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Protein

Adhesion G protein-coupled receptor L2

Gene

Adgrl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor probably implicated in the regulation of exocytosis.1 Publication

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • G-protein coupled receptor activity Source: RGD
  • PDZ domain binding Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • cell surface receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Lectin

Protein family/group databases

MEROPSiP02.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor L2
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 2
Short name:
CIRL-2
Latrophilin-2
Gene namesi
Name:Adgrl2
Synonyms:Cirl2, Cl2, Lphn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620835. Adgrl2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 855ExtracellularCuratedAdd BLAST830
Transmembranei856 – 876Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini877 – 884CytoplasmicCurated8
Transmembranei885 – 905Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini906 – 911ExtracellularCurated6
Transmembranei912 – 932Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini933 – 956CytoplasmicCuratedAdd BLAST24
Transmembranei957 – 977Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini978 – 994ExtracellularCuratedAdd BLAST17
Transmembranei995 – 1015Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1016 – 1065CytoplasmicCuratedAdd BLAST50
Transmembranei1066 – 1086Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1087 – 1090ExtracellularCurated4
Transmembranei1091 – 1111Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1112 – 1487CytoplasmicCuratedAdd BLAST376

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • neuron projection Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi207.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001291126 – 1487Adhesion G protein-coupled receptor L2Add BLAST1462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi140 ↔ 322PROSITE-ProRule annotation
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1
Glycosylationi524N-linked (GlcNAc...)Sequence analysis1
Glycosylationi633N-linked (GlcNAc...)Sequence analysis1
Glycosylationi735N-linked (GlcNAc...)Sequence analysis1
Glycosylationi748N-linked (GlcNAc...)Sequence analysis1
Glycosylationi791N-linked (GlcNAc...)Sequence analysis1
Glycosylationi796N-linked (GlcNAc...)Sequence analysis1
Glycosylationi817N-linked (GlcNAc...)Sequence analysis1
Modified residuei1402PhosphoserineBy similarity1
Modified residuei1437PhosphoserineBy similarity1
Modified residuei1458PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei828 – 829CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88923.
PRIDEiO88923.

PTM databases

iPTMnetiO88923.
PhosphoSitePlusiO88923.

Expressioni

Tissue specificityi

Expressed ubiquitously with highest concentrations found in placenta, kidney, spleen, ovary, heart and lung.1 Publication

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region non-covalently linked to a seven-transmembrane moiety.

GO - Molecular functioni

  • PDZ domain binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042136.

Structurei

3D structure databases

ProteinModelPortaliO88923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 130SUEL-type lectinPROSITE-ProRule annotationAdd BLAST90
Domaini139 – 398Olfactomedin-likePROSITE-ProRule annotationAdd BLAST260
Domaini789 – 840GPSPROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 1 olfactomedin-like domain.PROSITE-ProRule annotation
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3545. Eukaryota.
KOG4193. Eukaryota.
KOG4729. Eukaryota.
ENOG410XSD2. LUCA.
HOVERGENiHBG052337.
InParanoidiO88923.
KOiK04593.
PhylomeDBiO88923.

