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Protein

FYVE, RhoGEF and PH domain-containing protein 3

Gene

Fgd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri528 – 58457FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • filopodium assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_297947. Rho GTPase cycle.
REACT_299052. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
FYVE, RhoGEF and PH domain-containing protein 3
Gene namesi
Name:Fgd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1353657. Fgd3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733FYVE, RhoGEF and PH domain-containing protein 3PRO_0000080945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241PhosphoserineBy similarity
Modified residuei732 – 7321Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88842.
PaxDbiO88842.
PRIDEiO88842.

PTM databases

PhosphoSiteiO88842.

Expressioni

Tissue specificityi

Detected in adult brain, spleen, lung and skeletal muscle. Detected in embryos from E7 to E17.1 Publication

Gene expression databases

BgeeiO88842.
CleanExiMM_FGD3.
ExpressionAtlasiO88842. baseline and differential.
GenevestigatoriO88842.

Structurei

3D structure databases

ProteinModelPortaliO88842.
SMRiO88842. Positions 140-513, 524-580, 614-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 337185DHPROSITE-ProRule annotationAdd
BLAST
Domaini366 – 465100PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini612 – 711100PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri528 – 58457FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5422.
HOGENOMiHOG000220866.
HOVERGENiHBG007506.
InParanoidiO88842.
OrthoDBiEOG7C2R0M.
PhylomeDBiO88842.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88842-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELGRSSSTP QEEAISPLGV LGTGPSSSPL GKLQALPIGP GAHRGAHSSS
60 70 80 90 100
APAGDSSTRE PSGAMKIPNR DSGIDSPSSS VASENFPCEE SSEGSPSPAI
110 120 130 140 150
LGLPSETASD SRVPQDNPQE EEDSGVGEEP DPKVTLFRPQ EDVSLTQCSD
160 170 180 190 200
PQKLLHIAQE LLHTEEAYVK RLHLLDQVFC TKLTEAGIPL EVTTGIFSNI
210 220 230 240 250
SSIYRFHGQF LLPELQKRIT EEWDTNPRLG DILQKLAPFL KMYGEYVKNF
260 270 280 290 300
DRAMGLVSTW TQRSPQFKDV IHTIQKQEVC GNLTLQHHML EPVQRVPRYE
310 320 330 340 350
LLLKDYLKRL PRDAPDRKDA ERSLELISTA ADHSNAAIRK MEKMHKLLEV
360 370 380 390 400
YEQLGGEEDI VNPANELIKE GSIQKLSAKN GTTQDRHLFL FNNVMLYCVP
410 420 430 440 450
KLRLMGQKLS VREKMDISDL QVQDIVKPNA ACTFIITGRK RSLELQTRTE
460 470 480 490 500
EEKKEWIQVI QATVEKHKQK SETFRAFGGA CSQDEEPTLS PDQPVMSTSS
510 520 530 540 550
VEPAGVADSN GGTPGIESRK SSSKTRRDKE KPGCKSCGET FNSITKRRYR
560 570 580 590 600
CKLCGEVICR KCSEFKAENS KQSRVCRECF LEEPLVPPSP SSETPTELKQ
610 620 630 640 650
NAEKPPSVDP RPSLLCGTLN LSDDGTTWNE VWAAIPESDP QVLDLLAGSQ
660 670 680 690 700
AGRLLYSIPL SGCNITMPDP EEGLEAGCAW KLHQGSQTWW LSAPSTKLQQ
710 720 730
CWLKALGTAV HGDTAGDRPG ASQPQAPAGT DTP
Length:733
Mass (Da):80,624
Last modified:November 1, 1998 - v1
Checksum:i86CDFEBB5DC1E8E3
GO
Isoform 2 (identifier: O88842-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     516-532: IESRKSSSKTRRDKEKP → VSDPHPGISRSERCLGR
     533-733: Missing.

Note: No experimental confirmation available.

Show »
Length:532
Mass (Da):58,758
Checksum:i5BAA48BA0DCA02FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71S → P in BAC34624 (PubMed:16141072).Curated
Sequence conflicti126 – 1261V → M in BAC34624 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei516 – 53217IESRK…DKEKP → VSDPHPGISRSERCLGR in isoform 2. 1 PublicationVSP_013076Add
BLAST
Alternative sequencei533 – 733201Missing in isoform 2. 1 PublicationVSP_013077Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017369 mRNA. Translation: AAC35431.1.
AK051395 mRNA. Translation: BAC34624.1.
BC132340 mRNA. Translation: AAI32341.1.
BC137954 mRNA. Translation: AAI37955.1.
CCDSiCCDS26499.1. [O88842-1]
UniGeneiMm.291089.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017369 mRNA. Translation: AAC35431.1.
AK051395 mRNA. Translation: BAC34624.1.
BC132340 mRNA. Translation: AAI32341.1.
BC137954 mRNA. Translation: AAI37955.1.
CCDSiCCDS26499.1. [O88842-1]
UniGeneiMm.291089.

3D structure databases

ProteinModelPortaliO88842.
SMRiO88842. Positions 140-513, 524-580, 614-711.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO88842.

Proteomic databases

MaxQBiO88842.
PaxDbiO88842.
PRIDEiO88842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1353657. Fgd3.

Phylogenomic databases

eggNOGiCOG5422.
HOGENOMiHOG000220866.
HOVERGENiHBG007506.
InParanoidiO88842.
OrthoDBiEOG7C2R0M.
PhylomeDBiO88842.

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_297947. Rho GTPase cycle.
REACT_299052. G alpha (12/13) signalling events.

Miscellaneous databases

PROiO88842.
SOURCEiSearch...

Gene expression databases

BgeeiO88842.
CleanExiMM_FGD3.
ExpressionAtlasiO88842. baseline and differential.
GenevestigatoriO88842.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and mapping of the mouse Fgd3 gene, a new faciogenital dysplasia (FGD1; Aarskog syndrome) gene homologue."
    Pasteris N.G., Nagata K., Hall A., Gorski J.L.
    Gene 242:237-247(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-732, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFGD3_MOUSE
AccessioniPrimary (citable) accession number: O88842
Secondary accession number(s): A2RT26, Q8BQ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: May 27, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.