O88869 (RASF9_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras association domain-containing protein 9

Alternative name(s):
PAM COOH-terminal interactor protein 1
Short name=P-CIP1
Peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor

Gene names

Name:	Rassf9
Synonyms:	Pamci, Pcip1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in regulating vesicuar trafficking in cells. Ref.1
Subunit structure	Interacts with PAM. Ref.1
Subcellular location	Endosome. Note: Accumulates on perinuclear endosomes. Ref.1
Tissue specificity	Testis, kidney, skeletal muscle, liver, lung, brain, heart, pituitary gland, adrenal gland and ovary. Ref.1
Sequence similarities	Contains 1 Ras-associating domain.

Ontologies

Keywords
Cellular component	Endosome
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endosome transport Non-traceable author statement Ref.1. Source: UniProtKB protein targeting Non-traceable author statement Ref.1. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB endosome Inferred from direct assay Ref.1. Source: UniProtKB insoluble fraction Inferred from direct assay Ref.1. Source: RGD trans-Golgi network transport vesicle membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB transporter activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pam	P14925	3	EBI-1395057,EBI-1395008

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Ras association domain-containing protein 9		PRO_0000299454
Regions
	Domain	25 – 119	95	Ras-associating
	Coiled coil	195 – 291	97	Potential

Sequences

	Sequence		Length	Mass (Da)
	O88869 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 582AF0C52176E9B1	FASTA	435	49,545
	10 20 30 40 50 60 MAPFGRNLLK TRHKNRSPTK DMDPEEKEIV VWVCQEEKIV CGLTKRTTSI DVIQALLEEH 70 80 90 100 110 120 EATFGEKRFL LGKASDYCIV EKWRGSERAL PPLTRILKLW KAWGDEQPNM QFVLVKTDAF 130 140 150 160 170 180 LPVPLWRTAE TKLVQNNEKP WELSPANYMK TLPPDKQKRI VRKTFRKLAK IRQDTGSHDR 190 200 210 220 230 240 DNMECLVHLI ISQDHTIHQQ VQRMKELDME IEKCEAKIHL DRIGNDGADY VQEAYLMPRS 250 260 270 280 290 300 SEEEQKLDFQ SEDNQTLEDL NDGEGVSQLE EQLQYYRALI DKLSAEIERE VKGAGTDGSE 310 320 330 340 350 360 DMEGAAACEL ENSDLESVKC DLEKSMKAGL KIHSHLSGIQ REIKYSDSLL QMKAREYELL 370 380 390 400 410 420 AKEFSSLHIS SKDGCQGKEN RGKEAEASSS NGEIPPLTQR VFNTYTNDTD SDTGISSNHS 430 QDSETTLGDV LLLAT

References

[1]

"P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes." Chen L., Johnson R.C., Milgram S.L. J. Biol. Chem. 273:33524-33532(1998) [PubMed: 9837933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PAM, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF056208 mRNA. Translation: AAD03249.1.
IPI	IPI00213427.
RefSeq	NP_075248.1. NM_022959.1.
UniGene	Rn.30013.
3D structure databases
HSSP	HSSP built from PDB template 2CS4 based on UniProtKB Q8NHQ8.
ProteinModelPortal	O88869.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88869. 1 interaction.
STRING	O88869.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	65053.
KEGG	rno:65053.
UCSC	NM_022959. rat.
Organism-specific databases
CTD	9182.
RGD	621329. Rassf9.
Phylogenomic databases
eggNOG	maNOG07529.
GeneTree	ENSGT00510000047164.
HOVERGEN	HBG053178.
InParanoid	O88869.
OrthoDB	EOG4T4CVG.
Gene expression databases
ArrayExpress	O88869.
Genevestigator	O88869.
Family and domain databases
InterPro	IPR000159. Ras-assoc. [Graphical view]
SMART	SM00314. RA. 1 hit. [Graphical view]
PROSITE	PS50200. RA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613907.

Entry information

Entry name

RASF9_RAT

Accession

Primary (citable) accession number: O88869

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 1, 1998
Last modified:	September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program