Family and domain databases

InterProiIPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR031240. Latrophilin-2.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like_dom.
[Graphical view]
PANTHERiPTHR12011:SF61. PTHR12011:SF61. 3 hits.
PfamiPF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTiSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88923-7) [UniParc]FASTAAdd to basket
Also known as: CL2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSSGCRMRS LWFIMIISFS PNTEGFSRAA LPFGLVRREL SCEGYSIDLR
60 70 80 90 100
CPGSDVIMIE SANYGRTDDK ICDADPFQME NTDCYLPDAF KIMTQRCNNR
110 120 130 140 150
TQCVVVTGSD VFPDPCPGTY KYLEVQYECV PYMEQKVFVC PGTLKAIVDS
160 170 180 190 200
PSIYEAEQKA GAWCKDPLQA ADKIYFMPWT PYRTDTLIEY ASLEDFQNSR
210 220 230 240 250
QTTTYKLPNR VDGTGFVVYD GAVFFNKERT RNIVKFDLRT RIKSGEAIIN
260 270 280 290 300
YANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEQNNGMI VISQLNPYTL
310 320 330 340 350
RFEATWETTY DKRAASNAFM ICGVLYVVRS VYQDNESEAG KNVIDYIYNT
360 370 380 390 400
RLSRGEHVDV PFPNQYQYIA AVDYNPRDNQ LYVWNNNFIL RYSLEFGPPD
410 420 430 440 450
PAQVPTTAVT ITSSAELFKT TVSTTSSTSQ RGPVSSTVAG PQEGSRGTKP
460 470 480 490 500
PPAVSTTKIP PVTNIFPLPE RFCEALEMKG IKWPQTQRGM MVERPCPKGT
510 520 530 540 550
RGTASYLCMA STGTWNPKGP DLSNCTSHWV NQLAQKIRSG ENAASLANEL
560 570 580 590 600
AKHTKGTVFA GDVSSSVRLM EQLVDILDAQ LQELKPSEKD SAGRSYNKLQ
610 620 630 640 650
KREKTCRAYL KAIVDTVDNL LRAETLDCWK HMNSSEQAHT ATMLLDTLEE
660 670 680 690 700
GAFVLADNLL EPTRVSMPTD NIVLEVAVLS TEGQVQDFTF HLGFKGAFSS
710 720 730 740 750
IQLSANTVKQ NSRNGLAKVV FIIYRSLGPF LSTENATVKL GADLLGRNST
760 770 780 790 800
IAVNSHVLSV SINKESSRVY LTDPVLFSMP HIDSDNYFNA NCSFWNYSER
810 820 830 840 850
TMMGYWSTQG CKLVDTNKTR TTCACSHLTN FAILMAHREI VYKDGVHKLL
860 870 880 890 900
LTVITWVGIV VSLVCLAICI FTFCFFRGLQ SDRNTIHKNL CINLFIAEFI
910 920 930 940 950
FLIGIDKTQY TIACPVFAGL LHFFFLAAFS WMCLEGVQLY LMLVEVFESE
960 970 980 990 1000
YSRKKYYYVA GYLFPATVVG VSAAIDSKSY GTLEACWLHV DNYFIWSFIG
1010 1020 1030 1040 1050
PVTFIILLNI IFLVITLCKM VKHSNTLKPD SSRLENINNY RVCDGYYNTD
1060 1070 1080 1090 1100
LPGYEDNKPF IKSWVLGAFA LLCLLGLTWS FGLLFVNEET VVMAYLFTAF
1110 1120 1130 1140 1150
NAFQGLFIFI FHCALQKKVR KEYAKCFRHW YCCGGLPTES PHSSVKASTS
1160 1170 1180 1190 1200
RTSARYSSGT QSRIRRMWND TVRKQSESSF ISGDINSTST LNQGMTGNYL
1210 1220 1230 1240 1250
LTNPLLRPHG TNNPYNTLLA ETVVCNAPSA PVFNSPGHSL NNTRDTSAMD
1260 1270 1280 1290 1300
TLPLNGNFNN SYSLRKADYH DGVQVVDCGL SLNDTAFEKM IISELVHNNL
1310 1320 1330 1340 1350
RGSNKTHNLE LKLPVKPVIG GSSSEDDAIV ADASSLMHGD NPGLEFRHKE
1360 1370 1380 1390 1400
LEAPLIPQRT HSLLYQPQKK VKPEATDSYV SQLTAEADEH LQSPNRDSLY
1410 1420 1430 1440 1450
TSMPNLRDSP YPESSPDMAE DLSPSRRSEN EDIYYKSMPN LGAGRQLQMC
1460 1470 1480
YQISRGNSDG YIIPINKEGC IPEGDVREGQ MQLVTSL
Length:1,487
Mass (Da):166,729
Last modified:April 26, 2004 - v2
Checksum:i56179F1A95471B00
GO
Isoform 2 (identifier: O88923-1) [UniParc]FASTAAdd to basket
Also known as: CL2BC

The sequence of this isoform differs from the canonical sequence as follows:
     1054-1062: Missing.

Show »
Length:1,478
Mass (Da):165,593
Checksum:iCBB1ED1B00F70407
GO
Isoform 3 (identifier: O88923-2) [UniParc]FASTAAdd to basket
Also known as: CL2AA

The sequence of this isoform differs from the canonical sequence as follows:
     1038-1038: N → K
     1039-1053: Missing.
     1054-1062: Missing.
     1183-1193: Missing.

Show »
Length:1,452
Mass (Da):162,744
Checksum:i3D29320F1A736660
GO
Isoform 4 (identifier: O88923-3) [UniParc]FASTAAdd to basket
Also known as: CL2AB

The sequence of this isoform differs from the canonical sequence as follows:
     1038-1038: N → K
     1039-1053: Missing.
     1054-1062: Missing.
     1194-1236: Missing.

Show »
Length:1,420
Mass (Da):159,323
Checksum:i30E8DB4FD4717BF2
GO
Isoform 5 (identifier: O88923-4) [UniParc]FASTAAdd to basket
Also known as: CL2AC

The sequence of this isoform differs from the canonical sequence as follows:
     1038-1038: N → K
     1039-1053: Missing.
     1054-1062: Missing.

