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Protein

Adhesion G protein-coupled receptor L1

Gene

Adgrl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor probably implicated in the regulation of exocytosis.
Isoform 2: Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42CarbohydrateHMP:1

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  • cell surface receptor signaling pathway Source: InterPro
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • positive regulation of synapse assembly Source: RGD
  • positive regulation of synapse maturation Source: UniProtKB
  • synaptic vesicle exocytosis Source: RGD

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
LigandLectin

Protein family/group databases

MEROPSiP02.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor L1TAS:
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 1TAS:
Short name:
CIRL-1IBD:
Latrophilin-1TAS:
Gene namesi
Name:Adgrl1TAS:
Synonyms:CirlIBD:, Cirl1TAS:, Cl1IBD:, Lphn1TAS:
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620768. Adgrl1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 857ExtracellularIEP:Add BLAST833
Transmembranei858 – 878Helical; Name=1IEP:Add BLAST21
Topological domaini879 – 892CytoplasmicIEP:Add BLAST14
Transmembranei893 – 913Helical; Name=2IEP:Add BLAST21
Topological domaini914 – 919ExtracellularIEP:6
Transmembranei920 – 940Helical; Name=3IEP:Add BLAST21
Topological domaini941 – 964CytoplasmicIEP:Add BLAST24
Transmembranei965 – 985Helical; Name=4IEP:Add BLAST21
Topological domaini986 – 1001ExtracellularIEP:Add BLAST16
Transmembranei1002 – 1022Helical; Name=5IEP:Add BLAST21
Topological domaini1023 – 1049CytoplasmicIEP:Add BLAST27
Transmembranei1050 – 1070Helical; Name=6IEP:Add BLAST21
Topological domaini1071 – 1074ExtracellularIEP:4
Transmembranei1075 – 1095Helical; Name=7IEP:Add BLAST21
Topological domaini1096 – 1515CytoplasmicIEP:Add BLAST420

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi802S → R: Strongly reduced cleavage. 1 Publication1
Mutagenesisi804W → I: Abolishes cleavage. 1 Publication1
Mutagenesisi815W → S: Abolishes cleavage. Abolishes cell surface localization. 1 Publication1
Mutagenesisi834C → S: Strongly reduced cleavage. 2 Publications1
Mutagenesisi834C → W: Abolishes cleavage. Abolishes cell surface localization. 2 Publications1
Mutagenesisi836H → S: Abolishes cleavage. No effect on cell surface localization. 1 Publication1
Mutagenesisi837L → A: Abolishes cleavage. No effect on cell surface localization. 1 Publication1
Mutagenesisi838T → G: Abolishes cleavage. No effect on cell surface localization. 2 Publications1
Mutagenesisi838T → P: Abolishes cleavage. Abolishes cell surface localization. 2 Publications1
Mutagenesisi840F → A: Strongly reduced cleavage. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi206.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000001290825 – 1515Adhesion G protein-coupled receptor L1Add BLAST1491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 71IEP:
Disulfide bondi50 ↔ 128IEP:
Disulfide bondi83 ↔ 115IEP:
Disulfide bondi96 ↔ 102IEP:
Glycosylationi98N-linked (GlcNAc...) asparagineIEP:1
Disulfide bondi140 ↔ 322IEP:
Disulfide bondi480 ↔ 515IEP:1 Publication
Disulfide bondi503 ↔ 532IEP:1 Publication
Glycosylationi531N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi640N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi741N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi800N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi801 ↔ 832IEP:1 Publication
Glycosylationi805N-linked (GlcNAc...) asparagineIEP:1
Disulfide bondi820 ↔ 834IEP:1 Publication
Glycosylationi826N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei1237Omega-N-methylarginineHMP:1
Modified residuei1263PhosphoserineHMP:1
Modified residuei1497PhosphoserineHMP:1
Modified residuei1514PhosphoserineHMP:1

Post-translational modificationi

Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei837 – 838Cleavage; by autolysis3 Publications2

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiO88917.
PRIDEiO88917.

PTM databases

iPTMnetiO88917.
PhosphoSitePlusiO88917.
SwissPalmiO88917.

Expressioni

Tissue specificityi

Expressed in the brain (at protein level). Brain specific distribution but low levels are also detected in most tissues.2 Publications

Gene expression databases

BgeeiENSRNOG00000029134.
ExpressionAtlasiO88917. baseline and differential.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). Interacts with syntaxin and with proteins of the SHANK family via the PDZ domain (PubMed:9208860, PubMed:10958799). Isoform 2 interacts with TENM2 (PubMed:21724987). Interacts (via extracellular domain) with FLRT1, FLRT2 and FLRT3 (via extracellular domain) (By similarity).HMP:3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249249. 2 interactors.
IntActiO88917. 1 interactor.
MINTiMINT-143437.
STRINGi10116.ENSRNOP00000042610.

