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O88848

- ARL6_MOUSE

UniProt

O88848 - ARL6_MOUSE

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Protein
ADP-ribosylation factor-like protein 6
Gene
Arl6, Bbs3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Magnesium By similarity
Binding sitei50 – 501GTP By similarity
Binding sitei72 – 721GTP; via amide nitrogen By similarity
Binding sitei164 – 1641GTP; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP By similarity
Nucleotide bindingi69 – 735GTP By similarity
Nucleotide bindingi130 – 1334GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. phospholipid binding Source: UniProtKB
  4. protein binding Source: MGI

GO - Biological processi

  1. Ras protein signal transduction Source: MGI
  2. Wnt signaling pathway Source: Ensembl
  3. cilium assembly Source: UniProtKB
  4. fat cell differentiation Source: BHF-UCL
  5. protein ADP-ribosylation Source: MGI
  6. protein polymerization Source: UniProtKB
  7. protein targeting to membrane Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 6
Gene namesi
Name:Arl6
Synonyms:Bbs3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1927136. Arl6.

Subcellular locationi

Cell projectioncilium membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmcytoskeletoncilium axoneme By similarity. Cytoplasmcytoskeletoncilium basal body By similarity
Note: Appears in a pattern of punctae flanking the microtubule axoneme that likely correspond to small membrane-associated patches. Localizes to the so-called ciliary gate where vesicles carrying ciliary cargo fuse with the membrane By similarity.

GO - Cellular componenti

  1. axonemal microtubule Source: UniProtKB
  2. axoneme Source: UniProtKB
  3. ciliary membrane Source: UniProtKB-SubCell
  4. cytoplasm Source: MGI
  5. membrane Source: MGI
  6. membrane coat Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Isoform 2 deficient mice present a disruption of the normal photoreceptor architecture.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Reviewed prediction
Chaini2 – 186185ADP-ribosylation factor-like protein 6
PRO_0000207473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Reviewed prediction

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiO88848.
PaxDbiO88848.
PRIDEiO88848.

PTM databases

PhosphoSiteiO88848.

Expressioni

Tissue specificityi

Most abundant in brain and kidney. Expressed in heart and eye. Isoform 2 is expressed only in the retina.1 Publication

Gene expression databases

ArrayExpressiO88848.
BgeeiO88848.
CleanExiMM_ARL6.
GenevestigatoriO88848.

Interactioni

Subunit structurei

Interacts with SEC61B, ARL6IP1, ARL6IP2, ARL6IP3, ARL6IP4 ARL6IP5 and ARL6IP6. Interacts (GTP-bound form) with the BBSome a complex that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10 By similarity.

Protein-protein interaction databases

IntActiO88848. 10 interactions.
STRINGi10090.ENSMUSP00000123287.

Structurei

3D structure databases

ProteinModelPortaliO88848.
SMRiO88848. Positions 16-182.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00750000117531.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ3TY77.
KOiK07951.
OMAiDIKHRRL.
OrthoDBiEOG78WKTS.
PhylomeDBiO88848.
TreeFamiTF105466.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88848-1) [UniParc]FASTAAdd to Basket

Also known as: Bbs3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGLLDRLSGL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQDIVPT    50
IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKDGQAI IFVIDSSDKL 100
RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDSVTS VKVSQLLCLE 150
SIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQAVKT 186
Length:186
Mass (Da):20,959
Last modified:November 1, 1998 - v1
Checksum:i4F26947318977386
GO
Isoform 2 (identifier: O88848-2) [UniParc]FASTAAdd to Basket

Also known as: Bbs3l

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: DQIQAVKT → EKTVQSDPSCEDVKR

Show »
Length:193
Mass (Da):21,778
Checksum:iFF91E45A2D785B7C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei179 – 1868DQIQAVKT → EKTVQSDPSCEDVKR in isoform 2.
VSP_040512

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031903 mRNA. Translation: AAC62194.1.
AK158832 mRNA. Translation: BAE34686.1.
AK003778 mRNA. Translation: BAB22990.1.
CCDSiCCDS28259.1. [O88848-1]
RefSeqiNP_062639.3. NM_019665.3. [O88848-1]
XP_006522461.1. XM_006522398.1. [O88848-2]
UniGeneiMm.392505.

Genome annotation databases

EnsembliENSMUST00000023405; ENSMUSP00000023405; ENSMUSG00000022722. [O88848-1]
ENSMUST00000099646; ENSMUSP00000097238; ENSMUSG00000022722. [O88848-2]
ENSMUST00000118438; ENSMUSP00000113127; ENSMUSG00000022722. [O88848-2]
GeneIDi56297.
KEGGimmu:56297.
UCSCiuc007zpl.2. mouse. [O88848-1]
uc007zpm.2. mouse. [O88848-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031903 mRNA. Translation: AAC62194.1 .
AK158832 mRNA. Translation: BAE34686.1 .
AK003778 mRNA. Translation: BAB22990.1 .
CCDSi CCDS28259.1. [O88848-1 ]
RefSeqi NP_062639.3. NM_019665.3. [O88848-1 ]
XP_006522461.1. XM_006522398.1. [O88848-2 ]
UniGenei Mm.392505.

3D structure databases

ProteinModelPortali O88848.
SMRi O88848. Positions 16-182.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88848. 10 interactions.
STRINGi 10090.ENSMUSP00000123287.

PTM databases

PhosphoSitei O88848.

Proteomic databases

MaxQBi O88848.
PaxDbi O88848.
PRIDEi O88848.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023405 ; ENSMUSP00000023405 ; ENSMUSG00000022722 . [O88848-1 ]
ENSMUST00000099646 ; ENSMUSP00000097238 ; ENSMUSG00000022722 . [O88848-2 ]
ENSMUST00000118438 ; ENSMUSP00000113127 ; ENSMUSG00000022722 . [O88848-2 ]
GeneIDi 56297.
KEGGi mmu:56297.
UCSCi uc007zpl.2. mouse. [O88848-1 ]
uc007zpm.2. mouse. [O88848-2 ]

Organism-specific databases

CTDi 84100.
MGIi MGI:1927136. Arl6.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00750000117531.
HOGENOMi HOG000163691.
HOVERGENi HBG002073.
InParanoidi Q3TY77.
KOi K07951.
OMAi DIKHRRL.
OrthoDBi EOG78WKTS.
PhylomeDBi O88848.
TreeFami TF105466.

Miscellaneous databases

NextBioi 312222.
PROi O88848.
SOURCEi Search...

Gene expression databases

ArrayExpressi O88848.
Bgeei O88848.
CleanExi MM_ARL6.
Genevestigatori O88848.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit."
    Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S., Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.
    FEBS Lett. 459:69-74(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Visual cortex.
  3. "Identification and functional analysis of the vision-specific BBS3 (ARL6) long isoform."
    Pretorius P.R., Baye L.M., Nishimura D.Y., Searby C.C., Bugge K., Yang B., Mullins R.F., Stone E.M., Sheffield V.C., Slusarski D.C.
    PLoS Genet. 6:E1000884-E1000884(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiARL6_MOUSE
AccessioniPrimary (citable) accession number: O88848
Secondary accession number(s): Q3TY77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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