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Reviewed, UniProtKB/Swiss-Prot O88879 (APAF_MOUSE)

Last modified January 19, 2010. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Apoptotic protease-activating factor 1
      Short name=Apaf-1
Gene names
Name: Apaf1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1249 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase 9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP By similarity.

Subunit structure

Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex By similarity. Interacts with UACA. It may also interact with Bcl-XL. Interacts with APIP. Ref.3 Ref.4

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Highly expressed in lung and spleen, weakly in brain and kidney and not detectable in liver.

Developmental stage

High levels in embryonic brain and liver from E11.5 to E17.5 day.

Domain

The CARD domain mediates interaction with APIP. Ref.3

Sequence similarities

Contains 1 CARD domain.

Contains 1 NB-ARC domain.

Contains 13 WD repeats.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ppp1caP621371EBI-1202386,EBI-357187

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O88879-1)

Also known as: Apaf-1L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Major isoform.
Isoform 2 (identifier: O88879-2)

The sequence of this isoform differs from the canonical sequence as follows:
     99-110: GKDTDGGITSFV → A

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12491249Apoptotic protease-activating factor 1
PRO_0000050845

Regions

Domain1 – 9090CARD
Domain106 – 415310NB-ARC
Repeat613 – 65240WD 1
Repeat655 – 69440WD 2
Repeat697 – 73842WD 3
Repeat741 – 78040WD 4
Repeat796 – 83742WD 5
Repeat838 – 87740WD 6
Repeat880 – 91940WD 7
Repeat959 – 99840WD 8
Repeat1001 – 104040WD 9
Repeat1042 – 108039WD 10
Repeat1083 – 112240WD 11
Repeat1125 – 116440WD 12
Repeat1176 – 121338WD 13
Nucleotide binding154 – 1618ATP Potential

Amino acid modifications

Modified residue2381Phosphoserine By similarity

Natural variations

Alternative sequence99 – 11012GKDTD…ITSFV → A in isoform 2.
VSP_006763

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Apaf-1L) [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 53D91539CEF6025C

FASTA1,249140,889
        10         20         30         40         50         60 
MDAKARNCLL QHREALEKDI KTSYIMDHMI SNGVLSVIEE EKVKSQATQY QRAAALIKMI 

        70         80         90        100        110        120 
LNKDNCAYIS FYNALLHEGY KDLAALLQSG LPLVSSSSGK DTDGGITSFV RTVLCEGGVP 

       130        140        150        160        170        180 
QRPVIFVTRK KLVHAIQQKL WKLNGEPGWV TIYGMAGCGK SVLAAEAVRD HSLLEGCFSG 

       190        200        210        220        230        240 
GVHWVSIGKQ DKSGLLMKLQ NLCMRLDQDE SFSQRLPLNI EEAKDRLRVL MLRKHPRSLL 

       250        260        270        280        290        300 
ILDDVWDPWV LKAFDNQCQI LLTTRDKSVT DSVMGPKHVV PVESGLGREK GLEILSLFVN 

       310        320        330        340        350        360 
MKKEDLPAEA HSIIKECKGS PLVVSLIGAL LRDFPNRWAY YLRQLQNKQF KRIRKSSSYD 

       370        380        390        400        410        420 
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CVLWDLETEE VEDILQEFVN 

       430        440        450        460        470        480 
KSLLFCNRNG KSFCYYLHDL QVDFLTEKNR SQLQDLHRKM VTQFQRYYQP HTLSPDQEDC 

       490        500        510        520        530        540 
MYWYNFLAYH MASANMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVAYR HILDEKDCAV 

       550        560        570        580        590        600 
CENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYRQA KLQAKQEGDT GPLYLEWINK 

       610        620        630        640        650        660 
KTIKNLSRLV VRPHTDAVYH ACFSQDGQRI ASCGADKTLQ VFKAETGEKL LDIKAHEDEV 

       670        680        690        700        710        720 
LCCAFSSDDS YIATCSADKK VKIWDSATGK LVHTYDEHSE QVNCCHFTNS SNHLLLATGS 

       730        740        750        760        770        780 
NDFFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDELLAS CSADGTLRLW DVRSANERKS 

       790        800        810        820        830        840 
INVKRFFLSS EDPPEDVEVI VKCCSWSADG DKIIVAAKNK VLLFDIHTSG LLAEIHTGHH 

