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Protein

Growth factor receptor-bound protein 14

Gene

Grb14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to insulin stimulus Source: CAFA
  • insulin receptor signaling pathway Source: RGD
  • intracellular signal transduction Source: RGD
  • negative regulation of insulin receptor signaling pathway Source: RGD
  • negative regulation of meiotic cell cycle process involved in oocyte maturation Source: CAFA

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 14
Alternative name(s):
GRB14 adapter protein
Gene namesi
Name:Grb14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61869 Grb14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001503502 – 538Growth factor receptor-bound protein 14Add BLAST537

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei370PhosphoserineCombined sources1
Modified residuei373PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO88900
PRIDEiO88900

PTM databases

iPTMnetiO88900
PhosphoSitePlusiO88900

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor, through the SH2 domain. Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation (By similarity). Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FGFR1P113623EBI-7639197,EBI-1028277From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248645, 2 interactors
IntActiO88900, 4 interactors
MINTiO88900
STRINGi10116.ENSRNOP00000046233

Structurei

3D structure databases

DisProtiDP00490
ProteinModelPortaliO88900
SMRiO88900
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 190Ras-associatingPROSITE-ProRule annotationAdd BLAST87
Domaini232 – 340PHPROSITE-ProRule annotationAdd BLAST109
Domaini437 – 533SH2PROSITE-ProRule annotationAdd BLAST97

Domaini

The PH domain binds relatively non-specifically and with low affinity to several phosphoinositides, the best binder being PI(3,4,5)P3.

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3751 Eukaryota
ENOG410XXC8 LUCA
HOGENOMiHOG000231904
HOVERGENiHBG000468
InParanoidiO88900
PhylomeDBiO88900

Family and domain databases

CDDicd10414 SH2_Grb14, 1 hit
Gene3Di2.30.29.30, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR015042 BPS-dom
IPR035035 Grb14
IPR035034 Grb14_SH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000159 RA_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR11243:SF22 PTHR11243:SF22, 1 hit
PfamiView protein in Pfam
PF08947 BPS, 1 hit
PF00169 PH, 1 hit
PF00788 RA, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00314 RA, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50200 RA, 1 hit
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP
60 70 80 90 100
ASDATRRGAM DRRKAKDLEV QETPSIPNPF PELCCSPLTS VLSAGLFPRS
110 120 130 140 150
NSRKKQVIKV YSEDETSRAL EVPSDVTARD VCQLLILKNH YVDDNSWTLF
160 170 180 190 200
EHLSHTGVER TVEDHELLTE VLSHWVMEED NKLYLRKNYA KYEFFKNPMY
210 220 230 240 250
FFPEHMVSFA TEMNGDRSLT QIPQVFLSSN TYPEIHGFLH AKEQGKKSWK
260 270 280 290 300
KAYFFLRRSG LYFSTKGTSK EPRHLQFFSE FSTSNVYMSL AGKKKHGAPT
310 320 330 340 350
PYGFCFKPTK AGGPRDLKML CAEEDQSRMC WVTAIRLLKY GMQLYQNYMH
360 370 380 390 400
PSQARSACSS QSVSPMRSVS ENSLVAMDFS GQKTRVIDNP TEALSVAVEE
410 420 430 440 450
GLAWRKKGCL RLGNHGSPTA PSQSSAVNMA LHRSQPWFHH RISRDEAQQL
460 470 480 490 500
ITRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHFQIIP VEDDGEVFHT
510 520 530
LDDGHTKFTD LIQLVEFYQL NKGVLPCKLK HYCARMAV
Length:538
Mass (Da):60,593
Last modified:November 1, 1998 - v1
Checksum:iCEBC9037E7868EEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076619 mRNA Translation: AAC61478.1
RefSeqiNP_113811.1, NM_031623.1
UniGeneiRn.30028

Genome annotation databases

GeneIDi58844
KEGGirno:58844
UCSCiRGD:61869 rat

Similar proteinsi

Entry informationi

Entry nameiGRB14_RAT
AccessioniPrimary (citable) accession number: O88900
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health