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Protein

A-kinase anchor protein 10, mitochondrial

Gene

Akap10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase.

GO - Molecular functioni

  • protein kinase A binding Source: MGI

GO - Biological processi

  • protein localization Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 10, mitochondrial
Short name:
AKAP-10
Alternative name(s):
Dual specificity A kinase-anchoring protein 2
Short name:
D-AKAP-2
Protein kinase A-anchoring protein 10
Short name:
PRKA10
Gene namesi
Name:Akap10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890218 Akap10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000003040529 – 662A-kinase anchor protein 10, mitochondrialAdd BLAST634

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei189PhosphoserineBy similarity1
Modified residuei281PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88845
MaxQBiO88845
PaxDbiO88845
PeptideAtlasiO88845
PRIDEiO88845

PTM databases

iPTMnetiO88845
PhosphoSitePlusiO88845

Expressioni

Tissue specificityi

Highly expressed in testis, kidney and lung, followed by brain, skeletal muscle, liver, spleen and heart. Also expressed in brown adipose tissue and pancreas.

Gene expression databases

BgeeiENSMUSG00000047804
CleanExiMM_AKAP10
GenevisibleiO88845 MM

Interactioni

GO - Molecular functioni

  • protein kinase A binding Source: MGI

Protein-protein interaction databases

IntActiO88845, 2 interactors
MINTiO88845
STRINGi10090.ENSMUSP00000099710

Structurei

3D structure databases

ProteinModelPortaliO88845
SMRiO88845
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 369RGS 1PROSITE-ProRule annotationAdd BLAST245
Domaini379 – 505RGS 2PROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni634 – 647PKA-RII subunit bindingAdd BLAST14

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3590 Eukaryota
ENOG410YH1M LUCA
GeneTreeiENSGT00390000015077
HOGENOMiHOG000273900
HOVERGENiHBG050469
InParanoidiO88845
KOiK16526
OMAiEQDAVNT
OrthoDBiEOG091G0962
PhylomeDBiO88845
TreeFamiTF105409

Family and domain databases

CDDicd12804 AKAP10_AKB, 1 hit
InterProiView protein in InterPro
IPR037719 AKAP10_AKB_dom
IPR016137 RGS
IPR036305 RGS_sf
PfamiView protein in Pfam
PF00615 RGS, 2 hits
SMARTiView protein in SMART
SM00315 RGS, 2 hits
SUPFAMiSSF48097 SSF48097, 3 hits
PROSITEiView protein in PROSITE
PS50132 RGS, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88845-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGAGPSPRH SPRALRPDPG PAMSFFRRKV KGKEQEKTLD VKSTKASVAV
60 70 80 90 100
HSPQKSTKNH ALLEAAGPSH VAINAISANM DSFSSSRTAT LKKQPSHMEA
110 120 130 140 150
AHFGDLGRSC LDYQTQETKS SLSKTLEQVL RDTVVLPYFL QFMELRRMEH
160 170 180 190 200
LVKFWLEAES FHSTTWSRIR AHSLNTVKQS SLAEPVSPSK RHETPASSVT
210 220 230 240 250
EALDRRLGDS SSAPLLVTQS EGTDLSSRTQ NPQNHLLLSQ EGHSARSLHR
260 270 280 290 300
EVARTGSHQI PTDSQDSSSR LAVGSRNSCS SPLRELSEKL MKSIEQDAVN
310 320 330 340 350
TFTKYISPDA AKPIPITEAM RNDIIAKICG EDGQVDPNCF VLAQAVVFSA
360 370 380 390 400
MEQEHFSEFL RSHHFCKYQI EVLTSGTVYL ADILFCESAL FYFSEYMEKE
410 420 430 440 450
DAVNILQFWL AADNFQSQLA AKKGQYDGQE AQNDAMILYD KYFSLQATHP
460 470 480 490 500
LGFDDVVRLE IESNICREGG PLPNCFTTPL RQAWTTMEKV FLPGFLSSNL
510 520 530 540 550
YYKYLNDLIH SVRGDEFLGG NVSLAAHGSV CLPEESHSGG SDGSTAQSSV
560 570 580 590 600
KKASIKILKN FDEAIIVDAA SLDPESLYQR TYAGKMSFGR VSDLGQFIRE
610 620 630 640 650
SEPEPDVKKS KGFMFSQAMK KWVQGNTDEA QEELAWKIAK MIVSDVMQQA
660
HHDQPLEKST KL
Length:662
Mass (Da):73,632
Last modified:July 19, 2005 - v3
Checksum:iCB31AF0DF13B3AE6
GO
Isoform 2 (identifier: O88845-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     585-662: Missing.

Note: No experimental confirmation available.
Show »
Length:505
Mass (Da):56,292
Checksum:i17F970A1086B58FC
GO
Isoform 3 (identifier: O88845-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     548-560: SSVKKASIKILKN → VLANVTLSMGWEA
     561-662: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):62,030
Checksum:iB87D46DF993C42D1
GO

Sequence cautioni

The sequence AAC61898 differs from that shown. Reason: Frameshift at positions 165 and 225.Curated
The sequence AAH54105 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177V → S (PubMed:15489334).Curated1
Sequence conflicti226S → G (PubMed:9326583).Curated1
Sequence conflicti343 – 344AQ → DT in AAC61898 (PubMed:9326583).Curated2
Sequence conflicti405I → V in AAH54105 (PubMed:15489334).Curated1
Sequence conflicti649Q → P in AAH54105 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0148731 – 79Missing in isoform 2. CuratedAdd BLAST79
Alternative sequenceiVSP_014874548 – 560SSVKK…KILKN → VLANVTLSMGWEA in isoform 3. CuratedAdd BLAST13
Alternative sequenceiVSP_014875561 – 662Missing in isoform 3. CuratedAdd BLAST102
Alternative sequenceiVSP_014876585 – 662Missing in isoform 2. CuratedAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049399 mRNA Translation: BAC33735.1
AL646042 Genomic DNA No translation available.
AF021833 mRNA Translation: AAC61898.1 Frameshift.
BC054105 mRNA Translation: AAH54105.1 Different initiation.
CCDSiCCDS24821.1 [O88845-1]
RefSeqiNP_064305.2, NM_019921.3 [O88845-1]
UniGeneiMm.274404

Genome annotation databases

EnsembliENSMUST00000058173; ENSMUSP00000054418; ENSMUSG00000047804 [O88845-2]
ENSMUST00000102650; ENSMUSP00000099710; ENSMUSG00000047804 [O88845-1]
ENSMUST00000108710; ENSMUSP00000104350; ENSMUSG00000047804 [O88845-3]
GeneIDi56697
KEGGimmu:56697
UCSCiuc007jii.2 mouse [O88845-1]
uc007jik.2 mouse [O88845-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAKA10_MOUSE
AccessioniPrimary (citable) accession number: O88845
Secondary accession number(s): Q5SUB5
, Q5SUB7, Q7TPE7, Q8BQL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 19, 2005
Last modified: May 23, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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