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Protein

Signal transducing adapter molecule 2

Gene

Stam2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).By similarity1 Publication

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-182971 EGFR downregulation
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6807004 Negative regulation of MET activity
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 2
Short name:
STAM-2
Alternative name(s):
Hrs-binding protein
Gene namesi
Name:Stam2
Synonyms:Hbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1929100 Stam2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Adult mice lacking Stam and Stam2 due to inducible gene targeting exhibit significant reduction in T-cell development in the thymus and profound reduction in the peripheral mature T-cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176L → A: Loss of interaction with UBQLN1; when associated with A-177. 1 Publication1
Mutagenesisi177S → A: Loss of interaction with UBQLN1; when associated with A-176. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901481 – 523Signal transducing adapter molecule 2Add BLAST523

Post-translational modificationi

Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88811
PaxDbiO88811
PeptideAtlasiO88811
PRIDEiO88811

PTM databases

iPTMnetiO88811
PhosphoSitePlusiO88811

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000055371
ExpressionAtlasiO88811 baseline and differential
GenevisibleiO88811 MM

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY. Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct (By similarity). Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain).By similarity1 Publication

Protein-protein interaction databases

BioGridi207904, 7 interactors
CORUMiO88811
IntActiO88811, 5 interactors
MINTiO88811
STRINGi10090.ENSMUSP00000099820

Structurei

Secondary structure

1523
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi205 – 211Combined sources7
Beta strandi228 – 233Combined sources6
Beta strandi236 – 244Combined sources9
Beta strandi247 – 252Combined sources6
Helixi253 – 255Combined sources3
Beta strandi256 – 258Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UJ0X-ray1.70A204-261[»]
ProteinModelPortaliO88811
SMRiO88811
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88811

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 144VHSPROSITE-ProRule annotationAdd BLAST129
Domaini165 – 184UIMPROSITE-ProRule annotationAdd BLAST20
Domaini202 – 261SH3PROSITE-ProRule annotationAdd BLAST60
Domaini360 – 377ITAMAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 220Interaction with USP81 Publication2
Regioni334 – 368Interaction with HGSAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi54 – 67PxVxL motifAdd BLAST14

Domaini

The VHS and UIM domains mediate the interaction with ubiquitinated proteins.1 Publication
The SH3 domain mediates the interaction with USP8.1 Publication
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.1 Publication

Sequence similaritiesi

Belongs to the STAM family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199 Eukaryota
ENOG410XTSY LUCA
GeneTreeiENSGT00870000136415
HOGENOMiHOG000231952
HOVERGENiHBG053175
InParanoidiO88811
KOiK04705
OMAiHGGNYMG
OrthoDBiEOG091G0H1Y
PhylomeDBiO88811
TreeFamiTF315007

Family and domain databases

CDDicd11963 SH3_STAM2, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR008942 ENTH_VHS
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035675 STAM2_SH3
IPR003903 UIM_dom
IPR002014 VHS_dom
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PF02809 UIM, 1 hit
PF00790 VHS, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SM00726 UIM, 1 hit
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50330 UIM, 1 hit
PS50179 VHS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88811-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLFTANPFE QDVEKATNEY NTTEDWSLIM DICDRVGSTP SGAKDCLKAI
60 70 80 90 100
MKRVNHKVPH VALQALTLLG ACVANCGKIF HLEVCSRDFA TEVRSVIKNK
110 120 130 140 150
AHPKVCEKLK SLMVEWSEEF QKDPQFSLIS ATIKSMKEEG VTFPSAGSQT
160 170 180 190 200
VAAAAKNGTS LNKNKEDEDI AKAIELSLQE QKQQYTETKA LYPPAESQLN
210 220 230 240 250
NKAARRVRAL YDFEAVEDNE LTFKHGELIT VLDDSDANWW QGENHRGTGL
260 270 280 290 300
FPSNFVTTDL STEVETATVD KLNVIDDDVE EIKKSEPEPV YIDEGKMDRA
310 320 330 340 350
LQILQSIDPK ESKPDSQDLL DLEDVCQQMG PMIDEKLEEI DRKHSELSEL
360 370 380 390 400
NVKVLEALDL YNKLVNEAPV YSVYSKLHPA HYPPAAAGVP VQTYPVQSHG
410 420 430 440 450
GNYLGHGIHQ VSVAQNYNLG PDPMGSLRSL PPNMNSVTAH TVQPPYLSTG
460 470 480 490 500
QDTVSNPSYM NQSSRLQAAA GTAAYTQPVG MSTDVSSFQN TASGLPQLAG
510 520
FPVAVPAPVA AQPQASYHQQ PLL
Length:523
Mass (Da):57,455
Last modified:November 1, 1998 - v1
Checksum:iCE00B59F10BA0161
GO
Isoform 2 (identifier: O88811-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-100: Missing.
     448-448: S → R
     449-523: Missing.

Show »
Length:415
Mass (Da):46,260
Checksum:i305B94A59C0CA08B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01484968 – 100Missing in isoform 2. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_014850448S → R in isoform 2. 1 Publication1
Alternative sequenceiVSP_014851449 – 523Missing in isoform 2. 1 PublicationAdd BLAST75

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012611 mRNA Translation: BAA33547.1
AK004604 mRNA Translation: BAB23403.1
AK044230 mRNA Translation: BAC31830.1
AL928960 Genomic DNA No translation available.
BC013818 mRNA Translation: AAH13818.1
CCDSiCCDS16037.1 [O88811-1]
RefSeqiNP_062641.1, NM_019667.2 [O88811-1]
UniGeneiMm.263639

Genome annotation databases

EnsembliENSMUST00000102759; ENSMUSP00000099820; ENSMUSG00000055371 [O88811-1]
ENSMUST00000127316; ENSMUSP00000121898; ENSMUSG00000055371 [O88811-2]
GeneIDi56324
KEGGimmu:56324
UCSCiuc008jre.1 mouse [O88811-1]
uc008jrf.1 mouse [O88811-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSTAM2_MOUSE
AccessioniPrimary (citable) accession number: O88811
Secondary accession number(s): A2AU00, Q8C8Y4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health