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O88874

- CCNK_MOUSE

UniProt

O88874 - CCNK_MOUSE

Protein

Cyclin-K

Gene

Ccnk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).1 Publication

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase activity Source: MGI
    2. RNA polymerase II carboxy-terminal domain kinase activity Source: MGI

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of cell cycle arrest Source: MGI
    4. protein phosphorylation Source: GOC
    5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-K
    Gene namesi
    Name:Ccnk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1276106. Ccnk.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Complete embryonic lethality. No homozygous embryos are detected at any stage of embryonic development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Cyclin-KPRO_0000080479Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei325 – 3251PhosphoserineBy similarity
    Modified residuei341 – 3411Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO88874.
    PaxDbiO88874.
    PRIDEiO88874.

    PTM databases

    PhosphoSiteiO88874.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Highest levels in testis. Present throughout the seminiferous epithelium. Also highly expressed in the developing oocyte.

    Developmental stagei

    Found in 12.5 dpc embryo with abundant expression in eye (retinal pigment epithelium) and ear (cochlea, crista ampullaris of the semicircular canals).

    Gene expression databases

    CleanExiMM_CCNK.
    GenevestigatoriO88874.

    Interactioni

    Subunit structurei

    Regulatory subunit of cyclin-dependent kinases. Identified in a complex with a kinase and the RNA polymerase II holoenzyme. Interacts with POLR2A. Interacts with CDK12 and CDK13. Its interaction with CDK9 is controversial.1 Publication

    Protein-protein interaction databases

    IntActiO88874. 1 interaction.
    MINTiMINT-4090092.

    Structurei

    3D structure databases

    ProteinModelPortaliO88874.
    SMRiO88874. Positions 14-267.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyclin family. Cyclin C subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5333.
    HOGENOMiHOG000220828.
    HOVERGENiHBG050836.

    Family and domain databases

    Gene3Di1.10.472.10. 1 hit.
    InterProiIPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    [Graphical view]
    PANTHERiPTHR10026. PTHR10026. 1 hit.
    PfamiPF00134. Cyclin_N. 1 hit.
    [Graphical view]
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O88874-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKENKENSSP SVTSANLDHT KPCWYWDKKD LAHTPSQLEG LDPATEARYR    50
    REGARFIFDV GTRLGLHYDT LATGIIYFHR FYMFHSFKQF PRYVTGACCL 100
    FLAGKVEETP KKCKDIIKTA RSLLNDVQFG QFGDDPKEEV MVLERILLQT 150
    IKFDLQVEHP YQFLLKYAKQ LKGDKNKIQK LVQMAWTFVN DSLCTTLSLQ 200
    WEPEIIAVAV MYLAGRLCKF EIQEWTSKPM YRRWWEQFVQ DVPVDVLEDI 250
    CHQILDLYSQ GKQQMPHHTP HQLQQPPSLQ PTPQVPQGPQ SQPSQGSEAA 300
    QPPQKDSQQS AQQQQQQAQQ PKKPSPQPSP PRQAKRAVVV SPKEENKATE 350
    PPPPPKIPKL EATHPPLPPA HPPPDRKPPL APALGEAEAT GPVETSDLPK 400
    VQIPPPAHPA PVHQPPPLPH RPPPPPPSSY MTGMSTTSSY MSGEGYQSLQ 450
    SMMKTEGPSY GALPPASFPP PTIPPPTPGY PPPPPTYNPN FPPPPPRLPP 500
    THAVPPHPPP GLGLPPASYP PPAVPPGGQP PVPPPIPPPG MPPVGGLGRA 550
    AWMR 554
    Length:554
    Mass (Da):61,363
    Last modified:July 27, 2011 - v3
    Checksum:iDB2CE7B821834C46
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731T → N in AAH27297. (PubMed:15489334)Curated
    Sequence conflicti202 – 2021E → K in AAD09979. (PubMed:9632813)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027297 mRNA. Translation: AAH27297.1.
    AC152059 Genomic DNA. No translation available.
    AF060517 mRNA. Translation: AAD09979.1.
    UniGeneiMm.474441.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC027297 mRNA. Translation: AAH27297.1 .
    AC152059 Genomic DNA. No translation available.
    AF060517 mRNA. Translation: AAD09979.1 .
    UniGenei Mm.474441.

    3D structure databases

    ProteinModelPortali O88874.
    SMRi O88874. Positions 14-267.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O88874. 1 interaction.
    MINTi MINT-4090092.

    PTM databases

    PhosphoSitei O88874.

    Proteomic databases

    MaxQBi O88874.
    PaxDbi O88874.
    PRIDEi O88874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:1276106. Ccnk.

    Phylogenomic databases

    eggNOGi COG5333.
    HOGENOMi HOG000220828.
    HOVERGENi HBG050836.

    Miscellaneous databases

    PROi O88874.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CCNK.
    Genevestigatori O88874.

    Family and domain databases

    Gene3Di 1.10.472.10. 1 hit.
    InterProi IPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    [Graphical view ]
    PANTHERi PTHR10026. PTHR10026. 1 hit.
    Pfami PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity."
      Edwards M.C., Wong C., Elledge S.J.
      Mol. Cell. Biol. 18:4291-4300(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-222.
      Tissue: Testis.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes."
      Blazek D., Kohoutek J., Bartholomeeusen K., Johansen E., Hulinkova P., Luo Z., Cimermancic P., Ule J., Peterlin B.M.
      Genes Dev. 25:2158-2172(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCCNK_MOUSE
    AccessioniPrimary (citable) accession number: O88874
    Secondary accession number(s): E9QKT7, Q8R068
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3