Structurei

Secondary structure

11515
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi483 – 485ND:3
Beta strandi488 – 490ND:3
Beta strandi498 – 502ND:5
Beta strandi508 – 515ND:8
Turni517 – 519ND:3
Helixi536 – 545ND:10
Helixi550 – 560ND:11
Helixi567 – 590ND:24
Helixi605 – 627ND:23
Helixi630 – 632ND:3
Helixi633 – 636ND:4
Helixi641 – 663ND:23
Beta strandi668 – 675ND:8
Beta strandi677 – 687ND:11
Beta strandi695 – 697ND:3
Beta strandi704 – 709ND:6
Helixi711 – 716ND:6
Beta strandi722 – 732ND:11
Helixi733 – 736ND:4
Beta strandi759 – 762ND:4
Beta strandi766 – 773ND:8
Beta strandi779 – 789ND:11
Beta strandi796 – 806ND:11
Turni808 – 810ND:3
Beta strandi813 – 816ND:4
Beta strandi820 – 825ND:6
Beta strandi827 – 836ND:10
Beta strandi839 – 847ND:9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DLQX-ray1.85A460-837[»]
B838-850[»]
ProteinModelPortaliO88917.
SMRiO88917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 129SUEL-type lectinIEP:Add BLAST90
Domaini139 – 398Olfactomedin-likeIEP:Add BLAST260
Domaini798 – 849GPSIEP:Add BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 120Carbohydrate bindingHMP:4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi413 – 416Poly-Thr4
Compositional biasi1451 – 1461Poly-ProAdd BLAST11

Domaini

The extracellular domain coupled to the a single transmembrane region are sufficient for full responsiveness to alpha-latrotoxin.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3545. Eukaryota.
KOG4193. Eukaryota.
KOG4729. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00900000140800.
HOGENOMiHOG000049065.
HOVERGENiHBG052337.
InParanoidiO88917.
KOiK04592.
PhylomeDBiO88917.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR031234. Latrophilin-1.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like_dom.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
PANTHERiPTHR12011:SF62. PTHR12011:SF62. 1 hit.
PfamiView protein in Pfam
PF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
PRINTSiPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTiView protein in SMART
SM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
PROSITEiView protein in PROSITE
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88917-1) [UniParc]FASTAAdd to basket
Also known as: CL1BB

