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O88871

- GABR2_RAT

UniProt

O88871 - GABR2_RAT

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Protein

Gamma-aminobutyric acid type B receptor subunit 2

Gene

Gabbr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

GO - Molecular functioni

  1. G-protein coupled GABA receptor activity Source: RGD

GO - Biological processi

  1. gamma-aminobutyric acid signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 2
Short name:
GABA-B receptor 2
Short name:
GABA-B-R2
Short name:
GABA-BR2
Short name:
GABABR2
Short name:
Gb2
Alternative name(s):
G-protein coupled receptor 51
Gene namesi
Name:Gabbr2
Synonyms:Gpr51
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619864. Gabbr2.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 482442ExtracellularSequence AnalysisAdd
BLAST
Transmembranei483 – 50321Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini504 – 52118CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei522 – 54221Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini543 – 5508ExtracellularSequence Analysis
Transmembranei551 – 57121Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini572 – 59625CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei597 – 61721Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini618 – 65336ExtracellularSequence AnalysisAdd
BLAST
Transmembranei654 – 67421Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini675 – 69016CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei691 – 71121Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini712 – 7198ExtracellularSequence Analysis
Transmembranei720 – 74021Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini741 – 940200CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. G-protein coupled receptor heterodimeric complex Source: UniProtKB
  3. integral component of membrane Source: RGD
  4. integral component of plasma membrane Source: UniProtKB
  5. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence AnalysisAdd
BLAST
Chaini41 – 940900Gamma-aminobutyric acid type B receptor subunit 2PRO_0000012953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi107 ↔ 134By similarity
Disulfide bondi236 ↔ 265By similarity
Disulfide bondi264 ↔ 301By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
Modified residuei883 – 8831PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88871.
PRIDEiO88871.

PTM databases

PhosphoSiteiO88871.

Expressioni

Tissue specificityi

Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression is seen in cerebellum.2 Publications

Developmental stagei

Abundant in brain cortex and cerebellum throughout postnatal development whereas its expression in spinal cord gradually decreases.

Gene expression databases

GenevestigatoriO88871.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cacna1dP277326EBI-7090239,EBI-8072674
Gabbr1Q9Z0U48EBI-7090239,EBI-7090268

Protein-protein interaction databases

BioGridi249797. 4 interactions.
IntActiO88871. 3 interactions.
MINTiMINT-1889821.

Structurei

3D structure databases

ProteinModelPortaliO88871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili780 – 81839Sequence AnalysisAdd
BLAST

