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Protein

SPRY domain-containing SOCS box protein 2

Gene

Spsb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664. Neddylation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 2
Short name:
SSB-2
Alternative name(s):
Gene-rich cluster protein C9
Gene namesi
Name:Spsb2
Synonyms:Grcc9, Ssb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315199. Spsb2.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120Y → A: Loss of interaction with PARW. No effect on interaction with MET. 2 Publications1
Mutagenesisi123 – 125LLG → AAA: Loss of interaction with MET and PARW. 2 Publications3
Mutagenesisi126 – 128SNS → AAA: Loss of interaction with PAWR. 1 Publication3
Mutagenesisi141 – 143YHQ → AAA: Strongly reduced interaction with MET and PARW. 1 Publication3
Mutagenesisi151 – 152QY → AA: Loss of interaction with MET and PARW. 1 Publication2
Mutagenesisi188 – 189GP → AA: Strongly reduced interaction with MET. Loss of interaction with PARW. 1 Publication2
Mutagenesisi198T → A: No loss of interaction with PAWR. 1 Publication1
Mutagenesisi206V → A: Loss of interaction with PARW. No effect on interaction with MET. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL3325309.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384751 – 264SPRY domain-containing SOCS box protein 2Add BLAST264

Proteomic databases

MaxQBiO88838.
PaxDbiO88838.
PRIDEiO88838.

PTM databases

PhosphoSitePlusiO88838.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038451.
ExpressionAtlasiO88838. baseline and differential.
GenevisibleiO88838. MM.

Interactioni

Subunit structurei

Component of the probable ECS(SPSB2) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB2 (By similarity). Interacts with CUL5, RNF7, ELOB and ELOC (By similarity). Interacts with MET (PubMed:16369487). Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAWRQ96IZ06EBI-8820410,EBI-595869From Homo sapiens.

Protein-protein interaction databases

BioGridi200055. 1 interactor.
DIPiDIP-29001N.
IntActiO88838. 2 interactors.
STRINGi10090.ENSMUSP00000041585.

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 34Combined sources6
Helixi40 – 45Combined sources6
Beta strandi47 – 53Combined sources7
Beta strandi57 – 60Combined sources4
Helixi61 – 63Combined sources3
Beta strandi65 – 68Combined sources4
Turni72 – 74Combined sources3
Beta strandi75 – 81Combined sources7
Beta strandi84 – 94Combined sources11
Helixi97 – 99Combined sources3
Beta strandi105 – 109Combined sources5
Beta strandi116 – 119Combined sources4
Beta strandi130 – 134Combined sources5
Turni135 – 137Combined sources3
Beta strandi139 – 143Combined sources5
Beta strandi150 – 153Combined sources4
Helixi157 – 160Combined sources4
Beta strandi165 – 172Combined sources8
Turni173 – 176Combined sources4
Beta strandi177 – 182Combined sources6
Beta strandi185 – 191Combined sources7
Beta strandi199 – 205Combined sources7
Beta strandi212 – 218Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AFJNMR-A12-224[»]
3EK9X-ray2.60A12-219[»]
ProteinModelPortaliO88838.
SMRiO88838.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88838.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 221B30.2/SPRYPROSITE-ProRule annotationAdd BLAST196
Domaini222 – 264SOCS boxPROSITE-ProRule annotationAdd BLAST43

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.By similarity

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiO88838.
KOiK10344.
OMAiISWPREQ.
OrthoDBiEOG091G0DF0.
PhylomeDBiO88838.
TreeFamiTF312822.

Family and domain databases

InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom_sf.
IPR001496. SOCS_box.
IPR036036. SOCS_box-like_dom_sf.
IPR003877. SPRY_dom.
PfamiView protein in Pfam
PF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
SMARTiView protein in SMART
SM00253. SOCS. 1 hit.
SM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88838-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTALARGS SSTPTSQALY SDFSPPEGLE ELLSAPPPDL VAQRHHGWNP
60 70 80 90 100
KDCSENIDVK EGGLCFERRP VAQSTDGVRG KRGYSRGLHA WEISWPLEQR
110 120 130 140 150
GTHAVVGVAT ALAPLQADHY AALLGSNSES WGWDIGRGKL YHQSKGLEAP
160 170 180 190 200
QYPAGPQGEQ LVVPERLLVV LDMEEGTLGY SIGGTYLGPA FRGLKGRTLY
210 220 230 240 250
PSVSAVWGQC QVRIRYMGER RVEEPQSLLH LSRLCVRHAL GDTRLGQIST
260
LPLPPAMKRY LLYK
Length:264
Mass (Da):28,938
Last modified:November 1, 1998 - v1
Checksum:iE71985597D9B38CB
GO
Isoform 2 (identifier: O88838-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-264: VEEPQSLLHL...PAMKRYLLYK → GEIRTRCGEI...VGFLSLWPVT

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):28,014
Checksum:iE23A2DDAFDFB6773
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018613222 – 264VEEPQ…YLLYK → GEIRTRCGEITTLGNGLGWK LMVGAQEVGFLSLWPVT in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA. Translation: AAC36017.1.
AF403037 mRNA. Translation: AAL57356.1.
AK036451 mRNA. Translation: BAC29435.1.
AK148177 mRNA. Translation: BAE28397.1.
BC002005 mRNA. Translation: AAH02005.1.
BC010305 mRNA. Translation: AAH10305.1.
CCDSiCCDS20529.1. [O88838-1]
RefSeqiNP_001292979.1. NM_001306050.1. [O88838-1]
NP_001292980.1. NM_001306051.1. [O88838-1]
NP_001292981.1. NM_001306052.1. [O88838-1]
NP_001292982.1. NM_001306053.1.
NP_038567.1. NM_013539.2. [O88838-1]
XP_006505630.1. XM_006505567.3. [O88838-1]
UniGeneiMm.261906.

Genome annotation databases

EnsembliENSMUST00000047760; ENSMUSP00000041585; ENSMUSG00000038451. [O88838-1]
ENSMUST00000052727; ENSMUSP00000060124; ENSMUSG00000038451. [O88838-1]
ENSMUST00000112473; ENSMUSP00000108092; ENSMUSG00000038451. [O88838-2]
GeneIDi14794.
KEGGimmu:14794.
UCSCiuc009drw.1. mouse. [O88838-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPSB2_MOUSE
AccessioniPrimary (citable) accession number: O88838
Secondary accession number(s): Q8CBA5, Q91Z15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1998
Last modified: November 22, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families