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Protein

A-kinase anchor protein 10, mitochondrial

Gene

Akap10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase.

GO - Molecular functioni

  • protein kinase A binding Source: MGI

GO - Biological processi

  • protein localization Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 10, mitochondrial
Short name:
AKAP-10
Alternative name(s):
Dual specificity A kinase-anchoring protein 2
Short name:
D-AKAP-2
Protein kinase A-anchoring protein 10
Short name:
PRKA10
Gene namesi
Name:Akap10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890218. Akap10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • mitochondrion Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000003040529 – 662A-kinase anchor protein 10, mitochondrialAdd BLAST634

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei189PhosphoserineBy similarity1
Modified residuei281PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88845.
MaxQBiO88845.
PaxDbiO88845.
PeptideAtlasiO88845.
PRIDEiO88845.

PTM databases

iPTMnetiO88845.
PhosphoSitePlusiO88845.

Expressioni

Tissue specificityi

Highly expressed in testis, kidney and lung, followed by brain, skeletal muscle, liver, spleen and heart. Also expressed in brown adipose tissue and pancreas.

Gene expression databases

BgeeiENSMUSG00000047804.
CleanExiMM_AKAP10.
GenevisibleiO88845. MM.

Interactioni

GO - Molecular functioni

  • protein kinase A binding Source: MGI

Protein-protein interaction databases

IntActiO88845. 2 interactors.
MINTiMINT-1734123.
STRINGi10090.ENSMUSP00000099710.

Structurei

3D structure databases

ProteinModelPortaliO88845.
SMRiO88845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 369RGS 1PROSITE-ProRule annotationAdd BLAST245
Domaini379 – 505RGS 2PROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni634 – 647PKA-RII subunit bindingAdd BLAST14

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Contains 2 RGS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3590. Eukaryota.
ENOG410YH1M. LUCA.
GeneTreeiENSGT00390000015077.
HOGENOMiHOG000273900.
HOVERGENiHBG050469.
InParanoidiO88845.
KOiK16526.
OMAiDSAHSLH.
OrthoDBiEOG091G0962.
PhylomeDBiO88845.
TreeFamiTF105409.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 2 hits.
[Graphical view]
SMARTiSM00315. RGS. 2 hits.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 3 hits.
PROSITEiPS50132. RGS. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88845-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGAGPSPRH SPRALRPDPG PAMSFFRRKV KGKEQEKTLD VKSTKASVAV
60 70 80 90 100
HSPQKSTKNH ALLEAAGPSH VAINAISANM DSFSSSRTAT LKKQPSHMEA
110 120 130 140 150
AHFGDLGRSC LDYQTQETKS SLSKTLEQVL RDTVVLPYFL QFMELRRMEH
160 170 180 190 200
LVKFWLEAES FHSTTWSRIR AHSLNTVKQS SLAEPVSPSK RHETPASSVT
210 220 230 240 250
EALDRRLGDS SSAPLLVTQS EGTDLSSRTQ NPQNHLLLSQ EGHSARSLHR
260 270 280 290 300
EVARTGSHQI PTDSQDSSSR LAVGSRNSCS SPLRELSEKL MKSIEQDAVN
310 320 330 340 350
TFTKYISPDA AKPIPITEAM RNDIIAKICG EDGQVDPNCF VLAQAVVFSA
360 370 380 390 400
MEQEHFSEFL RSHHFCKYQI EVLTSGTVYL ADILFCESAL FYFSEYMEKE
410 420 430 440 450
DAVNILQFWL AADNFQSQLA AKKGQYDGQE AQNDAMILYD KYFSLQATHP
460 470 480 490 500
LGFDDVVRLE IESNICREGG PLPNCFTTPL RQAWTTMEKV FLPGFLSSNL
510 520 530 540 550
YYKYLNDLIH SVRGDEFLGG NVSLAAHGSV CLPEESHSGG SDGSTAQSSV
560 570 580 590 600
KKASIKILKN FDEAIIVDAA SLDPESLYQR TYAGKMSFGR VSDLGQFIRE
610 620 630 640 650
SEPEPDVKKS KGFMFSQAMK KWVQGNTDEA QEELAWKIAK MIVSDVMQQA
660
HHDQPLEKST KL
Length:662
Mass (Da):73,632
Last modified:July 19, 2005 - v3
Checksum:iCB31AF0DF13B3AE6
GO
Isoform 2 (identifier: O88845-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     585-662: Missing.

