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O88870

- BEST1_MOUSE

UniProt

O88870 - BEST1_MOUSE

Protein

Bestrophin-1

Gene

Best1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Forms calcium-sensitive chloride channels. Permeable to bicarbonate By similarity.By similarity

    GO - Molecular functioni

    1. chloride channel activity Source: UniProtKB-KW

    GO - Biological processi

    1. detection of light stimulus involved in visual perception Source: MGI
    2. regulation of calcium ion transport Source: MGI
    3. transepithelial chloride transport Source: HGNC

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calcium, Chloride

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bestrophin-1
    Alternative name(s):
    Vitelliform macular dystrophy protein 2 homolog
    Gene namesi
    Name:Best1
    Synonyms:Bmd1, Vmd2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1346332. Best1.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. chloride channel complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Bestrophin-1PRO_0000143116Add
    BLAST

    Post-translational modificationi

    Phosphorylated by PP2A.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO88870.

    PTM databases

    PhosphoSiteiO88870.

    Expressioni

    Gene expression databases

    BgeeiO88870.
    CleanExiMM_BEST1.
    GenevestigatoriO88870.

    Interactioni

    Subunit structurei

    Tetramer or pentamers. May interact with PPP2CB and PPP2R1B By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO88870.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini47 – 7024ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini92 – 17887CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini200 – 22829ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini250 – 27021ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini292 – 551260CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei229 – 24921Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei179 – 19921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei271 – 29121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bestrophin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG315004.
    GeneTreeiENSGT00390000002997.
    HOGENOMiHOG000115678.
    HOVERGENiHBG044928.
    InParanoidiB2RSY6.
    KOiK13878.
    OMAiRSGYHSA.
    OrthoDBiEOG7N0C43.
    TreeFamiTF315803.

    Family and domain databases

    InterProiIPR000615. Bestrophin.
    IPR021134. Bestrophin/UPF0187.
    [Graphical view]
    PANTHERiPTHR10736. PTHR10736. 1 hit.
    PfamiPF01062. Bestrophin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTITYTNKVA NARLGSFSSL LLCWRGSIYK LLYGEFLVFI FLYYSIRGLY    50
    RMVLSSDQQL LFEKLALYCD SYIQLIPISF VLGFYVTLVV SRWWSQYENL 100
    PWPDRLMIQV SSFVEGKDEE GRLLRRTLIR YAILGQVLIL RSISTSVYKR 150
    FPTLHHLVLA GFMTHGEHKQ LQKLGLPHNT FWVPWVWFAN LSMKAYLGGR 200
    IRDTVLLQSL MNEVCTLRTQ CGQLYAYDWI SIPLVYTQVV TVAVYSFFLA 250
    CLIGRQFLNP NKDYPGHEMD LVVPVFTILQ FLFYMGWLKV AEQLINPFGE 300
    DDDDFETNWI IDRNLQVSLL SVDGMHQNLP PMERDMYWNE AAPQPPYTAA 350
    SARSRRHSFM GSTFNISLKK EDLELWSKEE ADTDKKESGY SSTIGCFLGL 400
    QPKNYHLPLK DLKTKLLCSK NPLLEGQCKD ANQKNQKDVW KFKGLDFLKC 450
    VPRFKRRGSH CGPQAPSSHP TEQSAPSSSD TGDGPSTDYQ EICHMKKKTV 500
    EFNLNIPESP TEHLQQRRLD QMSTNIQALM KEHAESYPYR DEAGTKPVLY 550
    E 551
    Length:551
    Mass (Da):63,684
    Last modified:July 27, 2011 - v3
    Checksum:i0898D6DE08FEEFB9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti255 – 2551R → K in AAS09922. (PubMed:15218265)Curated
    Sequence conflicti255 – 2551R → K in AAC64345. (PubMed:9662395)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY450427 mRNA. Translation: AAS09922.1.
    BC139060 mRNA. Translation: AAI39061.1.
    BC139061 mRNA. Translation: AAI39062.1.
    AF057171 mRNA. Translation: AAC64345.1.
    CCDSiCCDS29568.1.
    RefSeqiNP_036043.2. NM_011913.2.
    UniGeneiMm.31577.

    Genome annotation databases

    EnsembliENSMUST00000117346; ENSMUSP00000113053; ENSMUSG00000037418.
    GeneIDi24115.
    KEGGimmu:24115.
    UCSCiuc008gou.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY450427 mRNA. Translation: AAS09922.1 .
    BC139060 mRNA. Translation: AAI39061.1 .
    BC139061 mRNA. Translation: AAI39062.1 .
    AF057171 mRNA. Translation: AAC64345.1 .
    CCDSi CCDS29568.1.
    RefSeqi NP_036043.2. NM_011913.2.
    UniGenei Mm.31577.

    3D structure databases

    ProteinModelPortali O88870.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O88870.

    Proteomic databases

    PRIDEi O88870.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000117346 ; ENSMUSP00000113053 ; ENSMUSG00000037418 .
    GeneIDi 24115.
    KEGGi mmu:24115.
    UCSCi uc008gou.2. mouse.

    Organism-specific databases

    CTDi 7439.
    MGIi MGI:1346332. Best1.

    Phylogenomic databases

    eggNOGi NOG315004.
    GeneTreei ENSGT00390000002997.
    HOGENOMi HOG000115678.
    HOVERGENi HBG044928.
    InParanoidi B2RSY6.
    KOi K13878.
    OMAi RSGYHSA.
    OrthoDBi EOG7N0C43.
    TreeFami TF315803.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 304147.
    PROi O88870.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88870.
    CleanExi MM_BEST1.
    Genevestigatori O88870.

    Family and domain databases

    InterProi IPR000615. Bestrophin.
    IPR021134. Bestrophin/UPF0187.
    [Graphical view ]
    PANTHERi PTHR10736. PTHR10736. 1 hit.
    Pfami PF01062. Bestrophin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the murine Vmd2 RFP-TM gene family."
      Kraemer F., Stoehr H., Weber B.H.F.
      Cytogenet. Genome Res. 105:107-114(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-258.

    Entry informationi

    Entry nameiBEST1_MOUSE
    AccessioniPrimary (citable) accession number: O88870
    Secondary accession number(s): B2RSY6, Q6H1V0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3