670	70,035
	10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT AAAGGPPHPQ 670 LKASPSQAQP
	Isoform 3 [UniParc]. Checksum: 3B7E5546952C5B8F	FASTA	678	71,483
	10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS AERASTAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 430 440 450 460 470 480 QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPPPQGQ QHLSGLGPPA GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPAARPPA SPSPQRQAGA PQATRQASIS GPTRQASQAG PGPRTGPPTT QQPRPSGPGP 610 620 630 640 650 660 AGRPAKPQLA QKPSQDVPPP ITAAAGGPPH PQLNKSQSLT NAFNLPEPAP PRPSLSQDEV 670 KAETIRSLRK SFASLFSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning from insulinoma cells of synapsin I associated with insulin secretory granules." Matsumoto K., Ebihara K., Yamamoto H., Tabuchi H., Fukunaga K., Yasunami M., Ohkubo H., Shichiri M., Miyamoto E. J. Biol. Chem. 274:2053-2059(1999) [PubMed: 9890964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IB). Strain: C57BL/6. Tissue: Pancreatic islet.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Eye.
[3]	"Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements." Chin L.S., Li L., Greengard P. J. Biol. Chem. 269:18507-18513(1994) [PubMed: 8034599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
[4]	Lubec G., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 86-108; 115-128; 177-186; 257-269; 282-311; 329-336; 414-420; 431-446 AND 566-576, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[5]	"PrPC directly interacts with proteins involved in signaling pathways." Spielhaupter C., Schaetzl H.M. J. Biol. Chem. 276:44604-44612(2001) [PubMed: 11571277] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PRNP.
[6]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510, MASS SPECTROMETRY. Tissue: Embryonic brain.
[7]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427 AND SER-568, MASS SPECTROMETRY. Tissue: Brain.
[8]	"O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry." Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:923-934(2006) [PubMed: 16452088] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-87 AND THR-526, MASS SPECTROMETRY. Tissue: Brain.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-312, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-67; THR-337; SER-427; THR-512; SER-520; SER-553; SER-666 AND SER-705, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085809 mRNA. Translation: AAD09833.1. BC022954 mRNA. Translation: AAH22954.1. L32025 Genomic DNA. Translation: AAA79963.1.
IPI	IPI00136372. IPI00649467. IPI00649886.
PIR	A53692.
RefSeq	NP_001104250.1. NM_001110780.1. NP_038708.3. NM_013680.4.
UniGene	Mm.439844.
3D structure databases
ProteinModelPortal	O88935.
SMR	O88935. Positions 112-417.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88935. 9 interactions.
MINT	MINT-1531899.
STRING	O88935.
PTM databases
PhosphoSite	O88935.
Proteomic databases
PRIDE	O88935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041344; ENSMUSP00000037545; ENSMUSG00000037217. ENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217. ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217.
GeneID	20964.
KEGG	mmu:20964.
UCSC	uc009stw.2. mouse.
Organism-specific databases
CTD	6853.
MGI	MGI:98460. Syn1.
Phylogenomic databases
GeneTree	ENSGT00530000063319.
HOGENOM	HBG445598.
HOVERGEN	HBG016354.
InParanoid	O88935.
OMA	RNGVKVM.
Gene expression databases
ArrayExpress	O88935.
Bgee	O88935.
CleanEx	MM_SYN1.
Genevestigator	O88935.
GermOnline	ENSMUSG00000037217. Mus musculus.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR001359. Synapsin. IPR020898. Synapsin_ATP-bd_dom. IPR019735. Synapsin_CS. IPR019736. Synapsin_P_site. IPR020897. Synapsin_pre-ATP-grasp_dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02078. Synapsin. 1 hit. PF02750. Synapsin_C. 1 hit. PF10581. Synapsin_N. 1 hit. [Graphical view]
PRINTS	PR01368. SYNAPSIN.
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS00415. SYNAPSIN_1. 1 hit. PS00416. SYNAPSIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	299922.
SOURCE	Search...

Entry information

Entry name

SYN1_MOUSE

Accession

Primary (citable) accession number: O88935
Secondary accession number(s): Q62279, Q8QZT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program