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O88811 (STAM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducing adapter molecule 2

Short name=STAM-2
Alternative name(s):
Hrs-binding protein
Gene names
Name:Stam2
Synonyms:Hbp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes By similarity. Ref.8

Subunit structure

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY. Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct By similarity. Ref.1 Ref.5 Ref.7 Ref.8

Subcellular location

Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity Ref.1 Ref.7.

Tissue specificity

Ubiquitously expressed, with highest levels in testis. Ref.1

Domain

The VHS and UIM domains mediate the interaction with ubiquitinated proteins. Ref.1

The SH3 domain mediates the interaction with USP8. Ref.1

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. Ref.1

Post-translational modification

Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF By similarity.

Disruption phenotype

Adult mice lacking Stam1 and Stam2 due to inducible gene targeting exhibit significant reduction in T-cell development in the thymus and profound reduction in the peripheral mature T-cells. Ref.6

Sequence similarities

Belongs to the STAM family.

Contains 1 ITAM domain.

Contains 1 SH3 domain.

Contains 1 UIM (ubiquitin-interacting motif) repeat.

Contains 1 VHS domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Endosome
Membrane
   Coding sequence diversityAlternative splicing
   DomainSH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular protein transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentearly endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein binding

Inferred from physical interaction Ref.5. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O88811-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O88811-2)

The sequence of this isoform differs from the canonical sequence as follows:
     68-100: Missing.
     448-448: S → R
     449-523: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Signal transducing adapter molecule 2
PRO_0000190148

Regions

Domain16 – 144129VHS
Repeat165 – 18420UIM
Domain202 – 26160SH3
Domain360 – 37718ITAM
Region219 – 2202Interaction with USP8
Region334 – 36835Interaction with HGS
Motif54 – 6714PxVxL motif

Natural variations

Alternative sequence68 – 10033Missing in isoform 2.
VSP_014849
Alternative sequence4481S → R in isoform 2.
VSP_014850
Alternative sequence449 – 52375Missing in isoform 2.
VSP_014851

Secondary structure

........... 523
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: CE00B59F10BA0161

FASTA52357,455
        10         20         30         40         50         60 
MPLFTANPFE QDVEKATNEY NTTEDWSLIM DICDRVGSTP SGAKDCLKAI MKRVNHKVPH 

        70         80         90        100        110        120 
VALQALTLLG ACVANCGKIF HLEVCSRDFA TEVRSVIKNK AHPKVCEKLK SLMVEWSEEF 

       130        140        150        160        170        180 
QKDPQFSLIS ATIKSMKEEG VTFPSAGSQT VAAAAKNGTS LNKNKEDEDI AKAIELSLQE 

       190        200        210        220        230        240 
QKQQYTETKA LYPPAESQLN NKAARRVRAL YDFEAVEDNE LTFKHGELIT VLDDSDANWW 

       250        260        270        280        290        300 
QGENHRGTGL FPSNFVTTDL STEVETATVD KLNVIDDDVE EIKKSEPEPV YIDEGKMDRA 

       310        320        330        340        350        360 
LQILQSIDPK ESKPDSQDLL DLEDVCQQMG PMIDEKLEEI DRKHSELSEL NVKVLEALDL 

       370        380        390        400        410        420 
YNKLVNEAPV YSVYSKLHPA HYPPAAAGVP VQTYPVQSHG GNYLGHGIHQ VSVAQNYNLG 

       430        440        450        460        470        480 
PDPMGSLRSL PPNMNSVTAH TVQPPYLSTG QDTVSNPSYM NQSSRLQAAA GTAAYTQPVG 

       490        500        510        520 
MSTDVSSFQN TASGLPQLAG FPVAVPAPVA AQPQASYHQQ PLL 

« Hide

Isoform 2 [UniParc].

Checksum: 305B94A59C0CA08B
Show »

FASTA41546,260

References

« Hide 'large scale' references
[1]"A hrs binding protein having a Src homology 3 domain is involved in intracellular degradation of growth factors and their receptors."
Takata H., Kato M., Denda K., Kitamura N.
Genes Cells 5:57-69(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH HGS, DOMAIN, SUBCELLULAR LOCATION.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Lung.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"A deubiquitinating enzyme UBPY interacts with the Src homology 3 domain of Hrs-binding protein via a novel binding motif PX(V/I)(D/N)RXXKP."
Kato M., Miyazawa K., Kitamura N.
J. Biol. Chem. 275:37481-37487(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH USP8.
[6]"Signal-transducing adaptor molecules STAM1 and STAM2 are required for T-cell development and survival."
Yamada M., Ishii N., Asao H., Murata K., Kanazawa C., Sasaki H., Sugamura K.
Mol. Cell. Biol. 22:8648-8658(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes."
Bache K.G., Raiborg C., Mehlum A., Stenmark H.
J. Biol. Chem. 278:12513-12521(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HGS; EPS15 AND UBIQUITIN, SUBCELLULAR LOCATION.
[8]"STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif."
Mizuno E., Kawahata K., Kato M., Kitamura N., Komada M.
Mol. Biol. Cell 14:3675-3689(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBIQUITIN, FUNCTION.
[9]"Structural insight into modest binding of a non-PXXP ligand to the signal transducing adaptor molecule-2 Src homology 3 domain."
Kaneko T., Kumasaka T., Ganbe T., Sato T., Miyazawa K., Kitamura N., Tanaka N.
J. Biol. Chem. 278:48162-48168(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 203-260 IN COMPLEX WITH USP8.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012611 mRNA. Translation: BAA33547.1.
AK004604 mRNA. Translation: BAB23403.1.
AK044230 mRNA. Translation: BAC31830.1.
AL928960 Genomic DNA. Translation: CAM23666.1.
BC013818 mRNA. Translation: AAH13818.1.
CCDSCCDS16037.1. [O88811-1]
RefSeqNP_062641.1. NM_019667.2. [O88811-1]
UniGeneMm.263639.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UJ0X-ray1.70A204-261[»]
ProteinModelPortalO88811.
SMRO88811. Positions 1-150, 204-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207904. 8 interactions.
IntActO88811. 5 interactions.
MINTMINT-144894.

PTM databases

PhosphoSiteO88811.

Proteomic databases

MaxQBO88811.
PaxDbO88811.
PRIDEO88811.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102759; ENSMUSP00000099820; ENSMUSG00000055371. [O88811-1]
ENSMUST00000127316; ENSMUSP00000121898; ENSMUSG00000055371. [O88811-2]
GeneID56324.
KEGGmmu:56324.
UCSCuc008jre.1. mouse. [O88811-1]
uc008jrf.1. mouse. [O88811-2]

Organism-specific databases

CTD10254.
MGIMGI:1929100. Stam2.

Phylogenomic databases

eggNOGNOG309509.
GeneTreeENSGT00700000104139.
HOGENOMHOG000231952.
HOVERGENHBG053175.
InParanoidO88811.
KOK04705.
OMAHYPPASS.
OrthoDBEOG7353WJ.
PhylomeDBO88811.
TreeFamTF315007.

Gene expression databases

ArrayExpressO88811.
BgeeO88811.
GenevestigatorO88811.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
InterProIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. Ubiquitin-int_motif.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSTAM2. mouse.
EvolutionaryTraceO88811.
NextBio312298.
PROO88811.
SOURCESearch...

Entry information

Entry nameSTAM2_MOUSE
AccessionPrimary (citable) accession number: O88811
Secondary accession number(s): A2AU00, Q8C8Y4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot