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O88870 (BEST1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bestrophin-1
Alternative name(s):
Vitelliform macular dystrophy protein 2 homolog
Gene names
Name:Best1
Synonyms:Bmd1, Vmd2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Forms calcium-sensitive chloride channels. Permeable to bicarbonate By similarity.

Subunit structure

Tetramer or pentamers. May interact with PPP2CB and PPP2R1B By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane By similarity.

Post-translational modification

Phosphorylated by PP2A By similarity.

Sequence similarities

Belongs to the bestrophin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Bestrophin-1
PRO_0000143116

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621Helical; Potential
Topological domain47 – 7024Extracellular Potential
Transmembrane71 – 9121Helical; Potential
Topological domain92 – 17887Cytoplasmic Potential
Transmembrane179 – 19921Helical; Potential
Topological domain200 – 22829Extracellular Potential
Intramembrane229 – 24921 Potential
Topological domain250 – 27021Extracellular Potential
Transmembrane271 – 29121Helical; Potential
Topological domain292 – 551260Cytoplasmic Potential

Experimental info

Sequence conflict2551R → K in AAS09922. Ref.1
Sequence conflict2551R → K in AAC64345. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O88870 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 0898D6DE08FEEFB9

FASTA55163,684
        10         20         30         40         50         60 
MTITYTNKVA NARLGSFSSL LLCWRGSIYK LLYGEFLVFI FLYYSIRGLY RMVLSSDQQL 

        70         80         90        100        110        120 
LFEKLALYCD SYIQLIPISF VLGFYVTLVV SRWWSQYENL PWPDRLMIQV SSFVEGKDEE 

       130        140        150        160        170        180 
GRLLRRTLIR YAILGQVLIL RSISTSVYKR FPTLHHLVLA GFMTHGEHKQ LQKLGLPHNT 

       190        200        210        220        230        240 
FWVPWVWFAN LSMKAYLGGR IRDTVLLQSL MNEVCTLRTQ CGQLYAYDWI SIPLVYTQVV 

       250        260        270        280        290        300 
TVAVYSFFLA CLIGRQFLNP NKDYPGHEMD LVVPVFTILQ FLFYMGWLKV AEQLINPFGE 

       310        320        330        340        350        360 
DDDDFETNWI IDRNLQVSLL SVDGMHQNLP PMERDMYWNE AAPQPPYTAA SARSRRHSFM 

       370        380        390        400        410        420 
GSTFNISLKK EDLELWSKEE ADTDKKESGY SSTIGCFLGL QPKNYHLPLK DLKTKLLCSK 

       430        440        450        460        470        480 
NPLLEGQCKD ANQKNQKDVW KFKGLDFLKC VPRFKRRGSH CGPQAPSSHP TEQSAPSSSD 

       490        500        510        520        530        540 
TGDGPSTDYQ EICHMKKKTV EFNLNIPESP TEHLQQRRLD QMSTNIQALM KEHAESYPYR 

       550 
DEAGTKPVLY E 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the murine Vmd2 RFP-TM gene family."
Kraemer F., Stoehr H., Weber B.H.F.
Cytogenet. Genome Res. 105:107-114(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[3]"Identification of the gene responsible for Best macular dystrophy."
Petrukhin K., Koisti M.J., Bakall B., Li W., Xie G., Marknell T., Sandgren O., Forsman K., Holmgren G., Andreasson S., Vujic M., Bergen A.A.B., McGarty-Dugan V., Figueroa D., Austin C.P., Metzker M.L., Caskey C.T., Wadelius C.
Nat. Genet. 19:241-247(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-258.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY450427 mRNA. Translation: AAS09922.1.
BC139060 mRNA. Translation: AAI39061.1.
BC139061 mRNA. Translation: AAI39062.1.
AF057171 mRNA. Translation: AAC64345.1.
RefSeqNP_036043.2. NM_011913.2.
UniGeneMm.31577.

3D structure databases

ProteinModelPortalO88870.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO88870.

Proteomic databases

PRIDEO88870.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000117346; ENSMUSP00000113053; ENSMUSG00000037418.
GeneID24115.
KEGGmmu:24115.
UCSCuc008gou.2. mouse.

Organism-specific databases

CTD7439.
MGIMGI:1346332. Best1.

Phylogenomic databases

eggNOGNOG315004.
GeneTreeENSGT00390000002997.
HOGENOMHOG000115678.
HOVERGENHBG044928.
InParanoidB2RSY6.
KOK13878.
OMARSGYHSA.
OrthoDBEOG7N0C43.
TreeFamTF315803.

Gene expression databases

BgeeO88870.
CleanExMM_BEST1.
GenevestigatorO88870.

Family and domain databases

InterProIPR000615. Bestrophin.
IPR021134. Bestrophin/UPF0187.
[Graphical view]
PANTHERPTHR10736. PTHR10736. 1 hit.
PfamPF01062. Bestrophin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304147.
PROO88870.
SOURCESearch...

Entry information

Entry nameBEST1_MOUSE
AccessionPrimary (citable) accession number: O88870
Secondary accession number(s): B2RSY6, Q6H1V0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot