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Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

Idh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateBy similarity1
Binding sitei82NADPBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Sitei139Critical for catalysisBy similarity1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1
Binding sitei260NADPBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPBy similarity3
Nucleotide bindingi310 – 315NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: UniProtKB
  • female gonad development Source: Ensembl
  • glutathione metabolic process Source: MGI
  • glyoxylate cycle Source: UniProtKB-KW
  • isocitrate metabolic process Source: UniProtKB
  • regulation of phospholipid biosynthetic process Source: MGI
  • regulation of phospholipid catabolic process Source: MGI
  • response to oxidative stress Source: MGI
  • response to steroid hormone Source: Ensembl
  • tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.42. 3474.
ReactomeiR-MMU-389542. NADPH regeneration.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:Idh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:96413. Idh1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000835782 – 414Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei42PhosphotyrosineBy similarity1
Modified residuei81N6-acetyllysineCombined sources1
Modified residuei126N6-succinyllysineCombined sources1
Modified residuei224N6-acetyllysineCombined sources1
Modified residuei233N6-acetyllysineCombined sources1
Modified residuei243N6-acetyllysineCombined sources1
Modified residuei321N6-acetyllysineBy similarity1
Modified residuei389PhosphoserineCombined sources1
Modified residuei400N6-succinyllysineCombined sources1

Post-translational modificationi

Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88844.
MaxQBiO88844.
PaxDbiO88844.
PeptideAtlasiO88844.
PRIDEiO88844.

2D gel databases

COMPLUYEAST-2DPAGEO88844.
REPRODUCTION-2DPAGEO88844.
SWISS-2DPAGEO88844.

PTM databases

iPTMnetiO88844.
PhosphoSitePlusiO88844.
SwissPalmiO88844.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025950.
CleanExiMM_IDH1.
ExpressionAtlasiO88844. baseline and differential.
GenevisibleiO88844. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200510. 1 interactor.
IntActiO88844. 5 interactors.
MINTiMINT-1859161.
STRINGi10090.ENSMUSP00000095316.

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Helixi17 – 30Combined sources14
Turni31 – 34Combined sources4
Beta strandi35 – 43Combined sources9
Helixi46 – 51Combined sources6
Turni52 – 54Combined sources3
Helixi55 – 67Combined sources13
Beta strandi68 – 72Combined sources5
Helixi80 – 86Combined sources7
Helixi95 – 103Combined sources9
Beta strandi106 – 111Combined sources6
Beta strandi128 – 133Combined sources6
Helixi137 – 140Combined sources4
Beta strandi142 – 146Combined sources5
Beta strandi148 – 162Combined sources15
Beta strandi165 – 172Combined sources8
Beta strandi177 – 185Combined sources9
Helixi186 – 203Combined sources18
Beta strandi207 – 211Combined sources5
Turni213 – 215Combined sources3
Helixi219 – 234Combined sources16
Helixi236 – 241Combined sources6
Beta strandi246 – 250Combined sources5
Helixi251 – 260Combined sources10
Beta strandi265 – 269Combined sources5
Helixi271 – 285Combined sources15
Beta strandi290 – 296Combined sources7
Beta strandi303 – 306Combined sources4
Helixi313 – 320Combined sources8
Helixi330 – 347Combined sources18
Helixi350 – 368Combined sources19
Helixi374 – 381Combined sources8
Helixi383 – 385Combined sources3
Helixi388 – 390Combined sources3
Helixi394 – 412Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CMJX-ray1.99A/B4-413[»]
2CMVX-ray2.52A/B4-413[»]
ProteinModelPortaliO88844.
SMRiO88844.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88844.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiO88844.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG091G06IY.
TreeFamiTF300428.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKIQGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GTQKVTYMVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFE AQKICYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFAKALE DVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Length:414
Mass (Da):46,674
Last modified:July 27, 2011 - v2
Checksum:iC7FEF315D042C44C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243K → N in AAD02919 (PubMed:9866202).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020039 mRNA. Translation: AAD02919.1.
AK149019 mRNA. Translation: BAE28720.1.
AK151212 mRNA. Translation: BAE30207.1.
AK159173 mRNA. Translation: BAE34873.1.
AK160896 mRNA. Translation: BAE36075.1.
AK167158 mRNA. Translation: BAE39299.1.
CH466548 Genomic DNA. Translation: EDL00223.1.
CH466548 Genomic DNA. Translation: EDL00225.1.
CCDSiCCDS15016.1.
RefSeqiNP_001104790.1. NM_001111320.1.
NP_034627.3. NM_010497.3.
UniGeneiMm.9925.

Genome annotation databases

EnsembliENSMUST00000097709; ENSMUSP00000095316; ENSMUSG00000025950.
ENSMUST00000169032; ENSMUSP00000127307; ENSMUSG00000025950.
GeneIDi15926.
KEGGimmu:15926.
UCSCiuc007bhn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020039 mRNA. Translation: AAD02919.1.
AK149019 mRNA. Translation: BAE28720.1.
AK151212 mRNA. Translation: BAE30207.1.
AK159173 mRNA. Translation: BAE34873.1.
AK160896 mRNA. Translation: BAE36075.1.
AK167158 mRNA. Translation: BAE39299.1.
CH466548 Genomic DNA. Translation: EDL00223.1.
CH466548 Genomic DNA. Translation: EDL00225.1.
CCDSiCCDS15016.1.
RefSeqiNP_001104790.1. NM_001111320.1.
NP_034627.3. NM_010497.3.
UniGeneiMm.9925.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CMJX-ray1.99A/B4-413[»]
2CMVX-ray2.52A/B4-413[»]
ProteinModelPortaliO88844.
SMRiO88844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200510. 1 interactor.
IntActiO88844. 5 interactors.
MINTiMINT-1859161.
STRINGi10090.ENSMUSP00000095316.

PTM databases

iPTMnetiO88844.
PhosphoSitePlusiO88844.
SwissPalmiO88844.

2D gel databases

COMPLUYEAST-2DPAGEO88844.
REPRODUCTION-2DPAGEO88844.
SWISS-2DPAGEO88844.

Proteomic databases

EPDiO88844.
MaxQBiO88844.
PaxDbiO88844.
PeptideAtlasiO88844.
PRIDEiO88844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097709; ENSMUSP00000095316; ENSMUSG00000025950.
ENSMUST00000169032; ENSMUSP00000127307; ENSMUSG00000025950.
GeneIDi15926.
KEGGimmu:15926.
UCSCiuc007bhn.2. mouse.

Organism-specific databases

CTDi3417.
MGIiMGI:96413. Idh1.

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiO88844.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG091G06IY.
TreeFamiTF300428.

Enzyme and pathway databases

BRENDAi1.1.1.42. 3474.
ReactomeiR-MMU-389542. NADPH regeneration.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiO88844.
PROiO88844.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025950.
CleanExiMM_IDH1.
ExpressionAtlasiO88844. baseline and differential.
GenevisibleiO88844. MM.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDHC_MOUSE
AccessioniPrimary (citable) accession number: O88844
Secondary accession number(s): Q3UAV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.