Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lathosterol oxidase

Gene

Sc5d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes a dehydrogenation to introduce C5-6 double bond into lathosterol.

Catalytic activityi

A Delta(7)-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a Delta(5,7)-sterol + 2 ferricytochrome b5 + 2 H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, NAD, NADP

Enzyme and pathway databases

BRENDAi1.14.21.6. 3474.
ReactomeiR-MMU-2426168. Activation of gene expression by SREBF (SREBP).
R-MMU-6807047. Cholesterol biosynthesis via desmosterol.
R-MMU-6807062. Cholesterol biosynthesis via lathosterol.

Names & Taxonomyi

Protein namesi
Recommended name:
Lathosterol oxidase (EC:1.14.19.20)
Alternative name(s):
C-5 sterol desaturase
Delta(7)-sterol 5-desaturase
Delta(7)-sterol C5(6)-desaturase
Lathosterol 5-desaturase
Sterol-C5-desaturase
Gene namesi
Name:Sc5d
Synonyms:Sc5dl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353611. Sc5d.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170291 – 299Lathosterol oxidaseAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88822.
PaxDbiO88822.
PeptideAtlasiO88822.
PRIDEiO88822.

PTM databases

iPTMnetiO88822.
PhosphoSitePlusiO88822.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032018.
GenevisibleiO88822. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057354.

Structurei

3D structure databases

ProteinModelPortaliO88822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 143Histidine box-16
Motifi151 – 155Histidine box-25
Motifi228 – 233Histidine box-36

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0872. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00550000075101.
HOGENOMiHOG000200579.
HOVERGENiHBG012628.
InParanoidiO88822.
KOiK00227.
OMAiGDFRVPH.
OrthoDBiEOG091G0C85.
TreeFamiTF300797.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVLSAADY YFFTPYVYPA TWPEDNIIRQ TISLLIVTNL GAYILYFFCA
60 70 80 90 100
TLSYYFVYDH SLMKHPQFLK NQVSREIVFT VKSLPWISIP TVSLFLLELR
110 120 130 140 150
GYSKLYDDIG DFPNGWIHLM VSVVSFLFFT DMLIYWIHRG LHHRLVYKRI
160 170 180 190 200
HKPHHIWKIP TPFASHAFHP VDGFLQSLPY HIYPFVFPLH KVVYLGLYVL
210 220 230 240 250
VNVWTISIHD GDFRVPQILR PFINGSAHHT DHHMFFDYNY GQYFTLWDRI
260 270 280 290
GGSFKHPSSF EGKGPHSYVK NMTEKESNSF AENGCKGKKV GNGEFTKNK
Length:299
Mass (Da):35,062
Last modified:September 18, 2013 - v2
Checksum:i96F3AFF61F7ED707
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136W → R in BAA33730 (PubMed:10786622).Curated1
Sequence conflicti291G → S in BAA33730 (PubMed:10786622).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016248 mRNA. Translation: BAA33730.1.
AK043825 mRNA. Translation: BAC31666.1.
AK077670 mRNA. Translation: BAC36944.1.
AC160051 Genomic DNA. No translation available.
CCDSiCCDS23086.1.
RefSeqiNP_766357.1. NM_172769.2.
XP_006510316.1. XM_006510253.2.
UniGeneiMm.32700.
Mm.392107.
Mm.470142.

Genome annotation databases

EnsembliENSMUST00000052725; ENSMUSP00000057354; ENSMUSG00000032018.
ENSMUST00000169609; ENSMUSP00000130438; ENSMUSG00000032018.
GeneIDi235293.
KEGGimmu:235293.
UCSCiuc009par.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016248 mRNA. Translation: BAA33730.1.
AK043825 mRNA. Translation: BAC31666.1.
AK077670 mRNA. Translation: BAC36944.1.
AC160051 Genomic DNA. No translation available.
CCDSiCCDS23086.1.
RefSeqiNP_766357.1. NM_172769.2.
XP_006510316.1. XM_006510253.2.
UniGeneiMm.32700.
Mm.392107.
Mm.470142.

3D structure databases

ProteinModelPortaliO88822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057354.

PTM databases

iPTMnetiO88822.
PhosphoSitePlusiO88822.

Proteomic databases

EPDiO88822.
PaxDbiO88822.
PeptideAtlasiO88822.
PRIDEiO88822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052725; ENSMUSP00000057354; ENSMUSG00000032018.
ENSMUST00000169609; ENSMUSP00000130438; ENSMUSG00000032018.
GeneIDi235293.
KEGGimmu:235293.
UCSCiuc009par.1. mouse.

Organism-specific databases

CTDi6309.
MGIiMGI:1353611. Sc5d.

Phylogenomic databases

eggNOGiKOG0872. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00550000075101.
HOGENOMiHOG000200579.
HOVERGENiHBG012628.
InParanoidiO88822.
KOiK00227.
OMAiGDFRVPH.
OrthoDBiEOG091G0C85.
TreeFamiTF300797.

Enzyme and pathway databases

BRENDAi1.14.21.6. 3474.
ReactomeiR-MMU-2426168. Activation of gene expression by SREBF (SREBP).
R-MMU-6807047. Cholesterol biosynthesis via desmosterol.
R-MMU-6807062. Cholesterol biosynthesis via lathosterol.

Miscellaneous databases

PROiO88822.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032018.
GenevisibleiO88822. MM.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC5D_MOUSE
AccessioniPrimary (citable) accession number: O88822
Secondary accession number(s): Q8BGI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 18, 2013
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.