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O88842 (FGD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FYVE, RhoGEF and PH domain-containing protein 3
Gene names
Name:Fgd3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length733 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Ref.1

Subcellular location

Cytoplasm Probable. Cytoplasmcytoskeleton Probable.

Tissue specificity

Detected in adult brain, spleen, lung and skeletal muscle. Detected in embryos from E7 to E17. Ref.1

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 FYVE-type zinc finger.

Contains 2 PH domains.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionGuanine-nucleotide releasing factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfilopodium assembly

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRho guanyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O88842-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O88842-2)

The sequence of this isoform differs from the canonical sequence as follows:
     516-532: IESRKSSSKTRRDKEKP → VSDPHPGISRSERCLGR
     533-733: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 733733FYVE, RhoGEF and PH domain-containing protein 3
PRO_0000080945

Regions

Domain153 – 337185DH
Domain366 – 465100PH 1
Domain612 – 711100PH 2
Zinc finger528 – 58457FYVE-type

Amino acid modifications

Modified residue1241Phosphoserine By similarity
Modified residue7321Phosphothreonine Ref.4

Natural variations

Alternative sequence516 – 53217IESRK…DKEKP → VSDPHPGISRSERCLGR in isoform 2.
VSP_013076
Alternative sequence533 – 733201Missing in isoform 2.
VSP_013077

Experimental info

Sequence conflict71S → P in BAC34624. Ref.2
Sequence conflict1261V → M in BAC34624. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 86CDFEBB5DC1E8E3

FASTA73380,624
        10         20         30         40         50         60 
MELGRSSSTP QEEAISPLGV LGTGPSSSPL GKLQALPIGP GAHRGAHSSS APAGDSSTRE 

        70         80         90        100        110        120 
PSGAMKIPNR DSGIDSPSSS VASENFPCEE SSEGSPSPAI LGLPSETASD SRVPQDNPQE 

       130        140        150        160        170        180 
EEDSGVGEEP DPKVTLFRPQ EDVSLTQCSD PQKLLHIAQE LLHTEEAYVK RLHLLDQVFC 

       190        200        210        220        230        240 
TKLTEAGIPL EVTTGIFSNI SSIYRFHGQF LLPELQKRIT EEWDTNPRLG DILQKLAPFL 

       250        260        270        280        290        300 
KMYGEYVKNF DRAMGLVSTW TQRSPQFKDV IHTIQKQEVC GNLTLQHHML EPVQRVPRYE 

       310        320        330        340        350        360 
LLLKDYLKRL PRDAPDRKDA ERSLELISTA ADHSNAAIRK MEKMHKLLEV YEQLGGEEDI 

       370        380        390        400        410        420 
VNPANELIKE GSIQKLSAKN GTTQDRHLFL FNNVMLYCVP KLRLMGQKLS VREKMDISDL 

       430        440        450        460        470        480 
QVQDIVKPNA ACTFIITGRK RSLELQTRTE EEKKEWIQVI QATVEKHKQK SETFRAFGGA 

       490        500        510        520        530        540 
CSQDEEPTLS PDQPVMSTSS VEPAGVADSN GGTPGIESRK SSSKTRRDKE KPGCKSCGET 

       550        560        570        580        590        600 
FNSITKRRYR CKLCGEVICR KCSEFKAENS KQSRVCRECF LEEPLVPPSP SSETPTELKQ 

       610        620        630        640        650        660 
NAEKPPSVDP RPSLLCGTLN LSDDGTTWNE VWAAIPESDP QVLDLLAGSQ AGRLLYSIPL 

       670        680        690        700        710        720 
SGCNITMPDP EEGLEAGCAW KLHQGSQTWW LSAPSTKLQQ CWLKALGTAV HGDTAGDRPG 

       730 
ASQPQAPAGT DTP 

« Hide

Isoform 2 [UniParc].

Checksum: 5BAA48BA0DCA02FE
Show »

FASTA53258,758

References

« Hide 'large scale' references
[1]"Isolation, characterization, and mapping of the mouse Fgd3 gene, a new faciogenital dysplasia (FGD1; Aarskog syndrome) gene homologue."
Pasteris N.G., Nagata K., Hall A., Gorski J.L.
Gene 242:237-247(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Spinal ganglion.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-732, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017369 mRNA. Translation: AAC35431.1.
AK051395 mRNA. Translation: BAC34624.1.
BC132340 mRNA. Translation: AAI32341.1.
BC137954 mRNA. Translation: AAI37955.1.
CCDSCCDS26499.1. [O88842-1]
UniGeneMm.291089.

3D structure databases

ProteinModelPortalO88842.
SMRO88842. Positions 140-513, 524-580, 614-711.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO88842.

Proteomic databases

PaxDbO88842.
PRIDEO88842.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000110086; ENSMUSP00000105713; ENSMUSG00000037946.

Organism-specific databases

MGIMGI:1353657. Fgd3.

Phylogenomic databases

eggNOGCOG5422.
GeneTreeENSGT00750000117280.
HOGENOMHOG000220866.
HOVERGENHBG007506.
InParanoidA2RT26.
OrthoDBEOG7C2R0M.
PhylomeDBO88842.

Gene expression databases

ArrayExpressO88842.
BgeeO88842.
CleanExMM_FGD3.
GenevestigatorO88842.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProIPR000219. DH-domain.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO88842.
SOURCESearch...

Entry information

Entry nameFGD3_MOUSE
AccessionPrimary (citable) accession number: O88842
Secondary accession number(s): A2RT26, Q8BQ72
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot