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Protein

ADP-ribosylation factor-like protein 6

Gene

Arl6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in membrane protein trafficking at the base of the ciliary organelle (By similarity). Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). Together with BBS1, is necessary for correct trafficking of PKD1 to primary cilia (PubMed:24939912). Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation (By similarity). May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade (By similarity). Isoform 2 may be required for proper retinal function and organization (PubMed:20333246).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501MagnesiumBy similarity
Binding sitei50 – 501GTPBy similarity
Binding sitei72 – 721GTP; via amide nitrogenBy similarity
Binding sitei164 – 1641GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi69 – 735GTPBy similarity
Nucleotide bindingi130 – 1334GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • brain development Source: MGI
  • cilium assembly Source: UniProtKB
  • fat cell differentiation Source: BHF-UCL
  • intermembrane transport Source: MGI
  • protein ADP-ribosylation Source: MGI
  • protein localization to ciliary membrane Source: MGI
  • protein localization to cilium Source: MGI
  • protein localization to nonmotile primary cilium Source: UniProtKB
  • protein polymerization Source: UniProtKB
  • protein targeting to membrane Source: UniProtKB
  • Ras protein signal transduction Source: MGI
  • regulation of smoothened signaling pathway Source: MGI
  • retina layer formation Source: MGI
  • Wnt signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_362338. BBSome-mediated cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 6
Gene namesi
Name:Arl6
Synonyms:Bbs3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1927136. Arl6.

Subcellular locationi

GO - Cellular componenti

  • axonemal microtubule Source: UniProtKB
  • axoneme Source: UniProtKB
  • ciliary membrane Source: UniProtKB-SubCell
  • cilium Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • membrane coat Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Isoform 2 deficient mice present a disruption of the normal photoreceptor architecture.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 186185ADP-ribosylation factor-like protein 6PRO_0000207473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiO88848.
PaxDbiO88848.
PRIDEiO88848.

PTM databases

PhosphoSiteiO88848.

Expressioni

Tissue specificityi

Most abundant in brain and kidney. Expressed in heart and eye. Isoform 2 is expressed only in the retina.1 Publication

Gene expression databases

BgeeiO88848.
CleanExiMM_ARL6.
ExpressionAtlasiO88848. baseline and differential.
GenevisibleiO88848. MM.

Interactioni

Subunit structurei

Interacts with SEC61B, ARL6IP1, ARL6IP2, ARL6IP3, ARL6IP4 ARL6IP5 and ARL6IP6. Interacts (GTP-bound form) with the BBSome a complex that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts (GTP-free form) with IFT27.By similarity

Protein-protein interaction databases

IntActiO88848. 10 interactions.
STRINGi10090.ENSMUSP00000023405.

Structurei

3D structure databases

ProteinModelPortaliO88848.
SMRiO88848. Positions 16-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00790000122980.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiO88848.
KOiK07951.
OMAiRNLWEHQ.
OrthoDBiEOG78WKTS.
PhylomeDBiO88848.
TreeFamiTF105466.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88848-1) [UniParc]FASTAAdd to basket

Also known as: Bbs3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLLDRLSGL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQDIVPT
60 70 80 90 100
IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKDGQAI IFVIDSSDKL
110 120 130 140 150
RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDSVTS VKVSQLLCLE
160 170 180
SIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQAVKT
Length:186
Mass (Da):20,959
Last modified:November 1, 1998 - v1
Checksum:i4F26947318977386
GO
Isoform 2 (identifier: O88848-2) [UniParc]FASTAAdd to basket

Also known as: Bbs3l

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: DQIQAVKT → EKTVQSDPSCEDVKR

Show »
Length:193
Mass (Da):21,778
Checksum:iFF91E45A2D785B7C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei179 – 1868DQIQAVKT → EKTVQSDPSCEDVKR in isoform 2. 1 PublicationVSP_040512

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031903 mRNA. Translation: AAC62194.1.
AK158832 mRNA. Translation: BAE34686.1.
AK003778 mRNA. Translation: BAB22990.1.
CCDSiCCDS28259.1. [O88848-1]
RefSeqiNP_062639.3. NM_019665.3. [O88848-1]
XP_006522461.1. XM_006522398.2. [O88848-2]
UniGeneiMm.392505.

Genome annotation databases

EnsembliENSMUST00000023405; ENSMUSP00000023405; ENSMUSG00000022722. [O88848-1]
ENSMUST00000099646; ENSMUSP00000097238; ENSMUSG00000022722. [O88848-2]
ENSMUST00000118438; ENSMUSP00000113127; ENSMUSG00000022722. [O88848-2]
GeneIDi56297.
KEGGimmu:56297.
UCSCiuc007zpl.2. mouse. [O88848-1]
uc007zpm.2. mouse. [O88848-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031903 mRNA. Translation: AAC62194.1.
AK158832 mRNA. Translation: BAE34686.1.
AK003778 mRNA. Translation: BAB22990.1.
CCDSiCCDS28259.1. [O88848-1]
RefSeqiNP_062639.3. NM_019665.3. [O88848-1]
XP_006522461.1. XM_006522398.2. [O88848-2]
UniGeneiMm.392505.

3D structure databases

ProteinModelPortaliO88848.
SMRiO88848. Positions 16-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88848. 10 interactions.
STRINGi10090.ENSMUSP00000023405.

PTM databases

PhosphoSiteiO88848.

Proteomic databases

MaxQBiO88848.
PaxDbiO88848.
PRIDEiO88848.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023405; ENSMUSP00000023405; ENSMUSG00000022722. [O88848-1]
ENSMUST00000099646; ENSMUSP00000097238; ENSMUSG00000022722. [O88848-2]
ENSMUST00000118438; ENSMUSP00000113127; ENSMUSG00000022722. [O88848-2]
GeneIDi56297.
KEGGimmu:56297.
UCSCiuc007zpl.2. mouse. [O88848-1]
uc007zpm.2. mouse. [O88848-2]

Organism-specific databases

CTDi84100.
MGIiMGI:1927136. Arl6.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00790000122980.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiO88848.
KOiK07951.
OMAiRNLWEHQ.
OrthoDBiEOG78WKTS.
PhylomeDBiO88848.
TreeFamiTF105466.

Enzyme and pathway databases

ReactomeiREACT_362338. BBSome-mediated cargo-targeting to cilium.

Miscellaneous databases

NextBioi312222.
PROiO88848.
SOURCEiSearch...

Gene expression databases

BgeeiO88848.
CleanExiMM_ARL6.
ExpressionAtlasiO88848. baseline and differential.
GenevisibleiO88848. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit."
    Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S., Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.
    FEBS Lett. 459:69-74(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Visual cortex.
  3. "Identification and functional analysis of the vision-specific BBS3 (ARL6) long isoform."
    Pretorius P.R., Baye L.M., Nishimura D.Y., Searby C.C., Bugge K., Yang B., Mullins R.F., Stone E.M., Sheffield V.C., Slusarski D.C.
    PLoS Genet. 6:E1000884-E1000884(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  4. "Bardet-Biedl syndrome proteins 1 and 3 regulate the ciliary trafficking of polycystic kidney disease 1 protein."
    Su X., Driscoll K., Yao G., Raed A., Wu M., Beales P.L., Zhou J.
    Hum. Mol. Genet. 23:5441-5451(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiARL6_MOUSE
AccessioniPrimary (citable) accession number: O88848
Secondary accession number(s): Q3TY77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.