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Protein

Homeodomain-interacting protein kinase 3

Gene

Hipk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261ATPPROSITE-ProRule annotation
Active sitei322 – 3221Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi203 – 2119ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-interacting protein kinase 3 (EC:2.7.11.1)
Alternative name(s):
Androgen receptor-interacting nuclear protein kinase
Short name:
ANPK
Gene namesi
Name:Hipk3
Synonyms:Fist
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619884. Hipk3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi226 – 2261K → R: No enzymatic activity. 1 Publication
Mutagenesisi357 – 3571S → A: Reduces enzymatic activity but no effect on autophosphorylation; when associated with A-359. 1 Publication
Mutagenesisi359 – 3591Y → A: Reduces enzymatic activity; but no effect on autophosphorylation; when associated with A-357. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11911191Homeodomain-interacting protein kinase 3PRO_0000086000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei359 – 3591PhosphotyrosineBy similarity

Post-translational modificationi

Autophosphorylated. Autophosphorylation is not required for catalytic activity.
May be sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88850.
PRIDEiO88850.

PTM databases

PhosphoSiteiO88850.

Interactioni

Subunit structurei

Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with UBL1/SUMO-1 (By similarity). Interacts with and stabilizes ligand-bound androgen receptor (AR).By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-5932N.
STRINGi10116.ENSRNOP00000015775.

Structurei

3D structure databases

ProteinModelPortaliO88850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini197 – 525329Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni766 – 920155Interaction with ARAdd
BLAST
Regioni774 – 86794Interaction with FASBy similarityAdd
BLAST
Regioni846 – 85611Interaction with UBL1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi886 – 93853Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOVERGENiHBG051908.
InParanoidiO88850.
KOiK08826.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQVLVYPP YVYQTQSSAF CSVKKLKVEP SGCVFQERAC PQIHVNGRHF
60 70 80 90 100
GNPLPSTKGS AFQTKIPFSK PRGHSFSLQA GAIVVKDTAG ATKVIAAQAQ
110 120 130 140 150
QAGVEAPRAV VWRNRLHFLG GPQRCGLKRK SEELDNHSGA MQIVDELSIL
160 170 180 190 200
PAMLQTNMGN PVTVVTATTG SKQNCTSGEG DYQLVQHEVL CSVKNTYEVL
210 220 230 240 250
DFLGRGTFGQ VVKCWKRGTN EIVAIKILKN HPSYARQGQI EVSILARLST
260 270 280 290 300
ENADEYNFVR AYECFQHRNH TCLVFEMLEQ NLYDFLKQNK FSPLPLKVIR
310 320 330 340 350
PVLQQVATAL KKLKSLGLIH ADLKPENIML VDPVRQPYRV KVIDFGSASH
360 370 380 390 400
VSKTVCSTYL QSRYYRAPEI ILGLPFCEAI DMWSLGCVIA ELFLGWPLYP
410 420 430 440 450
GALEYDQIRY ISQTQGLPGE QLLNVGTKST RFFCRETDMS HSGWRLKTLE
460 470 480 490 500
EHEAETGMKS KEARKYIFNS LDDIVHVNTV MDLEGSDLLA EKADRREFVS
510 520 530 540 550
LLKKMLLIDA DLRITPIETL NHPFVSMKHL LDFPHSSHVK SCFHIMDICK
560 570 580 590 600
SPSSCETNNH SKMSLLRPVA SNGTAALAAN FTKVGTLRSQ ALTTSAHSVV
610 620 630 640 650
HHGIPLQAGT AQFGCGDAFH QTLIICPPAI QGIPAAHGKP TSYSIRVDNT
660 670 680 690 700
VPLVTQAPAV QPLQIRPGVL SQTWSGRTQQ MLIPAWQQVT PMAPAAATLT
710 720 730 740 750
SEGMAGSQRL GDWGKMIPHS NHYNSVMPPP LLTNQITLSA PQPISVGIAH
760 770 780 790 800
VVWPQPATTK KNKLCQNRSN SLQNTNVPHS AFISPKIISG KEVEEVSCVE
810 820 830 840 850
TQDNHTSEGE ARTCHEASVR QDSSVSDKQR QTIIIADSPS PAVSVITISS
860 870 880 890 900
DTDDEETSPR PSLRECKGSL DCEACQSTLN IDRMCSLSSP DSTLSTSSSG
910 920 930 940 950
QSSPSPCKRP NSMSDDEQES GCETVDGSPT SDSSGHDSPF AESSFVEDTP
960 970 980 990 1000
QNPELGTCAG TEAKPALSTA VEPPVGTERG LNVDAHMANT DSTCQPLTKG
1010 1020 1030 1040 1050
QPAPGKLNQP SASAARQQKP TSAFQQQHLN LSQVQHFGTG HQEWNGNFGH
1060 1070 1080 1090 1100
RRQQAYIPTS VTSNPFTLSH GSPNHTAVHA HLAGSAHLGG QPTLLPYPPS
1110 1120 1130 1140 1150
TALSSAAPVA HLLASPCTSR PMLQHPTYNI SHPSGIVHQV PVGINPRLLP
1160 1170 1180 1190
SPTIHQTQYK PIFPPHSYIA ASPAYTGFPL SPTKLSQYPY M
Length:1,191
Mass (Da):129,744
Last modified:November 1, 1998 - v1
Checksum:i0C2D05ED947A53ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036959 mRNA. Translation: AAC35249.1.
PIRiT14154.
RefSeqiNP_113975.1. NM_031787.1.
UniGeneiRn.8099.

Genome annotation databases

GeneIDi83617.
KEGGirno:83617.
UCSCiRGD:619884. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036959 mRNA. Translation: AAC35249.1.
PIRiT14154.
RefSeqiNP_113975.1. NM_031787.1.
UniGeneiRn.8099.

3D structure databases

ProteinModelPortaliO88850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-5932N.
STRINGi10116.ENSRNOP00000015775.

PTM databases

PhosphoSiteiO88850.

Proteomic databases

PaxDbiO88850.
PRIDEiO88850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83617.
KEGGirno:83617.
UCSCiRGD:619884. rat.

Organism-specific databases

CTDi10114.
RGDi619884. Hipk3.

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOVERGENiHBG051908.
InParanoidiO88850.
KOiK08826.

Miscellaneous databases

NextBioi616173.
PROiO88850.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Activation of androgen receptor function by a novel nuclear protein kinase."
    Moilanen A.-M., Karvonen U., Poukka H., Jaenne O.A., Palvimo J.J.
    Mol. Biol. Cell 9:2527-2543(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-226; SER-357 AND TYR-359, AUTOPHOSPHORYLATION, INTERACTION WITH AR, FUNCTION.
    Tissue: Testis.

Entry informationi

Entry nameiHIPK3_RAT
AccessioniPrimary (citable) accession number: O88850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.