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Protein

A-kinase anchor protein 1, mitochondrial

Gene

Akap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.

GO - Molecular functioni

  • beta-tubulin binding Source: RGD
  • microtubule binding Source: RGD
  • protein complex scaffold Source: RGD
  • protein domain specific binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD
  • protein kinase binding Source: RGD
  • protein phosphatase 2B binding Source: RGD
  • protein phosphatase binding Source: RGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to cAMP Source: RGD
  • cellular response to peptide hormone stimulus Source: RGD
  • negative regulation of cardiac muscle hypertrophy Source: RGD
  • negative regulation of NFAT protein import into nucleus Source: RGD
  • negative regulation of protein dephosphorylation Source: RGD
  • regulation of protein kinase A signaling Source: RGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 1, mitochondrial
Alternative name(s):
A-kinase anchor protein 121 kDa
Short name:
AKAP 121
Dual specificity A-kinase-anchoring protein 1
Short name:
D-AKAP-1
Protein kinase A-anchoring protein 1
Short name:
PRKA1
Spermatid A-kinase anchor protein 84
Short name:
S-AKAP84
Gene namesi
Name:Akap1
Synonyms:Akap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620826. Akap1.

Subcellular locationi

  • Mitochondrion outer membrane By similarity

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: RGD
  • mitochondrial crista Source: RGD
  • mitochondrial inner membrane Source: RGD
  • mitochondrial matrix Source: RGD
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • neuromuscular junction Source: RGD
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionBy similarityAdd
BLAST
Chaini30 – 854825A-kinase anchor protein 1, mitochondrialPRO_0000016661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei101 – 1011PhosphoserineCombined sources
Modified residuei103 – 1031PhosphoserineCombined sources
Modified residuei164 – 1641PhosphoserineBy similarity
Modified residuei398 – 3981PhosphothreonineBy similarity
Modified residuei484 – 4841PhosphothreonineBy similarity
Modified residuei524 – 5241PhosphoserineBy similarity
Modified residuei543 – 5431PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88884.
PRIDEiO88884.

PTM databases

PhosphoSiteiO88884.

Expressioni

Tissue specificityi

Testis specific.

Inductioni

By thyroid stimulating hormone (TSH) and cAMP or cAMP-analog.

Interactioni

Subunit structurei

Interacts with SLC8A3.By similarity

GO - Molecular functioni

  • beta-tubulin binding Source: RGD
  • microtubule binding Source: RGD
  • protein complex scaffold Source: RGD
  • protein domain specific binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD
  • protein kinase binding Source: RGD
  • protein phosphatase 2B binding Source: RGD
  • protein phosphatase binding Source: RGD

Protein-protein interaction databases

IntActiO88884. 2 interactions.
MINTiMINT-4584996.
STRINGi10116.ENSRNOP00000052543.

Structurei

3D structure databases

ProteinModelPortaliO88884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 62265KHPROSITE-ProRule annotationAdd
BLAST
Domaini709 – 76860TudorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni303 – 31614PKA-RII subunit binding domainAdd
BLAST

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Contains 1 KH domain.PROSITE-ProRule annotation
Contains 1 Tudor domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane

Phylogenomic databases

eggNOGiKOG2279. Eukaryota.
ENOG410Z0DP. LUCA.
HOGENOMiHOG000013170.
HOVERGENiHBG057436.
InParanoidiO88884.
KOiK16518.
PhylomeDBiO88884.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR033104. Akap1.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR018459. RII_binding_1.
IPR002999. Tudor.
[Graphical view]
PANTHERiPTHR22948:SF18. PTHR22948:SF18. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF10522. RII_binding_1. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88884-1) [UniParc]FASTAAdd to basket

