ID   BEST1_MOUSE             Reviewed;         551 AA.
AC   O88870; B2RSY6; Q6H1V0;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 3.
DT   27-MAR-2024, entry version 154.
DE   RecName: Full=Bestrophin-1;
DE   AltName: Full=Vitelliform macular dystrophy protein 2 homolog;
GN   Name=Best1; Synonyms=Bmd1, Vmd2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=15218265; DOI=10.1159/000078016;
RA   Kraemer F., Stoehr H., Weber B.H.F.;
RT   "Cloning and characterization of the murine Vmd2 RFP-TM gene family.";
RL   Cytogenet. Genome Res. 105:107-114(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 178-258.
RX   PubMed=9662395; DOI=10.1038/915;
RA   Petrukhin K., Koisti M.J., Bakall B., Li W., Xie G., Marknell T.,
RA   Sandgren O., Forsman K., Holmgren G., Andreasson S., Vujic M.,
RA   Bergen A.A.B., McGarty-Dugan V., Figueroa D., Austin C.P., Metzker M.L.,
RA   Caskey C.T., Wadelius C.;
RT   "Identification of the gene responsible for Best macular dystrophy.";
RL   Nat. Genet. 19:241-247(1998).
CC   -!- FUNCTION: Forms calcium-sensitive chloride channels. Permeable to
CC       bicarbonate (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Tetramer or pentamers. May interact with PPP2CB and PPP2R1B
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC       Basolateral cell membrane {ECO:0000250}.
CC   -!- PTM: Phosphorylated by PP2A. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the anion channel-forming bestrophin (TC 1.A.46)
CC       family. Calcium-sensitive chloride channel subfamily. {ECO:0000305}.
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DR   EMBL; AY450427; AAS09922.1; -; mRNA.
DR   EMBL; BC139060; AAI39061.1; -; mRNA.
DR   EMBL; BC139061; AAI39062.1; -; mRNA.
DR   EMBL; AF057171; AAC64345.1; -; mRNA.
DR   CCDS; CCDS29568.1; -.
DR   RefSeq; NP_036043.2; NM_011913.2.
DR   AlphaFoldDB; O88870; -.
DR   SMR; O88870; -.
DR   STRING; 10090.ENSMUSP00000113053; -.
DR   iPTMnet; O88870; -.
DR   PhosphoSitePlus; O88870; -.
DR   EPD; O88870; -.
DR   PaxDb; 10090-ENSMUSP00000113053; -.
DR   ProteomicsDB; 273555; -.
DR   Antibodypedia; 28370; 343 antibodies from 29 providers.
DR   DNASU; 24115; -.
DR   Ensembl; ENSMUST00000117346.2; ENSMUSP00000113053.2; ENSMUSG00000037418.7.
DR   GeneID; 24115; -.
DR   KEGG; mmu:24115; -.
DR   UCSC; uc008gou.2; mouse.
DR   AGR; MGI:1346332; -.
DR   CTD; 7439; -.
DR   MGI; MGI:1346332; Best1.
DR   VEuPathDB; HostDB:ENSMUSG00000037418; -.
DR   eggNOG; KOG3547; Eukaryota.
DR   GeneTree; ENSGT00940000158650; -.
DR   HOGENOM; CLU_018069_5_0_1; -.
DR   InParanoid; O88870; -.
DR   OMA; NLAMQAW; -.
DR   OrthoDB; 2871698at2759; -.
DR   PhylomeDB; O88870; -.
DR   TreeFam; TF315803; -.
DR   Reactome; R-MMU-2672351; Stimuli-sensing channels.
DR   BioGRID-ORCS; 24115; 1 hit in 77 CRISPR screens.
DR   PRO; PR:O88870; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; O88870; Protein.
DR   Bgee; ENSMUSG00000037418; Expressed in seminiferous tubule of testis and 89 other cell types or tissues.
DR   ExpressionAtlas; O88870; baseline and differential.
DR   GO; GO:0009925; C:basal plasma membrane; ISO:MGI.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR   GO; GO:0034707; C:chloride channel complex; ISO:MGI.
DR   GO; GO:0005254; F:chloride channel activity; ISO:MGI.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0006821; P:chloride transport; ISO:MGI.
DR   GO; GO:0050908; P:detection of light stimulus involved in visual perception; IMP:MGI.
DR   GO; GO:0051924; P:regulation of calcium ion transport; IMP:MGI.
DR   GO; GO:0030321; P:transepithelial chloride transport; ISO:MGI.
DR   InterPro; IPR000615; Bestrophin.
DR   InterPro; IPR021134; Bestrophin-like.
DR   PANTHER; PTHR10736; BESTROPHIN; 1.
DR   PANTHER; PTHR10736:SF4; BESTROPHIN-1; 1.
DR   Pfam; PF01062; Bestrophin; 1.
DR   Genevisible; O88870; MM.
PE   2: Evidence at transcript level;
KW   Calcium; Cell membrane; Chloride; Chloride channel; Ion channel;
KW   Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..551
FT                   /note="Bestrophin-1"
FT                   /id="PRO_0000143116"
FT   TOPO_DOM        1..25
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        47..70
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..178
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..228
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   INTRAMEM        229..249
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        250..270
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        292..551
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          459..489
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        464..486
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        255
FT                   /note="R -> K (in Ref. 1; AAS09922 and 3; AAC64345)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   551 AA;  63684 MW;  0898D6DE08FEEFB9 CRC64;
     MTITYTNKVA NARLGSFSSL LLCWRGSIYK LLYGEFLVFI FLYYSIRGLY RMVLSSDQQL
     LFEKLALYCD SYIQLIPISF VLGFYVTLVV SRWWSQYENL PWPDRLMIQV SSFVEGKDEE
     GRLLRRTLIR YAILGQVLIL RSISTSVYKR FPTLHHLVLA GFMTHGEHKQ LQKLGLPHNT
     FWVPWVWFAN LSMKAYLGGR IRDTVLLQSL MNEVCTLRTQ CGQLYAYDWI SIPLVYTQVV
     TVAVYSFFLA CLIGRQFLNP NKDYPGHEMD LVVPVFTILQ FLFYMGWLKV AEQLINPFGE
     DDDDFETNWI IDRNLQVSLL SVDGMHQNLP PMERDMYWNE AAPQPPYTAA SARSRRHSFM
     GSTFNISLKK EDLELWSKEE ADTDKKESGY SSTIGCFLGL QPKNYHLPLK DLKTKLLCSK
     NPLLEGQCKD ANQKNQKDVW KFKGLDFLKC VPRFKRRGSH CGPQAPSSHP TEQSAPSSSD
     TGDGPSTDYQ EICHMKKKTV EFNLNIPESP TEHLQQRRLD QMSTNIQALM KEHAESYPYR
     DEAGTKPVLY E
//