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O88909

- S22A8_MOUSE

UniProt

O88909 - S22A8_MOUSE

Protein

Solute carrier family 22 member 8

Gene

Slc22a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Mediates the uptake of p-amino-hippurate (PAH) and estron sulfate (ES). Also mediates uptake of several organic compounds such as prostaglandin E2, prostaglandin F(2-alpha), allopurinol, 6-mercaptopurine (6-MP), 5-fluorouracil (5-FU), and L-carnitine.3 Publications

    GO - Molecular functioni

    1. inorganic anion exchanger activity Source: Ensembl
    2. organic anion transmembrane transporter activity Source: Ensembl
    3. quaternary ammonium group transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. glutathione transport Source: Ensembl
    2. response to methotrexate Source: Ensembl
    3. response to toxic substance Source: UniProtKB-KW

    Keywords - Biological processi

    Detoxification, Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198604. Organic anion transport.

    Protein family/group databases

    TCDBi2.A.1.19.9. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 8
    Alternative name(s):
    Organic anion transporter 3
    Short name:
    mOat3
    Reduced in osteosclerosis transporter
    Gene namesi
    Name:Slc22a8
    Synonyms:Oat3, Roct
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1336187. Slc22a8.

    Subcellular locationi

    Basolateral cell membrane Curated; Multi-pass membrane protein Curated
    Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) By similarity.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice appear healthly and are fertile. Exhibit a loss of taurocholate, estrone sulfate and para-aminohippurate transport in kidney and of fluorescein (FL) transport in choroid plexus.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 537537Solute carrier family 22 member 8PRO_0000273441Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiO88909.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney. Expressed in developing bone. Weakly expressed in brain and eye.2 Publications

    Gene expression databases

    BgeeiO88909.
    GenevestigatoriO88909.

    Structurei

    3D structure databases

    ProteinModelPortaliO88909.
    SMRiO88909. Positions 117-449.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 12391ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini145 – 1506CytoplasmicSequence Analysis
    Topological domaini172 – 1765ExtracellularSequence Analysis
    Topological domaini198 – 21215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini234 – 2363ExtracellularSequence Analysis
    Topological domaini258 – 32770CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini349 – 3546ExtracellularSequence Analysis
    Topological domaini376 – 3838CytoplasmicSequence Analysis
    Topological domaini405 – 4117ExtracellularSequence Analysis
    Topological domaini433 – 47139CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini493 – 53745ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei124 – 14421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei177 – 19721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei213 – 23321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei328 – 34821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei355 – 37521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei472 – 49221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00750000117345.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiO88909.
    KOiK08205.
    OMAiDGWVYNS.
    OrthoDBiEOG7C8GH9.
    PhylomeDBiO88909.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88909-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTFSEILDRV GSMGPFQYLH VTLLALPILG IANHNLLQIF TATTPDHHCR    50
    PPPNASLEPW VLPLGPNGKP EKCLRFVHLP NASLPNDTQG ATEPCLDGWI 100
    YNSTRDTIVT EWDLVCGSNK LKEMAQSVFM AGILVGGPVF GELSDRFGRK 150
    PILTWSYLLL AASGSSAAFS PSLTVYMIFR FLCGCSISGI SLSTIILNVE 200
    WVPTSTRAIS STTIGYCYTI GQFILPGLAY AVPQWRWLQL SVSAAFFIFS 250
    LLSWWVPESI RWLVLSGKFS KALKTLQRVA TFNGKKEEGE KLTVEELKFN 300
    LQKDITSAKV KYGLSDLFRV SILRRVTFCL SLAWFATGFA YYSLAMGVEE 350
    FGVNIYILQI IFGGVDIPAK FITILSISYL GRRITQGFLL ILAGVAILAL 400
    IFVSSEMQLL RTALAVFGKG CLSGSFSCLF LYTSELYPTV LRQTGMGISN 450
    IWARVGSMIA PLVKITGELQ PFIPNVIFGT MTLLGGSAAF FLLETLNRPL 500
    PETIEDIQDW YQQTKKTKQE PEAEKASQTI PLKTGGP 537
    Length:537
    Mass (Da):59,246
    Last modified:January 23, 2007 - v2
    Checksum:iF85FF82002AB26EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti271 – 2711K → R in AAC61265. (PubMed:10087192)Curated
    Sequence conflicti479 – 4791G → V in BAC27624. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078869 mRNA. Translation: AAC61265.1.
    AK031962 mRNA. Translation: BAC27624.1.
    AK044336 mRNA. Translation: BAC31873.1.
    AK157891 mRNA. Translation: BAE34249.1.
    BC014762 mRNA. Translation: AAH14762.1.
    AB079895 mRNA. Translation: BAC53618.1.
    CCDSiCCDS29537.1.
    RefSeqiNP_001158106.1. NM_001164634.1.
    NP_001158107.1. NM_001164635.1.
    NP_112471.3. NM_031194.5.
    UniGeneiMm.285294.