Also known as: AKAP121

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIQFRSLFP LALPGMLALL GWWWFFSRKK DRLSSNGKQV GTLKVGPAIE
60 70 80 90 100
DRLPTEEACP GVLSVTPSVT QPPGKEEQRS MDRPLSDPPA LPRTRQVRRR
110 120 130 140 150
SESSGNLPSI VDTRLQAGQC SDENSKVVLS LMGDEAKSIP LGRPLFPKDL
160 170 180 190 200
SFPYEAVEGC KQESALGRTP GRGWLSQCAA SGENARETGG AEGTGDAVLG
210 220 230 240 250
ESVLEEGLLP QECVSEVEKS EFPILAPGGG GGEKVRSGPP QVDELLKKEE
260 270 280 290 300
YIVGKLPSSF VGPVHSELVK DEGALVPQVK GSQDRSLARE LDKDKTLPEK
310 320 330 340 350
DQIEQTAFQI ISQVILEATE EIRATTVGKT VAQVHPTPGT QPQGQEESCV
360 370 380 390 400
PASQETSLGQ EIPDPASTRT GATASPSAGA PPPKTYVSCL SSPLSGPTKD
410 420 430 440 450
QKPKNSAHHI SLAPCPPPVT PQRQSLDGAS NPRGDDTFVT CTSNNSQSVL
460 470 480 490 500
SVTSLGLCSD PVSTSRLEDS CTETISSSGD KAVTPPLPDS TEPFSNGVLK
510 520 530 540 550
EELSDLGTED GWTMDTEADH SGGSDGNSMD SVDSCCGLTK PDSPQTVQAG
560 570 580 590 600
SNPKKVDLII WEIEVPKHLV GRLIGKQGRY VSFLKQTSGA KIYISTLPYT
610 620 630 640 650
QNIQICHIEG SQHHVDKALN LIGKKFKELN LTNIYAPPLP SLALPSLPMT
660 670 680 690 700
SWLMLPDGIT VEVIVVNQVN AGHLFVQQHT HPTFHALRSL DQQMYLCYSQ
710 720 730 740 750
PGIPTLPTPV EITVICAAPG ADGAWWRAQV VASYEETNEV EIRYVDYGGY
760 770 780 790 800
KRVKVDVLRQ IRSDFVTLPF QGAEVLLDSV VPLSDDDHFS PEADAAMSEM
810 820 830 840 850
TGNTALLAQV TSYSATGLPL IQLWSVVGDE VVLINRSLVE RGLAQWVDSC

YASL
Length:854
Mass (Da):91,747
Last modified:November 1, 1998 - v1
Checksum:i6EFBA30F8801A06E
GO
Isoform 2 (identifier: O88884-2) [UniParc]FASTAAdd to basket

Also known as: S-AKAP84

The sequence of this isoform differs from the canonical sequence as follows:
     523-544: GSDGNSMDSVDSCCGLTKPDSP → VAAPPQERGHFGNGGCAGFFEC
     545-854: Missing.

Show »
Length:544
Mass (Da):57,568
Checksum:iFFB0CA47ABCC33A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti311 – 3111I → T in AAC61775 (PubMed:9722570).Curated
Sequence conflicti439 – 4391V → A in AAC61775 (PubMed:9722570).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei523 – 54422GSDGN…KPDSP → VAAPPQERGHFGNGGCAGFF EC in isoform 2. 1 PublicationVSP_002854Add
BLAST
Alternative sequencei545 – 854310Missing in isoform 2. 1 PublicationVSP_002855Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068202 mRNA. Translation: AAC33895.1.
AF092523 mRNA. Translation: AAC61775.1.
RefSeqiNP_446117.1. NM_053665.1. [O88884-1]
UniGeneiRn.163146.

Genome annotation databases

GeneIDi114124.
KEGGirno:114124.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068202 mRNA. Translation: AAC33895.1.
AF092523 mRNA. Translation: AAC61775.1.
RefSeqiNP_446117.1. NM_053665.1. [O88884-1]
UniGeneiRn.163146.

3D structure databases

ProteinModelPortaliO88884.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88884. 2 interactions.
MINTiMINT-4584996.
STRINGi10116.ENSRNOP00000052543.

PTM databases

PhosphoSiteiO88884.

Proteomic databases

PaxDbiO88884.
PRIDEiO88884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114124.
KEGGirno:114124.

Organism-specific databases

CTDi8165.
RGDi620826. Akap1.

Phylogenomic databases

eggNOGiKOG2279. Eukaryota.
ENOG410Z0DP. LUCA.
HOGENOMiHOG000013170.
HOVERGENiHBG057436.
InParanoidiO88884.
KOiK16518.
PhylomeDBiO88884.

Miscellaneous databases

NextBioi618357.
PROiO88884.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR033104. Akap1.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR018459. RII_binding_1.
IPR002999. Tudor.
[Graphical view]
PANTHERiPTHR22948:SF18. PTHR22948:SF18. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF10522. RII_binding_1. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a kinase anchor protein 121 is regulated by hormones in thyroid and testicular germ cells."
    Feliciello A., Rubin C.S., Avvedimento E.V., Gottesman M.E.
    J. Biol. Chem. 273:23361-23366(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Thyroid.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101 AND SER-103, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAKAP1_RAT
AccessioniPrimary (citable) accession number: O88884
Secondary accession number(s): O88980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1998
Last modified: April 13, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.