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O88871

- GABR2_RAT

UniProt

O88871 - GABR2_RAT

Protein

Gamma-aminobutyric acid type B receptor subunit 2

Gene

Gabbr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

    GO - Molecular functioni

    1. G-protein coupled GABA receptor activity Source: RGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. gamma-aminobutyric acid signaling pathway Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid type B receptor subunit 2
    Short name:
    GABA-B receptor 2
    Short name:
    GABA-B-R2
    Short name:
    GABA-BR2
    Short name:
    GABABR2
    Short name:
    Gb2
    Alternative name(s):
    G-protein coupled receptor 51
    Gene namesi
    Name:Gabbr2
    Synonyms:Gpr51
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619864. Gabbr2.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. G-protein coupled receptor heterodimeric complex Source: UniProtKB
    3. integral component of membrane Source: RGD
    4. integral component of plasma membrane Source: UniProtKB
    5. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4040Sequence AnalysisAdd
    BLAST
    Chaini41 – 940900Gamma-aminobutyric acid type B receptor subunit 2PRO_0000012953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi107 ↔ 134By similarity
    Disulfide bondi236 ↔ 265By similarity
    Disulfide bondi264 ↔ 301By similarity
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
    Modified residuei883 – 8831PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiO88871.
    PRIDEiO88871.

    PTM databases

    PhosphoSiteiO88871.

    Expressioni

    Tissue specificityi

    Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression is seen in cerebellum.2 Publications

    Developmental stagei

    Abundant in brain cortex and cerebellum throughout postnatal development whereas its expression in spinal cord gradually decreases.

    Gene expression databases

    ArrayExpressiO88871.
    GenevestigatoriO88871.

    Interactioni

    Subunit structurei

    Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cacna1dP277326EBI-7090239,EBI-8072674
    Gabbr1Q9Z0U48EBI-7090239,EBI-7090268

    Protein-protein interaction databases

    BioGridi249797. 2 interactions.
    IntActiO88871. 3 interactions.
    MINTiMINT-1889821.

    Structurei

    3D structure databases

    ProteinModelPortaliO88871.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini41 – 482442ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini504 – 52118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini543 – 5508ExtracellularSequence Analysis
    Topological domaini572 – 59625CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini618 – 65336ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini675 – 69016CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini712 – 7198ExtracellularSequence Analysis
    Topological domaini741 – 940200CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei483 – 50321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei522 – 54221Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei551 – 57121Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei597 – 61721Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei654 – 67421Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei691 – 71121Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei720 – 74021Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili780 – 81839Sequence AnalysisAdd
    BLAST

    Domaini

    Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG273984.
    HOGENOMiHOG000112688.
    HOVERGENiHBG080355.
    InParanoidiO88871.
    KOiK04615.
    PhylomeDBiO88871.
    TreeFamiTF313965.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR002455. GPCR3_GABA-B.
    IPR000337. GPCR_3.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR002457. GPCR_3_GABA_rcpt_B2.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PANTHERiPTHR10519. PTHR10519. 1 hit.
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    [Graphical view]
    PRINTSiPR01178. GABAB2RECPTR.
    PR00248. GPCRMGR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88871-1 [UniParc]FASTAAdd to Basket