    Genome annotation databases

    EnsembliENSMUST00000010251; ENSMUSP00000010251; ENSMUSG00000063796.
    ENSMUST00000170817; ENSMUSP00000131045; ENSMUSG00000063796.
    GeneIDi19879.
    KEGGimmu:19879.
    UCSCiuc008gmc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078869 mRNA. Translation: AAC61265.1 .
    AK031962 mRNA. Translation: BAC27624.1 .
    AK044336 mRNA. Translation: BAC31873.1 .
    AK157891 mRNA. Translation: BAE34249.1 .
    BC014762 mRNA. Translation: AAH14762.1 .
    AB079895 mRNA. Translation: BAC53618.1 .
    CCDSi CCDS29537.1.
    RefSeqi NP_001158106.1. NM_001164634.1.
    NP_001158107.1. NM_001164635.1.
    NP_112471.3. NM_031194.5.
    UniGenei Mm.285294.

    3D structure databases

    ProteinModelPortali O88909.
    SMRi O88909. Positions 117-449.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2073672.

    Protein family/group databases

    TCDBi 2.A.1.19.9. the major facilitator superfamily (mfs).

    Proteomic databases

    PRIDEi O88909.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010251 ; ENSMUSP00000010251 ; ENSMUSG00000063796 .
    ENSMUST00000170817 ; ENSMUSP00000131045 ; ENSMUSG00000063796 .
    GeneIDi 19879.
    KEGGi mmu:19879.
    UCSCi uc008gmc.2. mouse.

    Organism-specific databases

    CTDi 9376.
    MGIi MGI:1336187. Slc22a8.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00750000117345.
    HOGENOMi HOG000234569.
    HOVERGENi HBG108433.
    InParanoidi O88909.
    KOi K08205.
    OMAi DGWVYNS.
    OrthoDBi EOG7C8GH9.
    PhylomeDBi O88909.
    TreeFami TF315847.

    Enzyme and pathway databases

    Reactomei REACT_198604. Organic anion transport.

    Miscellaneous databases

    NextBioi 297374.
    PROi O88909.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88909.
    Genevestigatori O88909.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel putative transporter maps to the osteosclerosis (oc) mutation and is not expressed in the oc mutant mouse."
      Brady K.P., Dushkin H., Foernzler D., Koike T., Magner F., Her H., Gullans S., Segre G.V., Green R.M., Beier D.R.
      Genomics 56:254-261(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Inner ear, Medulla oblongata and Retina.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    4. "Cloning and functional characterization of mOAT3 (Roct)."
      Kobayashi Y., Shibusawa A., Ohshiro N., Sasaki T., Tokuyama S., Yamamoto T.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-537.
      Tissue: Kidney.
    5. "Impaired organic anion transport in kidney and choroid plexus of organic anion transporter 3 (Oat3 (Slc22a8)) knockout mice."
      Sweet D.H., Miller D.S., Pritchard J.B., Fujiwara Y., Beier D.R., Nigam S.K.
      J. Biol. Chem. 277:26934-26943(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Organic anion transport in choroid plexus from wild-type and organic anion transporter 3 (Slc22a8)-null mice."
      Sykes D., Sweet D.H., Lowes S., Nigam S.K., Pritchard J.B., Miller D.S.
      Am. J. Physiol. 286:F972-F978(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Renal transport of organic compounds mediated by mouse organic anion transporter 3 (mOat3): further substrate specificity of mOat3."
      Kobayashi Y., Ohshiro N., Tsuchiya A., Kohyama N., Ohbayashi M., Yamamoto T.
      Drug Metab. Dispos. 32:479-483(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiS22A8_MOUSE
    AccessioniPrimary (citable) accession number: O88909
    Secondary accession number(s): Q3TZF9
    , Q8CCX3, Q8CFH5, Q91WJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3