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Protein

Solute carrier family 22 member 8

Gene

Slc22a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Mediates the uptake of p-amino-hippurate (PAH) and estron sulfate (ES). Also mediates uptake of several organic compounds such as prostaglandin E2, prostaglandin F(2-alpha), allopurinol, 6-mercaptopurine (6-MP), 5-fluorouracil (5-FU), and L-carnitine.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Detoxification, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-561048. Organic anion transport.

Protein family/group databases

TCDBi2.A.1.19.9. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 8
Alternative name(s):
Organic anion transporter 3
Short name:
mOat3
Reduced in osteosclerosis transporter
Gene namesi
Name:Slc22a8
Synonyms:Oat3, Roct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1336187. Slc22a8.

Subcellular locationi

  • Basolateral cell membrane Curated; Multi-pass membrane protein Curated

  • Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 123ExtracellularSequence analysisAdd BLAST91
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 150CytoplasmicSequence analysis6
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 176ExtracellularSequence analysis5
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 212CytoplasmicSequence analysisAdd BLAST15
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 236ExtracellularSequence analysis3
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 327CytoplasmicSequence analysisAdd BLAST70
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 354ExtracellularSequence analysis6
Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
Topological domaini376 – 383CytoplasmicSequence analysis8
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 411ExtracellularSequence analysis7
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Topological domaini433 – 471CytoplasmicSequence analysisAdd BLAST39
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
Topological domaini493 – 537ExtracellularSequence analysisAdd BLAST45

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice appear healthly and are fertile. Exhibit a loss of taurocholate, estrone sulfate and para-aminohippurate transport in kidney and of fluorescein (FL) transport in choroid plexus.1 Publication

Chemistry databases

ChEMBLiCHEMBL2073672.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002734411 – 537Solute carrier family 22 member 8Add BLAST537

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO88909.
PaxDbiO88909.
PRIDEiO88909.

PTM databases

iPTMnetiO88909.
PhosphoSitePlusiO88909.

Expressioni

Tissue specificityi

Highly expressed in kidney. Expressed in developing bone. Weakly expressed in brain and eye.2 Publications

Gene expression databases

BgeeiENSMUSG00000063796.
GenevisibleiO88909. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010251.

Structurei

3D structure databases

ProteinModelPortaliO88909.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiO88909.
KOiK08205.
OMAiCILTHIF.
OrthoDBiEOG091G0738.
PhylomeDBiO88909.
TreeFamiTF315847.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFSEILDRV GSMGPFQYLH VTLLALPILG IANHNLLQIF TATTPDHHCR
60 70 80 90 100
PPPNASLEPW VLPLGPNGKP EKCLRFVHLP NASLPNDTQG ATEPCLDGWI
110 120 130 140 150
YNSTRDTIVT EWDLVCGSNK LKEMAQSVFM AGILVGGPVF GELSDRFGRK
160 170 180 190 200
PILTWSYLLL AASGSSAAFS PSLTVYMIFR FLCGCSISGI SLSTIILNVE
210 220 230 240 250
WVPTSTRAIS STTIGYCYTI GQFILPGLAY AVPQWRWLQL SVSAAFFIFS
260 270 280 290 300
LLSWWVPESI RWLVLSGKFS KALKTLQRVA TFNGKKEEGE KLTVEELKFN
310 320 330 340 350
LQKDITSAKV KYGLSDLFRV SILRRVTFCL SLAWFATGFA YYSLAMGVEE
360 370 380 390 400
FGVNIYILQI IFGGVDIPAK FITILSISYL GRRITQGFLL ILAGVAILAL
410 420 430 440 450
IFVSSEMQLL RTALAVFGKG CLSGSFSCLF LYTSELYPTV LRQTGMGISN
460 470 480 490 500
IWARVGSMIA PLVKITGELQ PFIPNVIFGT MTLLGGSAAF FLLETLNRPL
510 520 530
PETIEDIQDW YQQTKKTKQE PEAEKASQTI PLKTGGP
Length:537
Mass (Da):59,246
Last modified:January 23, 2007 - v2
Checksum:iF85FF82002AB26EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271K → R in AAC61265 (PubMed:10087192).Curated1
Sequence conflicti479G → V in BAC27624 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078869 mRNA. Translation: AAC61265.1.
AK031962 mRNA. Translation: BAC27624.1.
AK044336 mRNA. Translation: BAC31873.1.
AK157891 mRNA. Translation: BAE34249.1.
BC014762 mRNA. Translation: AAH14762.1.
AB079895 mRNA. Translation: BAC53618.1.
CCDSiCCDS29537.1.
RefSeqiNP_001158106.1. NM_001164634.1.
NP_001158107.1. NM_001164635.1.
NP_112471.3. NM_031194.5.
XP_011245473.1. XM_011247171.1.
UniGeneiMm.285294.

Genome annotation databases

EnsembliENSMUST00000010251; ENSMUSP00000010251; ENSMUSG00000063796.
ENSMUST00000170817; ENSMUSP00000131045; ENSMUSG00000063796.
GeneIDi19879.
KEGGimmu:19879.
UCSCiuc008gmc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078869 mRNA. Translation: AAC61265.1.
AK031962 mRNA. Translation: BAC27624.1.
AK044336 mRNA. Translation: BAC31873.1.
AK157891 mRNA. Translation: BAE34249.1.
BC014762 mRNA. Translation: AAH14762.1.
AB079895 mRNA. Translation: BAC53618.1.
CCDSiCCDS29537.1.
RefSeqiNP_001158106.1. NM_001164634.1.
NP_001158107.1. NM_001164635.1.
NP_112471.3. NM_031194.5.
XP_011245473.1. XM_011247171.1.
UniGeneiMm.285294.

3D structure databases

ProteinModelPortaliO88909.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010251.

Chemistry databases

ChEMBLiCHEMBL2073672.

Protein family/group databases

TCDBi2.A.1.19.9. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiO88909.
PhosphoSitePlusiO88909.

Proteomic databases

MaxQBiO88909.
PaxDbiO88909.
PRIDEiO88909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010251; ENSMUSP00000010251; ENSMUSG00000063796.
ENSMUST00000170817; ENSMUSP00000131045; ENSMUSG00000063796.
GeneIDi19879.
KEGGimmu:19879.
UCSCiuc008gmc.2. mouse.

Organism-specific databases

CTDi9376.
MGIiMGI:1336187. Slc22a8.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiO88909.
KOiK08205.
OMAiCILTHIF.
OrthoDBiEOG091G0738.
PhylomeDBiO88909.
TreeFamiTF315847.

Enzyme and pathway databases

ReactomeiR-MMU-561048. Organic anion transport.

Miscellaneous databases

PROiO88909.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063796.
GenevisibleiO88909. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A8_MOUSE
AccessioniPrimary (citable) accession number: O88909
Secondary accession number(s): Q3TZF9
, Q8CCX3, Q8CFH5, Q91WJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.