Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O88874 (CCNK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-K
Gene names
Name:Ccnk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A). Ref.5

Subunit structure

Regulatory subunit of cyclin-dependent kinases. Identified in a complex with a kinase and the RNA polymerase II holoenzyme. Interacts with POLR2A. Interacts with CDK12 and CDK13. Its interaction with CDK9 is controversial. Ref.5

Subcellular location

Nucleus Probable Ref.5.

Tissue specificity

Ubiquitously expressed. Highest levels in testis. Present throughout the seminiferous epithelium. Also highly expressed in the developing oocyte.

Developmental stage

Found in 12.5 dpc embryo with abundant expression in eye (retinal pigment epithelium) and ear (cochlea, crista ampullaris of the semicircular canals).

Disruption phenotype

Complete embryonic lethality. No homozygous embryos are detected at any stage of embryonic development. Ref.5

Sequence similarities

Belongs to the cyclin family. Cyclin C subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Cyclin-K
PRO_0000080479

Amino acid modifications

Modified residue3251Phosphoserine By similarity
Modified residue3411Phosphoserine Ref.4

Experimental info

Sequence conflict731T → N in AAH27297. Ref.1
Sequence conflict2021E → K in AAD09979. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O88874 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: DB2CE7B821834C46

FASTA55461,363
        10         20         30         40         50         60 
MKENKENSSP SVTSANLDHT KPCWYWDKKD LAHTPSQLEG LDPATEARYR REGARFIFDV 

        70         80         90        100        110        120 
GTRLGLHYDT LATGIIYFHR FYMFHSFKQF PRYVTGACCL FLAGKVEETP KKCKDIIKTA 

       130        140        150        160        170        180 
RSLLNDVQFG QFGDDPKEEV MVLERILLQT IKFDLQVEHP YQFLLKYAKQ LKGDKNKIQK 

       190        200        210        220        230        240 
LVQMAWTFVN DSLCTTLSLQ WEPEIIAVAV MYLAGRLCKF EIQEWTSKPM YRRWWEQFVQ 

       250        260        270        280        290        300 
DVPVDVLEDI CHQILDLYSQ GKQQMPHHTP HQLQQPPSLQ PTPQVPQGPQ SQPSQGSEAA 

       310        320        330        340        350        360 
QPPQKDSQQS AQQQQQQAQQ PKKPSPQPSP PRQAKRAVVV SPKEENKATE PPPPPKIPKL 

       370        380        390        400        410        420 
EATHPPLPPA HPPPDRKPPL APALGEAEAT GPVETSDLPK VQIPPPAHPA PVHQPPPLPH 

       430        440        450        460        470        480 
RPPPPPPSSY MTGMSTTSSY MSGEGYQSLQ SMMKTEGPSY GALPPASFPP PTIPPPTPGY 

       490        500        510        520        530        540 
PPPPPTYNPN FPPPPPRLPP THAVPPHPPP GLGLPPASYP PPAVPPGGQP PVPPPIPPPG 

       550 
MPPVGGLGRA AWMR 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity."
Edwards M.C., Wong C., Elledge S.J.
Mol. Cell. Biol. 18:4291-4300(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-222.
Tissue: Testis.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes."
Blazek D., Kohoutek J., Bartholomeeusen K., Johansen E., Hulinkova P., Luo Z., Cimermancic P., Ule J., Peterlin B.M.
Genes Dev. 25:2158-2172(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC027297 mRNA. Translation: AAH27297.1.
AC152059 Genomic DNA. No translation available.
AF060517 mRNA. Translation: AAD09979.1.
UniGeneMm.474441.

3D structure databases

ProteinModelPortalO88874.
SMRO88874. Positions 14-267.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO88874. 1 interaction.
MINTMINT-4090092.

PTM databases

PhosphoSiteO88874.

Proteomic databases

MaxQBO88874.
PaxDbO88874.
PRIDEO88874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1276106. Ccnk.

Phylogenomic databases

eggNOGCOG5333.
HOGENOMHOG000220828.
HOVERGENHBG050836.

Gene expression databases

CleanExMM_CCNK.
GenevestigatorO88874.

Family and domain databases

Gene3D1.10.472.10. 1 hit.
InterProIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERPTHR10026. PTHR10026. 1 hit.
PfamPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
ProtoNetSearch...

Other

PROO88874.
SOURCESearch...

Entry information

Entry nameCCNK_MOUSE
AccessionPrimary (citable) accession number: O88874
Secondary accession number(s): E9QKT7, Q8R068
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot