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Protein

Tyrosine-protein phosphatase non-receptor type 23

Gene

Ptpn23

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis (By similarity).By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1255Phosphocysteine intermediateCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 23 (EC:3.1.3.48)
Alternative name(s):
His domain-containing protein tyrosine phosphatase
Short name:
HD-PTP
Protein tyrosine phosphatase TD14
Short name:
PTP-TD14
Gene namesi
Name:Ptpn23
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619892. Ptpn23.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1255C → S: Abolishes suppressive effect on Ha-ras induced transformation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000094779‹1 – 1499Tyrosine-protein phosphatase non-receptor type 23Add BLAST›1499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei785Omega-N-methylarginineBy similarity1
Modified residuei985PhosphoserineBy similarity1
Modified residuei986PhosphoserineBy similarity1
Modified residuei994PhosphothreonineBy similarity1
Modified residuei1478Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiO88902.
PRIDEiO88902.

PTM databases

iPTMnetiO88902.
PhosphoSitePlusiO88902.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in brain, testis and kidney, and lowest levels in skeletal muscle.2 Publications

Interactioni

Subunit structurei

Interacts with GRAP2 and GRB2. Interacts with UBAP1 and CHMP4B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052992.

Structurei

3D structure databases

ProteinModelPortaliO88902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 219BRO1PROSITE-ProRule annotationAdd BLAST219
Repeati75 – 108TPR 1Add BLAST34
Repeati199 – 232TPR 2Add BLAST34
Repeati788 – 78912
Repeati790 – 79122
Repeati792 – 79332
Repeati794 – 79542
Repeati796 – 79752
Repeati798 – 79962
Repeati800 – 80172
Repeati802 – 80382
Repeati804 – 80592
Repeati806 – 807102
Repeati808 – 809112
Repeati810 – 811122
Repeati812 – 813132
Repeati814 – 815142
Repeati816 – 817152
Repeati818 – 819162
Repeati820 – 821172
Repeati822 – 823182
Repeati824 – 825192
Repeati826 – 827202
Domaini1055 – 1315Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST261

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni598 – 993HisAdd BLAST396
Regioni788 – 82720 X 2 AA approximate tandem repeats of P-QAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili278 – 305Sequence analysisAdd BLAST28
Coiled coili377 – 464Sequence analysisAdd BLAST88
Coiled coili506 – 537Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi544 – 971Pro-richAdd BLAST428
Compositional biasi1379 – 1436Pro-richAdd BLAST58

Sequence similaritiesi

Contains 1 BRO1 domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated
Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
KOG2220. Eukaryota.
ENOG410XQX6. LUCA.
HOGENOMiHOG000012993.
HOVERGENiHBG082231.
InParanoidiO88902.
PhylomeDBiO88902.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR028770. PTPN23.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PANTHERiPTHR19134:SF333. PTHR19134:SF333. 2 hits.
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM01041. BRO1. 1 hit.
SM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

