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O88816

- SNAT_MOUSE

UniProt

O88816 - SNAT_MOUSE

Protein

Serotonin N-acetyltransferase

Gene

Aanat

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

    Catalytic activityi

    Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei118 – 1181Important for the catalytic mechanism; involved in substrate deprotonationBy similarity
    Sitei120 – 1201Important for the catalytic mechanism; involved in substrate deprotonationBy similarity
    Binding sitei122 – 1221Substrate; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. aralkylamine N-acetyltransferase activity Source: UniProtKB
    2. arylamine N-acetyltransferase activity Source: MGI

    GO - Biological processi

    1. cellular response to cAMP Source: UniProtKB
    2. circadian rhythm Source: UniProtKB
    3. melatonin biosynthetic process Source: UniProtKB
    4. N-terminal protein amino acid acetylation Source: UniProtKB
    5. response to calcium ion Source: Ensembl
    6. response to copper ion Source: Ensembl
    7. response to corticosterone Source: Ensembl
    8. response to cytokine Source: Ensembl
    9. response to insulin Source: Ensembl
    10. response to light stimulus Source: Ensembl
    11. response to prostaglandin E Source: Ensembl
    12. response to zinc ion Source: Ensembl

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Biological rhythms, Melatonin biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_218437. Serotonin and melatonin biosynthesis.
    UniPathwayiUPA00837; UER00815.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serotonin N-acetyltransferase (EC:2.3.1.87)
    Short name:
    Serotonin acetylase
    Alternative name(s):
    Aralkylamine N-acetyltransferase
    Short name:
    AA-NAT
    Gene namesi
    Name:Aanat
    Synonyms:Snat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1328365. Aanat.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. perinuclear region of cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Serotonin N-acetyltransferasePRO_0000074583Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Phosphothreonine; by PKABy similarity
    Modified residuei203 – 2031PhosphoserineBy similarity

    Post-translational modificationi

    cAMP-dependent phosphorylation on both N-terminal Thr-29 and C-terminal Ser-203 regulates AANAT activity by promoting interaction with 14-3-3 proteins.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO88816.

    PTM databases

    PhosphoSiteiO88816.

    Expressioni

    Tissue specificityi

    Highly expressed in pineal gland at night. Expression in the retina has not been confirmed. Extrapineal expression could be strain-specific.1 Publication

    Inductioni

    Exhibits night/day variations with a drastically increased expression at night in the pineal gland.3 Publications

    Gene expression databases

    ArrayExpressiO88816.
    BgeeiO88816.
    CleanExiMM_AANAT.
    GenevestigatoriO88816.

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with several 14-3-3 proteins, including YWHAB, YWHAE, YWHAG and YWHAZ, preferentially when phosphorylated at Thr-29 By similarity. Phosphorylation on Ser-203 also allows binding to YWHAZ, but with lower affinity By similarity. The interaction with YWHAZ considerably increases affinity for arylalkylamines and acetyl-CoA and protects the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO88816.
    SMRiO88816. Positions 28-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 194162N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni122 – 1243Acetyl-CoA bindingBy similarity
    Regioni130 – 1356Acetyl-CoA bindingBy similarity
    Regioni166 – 1683Acetyl-CoA bindingBy similarity

    Sequence similaritiesi

    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0454.
    HOVERGENiHBG016332.
    InParanoidiO88816.
    KOiK00669.
    OMAiLRRNSGC.
    PhylomeDBiO88816.
    TreeFamiTF331622.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88816-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNINSLKPE ALHLPLGTSE FLGCQRRHTL PASEFRCLTP EDATSAFEIE    50
    REAFISVSGT CPLYLDEIRH FLTLCPELSL GWFEEGCLVA FIIGSLWDKE 100
    RLTQESLTLH RPGGRTAHLH VLAVHRTFRQ QGKGSVLLWR YLHHLGSQPA 150
    VRRAVLMCED ALVPFYEKFG FQAVGPCAIT VGSLTFTELQ CSLRCHAFLR 200
    RNSGC 205
    Length:205
    Mass (Da):23,069
    Last modified:November 1, 1998 - v1
    Checksum:iD2ECD070998CB643
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013358 mRNA. Translation: BAA31526.1.
    AF004108 mRNA. Translation: AAD09408.1.
    U83462 Genomic DNA. Translation: AAD08637.1.
    CCDSiCCDS25671.1.
    RefSeqiNP_033721.1. NM_009591.3.
    UniGeneiMm.418559.
    Mm.42233.

    Genome annotation databases

    EnsembliENSMUST00000153476; ENSMUSP00000122895; ENSMUSG00000020804.
    GeneIDi11298.
    KEGGimmu:11298.
    UCSCiuc007mlo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013358 mRNA. Translation: BAA31526.1 .
    AF004108 mRNA. Translation: AAD09408.1 .
    U83462 Genomic DNA. Translation: AAD08637.1 .
    CCDSi CCDS25671.1.
    RefSeqi NP_033721.1. NM_009591.3.
    UniGenei Mm.418559.
    Mm.42233.

    3D structure databases

    ProteinModelPortali O88816.
    SMRi O88816. Positions 28-193.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O88816.

    Proteomic databases

    PRIDEi O88816.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000153476 ; ENSMUSP00000122895 ; ENSMUSG00000020804 .
    GeneIDi 11298.
    KEGGi mmu:11298.
    UCSCi uc007mlo.2. mouse.

    Organism-specific databases

    CTDi 15.
    MGIi MGI:1328365. Aanat.

    Phylogenomic databases

    eggNOGi COG0454.
    HOVERGENi HBG016332.
    InParanoidi O88816.
    KOi K00669.
    OMAi LRRNSGC.
    PhylomeDBi O88816.
    TreeFami TF331622.

    Enzyme and pathway databases

    UniPathwayi UPA00837 ; UER00815 .
    Reactomei REACT_218437. Serotonin and melatonin biosynthesis.

    Miscellaneous databases

    NextBioi 278578.
    PROi O88816.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88816.
    Bgeei O88816.
    CleanExi MM_AANAT.
    Genevestigatori O88816.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view ]
    Pfami PF00583. Acetyltransf_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of serotonin N-acetyltransferase gene from the mouse and its daily expression in the retina."
      Sakamoto K., Ishida N.
      Neurosci. Lett. 250:181-184(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Strain: BALB/c.
      Tissue: Retina.
    2. "Natural melatonin 'knockdown' in C57BL/6J mice: rare mechanism truncates serotonin N-acetyltransferase."
      Roseboom P.H., Namboodiri M.A.A., Zimonjic D.B., Popescu N.C., Rodriguez I.R., Gastel J.A., Klein D.C.
      Brain Res. Mol. Brain Res. 63:189-197(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, INDUCTION, IDENTIFICATION OF A TRUNCATED FORM IN C57BL/6.
      Strain: 129/Sv and C3H/He.
      Tissue: Pineal gland.
    3. "Genetic variation of melatonin productivity in laboratory mice under domestication."
      Kasahara T., Abe K., Mekada K., Yoshiki A., Kato T.
      Proc. Natl. Acad. Sci. U.S.A. 107:6412-6417(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: C3H/He and C57BL/6J.
      Tissue: Pineal gland.

    Entry informationi

    Entry nameiSNAT_MOUSE
    AccessioniPrimary (citable) accession number: O88816
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Pineal melatonin synthesis is severely compromised in most inbred strains of mice. In C57BL/6, a polymorphism activates a cryptic splice site causing the production of an alternative form containing a premature stop codon. The predicted resulting protein would lack the putative catalytic and acetyl-CoA binding domains and therefore would be inactive.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3