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Protein

Gamma-aminobutyric acid type B receptor subunit 2

Gene

Gabbr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

GO - Molecular functioni

  • G-protein coupled GABA receptor activity Source: RGD

GO - Biological processi

  • gamma-aminobutyric acid signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 2
Short name:
GABA-B receptor 2
Short name:
GABA-B-R2
Short name:
GABA-BR2
Short name:
GABABR2
Short name:
Gb2
Alternative name(s):
G-protein coupled receptor 51
Gene namesi
Name:Gabbr2
Synonyms:Gpr51
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619864. Gabbr2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini41 – 482ExtracellularSequence analysisAdd BLAST442
Transmembranei483 – 503Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini504 – 521CytoplasmicSequence analysisAdd BLAST18
Transmembranei522 – 542Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini543 – 550ExtracellularSequence analysis8
Transmembranei551 – 571Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini572 – 596CytoplasmicSequence analysisAdd BLAST25
Transmembranei597 – 617Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini618 – 653ExtracellularSequence analysisAdd BLAST36
Transmembranei654 – 674Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini675 – 690CytoplasmicSequence analysisAdd BLAST16
Transmembranei691 – 711Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini712 – 719ExtracellularSequence analysis8
Transmembranei720 – 740Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini741 – 940CytoplasmicSequence analysisAdd BLAST200

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111474.
GuidetoPHARMACOLOGYi241.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40Sequence analysisAdd BLAST40
ChainiPRO_000001295341 – 940Gamma-aminobutyric acid type B receptor subunit 2Add BLAST900

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi107 ↔ 134By similarity
Disulfide bondi236 ↔ 265By similarity
Disulfide bondi264 ↔ 301By similarity
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi388N-linked (GlcNAc...)Sequence analysis1
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Glycosylationi452N-linked (GlcNAc...)Sequence analysis1
Modified residuei775PhosphoserineBy similarity1
Modified residuei778PhosphoserineBy similarity1
Modified residuei818PhosphothreonineCombined sources1
Modified residuei883PhosphoserineCombined sources1
Modified residuei892PhosphoserineBy similarity1
Modified residuei912PhosphoserineBy similarity1
Modified residuei915PhosphoserineBy similarity1
Modified residuei919PhosphoserineBy similarity1
Modified residuei923PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88871.
PRIDEiO88871.

PTM databases

iPTMnetiO88871.
PhosphoSitePlusiO88871.
UniCarbKBiO88871.

Expressioni

Tissue specificityi

Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression is seen in cerebellum.2 Publications

Developmental stagei

Abundant in brain cortex and cerebellum throughout postnatal development whereas its expression in spinal cord gradually decreases.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cacna1dP277326EBI-7090239,EBI-8072674
Gabbr1Q9Z0U48EBI-7090239,EBI-7090268

Protein-protein interaction databases

BioGridi249797. 4 interactors.
IntActiO88871. 3 interactors.
MINTiMINT-1889821.
STRINGi10116.ENSRNOP00000011573.

Chemistry databases

BindingDBiO88871.

Structurei

3D structure databases

ProteinModelPortaliO88871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili780 – 818Sequence analysisAdd BLAST39

