Reviewed, UniProtKB/Swiss-Prot O88900 (GRB14_RAT)

Names and origin

Protein names

Recommended name:
    Growth factor receptor-bound protein 14
Alternative name(s):
    GRB14 adapter protein

Gene names

Name:

Grb14

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain.
Subunit structure	Binds to the ankyrin repeat region of TNKL via its N-terminus By similarity.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity.
Post-translational modification	Phosphorylated on serine residues By similarity.
Sequence similarities	Belongs to the GRB7/10/14 family. Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endosome Golgi apparatus Membrane
Domain	SH2 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Ref.1 Inferred from expression pattern. Source: RGD negative regulation of insulin receptor signaling pathway Ref.1 Inferred from direct assay. Source: RGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	insulin receptor binding Ref.1 Inferred from physical interaction. Source: RGD phosphoprotein binding Ref.1 Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Growth factor receptor-bound protein 14		PRO_0000150350
Regions
	Domain	104 – 190	87	Ras-associating
	Domain	232 – 340	109	PH
	Domain	437 – 533	97	SH2
Amino acid modifications
	Modified residue	370	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88900-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CEBC9037E7868EEF	FASTA	538	60,593
	10 20 30 40 50 60 MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP ASDATRRGAM 70 80 90 100 110 120 DRRKAKDLEV QETPSIPNPF PELCCSPLTS VLSAGLFPRS NSRKKQVIKV YSEDETSRAL 130 140 150 160 170 180 EVPSDVTARD VCQLLILKNH YVDDNSWTLF EHLSHTGVER TVEDHELLTE VLSHWVMEED 190 200 210 220 230 240 NKLYLRKNYA KYEFFKNPMY FFPEHMVSFA TEMNGDRSLT QIPQVFLSSN TYPEIHGFLH 250 260 270 280 290 300 AKEQGKKSWK KAYFFLRRSG LYFSTKGTSK EPRHLQFFSE FSTSNVYMSL AGKKKHGAPT 310 320 330 340 350 360 PYGFCFKPTK AGGPRDLKML CAEEDQSRMC WVTAIRLLKY GMQLYQNYMH PSQARSACSS 370 380 390 400 410 420 QSVSPMRSVS ENSLVAMDFS GQKTRVIDNP TEALSVAVEE GLAWRKKGCL RLGNHGSPTA 430 440 450 460 470 480 PSQSSAVNMA LHRSQPWFHH RISRDEAQQL ITRQGPVDGV FLVRDSQSNP RTFVLSMSHG 490 500 510 520 530 QKIKHFQIIP VEDDGEVFHT LDDGHTKFTD LIQLVEFYQL NKGVLPCKLK HYCARMAV

References

[1]	"Identification of the rat adapter Grb14 as an inhibitor of insulin actions." Kasus-Jacobi A., Perdereau D., Auzan C., Clauser E., van Obberghen E., Mauvais-Jarvis F., Girard J., Burnol A.-F. J. Biol. Chem. 273:26026-26035(1998) [PubMed: 9748281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF076619 mRNA. Translation: AAC61478.1.
IPI	IPI00214149.
RefSeq	NP_113811.1.
UniGene	Rn.30028
3D structure databases
SMR	O88900. Positions 100-191, 228-345, 371-407, 431-535.
DisProt	DP00490.
ModBase	Search...
Protein-protein interaction databases
STRING	O88900.
PTM databases
PhosphoSite	O88900.
Genome annotation databases
Ensembl	ENSRNOT00000046721; ENSRNOP00000042214; ENSRNOG00000031396; Rattus norvegicus. [Genome view]
GeneID	58844.
KEGG	rno:58844.
Organism-specific databases
CTD	58844.
RGD	61869. Grb14.
Phylogenomic databases
eggNOG	roNOG14762.
HOVERGEN	O88900.
InParanoid	O88900.
Gene expression databases
ArrayExpress	O88900.
Genevestigator	O88900.
GermOnline	ENSRNOG00000031396. Rattus norvegicus.
Family and domain databases
InterPro	IPR015042. BPS-dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000159. Ras-assoc. IPR000980. SH2. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF08947. BPS. 1 hit. PF00169. PH. 1 hit. PF00788. RA. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN.
SMART	SM00233. PH. 1 hit. SM00314. RA. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50200. RA. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611417.

Entry information

Entry name

GRB14_RAT

Accession

Primary (citable) accession number: O88900

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	January 19, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)