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG273984.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiO88871.
KOiK04615.
PhylomeDBiO88871.
TreeFamiTF313965.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88871-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPPSSGQP RPPPPPPPPA RLLLPLLLSL LLWLAPGAWG WTRGAPRPPP
60 70 80 90 100
SSPPLSIMGL MPLTKEVAKG SIGRGVLPAV ELAIEQIRNE SLLRPYFLDL
110 120 130 140 150
RLYDTECDNA KGLKAFYDAI KYGPNHLMVF GGVCPSVTSI IAESLQGWNL
160 170 180 190 200
VQLSFAATTP VLADKKKYPY FFRTVPSDNA VNPAILKLLK HFRWRRVGTL
210 220 230 240 250
TQDVQRFSEV RNDLTGVLYG EDIEISDTES FSNDPCTSVK KLKGNDVRII
260 270 280 290 300
LGQFDQNMAA KVFCCAFEES MFGSKYQWII PGWYEPAWWE QVHVEANSSR
310 320 330 340 350
CLRRSLLAAM EGYIGVDFEP LSSKQIKTIS GKTPQQFERE YNSKRSGVGP
360 370 380 390 400
SKFHGYAYDG IWVIAKTLQR AMETLHASSR HQRIQDFNYT DHTLGKIILN
410 420 430 440 450
AMNETNFFGV TGQVVFRNGE RMGTIKFTQF QDSREVKVGE YNAVADTLEI
460 470 480 490 500
INDTIRFQGS EPPKDKTIIL EQLRKISLPL YSILSALTIL GMIMASAFLF
510 520 530 540 550
FNIKNRNQKL IKMSSPYMNN LIILGGMLSY ASIFLFGLDG SFVSEKTFET
560 570 580 590 600
LCTVRTWILT VGYTTAFGAM FAKTWRVHAI FKNVKMKKKI IKDQKLLVIV
610 620 630 640 650
GGMLLIDLCI LICWQAVDPL RRTVERYSME PDPAGRDISI RPLLEHCENT
660 670 680 690 700
HMTIWLGIVY AYKGLLMLFG CFLAWETRNV SIPALNDSKY IGMSVYNVGI
710 720 730 740 750
MCIIGAAVSF LTRDQPNVQF CIVALVIIFC STITLCLVFV PKLITLRTNP
760 770 780 790 800
DAATQNRRFQ FTQNQKKEDS KTSTSVTSVN QASTSRLEGL QSENHRLRMK
810 820 830 840 850
ITELDKDLEE VTMQLQDTPE KTTYIKQNHY QELNDILSLG NFTESTDGGK
860 870 880 890 900
AILKNHLDQN PQLQWNTTEP SRTCKDPIED INSPEHIQRR LSLQLPILHH
910 920 930 940
AYLPSIGGVD ASCVSPCVSP TASPRHRHVP PSFRVMVSGL
Length:940
Mass (Da):105,751
Last modified:January 11, 2001 - v2
Checksum:i77BB42D833C7505D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti343 – 3431S → T in CAA09592. (PubMed:9872317)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191P → PP.1 Publication
Natural varianti19 – 191P → R.
Natural varianti337 – 3371F → Y.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074482 mRNA. Translation: AAD03335.1.
AJ011318 Genomic DNA. Translation: CAA09592.1.
AF058795 mRNA. Translation: AAC63994.1.
AF109405 mRNA. Translation: AAD03338.1.
RefSeqiNP_113990.1. NM_031802.1.
UniGeneiRn.162814.

Genome annotation databases

GeneIDi83633.
KEGGirno:83633.
UCSCiRGD:619864. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074482 mRNA. Translation: AAD03335.1 .
AJ011318 Genomic DNA. Translation: CAA09592.1 .
AF058795 mRNA. Translation: AAC63994.1 .
AF109405 mRNA. Translation: AAD03338.1 .
RefSeqi NP_113990.1. NM_031802.1.
UniGenei Rn.162814.

3D structure databases

ProteinModelPortali O88871.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249797. 4 interactions.
IntActi O88871. 3 interactions.
MINTi MINT-1889821.

Chemistry

BindingDBi O88871.
ChEMBLi CHEMBL2111474.
GuidetoPHARMACOLOGYi 241.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei O88871.

Proteomic databases

PaxDbi O88871.
PRIDEi O88871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 83633.
KEGGi rno:83633.
UCSCi RGD:619864. rat.

Organism-specific databases

CTDi 9568.
RGDi 619864. Gabbr2.

Phylogenomic databases

eggNOGi NOG273984.
HOGENOMi HOG000112688.
HOVERGENi HBG080355.
InParanoidi O88871.
KOi K04615.
PhylomeDBi O88871.
TreeFami TF313965.

Miscellaneous databases

NextBioi 616227.
PROi O88871.

Gene expression databases

Genevestigatori O88871.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view ]
PANTHERi PTHR10519. PTHR10519. 1 hit.
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view ]
PRINTSi PR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH GABBR1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain cortex and Cerebellum.
  3. "Distribution of the GABA(B) receptor subunit gb2 in rat CNS."
    Clark J.A., Mezey E., Lam A.S., Bonner T.I.
    Brain Res. 860:41-52(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain cortex.
  4. Borowsky B., Laz T., Gerald C.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  5. "Role of heteromer formation in GABAB receptor function."
    Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C.
    Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GABBR1, VARIANTS PRO-19 INS AND TYR-337.
    Tissue: Hippocampus.
  6. "The metabotropic GABAB receptor directly interacts with the activating transcription factor 4."
    Nehring R.B., Horikawa H.P.M., El Far O., Kneussel M., Brandstatter J.H., Stamm S., Wischmeyer E., Betz H., Karschin A.
    J. Biol. Chem. 275:35185-35191(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATF4.

Entry informationi

Entry nameiGABR2_RAT
AccessioniPrimary (citable) accession number: O88871
Secondary accession number(s): Q9JK36, Q9QWU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3