O88902-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LNVNLMLGQA QECLLEKSML DNRKSFLVAR ISAQVVDYYK EACRALENPD
60 70 80 90 100
TASLLGRIQK DWKKLVQMKI YYFAAVAHLH MGKQAEEQQK FGERVAYFQS
110 120 130 140 150
ALDKLNEAIK LAKGQPDTVQ DALRFAMDVI GGKYNSAKKD NDFIYHEAVP
160 170 180 190 200
ALDTLQPVKG APLVKPLPVN PTDPAVTGPD IFAKLVPMAA HEASSLYSEE
210 220 230 240 250
KAKLLREMLA KIEDKNEVLD QFMDSMQLDP DTVDNLDAYN HIPPQLMEKC
260 270 280 290 300
AALSVRPDTV KNLVQSMQVL SGVFTDVEAS LKDIRDLLEE DELQEQKLQE
310 320 330 340 350
TLGQAGAGPG PSVTKAELGE VRREWAKYTE VHEKASFTNS ELHRAMNLHV
360 370 380 390 400
GNLRLLSGPL DQVRAALPTP ALTPEDKAVL QNLKRILAKV QEMRDQRVSL
410 420 430 440 450
EQQLRELIQK DDITASLVTT DHSEMKKLFE EQLKKYDQLK VYLEQNLAAQ
460 470 480 490 500
DNVLRALTEA NVQYAAVRRV LSELDQKWNS TLQTLVASYE AYEDLMKKSQ
510 520 530 540 550
EGKDFYADLE SKVAALLERA QSLCRAQEAA RQQLLDRELK KKAPPPRPTA
560 570 580 590 600
PKPLLSRREE GEAAEAGDQP EELRSLPPDM MAGPRLPDPF LGTAAPLHFS
610 620 630 640 650
PGPFPGSTGP ATHYLSGPLP PGTYSGPTQL MQPRAAVPMA PGPVLYPAPV
660 670 680 690 700
YTSELGLVPR SSPQHGIVSS PYAGVGPPQP IVGLPSAPPP QFSGPELAMD
710 720 730 740 750
VRPATTTVDS VQAPISSHMA LRPGPAPAPP QPCFPVPQPV PQSVPQPQPL
760 770 780 790 800
PTPYTYSIGT KQHLTGPLPQ HHFPPGIPTS FPAPRIGPQP PPQLQPQPQP
810 820 830 840 850
QPQPQPPPQP QPQPQPQPQP QPQPQPQRPV FGPQPTQQPL PFQHPHLFPS
860 870 880 890 900
QAPGILTPPP PYPFTPQPGV LGQPPPTRHT QLYPGPPPDT LPPHSGALPF
910 920 930 940 950
PSPGPPHPHP TLAYGPAPSP RPLGPQATPV SIRGPPPANQ PAPSPHLVPS
960 970 980 990 1000
PAPSPGPGPV PSRPPTAEPP PCLRRGAAAA DLLSSSPESQ HGGTQPPGGG
1010 1020 1030 1040 1050
QPLLQPTKVD AAERPTAQAL RLIEQDPYEH PERLQKLQQE LESFRGQLGD
1060 1070 1080 1090 1100
AGALDAVWRE LQEAQEHDAR GRSIAIARCY SLKNRHQDVM PYDSNRVVLR
1110 1120 1130 1140 1150
SGKDDYINAS CVEGLSPYCP PLVATQRPLP GTAADFWLMV HEQKVSVIVM
1160 1170 1180 1190 1200
LVSEAEMEKQ KVARYFPIER GQPMVHGALS VALSSVRTTD THVERVLSLQ
1210 1220 1230 1240 1250
FRDQSLKRSL VHLHFPTWPE LGLPDSPGNL LRFIQEVHAH YLHQRPLHTP
1260 1270 1280 1290 1300
IVVHCSSGVG RTGAFALLYA AVQEVEAGSR IPELPQLVRR MRQQRKHMLQ
1310 1320 1330 1340 1350
EKLHLKFCHE ALVRHVEQVL QRHGVPPPGK PVASMSVSQK SHLPQDSQDL
1360 1370 1380 1390 1400
VLGGDVPISS IQATIAKLSI RPLGGLDSPA ASLPSLVEPP GLPPASLPEP
1410 1420 1430 1440 1450
TPAPPSSPPP PSSPLPEPPQ PEEEPSVPEA PSLGPPSSSL ELLASLTPEA
1460 1470 1480 1490
FSLDSSLRGK QRMSKQNFLQ AHNGQGLRAA QPTDDPLSLL DPLWTLNKT
Length:1,499
Mass (Da):163,454
Last modified:June 21, 2005 - v2
Checksum:i9F9BF8C8DE517123
GO

Sequence cautioni

The sequence AAC62959 differs from that shown. Reason: Frameshift at positions 1302 and 1364.Curated
The sequence AAC62959 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti1280R → G in AAF13172 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077000 mRNA. Translation: AAC62959.1. Sequence problems.
AF175208 mRNA. Translation: AAF13172.1.
PIRiT14355.
UniGeneiRn.54442.

Genome annotation databases

UCSCiRGD:619892. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077000 mRNA. Translation: AAC62959.1. Sequence problems.
AF175208 mRNA. Translation: AAF13172.1.
PIRiT14355.
UniGeneiRn.54442.

3D structure databases

ProteinModelPortaliO88902.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052992.

PTM databases

iPTMnetiO88902.
PhosphoSitePlusiO88902.

Proteomic databases

PaxDbiO88902.
PRIDEiO88902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:619892. rat.

Organism-specific databases

RGDi619892. Ptpn23.

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
KOG2220. Eukaryota.
ENOG410XQX6. LUCA.
HOGENOMiHOG000012993.
HOVERGENiHBG082231.
InParanoidiO88902.
PhylomeDBiO88902.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR028770. PTPN23.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PANTHERiPTHR19134:SF333. PTHR19134:SF333. 2 hits.
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM01041. BRO1. 1 hit.
SM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTN23_RAT
AccessioniPrimary (citable) accession number: O88902
Secondary accession number(s): Q9QZP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.