Reviewed, UniProtKB/Swiss-Prot O88816 (SNAT_MOUSE)

Names and origin

Protein names

Recommended name:
    Serotonin N-acetyltransferase
      Short name=Serotonin acetylase
    EC=2.3.1.87
Alternative name(s):
    Aralkylamine N-acetyltransferase
      Short name=AA-NAT

Gene names

Name:	Aanat
Synonyms:	Snat

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the N-acetylation of serotonin into N-acetylserotonin.
Catalytic activity	Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.
Pathway	Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
Subunit structure	Monomer By similarity. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-29, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-203 site, the other YWHAZ monomer with similar effect By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in pineal gland and retina.
Post-translational modification	Phosphorylated; cAMP-dependent phosphorylation on both N-terminal and C-terminal sites regulates AANAT activity through allowing interaction with 14-3-3 proteins and protecting the enzyme against proteasomal degradation By similarity.
Sequence similarities	Belongs to the acetyltransferase family. AANAT subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Biological rhythms Melatonin biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	melatonin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aralkylamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC arylamine N-acetyltransferase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Serotonin N-acetyltransferase		PRO_0000074583
Regions
	Domain	33 – 194	162	N-acetyltransferase
	Region	122 – 124	3	Acetyl-CoA binding By similarity
	Region	130 – 135	6	Acetyl-CoA binding By similarity
	Region	166 – 168	3	Acetyl-CoA binding By similarity
Sites
	Binding site	122	1	Substrate; via carbonyl oxygen By similarity
	Site	118	1	Important for the catalytic mechanism; involved in substrate deprotonation By similarity
	Site	120	1	Important for the catalytic mechanism; involved in substrate deprotonation By similarity
Amino acid modifications
	Modified residue	29	1	Phosphothreonine; by PKA By similarity
	Modified residue	203	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88816-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D2ECD070998CB643	FASTA	205	23,069
	10 20 30 40 50 60 MLNINSLKPE ALHLPLGTSE FLGCQRRHTL PASEFRCLTP EDATSAFEIE REAFISVSGT 70 80 90 100 110 120 CPLYLDEIRH FLTLCPELSL GWFEEGCLVA FIIGSLWDKE RLTQESLTLH RPGGRTAHLH 130 140 150 160 170 180 VLAVHRTFRQ QGKGSVLLWR YLHHLGSQPA VRRAVLMCED ALVPFYEKFG FQAVGPCAIT 190 200 VGSLTFTELQ CSLRCHAFLR RNSGC

References

[1]	"Molecular cloning of serotonin N-acetyltransferase gene from the mouse and its daily expression in the retina." Sakamoto K., Ishida N. Neurosci. Lett. 250:181-184(1998) [PubMed: 9708862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Retina.
[2]	"Natural melatonin 'knockdown' in C57BL/6J mice: rare mechanism truncates serotonin N-acetyltransferase." Roseboom P.H., Namboodiri M.A.A., Zimonjic D.B., Popescu N.C., Rodriguez I.R., Gastel J.A., Klein D.C. Brain Res. Mol. Brain Res. 63:189-197(1998) [PubMed: 9838107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv and C3H/He. Tissue: Pineal gland.
[3]	Roseboom P.H., Namboodiri M.A.A., Zimonjic D.B., Popescu N.C., Rodriguez I.R., Klein D.C. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB013358 mRNA. Translation: BAA31526.1. AF004108 mRNA. Translation: AAD09408.1. U83462 Genomic DNA. Translation: AAD08637.1.
IPI	IPI00135086.
RefSeq	NP_033721.1.
UniGene	Mm.418559 Mm.42233
3D structure databases
HSSP	HSSP built from PDB template 1CJW based on UniProtKB Q29495.
SMR	O88816. Positions 23-194.
ModBase	Search...
Protein-protein interaction databases
STRING	O88816.
PTM databases
PhosphoSite	O88816.
Proteomic databases
PRIDE	O88816.
Genome annotation databases
Ensembl	ENSMUST00000021160; ENSMUSP00000021160; ENSMUSG00000020804; Mus musculus. [Genome view]
GeneID	11298.
KEGG	mmu:11298.
UCSC	uc007mlo.1. mouse.
Organism-specific databases
CTD	11298.
MGI	MGI:1328365. Aanat.
Phylogenomic databases
HOGENOM	O88816.
HOVERGEN	O88816.
OMA	LRRNSGC.
Enzyme and pathway databases
BRENDA	2.3.1.87. 244.
Gene expression databases
ArrayExpress	O88816.
Bgee	O88816.
CleanEx	MM_AANAT.
Genevestigator	O88816.
GermOnline	ENSMUSG00000020804. Mus musculus.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	278578.
SOURCE	Search...

Entry information

Entry name

SNAT_MOUSE

Accession

Primary (citable) accession number: O88816

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)