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiO88871.
KOiK04615.
PhylomeDBiO88871.
TreeFamiTF313965.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF40. PTHR10519:SF40. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPPSSGQP RPPPPPPPPA RLLLPLLLSL LLWLAPGAWG WTRGAPRPPP
60 70 80 90 100
SSPPLSIMGL MPLTKEVAKG SIGRGVLPAV ELAIEQIRNE SLLRPYFLDL
110 120 130 140 150
RLYDTECDNA KGLKAFYDAI KYGPNHLMVF GGVCPSVTSI IAESLQGWNL
160 170 180 190 200
VQLSFAATTP VLADKKKYPY FFRTVPSDNA VNPAILKLLK HFRWRRVGTL
210 220 230 240 250
TQDVQRFSEV RNDLTGVLYG EDIEISDTES FSNDPCTSVK KLKGNDVRII
260 270 280 290 300
LGQFDQNMAA KVFCCAFEES MFGSKYQWII PGWYEPAWWE QVHVEANSSR
310 320 330 340 350
CLRRSLLAAM EGYIGVDFEP LSSKQIKTIS GKTPQQFERE YNSKRSGVGP
360 370 380 390 400
SKFHGYAYDG IWVIAKTLQR AMETLHASSR HQRIQDFNYT DHTLGKIILN
410 420 430 440 450
AMNETNFFGV TGQVVFRNGE RMGTIKFTQF QDSREVKVGE YNAVADTLEI
460 470 480 490 500
INDTIRFQGS EPPKDKTIIL EQLRKISLPL YSILSALTIL GMIMASAFLF
510 520 530 540 550
FNIKNRNQKL IKMSSPYMNN LIILGGMLSY ASIFLFGLDG SFVSEKTFET
560 570 580 590 600
LCTVRTWILT VGYTTAFGAM FAKTWRVHAI FKNVKMKKKI IKDQKLLVIV
610 620 630 640 650
GGMLLIDLCI LICWQAVDPL RRTVERYSME PDPAGRDISI RPLLEHCENT
660 670 680 690 700
HMTIWLGIVY AYKGLLMLFG CFLAWETRNV SIPALNDSKY IGMSVYNVGI
710 720 730 740 750
MCIIGAAVSF LTRDQPNVQF CIVALVIIFC STITLCLVFV PKLITLRTNP
760 770 780 790 800
DAATQNRRFQ FTQNQKKEDS KTSTSVTSVN QASTSRLEGL QSENHRLRMK
810 820 830 840 850
ITELDKDLEE VTMQLQDTPE KTTYIKQNHY QELNDILSLG NFTESTDGGK
860 870 880 890 900
AILKNHLDQN PQLQWNTTEP SRTCKDPIED INSPEHIQRR LSLQLPILHH
910 920 930 940
AYLPSIGGVD ASCVSPCVSP TASPRHRHVP PSFRVMVSGL
Length:940
Mass (Da):105,751
Last modified:January 11, 2001 - v2
Checksum:i77BB42D833C7505D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti343S → T in CAA09592 (PubMed:9872317).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti19P → PP.1 Publication1
Natural varianti19P → R.1
Natural varianti337F → Y.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074482 mRNA. Translation: AAD03335.1.
AJ011318 Genomic DNA. Translation: CAA09592.1.
AF058795 mRNA. Translation: AAC63994.1.
AF109405 mRNA. Translation: AAD03338.1.
RefSeqiNP_113990.1. NM_031802.1.
UniGeneiRn.162814.

Genome annotation databases

GeneIDi83633.
KEGGirno:83633.
UCSCiRGD:619864. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074482 mRNA. Translation: AAD03335.1.
AJ011318 Genomic DNA. Translation: CAA09592.1.
AF058795 mRNA. Translation: AAC63994.1.
AF109405 mRNA. Translation: AAD03338.1.
RefSeqiNP_113990.1. NM_031802.1.
UniGeneiRn.162814.

3D structure databases

ProteinModelPortaliO88871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249797. 4 interactors.
IntActiO88871. 3 interactors.
MINTiMINT-1889821.
STRINGi10116.ENSRNOP00000011573.

Chemistry databases

BindingDBiO88871.
ChEMBLiCHEMBL2111474.
GuidetoPHARMACOLOGYi241.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiO88871.
PhosphoSitePlusiO88871.
UniCarbKBiO88871.

Proteomic databases

PaxDbiO88871.
PRIDEiO88871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83633.
KEGGirno:83633.
UCSCiRGD:619864. rat.

Organism-specific databases

CTDi9568.
RGDi619864. Gabbr2.

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
HOGENOMiHOG000112688.
HOVERGENiHBG080355.
InParanoidiO88871.
KOiK04615.
PhylomeDBiO88871.
TreeFamiTF313965.

Miscellaneous databases

PROiO88871.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR002457. GPCR_3_GABA_rcpt_B2.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF40. PTHR10519:SF40. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR01178. GABAB2RECPTR.
PR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABR2_RAT
AccessioniPrimary (citable) accession number: O88871
Secondary accession number(s): Q9JK36, Q9QWU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.