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARLAAALWS LCVTTVLVTS ATQGLSRAGL PFGLMRRELA CEGYPIELRC
60 70 80 90 100
PGSDVIMVEN ANYGRTDDKI CDADPFQMEN VQCYLPDAFK IMSQRCNNRT
110 120 130 140 150
QCVVVAGSDA FPDPCPGTYK YLEVQYDCVP YKVEQKVFVC PGTLQKVLEP
160 170 180 190 200
TSTHESEHQS GAWCKDPLQA GDRIYVMPWI PYRTDTLTEY ASWEDYVAAR
210 220 230 240 250
HTTTYRLPNR VDGTGFVVYD GAVFYNKERT RNIVKYDLRT RIKSGETVIN
260 270 280 290 300
TANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEGNNGRL VVSQLNPYTL
310 320 330 340 350
RFEGTWETGY DKRSASNAFM VCGVLYVLRS VYVDDDSEAA GNRVDYAFNT
360 370 380 390 400
NANREEPVSL AFPNPYQFVS SVDYNPRDNQ LYVWNNYFVV RYSLEFGPPD
410 420 430 440 450
PSAGPATSPP LSTTTTARPT PLTSTASPAA TTPLRRAPLT THPVGAINQL
460 470 480 490 500
GPDLPPATAP APSTRRPPAP NLHVSPELFC EPREVRRVQW PATQQGMLVE
510 520 530 540 550
RPCPKGTRGI ASFQCLPALG LWNPRGPDLS NCTSPWVNQV AQKIKSGENA
560 570 580 590 600
ANIASELARH TRGSIYAGDV SSSVKLMEQL LDILDAQLQA LRPIERESAG
610 620 630 640 650
KNYNKMHKRE RTCKDYIKAV VETVDNLLRP EALESWKDMN ATEQVHTATM
660 670 680 690 700
LLDVLEEGAF LLADNVREPA RFLAAKQNVV LEVTVLSTEG QVQELVFPQE
710 720 730 740 750
YASESSIQLS ANTIKQNSRN GVVKVVFILY NNLGLFLSTE NATVKLAGEA
760 770 780 790 800
GTGGPGGASL VVNSQVIAAS INKESSRVFL MDPVIFTVAH LEAKNHFNAN
810 820 830 840 850
CSFWNYSERS MLGYWSTQGC RLVESNKTHT TCACSHLTNF AVLMAHREIY
860 870 880 890 900
QGRINELLLS VITWVGIVIS LVCLAICIST FCFLRGLQTD RNTIHKNLCI
910 920 930 940 950
NLFLAELLFL VGIDKTQYEV ACPIFAGLLH YFFLAAFSWL CLEGVHLYLL
960 970 980 990 1000
LVEVFESEYS RTKYYYLGGY CFPALVVGIA AAIDYRSYGT EKACWLRVDN
1010 1020 1030 1040 1050
YFIWSFIGPV SFVIVVNLVF LMVTLHKMIR SSSVLKPDSS RLDNIKSWAL
1060 1070 1080 1090 1100
GAIALLFLLG LTWAFGLLFI NKESVVMAYL FTTFNAFQGV FIFVFHCALQ
1110 1120 1130 1140 1150
KKVHKEYSKC LRHSYCCIRS PPGGAHGSLK TSAMRSNTRY YTGTQVPGQG
1160 1170 1180 1190 1200
RHIHQVSLGP RGRSALPESQ KDPGGQSGPG DPLTFGLCPS RIRRMWNDTV
1210 1220 1230 1240 1250
RKQTESSFMA GDINSTPTLN RGTMGNHLLT NPVLQPRGGT SPYNTLIAES
1260 1270 1280 1290 1300
VGFNPSSPPV FNSPGSYREP KHPLGGREAC GMDTLPLNGN FNNSYSLRSG
1310 1320 1330 1340 1350
DFPPGDGGPE PPRGRNLADA AAFEKMIISE LVHNNLRGAS GGAKGPPPEP
1360 1370 1380 1390 1400
PVPPVPGVSE DEAGGPGGAD RAEIELLYKA LEEPLLLPRA QSVLYQSDLD
1410 1420 1430 1440 1450
ESESCTAEDG ATSRPLSSPP GRDSLYASGA NLRDSPSYPD SSPEGPNEAL
1460 1470 1480 1490 1500
PPPPPAPPGP PEIYYTSRPP ALVARNPLQG YYQVRRPSHE GYLAAPSLEG
1510
PGPDGDGQMQ LVTSL
Length:1,515
Mass (Da):166,615
Last modified:November 1, 1998 - v1
Checksum:i0BF3B900C54F17B7
GO
Isoform 2 (identifier: O88917-2) [UniParc]FASTAAdd to basket
Also known as: CL1AA

The sequence of this isoform differs from the canonical sequence as follows:
     132-137: KVEQKV → I
     1146-1189: Missing.

Show »
Length:1,466
Mass (Da):161,545
Checksum:i5926609E031565F9
GO
Isoform 3 (identifier: O88917-3) [UniParc]FASTAAdd to basket
Also known as: CL1AB

The sequence of this isoform differs from the canonical sequence as follows:
     132-137: KVEQKV → I

Show »
Length:1,510
Mass (Da):166,016
Checksum:i6A22505113DFCD5B
GO
Isoform 4 (identifier: O88917-4) [UniParc]FASTAAdd to basket
Also known as: CL1BA

The sequence of this isoform differs from the canonical sequence as follows:
     1146-1189: Missing.

Show »
Length:1,471
Mass (Da):162,144
Checksum:i9F560F544B4191C6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050398132 – 137KVEQKV → I in isoform 2 and isoform 3. IBD:6
Alternative sequenceiVSP_0503991146 – 1189Missing in isoform 2 and isoform 4. IBD:Add BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081144 mRNA. Translation: AAC62650.1.
AF081145 mRNA. Translation: AAC62651.1.
AF081146 mRNA. Translation: AAC62652.1.
AF081147 mRNA. Translation: AAC62653.1.
U72487 mRNA. Translation: AAC53268.1.
U78105 mRNA. Translation: AAC98700.1.
PIRiT17138.
T17145.
T17149.
T17156.
RefSeqiNP_075251.1. NM_022962.1. [O88917-4]
UniGeneiRn.10776.

Genome annotation databases

EnsembliENSRNOT00000045699; ENSRNOP00000043269; ENSRNOG00000029134. [O88917-4]
GeneIDi65096.
KEGGirno:65096.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGRL1_RAT
AccessioniPrimary (citable) accession number: O88917
Secondary accession number(s): O09026, O35818, O88916
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 1, 1998
Last modified: November 22, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families