       850        860        870        880        890        900 
STIQYCDFSP YDHLAVIALS QYCVELWNID SRLKVADCRG HLSWVHGVMF SPDGSSFLTA 

       910        920        930        940        950        960 
SDDQTIRVWE TKKVCKNSAI VLKQEIDVVF QENETMVLAV DNIRGLQLIA GKTGQIDYLP 

       970        980        990       1000       1010       1020 
EAQVSCCCLS PHLEYVAFGD EDGAIKIIEL PNNRVFSSGV GHKKAVRHIQ FTADGKTLIS 

      1030       1040       1050       1060       1070       1080 
SSEDSVIQVW NWQTGDYVFL QAHQETVKDF RLLQDSRLLS WSFDGTVKVW NVITGRIERD 

      1090       1100       1110       1120       1130       1140 
FTCHQGTVLS CAISSDATKF SSTSADKTAK IWSFDLLSPL HELKGHNGCV RCSAFSLDGI 

      1150       1160       1170       1180       1190       1200 
LLATGDDNGE IRIWNVSDGQ LLHSCAPISV EEGTATHGGW VTDVCFSPDS KTLVSAGGYL 

      1210       1220       1230       1240 
KWWNVATGDS SQTFYTNGTN LKKIHVSPDF RTYVTVDNLG ILYILQVLE

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Isoform 2.

Checksum: 35216F6324DEB4A7
Show »

FASTA1,238139,782

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References

[1]"Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development."
Cecconi F., Alvarez-Bolado G., Meyer B.I., Roth K.A., Gruss P.
Cell 94:727-737(1998) [PubMed: 9753320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Swiss Webster / NIH.
Tissue: Embryo.
[2]"A comparison of the expression and properties of Apaf-1 and Apaf-1L."
Walke D.W., Morgan J.I.
Brain Res. 886:73-81(2000) [PubMed: 11119689] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Spleen.
[3]"Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein."
Cho D.-H., Hong Y.-M., Lee H.-J., Woo H.-N., Pyo J.-O., Mak T.W., Jung Y.-K.
J. Biol. Chem. 279:39942-39950(2004) [PubMed: 15262985] [Abstract]
Cited for: INTERACTION WITH APIP, DOMAIN.
[4]"Nucling recruits Apaf-1/pro-caspase-9 complex for the induction of stress-induced apoptosis."
Sakai T., Liu L., Teng X., Mukai-Sakai R., Shimada H., Kaji R., Mitani T., Matsumoto M., Toida K., Ishimura K., Shishido Y., Mak T.W., Fukui K.
J. Biol. Chem. 279:41131-41140(2004) [PubMed: 15271982] [Abstract]
Cited for: INTERACTION WITH UACA.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF064071 mRNA. Translation: AAC62458.1.
IPIIPI00135366.
IPI00654978.
UniGeneMm.220289

3D structure databases

SMRO88879. Positions 1-586, 502-775, 620-1244.
ModBaseSearch...

Protein-protein interaction databases

IntActO88879. 1 interaction.
STRINGO88879.

PTM databases

PhosphoSiteO88879.

Proteomic databases

PRIDEO88879.

Genome annotation databases

EnsemblENSMUST00000020157; ENSMUSP00000020157; ENSMUSG00000019979; Mus musculus. [Genome view]
UCSCuc007gti.1. mouse.

Organism-specific databases

MGIMGI:1306796. Apaf1.

Phylogenomic databases

HOVERGENO88879.
InParanoidO88879.
PhylomeDBO88879.

Gene expression databases

ArrayExpressO88879.
BgeeO88879.
CleanExMM_APAF1.
GenevestigatorO88879.
GermOnlineENSMUSG00000019979. Mus musculus.

Family and domain databases

InterProIPR017251. Apoptotic_pept-activating_1.
IPR001315. CARD.
IPR011029. DEATH-like.
IPR000767. Disease_R.
IPR020472. G-protein_beta_WD-40_rep_reg.
IPR002182. NB-ARC.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019782. WD40_repeat_2.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:1.10.533.10. DEATH_like. 1 hit.
G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
PfamPF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 11 hits.
[Graphical view]
PIRSFPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSPR00364. DISEASERSIST.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 13 hits.
[Graphical view]
PROSITEPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameAPAF_MOUSE
AccessionPrimary (citable) accession number: O88879
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: January 19, 2010
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents