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Protein

Short-chain dehydrogenase/reductase 3

Gene

Dhrs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.By similarity

Catalytic activityi

All-trans-retinol + NADP+ = all-trans-retinal + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei175 – 1751SubstrateBy similarity
Active sitei188 – 1881Proton acceptorBy similarity

GO - Molecular functioni

  1. NADP-retinol dehydrogenase activity Source: UniProtKB-EC
  2. retinol dehydrogenase activity Source: MGI

GO - Biological processi

  1. bone morphogenesis Source: MGI
  2. cardiac septum morphogenesis Source: MGI
  3. negative regulation of retinoic acid receptor signaling pathway Source: MGI
  4. outflow tract morphogenesis Source: MGI
  5. palate development Source: MGI
  6. regulation of ossification Source: MGI
  7. retinoid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_280434. The retinoid cycle in cones (daylight vision).
REACT_310468. RA biosynthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Short-chain dehydrogenase/reductase 3 (EC:1.1.1.300)
Alternative name(s):
Retinal short-chain dehydrogenase/reductase 1
Short name:
retSDR1
Gene namesi
Name:Dhrs3
Synonyms:Rsdr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1315215. Dhrs3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence AnalysisAdd
BLAST
Transmembranei258 – 27821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Short-chain dehydrogenase/reductase 3PRO_0000054645Add
BLAST

Proteomic databases

MaxQBiO88876.
PaxDbiO88876.
PRIDEiO88876.

PTM databases

PhosphoSiteiO88876.

Expressioni

Tissue specificityi

In the embryo, expressed in developing osteogenic and chondrogenic tissues of vertebra, rib, tooth and limb bud.1 Publication

Gene expression databases

BgeeiO88876.
CleanExiMM_DHRS3.
ExpressionAtlasiO88876. baseline and differential.
GenevestigatoriO88876.

Structurei

3D structure databases

ProteinModelPortaliO88876.
SMRiO88876. Positions 34-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00540000069900.
HOVERGENiHBG051352.
InParanoidiO88876.
KOiK11146.
OMAiWTMHALI.
OrthoDBiEOG7Z3F50.
PhylomeDBiO88876.
TreeFamiTF312837.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88876-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWKWLGALV VFPLQMIYLV TKAAVGMVLP PKLRDLSRES VLITGGGRGI
60 70 80 90 100
GRHLAREFAE RGARKIVLWG RTEKCLKETT EEIRQMGTEC HYFICDVGNR
110 120 130 140 150
EEVYQMAKAV REKVGDITIL VNNAAVVHGK SLMDSDDDAL LKSQHVNTLG
160 170 180 190 200
QFWTTKAFLP RMLELQNGHI VCLNSVLALS AIPGAIDYCT SKASAFAFME
210 220 230 240 250
SLTLGLLDCP GVSATTVLPF HTSTEMFQGM RVRFPNLFPP LKPETVARRT
260 270 280 290 300
VDAVQQNQAL LLLPWTMNIL IILKSILPQA ALEEIHRFSG TYTCMNTFKG

RT
Length:302
Mass (Da):33,652
Last modified:May 9, 2004 - v2
Checksum:iE530F36877C3C610
GO
Isoform 2 (identifier: O88876-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     114-153: Missing.

Note: No experimental confirmation available.

Show »
Length:236
Mass (Da):26,431
Checksum:i49DD2E6FC940669A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → F in AAH10972 (PubMed:15489334).Curated
Sequence conflicti234 – 2341F → L (PubMed:9705317).Curated
Sequence conflicti234 – 2341F → L (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2626Missing in isoform 2. 1 PublicationVSP_050734Add
BLAST
Alternative sequencei114 – 15340Missing in isoform 2. 1 PublicationVSP_050735Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061743 mRNA. Translation: AAC63265.1.
X95281 mRNA. Translation: CAA64602.1.
AF179241
, AF179238, AF179239, AF179240 Genomic DNA. Translation: AAD55403.1.
AK032958 mRNA. Translation: BAC28098.1.
AK151419 mRNA. Translation: BAE30384.1.
BC008980 mRNA. Translation: AAH08980.1.
BC010972 mRNA. Translation: AAH10972.1.
BC013540 mRNA. Translation: AAH13540.1.
CCDSiCCDS18912.1. [O88876-1]
RefSeqiNP_001165895.1. NM_001172424.1.
NP_035433.1. NM_011303.6. [O88876-1]
UniGeneiMm.14063.

