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O88842

- FGD3_MOUSE

UniProt

O88842 - FGD3_MOUSE

Protein

FYVE, RhoGEF and PH domain-containing protein 3

Gene

Fgd3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri528 – 58457FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. filopodium assembly Source: UniProtKB

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FYVE, RhoGEF and PH domain-containing protein 3
    Gene namesi
    Name:Fgd3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1353657. Fgd3.

    Subcellular locationi

    Cytoplasm Curated. Cytoplasmcytoskeleton Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 733733FYVE, RhoGEF and PH domain-containing protein 3PRO_0000080945Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241PhosphoserineBy similarity
    Modified residuei732 – 7321Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO88842.
    PRIDEiO88842.

    PTM databases

    PhosphoSiteiO88842.

    Expressioni

    Tissue specificityi

    Detected in adult brain, spleen, lung and skeletal muscle. Detected in embryos from E7 to E17.1 Publication

    Gene expression databases

    ArrayExpressiO88842.
    BgeeiO88842.
    CleanExiMM_FGD3.
    GenevestigatoriO88842.

    Structurei

    3D structure databases

    ProteinModelPortaliO88842.
    SMRiO88842. Positions 140-513, 524-580, 614-711.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 337185DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini366 – 465100PH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini612 – 711100PH 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
    Contains 2 PH domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri528 – 58457FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5422.
    GeneTreeiENSGT00750000117280.
    HOGENOMiHOG000220866.
    HOVERGENiHBG007506.
    InParanoidiA2RT26.
    OrthoDBiEOG7C2R0M.
    PhylomeDBiO88842.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF01363. FYVE. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    SSF57903. SSF57903. 2 hits.
    PROSITEiPS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O88842-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELGRSSSTP QEEAISPLGV LGTGPSSSPL GKLQALPIGP GAHRGAHSSS    50
    APAGDSSTRE PSGAMKIPNR DSGIDSPSSS VASENFPCEE SSEGSPSPAI 100
    LGLPSETASD SRVPQDNPQE EEDSGVGEEP DPKVTLFRPQ EDVSLTQCSD 150
    PQKLLHIAQE LLHTEEAYVK RLHLLDQVFC TKLTEAGIPL EVTTGIFSNI 200
    SSIYRFHGQF LLPELQKRIT EEWDTNPRLG DILQKLAPFL KMYGEYVKNF 250
    DRAMGLVSTW TQRSPQFKDV IHTIQKQEVC GNLTLQHHML EPVQRVPRYE 300
    LLLKDYLKRL PRDAPDRKDA ERSLELISTA ADHSNAAIRK MEKMHKLLEV 350
    YEQLGGEEDI VNPANELIKE GSIQKLSAKN GTTQDRHLFL FNNVMLYCVP 400
    KLRLMGQKLS VREKMDISDL QVQDIVKPNA ACTFIITGRK RSLELQTRTE 450
    EEKKEWIQVI QATVEKHKQK SETFRAFGGA CSQDEEPTLS PDQPVMSTSS 500
    VEPAGVADSN GGTPGIESRK SSSKTRRDKE KPGCKSCGET FNSITKRRYR 550
    CKLCGEVICR KCSEFKAENS KQSRVCRECF LEEPLVPPSP SSETPTELKQ 600
    NAEKPPSVDP RPSLLCGTLN LSDDGTTWNE VWAAIPESDP QVLDLLAGSQ 650
    AGRLLYSIPL SGCNITMPDP EEGLEAGCAW KLHQGSQTWW LSAPSTKLQQ 700
    CWLKALGTAV HGDTAGDRPG ASQPQAPAGT DTP 733
    Length:733
    Mass (Da):80,624
    Last modified:November 1, 1998 - v1
    Checksum:i86CDFEBB5DC1E8E3
    GO
    Isoform 2 (identifier: O88842-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         516-532: IESRKSSSKTRRDKEKP → VSDPHPGISRSERCLGR
         533-733: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:532
    Mass (Da):58,758
    Checksum:i5BAA48BA0DCA02FE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71S → P in BAC34624. (PubMed:16141072)Curated
    Sequence conflicti126 – 1261V → M in BAC34624. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei516 – 53217IESRK…DKEKP → VSDPHPGISRSERCLGR in isoform 2. 1 PublicationVSP_013076Add
    BLAST
    Alternative sequencei533 – 733201Missing in isoform 2. 1 PublicationVSP_013077Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017369 mRNA. Translation: AAC35431.1.
    AK051395 mRNA. Translation: BAC34624.1.
    BC132340 mRNA. Translation: AAI32341.1.
    BC137954 mRNA. Translation: AAI37955.1.
    CCDSiCCDS26499.1. [O88842-1]
    UniGeneiMm.291089.

    Genome annotation databases

    EnsembliENSMUST00000110086; ENSMUSP00000105713; ENSMUSG00000037946.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017369 mRNA. Translation: AAC35431.1 .
    AK051395 mRNA. Translation: BAC34624.1 .
    BC132340 mRNA. Translation: AAI32341.1 .
    BC137954 mRNA. Translation: AAI37955.1 .
    CCDSi CCDS26499.1. [O88842-1 ]
    UniGenei Mm.291089.

    3D structure databases

    ProteinModelPortali O88842.
    SMRi O88842. Positions 140-513, 524-580, 614-711.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O88842.

    Proteomic databases

    PaxDbi O88842.
    PRIDEi O88842.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000110086 ; ENSMUSP00000105713 ; ENSMUSG00000037946 .

    Organism-specific databases

    MGIi MGI:1353657. Fgd3.

    Phylogenomic databases

    eggNOGi COG5422.
    GeneTreei ENSGT00750000117280.
    HOGENOMi HOG000220866.
    HOVERGENi HBG007506.
    InParanoidi A2RT26.
    OrthoDBi EOG7C2R0M.
    PhylomeDBi O88842.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    PROi O88842.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88842.
    Bgeei O88842.
    CleanExi MM_FGD3.
    Genevestigatori O88842.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF01363. FYVE. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00064. FYVE. 1 hit.
    SM00233. PH. 2 hits.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    SSF57903. SSF57903. 2 hits.
    PROSITEi PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and mapping of the mouse Fgd3 gene, a new faciogenital dysplasia (FGD1; Aarskog syndrome) gene homologue."
      Pasteris N.G., Nagata K., Hall A., Gorski J.L.
      Gene 242:237-247(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Spleen.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Spinal ganglion.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-732, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFGD3_MOUSE
    AccessioniPrimary (citable) accession number: O88842
    Secondary accession number(s): A2RT26, Q8BQ72
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3