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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC2

Gene

Polr2f

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).By similarity

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: RGD

GO - Biological processi

  • transcription from RNA polymerase III promoter Source: RGD
  • transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription from RNA polymerase I promoter Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-RNO-5250924. B-WICH complex positively regulates rRNA expression.
R-RNO-5578749. Transcriptional regulation by small RNAs.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6781823. Formation of TC-NER Pre-Incision Complex.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73762. RNA Polymerase I Transcription Initiation.
R-RNO-73772. RNA Polymerase I Promoter Escape.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73777. RNA Polymerase I Chain Elongation.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-73863. RNA Polymerase I Transcription Termination.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-RNO-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-RNO-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Short name:
RNA polymerases I, II, and III subunit ABC2
Alternative name(s):
DNA-directed RNA polymerase II subunit F
RPB6 homolog
Gene namesi
Name:Polr2f
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi708567. Polr2f.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: RGD
  • DNA-directed RNA polymerase II, core complex Source: UniProtKB
  • DNA-directed RNA polymerase III complex Source: RGD
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 127126DNA-directed RNA polymerases I, II, and III subunit RPABC2PRO_0000133802Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21Phosphoserine; by CK2Combined sources1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO88828.
PRIDEiO88828.

PTM databases

iPTMnetiO88828.
PhosphoSiteiO88828.

Expressioni

Gene expression databases

GenevisibleiO88828. RN.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi249722. 1 interaction.
STRINGi10116.ENSRNOP00000015009.

Structurei

3D structure databases

ProteinModelPortaliO88828.
SMRiO88828. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3405. Eukaryota.
COG1758. LUCA.
GeneTreeiENSGT00390000010415.
HOGENOMiHOG000225272.
HOVERGENiHBG002375.
InParanoidiO88828.
KOiK03014.
OMAiMKYERAR.
OrthoDBiEOG757D0G.
PhylomeDBiO88828.
TreeFamiTF103041.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
InterProiIPR020708. DNA-dir_RNA_polK_14-18kDa_CS.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
IPR028363. RPB6.
IPR006111. RpoK/Rpb6.
[Graphical view]
PANTHERiPTHR10773. PTHR10773. 1 hit.
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
PIRSFiPIRSF500154. RPB6. 1 hit.
PIRSF000778. RpoK/RPB6. 1 hit.
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
PROSITEiPS01111. RNA_POL_K_14KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK
60 70 80 90 100
RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDPL LIAMKELKAR
110 120
KIPIIIRRYL PDGSYEDWGV DELIISD
Length:127
Mass (Da):14,464
Last modified:November 1, 1998 - v1
Checksum:i6366A0D7EB3F0921
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017711 mRNA. Translation: BAA33414.1.
BC083552 mRNA. Translation: AAH83552.1.
RefSeqiNP_112625.1. NM_031335.3.
UniGeneiRn.28212.

Genome annotation databases

EnsembliENSRNOT00000015009; ENSRNOP00000015009; ENSRNOG00000011214.
GeneIDi83503.
KEGGirno:83503.
UCSCiRGD:708567. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017711 mRNA. Translation: BAA33414.1.
BC083552 mRNA. Translation: AAH83552.1.
RefSeqiNP_112625.1. NM_031335.3.
UniGeneiRn.28212.

3D structure databases

ProteinModelPortaliO88828.
SMRiO88828. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249722. 1 interaction.
STRINGi10116.ENSRNOP00000015009.

PTM databases

iPTMnetiO88828.
PhosphoSiteiO88828.

Proteomic databases

PaxDbiO88828.
PRIDEiO88828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015009; ENSRNOP00000015009; ENSRNOG00000011214.
GeneIDi83503.
KEGGirno:83503.
UCSCiRGD:708567. rat.

Organism-specific databases

CTDi5435.
RGDi708567. Polr2f.

Phylogenomic databases

eggNOGiKOG3405. Eukaryota.
COG1758. LUCA.
GeneTreeiENSGT00390000010415.
HOGENOMiHOG000225272.
HOVERGENiHBG002375.
InParanoidiO88828.
KOiK03014.
OMAiMKYERAR.
OrthoDBiEOG757D0G.
PhylomeDBiO88828.
TreeFamiTF103041.

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-RNO-5250924. B-WICH complex positively regulates rRNA expression.
R-RNO-5578749. Transcriptional regulation by small RNAs.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6781823. Formation of TC-NER Pre-Incision Complex.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73762. RNA Polymerase I Transcription Initiation.
R-RNO-73772. RNA Polymerase I Promoter Escape.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73777. RNA Polymerase I Chain Elongation.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-73863. RNA Polymerase I Transcription Termination.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-RNO-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-RNO-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiO88828.

Gene expression databases

GenevisibleiO88828. RN.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
InterProiIPR020708. DNA-dir_RNA_polK_14-18kDa_CS.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
IPR028363. RPB6.
IPR006111. RpoK/Rpb6.
[Graphical view]
PANTHERiPTHR10773. PTHR10773. 1 hit.
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
PIRSFiPIRSF500154. RPB6. 1 hit.
PIRSF000778. RpoK/RPB6. 1 hit.
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
PROSITEiPS01111. RNA_POL_K_14KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A serine residue in the N-terminal acidic region of rat RPB6, one of the common subunits of RNA polymerases, is exclusively phosphorylated by casein kinase II in vitro."
    Kayukawa K., Makino Y., Yogosawa S., Tamura T.
    Gene 234:139-147(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-2.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPAB2_RAT
AccessioniPrimary (citable) accession number: O88828
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.