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O88822 (SC5D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lathosterol oxidase

EC=1.14.21.6
Alternative name(s):
C-5 sterol desaturase
Delta(7)-sterol 5-desaturase
Lathosterol 5-desaturase
Sterol-C5-desaturase
Gene names
Name:Sc5d
Synonyms:Sc5dl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes a dehydrogenation to introduce C5-6 double bond into lathosterol.

Catalytic activity

5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = cholesta-5,7-dien-3-beta-ol + NAD(P)+ + 2 H2O.

Cofactor

Iron By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similarities

Belongs to the sterol desaturase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Lathosterol oxidase
PRO_0000117029

Regions

Transmembrane32 – 5221Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane186 – 20621Helical; Potential
Motif138 – 1436Histidine box-1
Motif151 – 1555Histidine box-2
Motif228 – 2336Histidine box-3

Amino acid modifications

Modified residue2531Phosphoserine By similarity

Experimental info

Sequence conflict1361W → R in BAA33730. Ref.1
Sequence conflict2911G → S in BAA33730. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O88822 [UniParc].

Last modified September 18, 2013. Version 2.
Checksum: 96F3AFF61F7ED707

FASTA29935,062
        10         20         30         40         50         60 
MDLVLSAADY YFFTPYVYPA TWPEDNIIRQ TISLLIVTNL GAYILYFFCA TLSYYFVYDH 

        70         80         90        100        110        120 
SLMKHPQFLK NQVSREIVFT VKSLPWISIP TVSLFLLELR GYSKLYDDIG DFPNGWIHLM 

       130        140        150        160        170        180 
VSVVSFLFFT DMLIYWIHRG LHHRLVYKRI HKPHHIWKIP TPFASHAFHP VDGFLQSLPY 

       190        200        210        220        230        240 
HIYPFVFPLH KVVYLGLYVL VNVWTISIHD GDFRVPQILR PFINGSAHHT DHHMFFDYNY 

       250        260        270        280        290 
GQYFTLWDRI GGSFKHPSSF EGKGPHSYVK NMTEKESNSF AENGCKGKKV GNGEFTKNK 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of the mammalian sterol C5-desaturase and the expression in yeast mutant."
Nishi S., Nishino H., Ishibashi T.
Biochim. Biophys. Acta 1490:106-108(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain cortex.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016248 mRNA. Translation: BAA33730.1.
AK043825 mRNA. Translation: BAC31666.1.
AK077670 mRNA. Translation: BAC36944.1.
AC160051 Genomic DNA. No translation available.
CCDSCCDS23086.1.
RefSeqNP_766357.1. NM_172769.2.
XP_006510316.1. XM_006510253.1.
UniGeneMm.32700.
Mm.392107.
Mm.470142.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000057354.

PTM databases

PhosphoSiteO88822.

Proteomic databases

PRIDEO88822.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052725; ENSMUSP00000057354; ENSMUSG00000032018.
ENSMUST00000169609; ENSMUSP00000130438; ENSMUSG00000032018.
GeneID235293.
KEGGmmu:235293.
UCSCuc009par.1. mouse.

Organism-specific databases

CTD6309.
MGIMGI:1353611. Sc5d.

Phylogenomic databases

eggNOGCOG3000.
GeneTreeENSGT00550000075101.
HOGENOMHOG000200579.
HOVERGENHBG012628.
InParanoidO88822.
KOK00227.
OMAFFCATLS.
OrthoDBEOG7NSB2T.
TreeFamTF300797.

Enzyme and pathway databases

BRENDA1.14.21.6. 3474.

Gene expression databases

GenevestigatorO88822.

Family and domain databases

InterProIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio382567.
PROO88822.
SOURCESearch...

Entry information

Entry nameSC5D_MOUSE
AccessionPrimary (citable) accession number: O88822
Secondary accession number(s): Q8BGI0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 18, 2013
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot