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O88871

- GABR2_RAT

UniProt

O88871 - GABR2_RAT

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Protein

Gamma-aminobutyric acid type B receptor subunit 2

Gene
Gabbr2, Gpr51
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

GO - Molecular functioni

  1. G-protein coupled GABA receptor activity Source: RGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. gamma-aminobutyric acid signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 2
Short name:
GABA-B receptor 2
Short name:
GABA-B-R2
Short name:
GABA-BR2
Short name:
GABABR2
Short name:
Gb2
Alternative name(s):
G-protein coupled receptor 51
Gene namesi
Name:Gabbr2
Synonyms:Gpr51
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619864. Gabbr2.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 482442Extracellular Reviewed predictionAdd
BLAST
Transmembranei483 – 50321Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini504 – 52118Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei522 – 54221Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini543 – 5508Extracellular Reviewed prediction
Transmembranei551 – 57121Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini572 – 59625Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei597 – 61721Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini618 – 65336Extracellular Reviewed predictionAdd
BLAST
Transmembranei654 – 67421Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini675 – 69016Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei691 – 71121Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini712 – 7198Extracellular Reviewed prediction
Transmembranei720 – 74021Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini741 – 940200Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. G-protein coupled receptor heterodimeric complex Source: UniProtKB
  3. integral component of membrane Source: RGD
  4. integral component of plasma membrane Source: UniProtKB
  5. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040 Reviewed predictionAdd
BLAST
Chaini41 – 940900Gamma-aminobutyric acid type B receptor subunit 2PRO_0000012953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi107 ↔ 134 By similarity
Disulfide bondi236 ↔ 265 By similarity
Disulfide bondi264 ↔ 301 By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi388 – 3881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi403 – 4031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi452 – 4521N-linked (GlcNAc...) Reviewed prediction
Modified residuei883 – 8831Phosphoserine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88871.
PRIDEiO88871.

PTM databases

PhosphoSiteiO88871.

Expressioni

Tissue specificityi

Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression is seen in cerebellum.2 Publications

Developmental stagei

Abundant in brain cortex and cerebellum throughout postnatal development whereas its expression in spinal cord gradually decreases.

Gene expression databases

ArrayExpressiO88871.
GenevestigatoriO88871.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cacna1dP277326EBI-7090239,EBI-8072674
Gabbr1Q9Z0U48EBI-7090239,EBI-7090268

Protein-protein interaction databases

BioGridi249797. 2 interactions.
IntActiO88871. 3 interactions.
MINTiMINT-1889821.

Structurei

3D structure databases

ProteinModelPortaliO88871.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili780 – 81839 Reviewed predictionAdd
BLAST

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG273984.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiO88871.
KOiK04615.
PhylomeDBiO88871.
TreeFamiTF313965.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88871-1 [UniParc]FASTAAdd to Basket

