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Protein

Cyclin-K

Gene

Ccnk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).1 Publication

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase activity Source: MGI
  2. protein kinase binding Source: MGI
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: MGI

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. cellular response to DNA damage stimulus Source: MGI
  3. in utero embryonic development Source: MGI
  4. mitotic nuclear division Source: UniProtKB-KW
  5. negative regulation of cell cycle arrest Source: MGI
  6. protein phosphorylation Source: MGI
  7. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-K
Gene namesi
Name:Ccnk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1276106. Ccnk.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cyclin K-CDK12 complex Source: MGI
  2. cyclin K-CDK13 complex Source: MGI
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Complete embryonic lethality. No homozygous embryos are detected at any stage of embryonic development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Cyclin-KPRO_0000080479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251PhosphoserineBy similarity
Modified residuei341 – 3411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88874.
PaxDbiO88874.
PRIDEiO88874.

PTM databases

PhosphoSiteiO88874.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highest levels in testis. Present throughout the seminiferous epithelium. Also highly expressed in the developing oocyte.

Developmental stagei

Found in 12.5 dpc embryo with abundant expression in eye (retinal pigment epithelium) and ear (cochlea, crista ampullaris of the semicircular canals).

Gene expression databases

CleanExiMM_CCNK.
GenevestigatoriO88874.

Interactioni

Subunit structurei

Regulatory subunit of cyclin-dependent kinases. Identified in a complex with a kinase and the RNA polymerase II holoenzyme. Interacts with POLR2A. Interacts with CDK12 and CDK13. Its interaction with CDK9 is controversial.1 Publication

Protein-protein interaction databases

IntActiO88874. 1 interaction.
MINTiMINT-4090092.

Structurei

3D structure databases

ProteinModelPortaliO88874.
SMRiO88874. Positions 14-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin C subfamily.Curated

Phylogenomic databases

eggNOGiCOG5333.
HOGENOMiHOG000220828.
HOVERGENiHBG050836.
InParanoidiO88874.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10026. PTHR10026. 1 hit.
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.

Sequencei

Sequence statusi: Complete.

O88874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKENKENSSP SVTSANLDHT KPCWYWDKKD LAHTPSQLEG LDPATEARYR
60 70 80 90 100
REGARFIFDV GTRLGLHYDT LATGIIYFHR FYMFHSFKQF PRYVTGACCL
110 120 130 140 150
FLAGKVEETP KKCKDIIKTA RSLLNDVQFG QFGDDPKEEV MVLERILLQT
160 170 180 190 200
IKFDLQVEHP YQFLLKYAKQ LKGDKNKIQK LVQMAWTFVN DSLCTTLSLQ
210 220 230 240 250
WEPEIIAVAV MYLAGRLCKF EIQEWTSKPM YRRWWEQFVQ DVPVDVLEDI
260 270 280 290 300
CHQILDLYSQ GKQQMPHHTP HQLQQPPSLQ PTPQVPQGPQ SQPSQGSEAA
310 320 330 340 350
QPPQKDSQQS AQQQQQQAQQ PKKPSPQPSP PRQAKRAVVV SPKEENKATE
360 370 380 390 400
PPPPPKIPKL EATHPPLPPA HPPPDRKPPL APALGEAEAT GPVETSDLPK
410 420 430 440 450
VQIPPPAHPA PVHQPPPLPH RPPPPPPSSY MTGMSTTSSY MSGEGYQSLQ
460 470 480 490 500
SMMKTEGPSY GALPPASFPP PTIPPPTPGY PPPPPTYNPN FPPPPPRLPP
510 520 530 540 550
THAVPPHPPP GLGLPPASYP PPAVPPGGQP PVPPPIPPPG MPPVGGLGRA

AWMR
Length:554
Mass (Da):61,363
Last modified:July 27, 2011 - v3
Checksum:iDB2CE7B821834C46
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731T → N in AAH27297 (PubMed:15489334).Curated
Sequence conflicti202 – 2021E → K in AAD09979 (PubMed:9632813).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027297 mRNA. Translation: AAH27297.1.
AC152059 Genomic DNA. No translation available.
AF060517 mRNA. Translation: AAD09979.1.
UniGeneiMm.474441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027297 mRNA. Translation: AAH27297.1.
AC152059 Genomic DNA. No translation available.
AF060517 mRNA. Translation: AAD09979.1.
UniGeneiMm.474441.

3D structure databases

ProteinModelPortaliO88874.
SMRiO88874. Positions 14-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88874. 1 interaction.
MINTiMINT-4090092.

PTM databases

PhosphoSiteiO88874.

Proteomic databases

MaxQBiO88874.
PaxDbiO88874.
PRIDEiO88874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1276106. Ccnk.

Phylogenomic databases

eggNOGiCOG5333.
HOGENOMiHOG000220828.
HOVERGENiHBG050836.
InParanoidiO88874.

Miscellaneous databases

ChiTaRSiCcnk. mouse.
PROiO88874.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CCNK.
GenevestigatoriO88874.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10026. PTHR10026. 1 hit.
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity."
    Edwards M.C., Wong C., Elledge S.J.
    Mol. Cell. Biol. 18:4291-4300(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-222.
    Tissue: Testis.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes."
    Blazek D., Kohoutek J., Bartholomeeusen K., Johansen E., Hulinkova P., Luo Z., Cimermancic P., Ule J., Peterlin B.M.
    Genes Dev. 25:2158-2172(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCCNK_MOUSE
AccessioniPrimary (citable) accession number: O88874
Secondary accession number(s): E9QKT7, Q8R068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.