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Protein

Homeodomain-interacting protein kinase 3

Gene

Hipk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226ATPPROSITE-ProRule annotation1
Active sitei322Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi203 – 211ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-interacting protein kinase 3 (EC:2.7.11.1)
Alternative name(s):
Androgen receptor-interacting nuclear protein kinase
Short name:
ANPK
Gene namesi
Name:Hipk3
Synonyms:Fist
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619884. Hipk3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi226K → R: No enzymatic activity. 1 Publication1
Mutagenesisi357S → A: Reduces enzymatic activity but no effect on autophosphorylation; when associated with A-359. 1 Publication1
Mutagenesisi359Y → A: Reduces enzymatic activity; but no effect on autophosphorylation; when associated with A-357. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860001 – 1191Homeodomain-interacting protein kinase 3Add BLAST1191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei359PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated. Autophosphorylation is not required for catalytic activity.
May be sumoylated.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88850.
PRIDEiO88850.

PTM databases

iPTMnetiO88850.
PhosphoSitePlusiO88850.

Interactioni

Subunit structurei

Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with UBL1/SUMO-1 (By similarity). Interacts with and stabilizes ligand-bound androgen receptor (AR).By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-5932N.
STRINGi10116.ENSRNOP00000015775.

Structurei

3D structure databases

ProteinModelPortaliO88850.
SMRiO88850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 525Protein kinasePROSITE-ProRule annotationAdd BLAST329

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni766 – 920Interaction with AR1 PublicationAdd BLAST155
Regioni774 – 867Interaction with FASBy similarityAdd BLAST94
Regioni846 – 856Interaction with UBL1By similarityAdd BLAST11

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi886 – 938Ser-richAdd BLAST53

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOVERGENiHBG051908.
InParanoidiO88850.
KOiK08826.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

O88850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQVLVYPP YVYQTQSSAF CSVKKLKVEP SGCVFQERAC PQIHVNGRHF
60 70 80 90 100
GNPLPSTKGS AFQTKIPFSK PRGHSFSLQA GAIVVKDTAG ATKVIAAQAQ
110 120 130 140 150
QAGVEAPRAV VWRNRLHFLG GPQRCGLKRK SEELDNHSGA MQIVDELSIL
160 170 180 190 200
PAMLQTNMGN PVTVVTATTG SKQNCTSGEG DYQLVQHEVL CSVKNTYEVL
210 220 230 240 250
DFLGRGTFGQ VVKCWKRGTN EIVAIKILKN HPSYARQGQI EVSILARLST
260 270 280 290 300
ENADEYNFVR AYECFQHRNH TCLVFEMLEQ NLYDFLKQNK FSPLPLKVIR
310 320 330 340 350
PVLQQVATAL KKLKSLGLIH ADLKPENIML VDPVRQPYRV KVIDFGSASH
360 370 380 390 400
VSKTVCSTYL QSRYYRAPEI ILGLPFCEAI DMWSLGCVIA ELFLGWPLYP
410 420 430 440 450
GALEYDQIRY ISQTQGLPGE QLLNVGTKST RFFCRETDMS HSGWRLKTLE
460 470 480 490 500
EHEAETGMKS KEARKYIFNS LDDIVHVNTV MDLEGSDLLA EKADRREFVS
510 520 530 540 550
LLKKMLLIDA DLRITPIETL NHPFVSMKHL LDFPHSSHVK SCFHIMDICK
560 570 580 590 600
SPSSCETNNH SKMSLLRPVA SNGTAALAAN FTKVGTLRSQ ALTTSAHSVV
610 620 630 640 650
HHGIPLQAGT AQFGCGDAFH QTLIICPPAI QGIPAAHGKP TSYSIRVDNT
660 670 680 690 700
VPLVTQAPAV QPLQIRPGVL SQTWSGRTQQ MLIPAWQQVT PMAPAAATLT
710 720 730 740 750
SEGMAGSQRL GDWGKMIPHS NHYNSVMPPP LLTNQITLSA PQPISVGIAH
760 770 780 790 800
VVWPQPATTK KNKLCQNRSN SLQNTNVPHS AFISPKIISG KEVEEVSCVE
810 820 830 840 850
TQDNHTSEGE ARTCHEASVR QDSSVSDKQR QTIIIADSPS PAVSVITISS
860 870 880 890 900
DTDDEETSPR PSLRECKGSL DCEACQSTLN IDRMCSLSSP DSTLSTSSSG
910 920 930 940 950
QSSPSPCKRP NSMSDDEQES GCETVDGSPT SDSSGHDSPF AESSFVEDTP
960 970 980 990 1000
QNPELGTCAG TEAKPALSTA VEPPVGTERG LNVDAHMANT DSTCQPLTKG
1010 1020 1030 1040 1050
QPAPGKLNQP SASAARQQKP TSAFQQQHLN LSQVQHFGTG HQEWNGNFGH
1060 1070 1080 1090 1100
RRQQAYIPTS VTSNPFTLSH GSPNHTAVHA HLAGSAHLGG QPTLLPYPPS
1110 1120 1130 1140 1150
TALSSAAPVA HLLASPCTSR PMLQHPTYNI SHPSGIVHQV PVGINPRLLP
1160 1170 1180 1190
SPTIHQTQYK PIFPPHSYIA ASPAYTGFPL SPTKLSQYPY M
Length:1,191
Mass (Da):129,744
Last modified:November 1, 1998 - v1
Checksum:i0C2D05ED947A53ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036959 mRNA. Translation: AAC35249.1.
PIRiT14154.
RefSeqiNP_113975.1. NM_031787.1.
UniGeneiRn.8099.

Genome annotation databases

GeneIDi83617.
KEGGirno:83617.
UCSCiRGD:619884. rat.

Similar proteinsi

Entry informationi

Entry nameiHIPK3_RAT
AccessioniPrimary (citable) accession number: O88850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families