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Protein

Bestrophin-1

Gene

Best1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms calcium-sensitive chloride channels. Permeable to bicarbonate (By similarity).By similarity

GO - Molecular functioni

  1. chloride channel activity Source: MGI

GO - Biological processi

  1. chloride transmembrane transport Source: GOC
  2. chloride transport Source: MGI
  3. detection of light stimulus involved in visual perception Source: MGI
  4. regulation of calcium ion transport Source: MGI
  5. transepithelial chloride transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride

Enzyme and pathway databases

ReactomeiREACT_297274. Orphan transporters.
REACT_346473. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Bestrophin-1
Alternative name(s):
Vitelliform macular dystrophy protein 2 homolog
Gene namesi
Name:Best1
Synonyms:Bmd1, Vmd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1346332. Best1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei26 – 4621HelicalSequence AnalysisAdd
BLAST
Topological domaini47 – 7024ExtracellularSequence AnalysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Topological domaini92 – 17887CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence AnalysisAdd
BLAST
Topological domaini200 – 22829ExtracellularSequence AnalysisAdd
BLAST
Intramembranei229 – 24921Sequence AnalysisAdd
BLAST
Topological domaini250 – 27021ExtracellularSequence AnalysisAdd
BLAST
Transmembranei271 – 29121HelicalSequence AnalysisAdd
BLAST
Topological domaini292 – 551260CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. chloride channel complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Bestrophin-1PRO_0000143116Add
BLAST

Post-translational modificationi

Phosphorylated by PP2A.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO88870.

PTM databases

PhosphoSiteiO88870.

Expressioni

Gene expression databases

BgeeiO88870.
CleanExiMM_BEST1.
GenevestigatoriO88870.

Interactioni

Subunit structurei

Tetramer or pentamers. May interact with PPP2CB and PPP2R1B (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO88870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bestrophin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG315004.
GeneTreeiENSGT00390000002997.
HOGENOMiHOG000115678.
HOVERGENiHBG044928.
InParanoidiO88870.
KOiK13878.
OMAiMTITYTN.
OrthoDBiEOG7N0C43.
TreeFamiTF315803.

Family and domain databases

InterProiIPR000615. Bestrophin.
IPR021134. Bestrophin/UPF0187.
[Graphical view]
PANTHERiPTHR10736. PTHR10736. 1 hit.
PfamiPF01062. Bestrophin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITYTNKVA NARLGSFSSL LLCWRGSIYK LLYGEFLVFI FLYYSIRGLY
60 70 80 90 100
RMVLSSDQQL LFEKLALYCD SYIQLIPISF VLGFYVTLVV SRWWSQYENL
110 120 130 140 150
PWPDRLMIQV SSFVEGKDEE GRLLRRTLIR YAILGQVLIL RSISTSVYKR
160 170 180 190 200
FPTLHHLVLA GFMTHGEHKQ LQKLGLPHNT FWVPWVWFAN LSMKAYLGGR
210 220 230 240 250
IRDTVLLQSL MNEVCTLRTQ CGQLYAYDWI SIPLVYTQVV TVAVYSFFLA
260 270 280 290 300
CLIGRQFLNP NKDYPGHEMD LVVPVFTILQ FLFYMGWLKV AEQLINPFGE
310 320 330 340 350
DDDDFETNWI IDRNLQVSLL SVDGMHQNLP PMERDMYWNE AAPQPPYTAA
360 370 380 390 400
SARSRRHSFM GSTFNISLKK EDLELWSKEE ADTDKKESGY SSTIGCFLGL
410 420 430 440 450
QPKNYHLPLK DLKTKLLCSK NPLLEGQCKD ANQKNQKDVW KFKGLDFLKC
460 470 480 490 500
VPRFKRRGSH CGPQAPSSHP TEQSAPSSSD TGDGPSTDYQ EICHMKKKTV
510 520 530 540 550
EFNLNIPESP TEHLQQRRLD QMSTNIQALM KEHAESYPYR DEAGTKPVLY

E
Length:551
Mass (Da):63,684
Last modified:July 26, 2011 - v3
Checksum:i0898D6DE08FEEFB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551R → K in AAS09922 (PubMed:15218265).Curated
Sequence conflicti255 – 2551R → K in AAC64345 (PubMed:9662395).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY450427 mRNA. Translation: AAS09922.1.
BC139060 mRNA. Translation: AAI39061.1.
BC139061 mRNA. Translation: AAI39062.1.
AF057171 mRNA. Translation: AAC64345.1.
CCDSiCCDS29568.1.
RefSeqiNP_036043.2. NM_011913.2.
UniGeneiMm.31577.

Genome annotation databases

EnsembliENSMUST00000117346; ENSMUSP00000113053; ENSMUSG00000037418.
GeneIDi24115.
KEGGimmu:24115.
UCSCiuc008gou.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY450427 mRNA. Translation: AAS09922.1.
BC139060 mRNA. Translation: AAI39061.1.
BC139061 mRNA. Translation: AAI39062.1.
AF057171 mRNA. Translation: AAC64345.1.
CCDSiCCDS29568.1.
RefSeqiNP_036043.2. NM_011913.2.
UniGeneiMm.31577.

3D structure databases

ProteinModelPortaliO88870.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO88870.

Proteomic databases

PRIDEiO88870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117346; ENSMUSP00000113053; ENSMUSG00000037418.
GeneIDi24115.
KEGGimmu:24115.
UCSCiuc008gou.2. mouse.

Organism-specific databases

CTDi7439.
MGIiMGI:1346332. Best1.

Phylogenomic databases

eggNOGiNOG315004.
GeneTreeiENSGT00390000002997.
HOGENOMiHOG000115678.
HOVERGENiHBG044928.
InParanoidiO88870.
KOiK13878.
OMAiMTITYTN.
OrthoDBiEOG7N0C43.
TreeFamiTF315803.

Enzyme and pathway databases

ReactomeiREACT_297274. Orphan transporters.
REACT_346473. Stimuli-sensing channels.

Miscellaneous databases

NextBioi304147.
PROiO88870.
SOURCEiSearch...

Gene expression databases

BgeeiO88870.
CleanExiMM_BEST1.
GenevestigatoriO88870.

Family and domain databases

InterProiIPR000615. Bestrophin.
IPR021134. Bestrophin/UPF0187.
[Graphical view]
PANTHERiPTHR10736. PTHR10736. 1 hit.
PfamiPF01062. Bestrophin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the murine Vmd2 RFP-TM gene family."
    Kraemer F., Stoehr H., Weber B.H.F.
    Cytogenet. Genome Res. 105:107-114(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-258.

Entry informationi

Entry nameiBEST1_MOUSE
AccessioniPrimary (citable) accession number: O88870
Secondary accession number(s): B2RSY6, Q6H1V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1999
Last sequence update: July 26, 2011
Last modified: March 31, 2015
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.