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    MASPPSSGQP RPPPPPPPPA RLLLPLLLSL LLWLAPGAWG WTRGAPRPPP    50
    SSPPLSIMGL MPLTKEVAKG SIGRGVLPAV ELAIEQIRNE SLLRPYFLDL 100
    RLYDTECDNA KGLKAFYDAI KYGPNHLMVF GGVCPSVTSI IAESLQGWNL 150
    VQLSFAATTP VLADKKKYPY FFRTVPSDNA VNPAILKLLK HFRWRRVGTL 200
    TQDVQRFSEV RNDLTGVLYG EDIEISDTES FSNDPCTSVK KLKGNDVRII 250
    LGQFDQNMAA KVFCCAFEES MFGSKYQWII PGWYEPAWWE QVHVEANSSR 300
    CLRRSLLAAM EGYIGVDFEP LSSKQIKTIS GKTPQQFERE YNSKRSGVGP 350
    SKFHGYAYDG IWVIAKTLQR AMETLHASSR HQRIQDFNYT DHTLGKIILN 400
    AMNETNFFGV TGQVVFRNGE RMGTIKFTQF QDSREVKVGE YNAVADTLEI 450
    INDTIRFQGS EPPKDKTIIL EQLRKISLPL YSILSALTIL GMIMASAFLF 500
    FNIKNRNQKL IKMSSPYMNN LIILGGMLSY ASIFLFGLDG SFVSEKTFET 550
    LCTVRTWILT VGYTTAFGAM FAKTWRVHAI FKNVKMKKKI IKDQKLLVIV 600
    GGMLLIDLCI LICWQAVDPL RRTVERYSME PDPAGRDISI RPLLEHCENT 650
    HMTIWLGIVY AYKGLLMLFG CFLAWETRNV SIPALNDSKY IGMSVYNVGI 700
    MCIIGAAVSF LTRDQPNVQF CIVALVIIFC STITLCLVFV PKLITLRTNP 750
    DAATQNRRFQ FTQNQKKEDS KTSTSVTSVN QASTSRLEGL QSENHRLRMK 800
    ITELDKDLEE VTMQLQDTPE KTTYIKQNHY QELNDILSLG NFTESTDGGK 850
    AILKNHLDQN PQLQWNTTEP SRTCKDPIED INSPEHIQRR LSLQLPILHH 900
    AYLPSIGGVD ASCVSPCVSP TASPRHRHVP PSFRVMVSGL 940
    Length:940
    Mass (Da):105,751
    Last modified:January 11, 2001 - v2
    Checksum:i77BB42D833C7505D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti343 – 3431S → T in CAA09592. (PubMed:9872317)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti19 – 191P → PP.1 Publication
    Natural varianti19 – 191P → R.
    Natural varianti337 – 3371F → Y.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF074482 mRNA. Translation: AAD03335.1.
    AJ011318 Genomic DNA. Translation: CAA09592.1.
    AF058795 mRNA. Translation: AAC63994.1.
    AF109405 mRNA. Translation: AAD03338.1.
    RefSeqiNP_113990.1. NM_031802.1.
    UniGeneiRn.162814.

    Genome annotation databases

    GeneIDi83633.
    KEGGirno:83633.
    UCSCiRGD:619864. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF074482 mRNA. Translation: AAD03335.1 .
    AJ011318 Genomic DNA. Translation: CAA09592.1 .
    AF058795 mRNA. Translation: AAC63994.1 .
    AF109405 mRNA. Translation: AAD03338.1 .
    RefSeqi NP_113990.1. NM_031802.1.
    UniGenei Rn.162814.

    3D structure databases

    ProteinModelPortali O88871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249797. 2 interactions.
    IntActi O88871. 3 interactions.
    MINTi MINT-1889821.

    Chemistry

    BindingDBi O88871.
    ChEMBLi CHEMBL2111474.
    GuidetoPHARMACOLOGYi 241.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei O88871.

    Proteomic databases

    PaxDbi O88871.
    PRIDEi O88871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 83633.
    KEGGi rno:83633.
    UCSCi RGD:619864. rat.

    Organism-specific databases

    CTDi 9568.
    RGDi 619864. Gabbr2.

    Phylogenomic databases

    eggNOGi NOG273984.
    HOGENOMi HOG000112688.
    HOVERGENi HBG080355.
    InParanoidi O88871.
    KOi K04615.
    PhylomeDBi O88871.
    TreeFami TF313965.

    Miscellaneous databases

    NextBioi 616227.
    PROi O88871.

    Gene expression databases

    ArrayExpressi O88871.
    Genevestigatori O88871.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR002455. GPCR3_GABA-B.
    IPR000337. GPCR_3.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR002457. GPCR_3_GABA_rcpt_B2.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    PANTHERi PTHR10519. PTHR10519. 1 hit.
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    [Graphical view ]
    PRINTSi PR01178. GABAB2RECPTR.
    PR00248. GPCRMGR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hypothalamus.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH GABBR1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Brain cortex and Cerebellum.
    3. "Distribution of the GABA(B) receptor subunit gb2 in rat CNS."
      Clark J.A., Mezey E., Lam A.S., Bonner T.I.
      Brain Res. 860:41-52(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Brain cortex.
    4. Borowsky B., Laz T., Gerald C.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hypothalamus.
    5. "Role of heteromer formation in GABAB receptor function."
      Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C.
      Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GABBR1, VARIANTS PRO-19 INS AND TYR-337.
      Tissue: Hippocampus.
    6. "The metabotropic GABAB receptor directly interacts with the activating transcription factor 4."
      Nehring R.B., Horikawa H.P.M., El Far O., Kneussel M., Brandstatter J.H., Stamm S., Wischmeyer E., Betz H., Karschin A.
      J. Biol. Chem. 275:35185-35191(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATF4.

    Entry informationi

    Entry nameiGABR2_RAT
    AccessioniPrimary (citable) accession number: O88871
    Secondary accession number(s): Q9JK36, Q9QWU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3