Note: No experimental confirmation available.
Show »
Length:505
Mass (Da):56,292
Checksum:i17F970A1086B58FC
GO
Isoform 3 (identifier: O88845-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     548-560: SSVKKASIKILKN → VLANVTLSMGWEA
     561-662: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):62,030
Checksum:iB87D46DF993C42D1
GO

Sequence cautioni

The sequence AAC61898 differs from that shown. Reason: Frameshift at positions 165 and 225.Curated
The sequence AAH54105 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177V → S (PubMed:15489334).Curated1
Sequence conflicti226S → G (PubMed:9326583).Curated1
Sequence conflicti343 – 344AQ → DT in AAC61898 (PubMed:9326583).Curated2
Sequence conflicti405I → V in AAH54105 (PubMed:15489334).Curated1
Sequence conflicti649Q → P in AAH54105 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0148731 – 79Missing in isoform 2. CuratedAdd BLAST79
Alternative sequenceiVSP_014874548 – 560SSVKK…KILKN → VLANVTLSMGWEA in isoform 3. CuratedAdd BLAST13
Alternative sequenceiVSP_014875561 – 662Missing in isoform 3. CuratedAdd BLAST102
Alternative sequenceiVSP_014876585 – 662Missing in isoform 2. CuratedAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049399 mRNA. Translation: BAC33735.1.
AL646042 Genomic DNA. Translation: CAI25996.1.
AL646042 Genomic DNA. Translation: CAI25997.1.
AL646042 Genomic DNA. Translation: CAI25998.1.
AF021833 mRNA. Translation: AAC61898.1. Frameshift.
BC054105 mRNA. Translation: AAH54105.1. Different initiation.
CCDSiCCDS24821.1. [O88845-1]
RefSeqiNP_064305.2. NM_019921.3. [O88845-1]
UniGeneiMm.274404.

Genome annotation databases

EnsembliENSMUST00000058173; ENSMUSP00000054418; ENSMUSG00000047804. [O88845-2]
ENSMUST00000102650; ENSMUSP00000099710; ENSMUSG00000047804. [O88845-1]
ENSMUST00000108710; ENSMUSP00000104350; ENSMUSG00000047804. [O88845-3]
GeneIDi56697.
KEGGimmu:56697.
UCSCiuc007jii.2. mouse. [O88845-1]
uc007jik.2. mouse. [O88845-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049399 mRNA. Translation: BAC33735.1.
AL646042 Genomic DNA. Translation: CAI25996.1.
AL646042 Genomic DNA. Translation: CAI25997.1.
AL646042 Genomic DNA. Translation: CAI25998.1.
AF021833 mRNA. Translation: AAC61898.1. Frameshift.
BC054105 mRNA. Translation: AAH54105.1. Different initiation.
CCDSiCCDS24821.1. [O88845-1]
RefSeqiNP_064305.2. NM_019921.3. [O88845-1]
UniGeneiMm.274404.

3D structure databases

ProteinModelPortaliO88845.
SMRiO88845.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88845. 2 interactors.
MINTiMINT-1734123.
STRINGi10090.ENSMUSP00000099710.

PTM databases

iPTMnetiO88845.
PhosphoSitePlusiO88845.

Proteomic databases

EPDiO88845.
MaxQBiO88845.
PaxDbiO88845.
PeptideAtlasiO88845.
PRIDEiO88845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058173; ENSMUSP00000054418; ENSMUSG00000047804. [O88845-2]
ENSMUST00000102650; ENSMUSP00000099710; ENSMUSG00000047804. [O88845-1]
ENSMUST00000108710; ENSMUSP00000104350; ENSMUSG00000047804. [O88845-3]
GeneIDi56697.
KEGGimmu:56697.
UCSCiuc007jii.2. mouse. [O88845-1]
uc007jik.2. mouse. [O88845-3]

Organism-specific databases

CTDi11216.
MGIiMGI:1890218. Akap10.

Phylogenomic databases

eggNOGiKOG3590. Eukaryota.
ENOG410YH1M. LUCA.
GeneTreeiENSGT00390000015077.
HOGENOMiHOG000273900.
HOVERGENiHBG050469.
InParanoidiO88845.
KOiK16526.
OMAiDSAHSLH.
OrthoDBiEOG091G0962.
PhylomeDBiO88845.
TreeFamiTF105409.

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiO88845.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047804.
CleanExiMM_AKAP10.
GenevisibleiO88845. MM.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 2 hits.
[Graphical view]
SMARTiSM00315. RGS. 2 hits.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 3 hits.
PROSITEiPS50132. RGS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKA10_MOUSE
AccessioniPrimary (citable) accession number: O88845
Secondary accession number(s): Q5SUB5
, Q5SUB7, Q7TPE7, Q8BQL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.