Show »
Length:1,463
Mass (Da):163,875
Checksum:iD5DFAC02CC1FC3A1
GO
Isoform 6 (identifier: O88923-5) [UniParc]FASTAAdd to basket
Also known as: CL2BA

The sequence of this isoform differs from the canonical sequence as follows:
     1054-1062: Missing.
     1183-1193: Missing.

Show »
Length:1,467
Mass (Da):164,462
Checksum:i700420D1FD770513
GO
Isoform 7 (identifier: O88923-6) [UniParc]FASTAAdd to basket
Also known as: CL2BB

The sequence of this isoform differs from the canonical sequence as follows:
     1054-1062: Missing.
     1194-1236: Missing.

Show »
Length:1,435
Mass (Da):161,041
Checksum:i593833C7D30AD2D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti977S → Y in AAC62654 (PubMed:9830014).Curated1
Sequence conflicti977S → Y in AAC62655 (PubMed:9830014).Curated1
Sequence conflicti977S → Y in AAC62656 (PubMed:9830014).Curated1
Sequence conflicti977S → Y in AAC62657 (PubMed:9830014).Curated1
Sequence conflicti977S → Y in AAC62658 (PubMed:9830014).Curated1
Sequence conflicti977S → Y in AAC62659 (PubMed:9830014).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0504291038N → K in isoform 3, isoform 4 and isoform 5. 1 Publication1
Alternative sequenceiVSP_0504301039 – 1053Missing in isoform 3, isoform 4 and isoform 5. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0101121054 – 1062Missing in isoform 2, isoform 3, isoform 4, isoform 5, isoform 6 and isoform 7. 1 Publication9
Alternative sequenceiVSP_0504281183 – 1193Missing in isoform 6 and isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_0504271194 – 1236Missing in isoform 7 and isoform 4. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063102 mRNA. Translation: AAC77815.1.
AF081148 mRNA. Translation: AAC62654.1.
AF081149 mRNA. Translation: AAC62655.1.
AF081150 mRNA. Translation: AAC62656.1.
AF081151 mRNA. Translation: AAC62657.1.
AF081152 mRNA. Translation: AAC62658.1.
AF081153 mRNA. Translation: AAC62659.1.
PIRiT14324.
T17157.
T17158.
T17159.
T17160.
T17185.
T46611.
RefSeqiNP_001177404.2. NM_001190475.2.
NP_001289137.1. NM_001302208.1.
NP_001289138.1. NM_001302209.1.
NP_001289139.1. NM_001302210.1.
NP_001289140.1. NM_001302211.1.
NP_001289141.1. NM_001302212.1.
NP_599235.3. NM_134408.3.
UniGeneiRn.12089.

Genome annotation databases

GeneIDi171447.
KEGGirno:171447.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063102 mRNA. Translation: AAC77815.1.
AF081148 mRNA. Translation: AAC62654.1.
AF081149 mRNA. Translation: AAC62655.1.
AF081150 mRNA. Translation: AAC62656.1.
AF081151 mRNA. Translation: AAC62657.1.
AF081152 mRNA. Translation: AAC62658.1.
AF081153 mRNA. Translation: AAC62659.1.
PIRiT14324.
T17157.
T17158.
T17159.
T17160.
T17185.
T46611.
RefSeqiNP_001177404.2. NM_001190475.2.
NP_001289137.1. NM_001302208.1.
NP_001289138.1. NM_001302209.1.
NP_001289139.1. NM_001302210.1.
NP_001289140.1. NM_001302211.1.
NP_001289141.1. NM_001302212.1.
NP_599235.3. NM_134408.3.
UniGeneiRn.12089.

3D structure databases

ProteinModelPortaliO88923.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042136.

Chemistry databases

GuidetoPHARMACOLOGYi207.

Protein family/group databases

MEROPSiP02.009.
GPCRDBiSearch...

PTM databases

iPTMnetiO88923.
PhosphoSitePlusiO88923.

Proteomic databases

PaxDbiO88923.
PRIDEiO88923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171447.
KEGGirno:171447.

Organism-specific databases

CTDi23266.
RGDi620835. Adgrl2.

Phylogenomic databases

eggNOGiKOG3545. Eukaryota.
KOG4193. Eukaryota.
KOG4729. Eukaryota.
ENOG410XSD2. LUCA.
HOVERGENiHBG052337.
InParanoidiO88923.
KOiK04593.
PhylomeDBiO88923.

Miscellaneous databases

PROiO88923.

Family and domain databases

InterProiIPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR031240. Latrophilin-2.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like_dom.
[Graphical view]
PANTHERiPTHR12011:SF61. PTHR12011:SF61. 3 hits.
PfamiPF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTiSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRL2_RAT
AccessioniPrimary (citable) accession number: O88923
Secondary accession number(s): O88918
, O88919, O88920, O88921, O88922, Q9Z174
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.