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O88844

- IDHC_MOUSE

UniProt

O88844 - IDHC_MOUSE

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Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

Idh1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateBy similarity
Binding sitei82 – 821NADPBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Sitei139 – 1391Critical for catalysisBy similarity
Sitei212 – 2121Critical for catalysisBy similarity
Metal bindingi252 – 2521Magnesium or manganeseBy similarity
Binding sitei260 – 2601NADPBy similarity
Metal bindingi275 – 2751Magnesium or manganeseBy similarity
Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPBy similarity
Nucleotide bindingi310 – 3156NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. NAD binding Source: InterPro
  4. NADP binding Source: Ensembl

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: UniProtKB
  2. female gonad development Source: Ensembl
  3. glutathione metabolic process Source: MGI
  4. glyoxylate cycle Source: UniProtKB-KW
  5. isocitrate metabolic process Source: UniProtKB
  6. response to oxidative stress Source: MGI
  7. response to steroid hormone Source: Ensembl
  8. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_198632. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:Idh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:96413. Idh1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. mitochondrion Source: MGI
  4. peroxisome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 414413Isocitrate dehydrogenase [NADP] cytoplasmicPRO_0000083578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei81 – 811N6-acetyllysine1 Publication
Modified residuei126 – 1261N6-succinyllysine1 Publication
Modified residuei224 – 2241N6-acetyllysine1 Publication
Modified residuei233 – 2331N6-acetyllysine1 Publication
Modified residuei243 – 2431N6-acetyllysine1 Publication
Modified residuei321 – 3211N6-acetyllysineBy similarity
Modified residuei400 – 4001N6-succinyllysine1 Publication

Post-translational modificationi

Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO88844.
PaxDbiO88844.
PRIDEiO88844.

2D gel databases

COMPLUYEAST-2DPAGEO88844.
REPRODUCTION-2DPAGEO88844.
SWISS-2DPAGEO88844.

PTM databases

PhosphoSiteiO88844.

Expressioni

Gene expression databases

BgeeiO88844.
CleanExiMM_IDH1.
ExpressionAtlasiO88844. baseline and differential.
GenevestigatoriO88844.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO88844. 5 interactions.
MINTiMINT-1859161.

Structurei

Secondary structure

1
414
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410
Helixi17 – 3014
Turni31 – 344
Beta strandi35 – 439
Helixi46 – 516
Turni52 – 543
Helixi55 – 6713
Beta strandi68 – 725
Helixi80 – 867
Helixi95 – 1039
Beta strandi106 – 1116
Beta strandi128 – 1336
Helixi137 – 1404
Beta strandi142 – 1465
Beta strandi148 – 16215
Beta strandi165 – 1728
Beta strandi177 – 1859
Helixi186 – 20318
Beta strandi207 – 2115
Turni213 – 2153
Helixi219 – 23416
Helixi236 – 2416
Beta strandi246 – 2505
Helixi251 – 26010
Beta strandi265 – 2695
Helixi271 – 28515
Beta strandi290 – 2967
Beta strandi303 – 3064
Helixi313 – 3208
Helixi330 – 34718
Helixi350 – 36819
Helixi374 – 3818
Helixi383 – 3853
Helixi388 – 3903
Helixi394 – 41219

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CMJX-ray1.99A/B4-413[»]
2CMVX-ray2.52A/B4-413[»]
ProteinModelPortaliO88844.
SMRiO88844. Positions 4-413.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88844.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0538.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiO88844.
KOiK00031.
OMAiSCGGVAM.
OrthoDBiEOG7QNVKS.
TreeFamiTF300428.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88844-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRKIQGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GTQKVTYMVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFE AQKICYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFAKALE DVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Length:414
Mass (Da):46,674
Last modified:July 27, 2011 - v2
Checksum:iC7FEF315D042C44C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431K → N in AAD02919. (PubMed:9866202)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020039 mRNA. Translation: AAD02919.1.
AK149019 mRNA. Translation: BAE28720.1.
AK151212 mRNA. Translation: BAE30207.1.
AK159173 mRNA. Translation: BAE34873.1.
AK160896 mRNA. Translation: BAE36075.1.
AK167158 mRNA. Translation: BAE39299.1.
CH466548 Genomic DNA. Translation: EDL00223.1.
CH466548 Genomic DNA. Translation: EDL00225.1.
CCDSiCCDS15016.1.
RefSeqiNP_001104790.1. NM_001111320.1.
NP_034627.3. NM_010497.3.
UniGeneiMm.9925.

Genome annotation databases

EnsembliENSMUST00000097709; ENSMUSP00000095316; ENSMUSG00000025950.
ENSMUST00000169032; ENSMUSP00000127307; ENSMUSG00000025950.
GeneIDi15926.
KEGGimmu:15926.
UCSCiuc007bhn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020039 mRNA. Translation: AAD02919.1 .
AK149019 mRNA. Translation: BAE28720.1 .
AK151212 mRNA. Translation: BAE30207.1 .
AK159173 mRNA. Translation: BAE34873.1 .
AK160896 mRNA. Translation: BAE36075.1 .
AK167158 mRNA. Translation: BAE39299.1 .
CH466548 Genomic DNA. Translation: EDL00223.1 .
CH466548 Genomic DNA. Translation: EDL00225.1 .
CCDSi CCDS15016.1.
RefSeqi NP_001104790.1. NM_001111320.1.
NP_034627.3. NM_010497.3.
UniGenei Mm.9925.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CMJ X-ray 1.99 A/B 4-413 [» ]
2CMV X-ray 2.52 A/B 4-413 [» ]
ProteinModelPortali O88844.
SMRi O88844. Positions 4-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88844. 5 interactions.
MINTi MINT-1859161.

PTM databases

PhosphoSitei O88844.

2D gel databases

COMPLUYEAST-2DPAGE O88844.
REPRODUCTION-2DPAGE O88844.
SWISS-2DPAGE O88844.

Proteomic databases

MaxQBi O88844.
PaxDbi O88844.
PRIDEi O88844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000097709 ; ENSMUSP00000095316 ; ENSMUSG00000025950 .
ENSMUST00000169032 ; ENSMUSP00000127307 ; ENSMUSG00000025950 .
GeneIDi 15926.
KEGGi mmu:15926.
UCSCi uc007bhn.2. mouse.

Organism-specific databases

CTDi 3417.
MGIi MGI:96413. Idh1.

Phylogenomic databases

eggNOGi COG0538.
GeneTreei ENSGT00390000012547.
HOGENOMi HOG000019858.
HOVERGENi HBG006119.
InParanoidi O88844.
KOi K00031.
OMAi SCGGVAM.
OrthoDBi EOG7QNVKS.
TreeFami TF300428.

Enzyme and pathway databases

Reactomei REACT_198632. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Miscellaneous databases

EvolutionaryTracei O88844.
NextBioi 288640.
PROi O88844.
SOURCEi Search...

Gene expression databases

Bgeei O88844.
CleanExi MM_IDH1.
ExpressionAtlasi O88844. baseline and differential.
Genevestigatori O88844.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family."
    Nekrutenko A., Hillis D.M., Patton J.C., Bradley R.D., Baker R.J.
    Mol. Biol. Evol. 15:1674-1684(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow, Embryo and Sympathetic ganglion.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Lubec G., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 30-49.
    Tissue: Brain.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-126 AND LYS-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-81; LYS-224; LYS-233 AND LYS-243, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIDHC_MOUSE
AccessioniPrimary (citable) accession number: O88844
Secondary accession number(s): Q3UAV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3