Genome annotation databases

EnsembliENSMUST00000105744; ENSMUSP00000101370; ENSMUSG00000066026. [O88876-2]
ENSMUST00000154208; ENSMUSP00000122552; ENSMUSG00000066026. [O88876-1]
GeneIDi20148.
KEGGimmu:20148.
UCSCiuc008vrn.2. mouse. [O88876-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061743 mRNA. Translation: AAC63265.1.
X95281 mRNA. Translation: CAA64602.1.
AF179241
, AF179238, AF179239, AF179240 Genomic DNA. Translation: AAD55403.1.
AK032958 mRNA. Translation: BAC28098.1.
AK151419 mRNA. Translation: BAE30384.1.
BC008980 mRNA. Translation: AAH08980.1.
BC010972 mRNA. Translation: AAH10972.1.
BC013540 mRNA. Translation: AAH13540.1.
CCDSiCCDS18912.1. [O88876-1]
RefSeqiNP_001165895.1. NM_001172424.1.
NP_035433.1. NM_011303.6. [O88876-1]
UniGeneiMm.14063.

3D structure databases

ProteinModelPortaliO88876.
SMRiO88876. Positions 34-290.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO88876.

Proteomic databases

MaxQBiO88876.
PaxDbiO88876.
PRIDEiO88876.

Protocols and materials databases

DNASUi20148.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105744; ENSMUSP00000101370; ENSMUSG00000066026. [O88876-2]
ENSMUST00000154208; ENSMUSP00000122552; ENSMUSG00000066026. [O88876-1]
GeneIDi20148.
KEGGimmu:20148.
UCSCiuc008vrn.2. mouse. [O88876-1]

Organism-specific databases

CTDi9249.
MGIiMGI:1315215. Dhrs3.

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00540000069900.
HOVERGENiHBG051352.
InParanoidiO88876.
KOiK11146.
OMAiWTMHALI.
OrthoDBiEOG7Z3F50.
PhylomeDBiO88876.
TreeFamiTF312837.

Enzyme and pathway databases

ReactomeiREACT_280434. The retinoid cycle in cones (daylight vision).
REACT_310468. RA biosynthesis pathway.

Miscellaneous databases

NextBioi297657.
PROiO88876.
SOURCEiSearch...

Gene expression databases

BgeeiO88876.
CleanExiMM_DHRS3.
ExpressionAtlasiO88876. baseline and differential.
GenevestigatoriO88876.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a novel short-chain dehydrogenase/reductase that reduces all-trans-retinal."
    Haeseleer F., Huang J., Lebioda L., Saari J.C., Palczewski K.
    J. Biol. Chem. 273:21790-21799(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Retina.
  2. "Bmp-2 downstream targets in mesenchymal development identified by subtractive cloning from recombinant mesenchymal progenitors (C3H10T1/2)."
    Baechner D., Ahrens M., Schroeder D., Hoffmann A., Lauber J., Betat N., Steinert P., Flohe L., Gross G.
    Dev. Dyn. 213:398-411(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Mesenchymal cell.
  3. "Structure of retinal short-chain dehydrogenase/reductase retSDR1 gene."
    Haeseleer F., Palczewski K.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
    Tissue: Retina.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow and Mesonephros.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Colon, Kidney and Mammary gland.

Entry informationi

Entry nameiDHRS3_MOUSE
AccessioniPrimary (citable) accession number: O88876
Secondary accession number(s): Q3UAD1
, Q91WR0, Q91XC3, Q922A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2004
Last sequence update: May 9, 2004
Last modified: March 31, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.