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Protein

Lactosylceramide alpha-2,3-sialyltransferase

Gene

St3gal5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide).1 Publication

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.9 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Chemistry databases

SwissLipidsiSLP:000000752
SLP:000000759

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide alpha-2,3-sialyltransferase (EC:2.4.99.9)
Alternative name(s):
CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
Ganglioside GM3 synthase
ST3Gal V
Short name:
ST3GalV
Sialyltransferase 9
Gene namesi
Name:St3gal5
Synonyms:GM3S, Siat9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1339963 St3gal5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 65CytoplasmicSequence analysisAdd BLAST65
Transmembranei66 – 86Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini87 – 414LumenalSequence analysisAdd BLAST328

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, and do not show any obvious abnormality apart from an increased sensitivity to insulin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493031 – 414Lactosylceramide alpha-2,3-sialyltransferaseAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi194 ↔ 352By similarity
Glycosylationi235N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO88829
PaxDbiO88829
PeptideAtlasiO88829
PRIDEiO88829

PTM databases

iPTMnetiO88829
PhosphoSitePlusiO88829

Expressioni

Gene expression databases

BgeeiENSMUSG00000056091
ExpressionAtlasiO88829 baseline and differential
GenevisibleiO88829 MM

Interactioni

Protein-protein interaction databases

ELMiO88829
STRINGi10090.ENSMUSP00000070414

Structurei

3D structure databases

ProteinModelPortaliO88829
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00760000119095
HOVERGENiHBG056676
InParanoidiO88829
KOiK03370
OMAiYKYDPPF
OrthoDBiEOG091G098L
PhylomeDBiO88829
TreeFamiTF352819

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88829-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTEAVGGAA RRPQKLRSQA AAPACRAMPS EFTSAKLRSD CSRTSLQWYT
60 70 80 90 100
RTQHKMRRPS LLIKDICKCT LVAFGVWLLY ILILNYTAEE CDMKRMHYVD
110 120 130 140 150
PDRIKRAQSY AQEVLQKECR PRYAKTAMAL LFEDRYSINL EPFVQKVPTA
160 170 180 190 200
SEAELKYDPP FGFRKFSSKV QSLLDMLPEH DFPEHLRAKA CKRCVVVGNG
210 220 230 240 250
GILHGLELGH ALNQFDVVIR LNSAPVEGYS EHVGNKTTIR MTYPEGAPLS
260 270 280 290 300
DVEYYANDLF VTVLFKSVDF KWLQAMVKNE SLPFWVRLFF WKQVAEKVPL
310 320 330 340 350
QPKHFRILNP VIIKETAFDI LQYSEPQSRF WGHDKNIPTI GVIAVVLATH
360 370 380 390 400
LCDEVSLAGF GYDLSQPRTP LHYFDSQCMG AMHWQVMHNV TTETKFLLKL
410
LKEGVVEDLS GGIH
Length:414
Mass (Da):47,360
Last modified:May 20, 2008 - v2
Checksum:i3BC5AEE20D7627DD
GO
Isoform 2 (identifier: O88829-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MHTEAVGGAARRPQKLRSQAAAPACR → MGAPGELRRCGRGAA

Show »
Length:403
Mass (Da):46,127
Checksum:i9B344FEADD62FCA8
GO

Sequence cautioni

The sequence AAF66147 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA33491 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA76467 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB28571 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE34763 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA75235 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA75236 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC79655 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139N → K in BAE34763 (PubMed:16141072).Curated1
Sequence conflicti183P → S in CAA75235 (PubMed:9875239).Curated1
Sequence conflicti183P → S in CAA75236 (PubMed:9875239).Curated1
Sequence conflicti232H → Y in BAE34763 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0336891 – 26MHTEA…APACR → MGAPGELRRCGRGAA in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15003 mRNA Translation: CAA75235.1 Different initiation.
Y15003 mRNA Translation: CAA75236.1 Different initiation.
AB018048 mRNA Translation: BAA33491.1 Different initiation.
AF119416 mRNA Translation: AAF66147.1 Different initiation.
AK012961 mRNA Translation: BAB28571.1 Different initiation.
AK159000 mRNA Translation: BAE34763.1 Different initiation.
AK165726 mRNA Translation: BAE38355.1
BC138557 mRNA Translation: AAI38558.1
BC138559 mRNA Translation: AAI38560.1
AB013302 mRNA Translation: BAA76467.1 Different initiation.
Y18022 Genomic DNA Translation: CAC79654.1
Y18023 Genomic DNA Translation: CAC79655.1 Sequence problems.
CCDSiCCDS20237.1 [O88829-1]
PIRiJE0364
RefSeqiNP_001030305.1, NM_001035228.2 [O88829-1]
NP_035505.2, NM_011375.3
XP_006505880.1, XM_006505817.3
UniGeneiMm.38248

Genome annotation databases

EnsembliENSMUST00000069994; ENSMUSP00000070414; ENSMUSG00000056091 [O88829-1]
GeneIDi20454
KEGGimmu:20454
UCSCiuc009chy.2 mouse [O88829-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSIAT9_MOUSE
AccessioniPrimary (citable) accession number: O88829
Secondary accession number(s): B2RRS1
, Q3TMT6, Q3TY01, Q91YF2, Q91YF3, Q9CZ65, Q9QWF8, Q9QWF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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