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MASPPSSGQP RPPPPPPPPA RLLLPLLLSL LLWLAPGAWG WTRGAPRPPP    50
SSPPLSIMGL MPLTKEVAKG SIGRGVLPAV ELAIEQIRNE SLLRPYFLDL 100
RLYDTECDNA KGLKAFYDAI KYGPNHLMVF GGVCPSVTSI IAESLQGWNL 150
VQLSFAATTP VLADKKKYPY FFRTVPSDNA VNPAILKLLK HFRWRRVGTL 200
TQDVQRFSEV RNDLTGVLYG EDIEISDTES FSNDPCTSVK KLKGNDVRII 250
LGQFDQNMAA KVFCCAFEES MFGSKYQWII PGWYEPAWWE QVHVEANSSR 300
CLRRSLLAAM EGYIGVDFEP LSSKQIKTIS GKTPQQFERE YNSKRSGVGP 350
SKFHGYAYDG IWVIAKTLQR AMETLHASSR HQRIQDFNYT DHTLGKIILN 400
AMNETNFFGV TGQVVFRNGE RMGTIKFTQF QDSREVKVGE YNAVADTLEI 450
INDTIRFQGS EPPKDKTIIL EQLRKISLPL YSILSALTIL GMIMASAFLF 500
FNIKNRNQKL IKMSSPYMNN LIILGGMLSY ASIFLFGLDG SFVSEKTFET 550
LCTVRTWILT VGYTTAFGAM FAKTWRVHAI FKNVKMKKKI IKDQKLLVIV 600
GGMLLIDLCI LICWQAVDPL RRTVERYSME PDPAGRDISI RPLLEHCENT 650
HMTIWLGIVY AYKGLLMLFG CFLAWETRNV SIPALNDSKY IGMSVYNVGI 700
MCIIGAAVSF LTRDQPNVQF CIVALVIIFC STITLCLVFV PKLITLRTNP 750
DAATQNRRFQ FTQNQKKEDS KTSTSVTSVN QASTSRLEGL QSENHRLRMK 800
ITELDKDLEE VTMQLQDTPE KTTYIKQNHY QELNDILSLG NFTESTDGGK 850
AILKNHLDQN PQLQWNTTEP SRTCKDPIED INSPEHIQRR LSLQLPILHH 900
AYLPSIGGVD ASCVSPCVSP TASPRHRHVP PSFRVMVSGL 940
Length:940
Mass (Da):105,751
Last modified:January 11, 2001 - v2
Checksum:i77BB42D833C7505D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191P → PP.1 Publication
Natural varianti19 – 191P → R.
Natural varianti337 – 3371F → Y.1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti343 – 3431S → T in CAA09592. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF074482 mRNA. Translation: AAD03335.1.
AJ011318 Genomic DNA. Translation: CAA09592.1.
AF058795 mRNA. Translation: AAC63994.1.
AF109405 mRNA. Translation: AAD03338.1.
RefSeqiNP_113990.1. NM_031802.1.
UniGeneiRn.162814.

Genome annotation databases

GeneIDi83633.
KEGGirno:83633.
UCSCiRGD:619864. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF074482 mRNA. Translation: AAD03335.1 .
AJ011318 Genomic DNA. Translation: CAA09592.1 .
AF058795 mRNA. Translation: AAC63994.1 .
AF109405 mRNA. Translation: AAD03338.1 .
RefSeqi NP_113990.1. NM_031802.1.
UniGenei Rn.162814.

3D structure databases

ProteinModelPortali O88871.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249797. 2 interactions.
IntActi O88871. 3 interactions.
MINTi MINT-1889821.

Chemistry

BindingDBi O88871.
ChEMBLi CHEMBL2111474.
GuidetoPHARMACOLOGYi 241.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei O88871.

Proteomic databases

PaxDbi O88871.
PRIDEi O88871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 83633.
KEGGi rno:83633.
UCSCi RGD:619864. rat.

Organism-specific databases

CTDi 9568.
RGDi 619864. Gabbr2.

Phylogenomic databases

eggNOGi NOG273984.
HOGENOMi HOG000112688.
HOVERGENi HBG080355.
InParanoidi O88871.
KOi K04615.
PhylomeDBi O88871.
TreeFami TF313965.

Miscellaneous databases

NextBioi 616227.
PROi O88871.

Gene expression databases

ArrayExpressi O88871.
Genevestigatori O88871.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view ]
PANTHERi PTHR10519. PTHR10519. 1 hit.
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view ]
PRINTSi PR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH GABBR1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain cortex and Cerebellum.
  3. "Distribution of the GABA(B) receptor subunit gb2 in rat CNS."
    Clark J.A., Mezey E., Lam A.S., Bonner T.I.
    Brain Res. 860:41-52(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain cortex.
  4. Borowsky B., Laz T., Gerald C.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  5. "Role of heteromer formation in GABAB receptor function."
    Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C.
    Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GABBR1, VARIANTS PRO-19 INS AND TYR-337.
    Tissue: Hippocampus.
  6. "The metabotropic GABAB receptor directly interacts with the activating transcription factor 4."
    Nehring R.B., Horikawa H.P.M., El Far O., Kneussel M., Brandstatter J.H., Stamm S., Wischmeyer E., Betz H., Karschin A.
    J. Biol. Chem. 275:35185-35191(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATF4.

Entry informationi

Entry nameiGABR2_RAT
AccessioniPrimary (citable) accession number: O88871
Secondary accession number(s): Q9JK36, Q9QWU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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