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O88809 (DCX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal migration protein doublecortin
Alternative name(s):
Doublin
Lissencephalin-X
Short name=Lis-X
Gene names
Name:Dcx
Synonyms:Dcn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration By similarity.

Subcellular location

Cytoplasm Potential. Cell projection By similarity. Note: Localizes at neurite tips By similarity.

Tissue specificity

In neonatal tissues, highly expressed in brain, but not expressed in heart, liver, kidney and spleen. In adult tissues, faintly expressed in brain but not expressed in muscle, heart, lung, liver, spleen, intestine, kidney, testis and placenta.

Developmental stage

Already expressed by embryonic day 11 (E11), maximally around birth, expression decreasing gradually during the second postnatal week, with no expression in adult stages.

Post-translational modification

Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules By similarity.

Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21.

Sequence similarities

Contains 2 doublecortin domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Neuronal migration protein doublecortin
PRO_0000079834

Regions

Domain53 – 13987Doublecortin 1
Domain180 – 26384Doublecortin 2
Compositional bias287 – 34256Pro/Ser-rich

Amino acid modifications

Modified residue281Phosphoserine; by CDK5 Ref.3
Modified residue471Phosphoserine; by MARK1 and PKA By similarity
Modified residue701Phosphotyrosine; by ABL Potential
Modified residue741Phosphoserine; by PKC Potential
Modified residue901Phosphoserine; by CK2 Potential
Modified residue1101Phosphoserine; by PKC Potential
Modified residue1151Phosphoserine; by CK2, MARK1 and PKA By similarity
Modified residue2651Phosphoserine; by CK2 Ref.6
Modified residue2871Phosphoserine; by CDK5 Ref.3
Modified residue2891Phosphothreonine; by CDK5 Ref.3
Modified residue2941Phosphoserine; by PKC Potential
Modified residue2971Phosphoserine; alternate Ref.3 Ref.6
Modified residue2971Phosphoserine; by CDK5; alternate Ref.3 Ref.6
Modified residue3061Phosphoserine; alternate Ref.5
Modified residue3061Phosphoserine; by CK2; alternate Potential
Modified residue3061Phosphoserine; by DYRK2; alternate By similarity
Modified residue3261Phosphothreonine; by CDK5; alternate Ref.3
Modified residue3261Phosphothreonine; by PKC and MAPK; alternate Potential
Modified residue3321Phosphoserine; by CDK5; alternate Ref.3
Modified residue3321Phosphoserine; by MAPK; alternate Potential
Modified residue3361Phosphothreonine; by MAPK Potential
Modified residue3391Phosphoserine; by CDK5; alternate Ref.3
Modified residue3391Phosphoserine; by MAPK; alternate Potential
Modified residue3421Phosphoserine; by PKC Potential
Modified residue3551Phosphoserine; by CK2 Potential
Modified residue3611Phosphoserine; by CK2 Potential

Experimental info

Mutagenesis281S → A: Reduces overall phosphorylation by 25%. Ref.3
Mutagenesis2871S → A: No effect on overall phosphorylation. Ref.3
Mutagenesis2891T → A: No effect on overall phosphorylation. Ref.3
Mutagenesis2971S → A: No effect on overall phosphorylation. Ref.3
Mutagenesis3261T → A: No effect on overall phosphorylation. Ref.3
Mutagenesis3321S → A: No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-339. Ref.3
Mutagenesis3361T → A: No effect on overall phosphorylation. Ref.3
Mutagenesis3391S → A: No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-332. Ref.3
Sequence conflict3451K → R in AAC31799. Ref.2
Sequence conflict3481Missing in AAC31799. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O88809 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 323D0774C222F295

FASTA36640,613
        10         20         30         40         50         60 
MELDFGHFDE RDKASRNMRG SRMNGLPSPT HSAHCSFYRT RTLQALSNEK KAKKVRFYRN 

        70         80         90        100        110        120 
GDRYFKGIVY AVSSDRFRSF DALLADLTRS LSDNINLPQG VRYIYTIDGS RKIGSMDELE 

       130        140        150        160        170        180 
EGESYVCSSD NFFKKVEYTK NVNPNWSVNV KTSANMKAPQ SLASSNSAQA RENKDFVRPK 

       190        200        210        220        230        240 
LVTIIRSGVK PRKAVRVLLN KKTAHSFEQV LTDITEAIKL ETGVVKKLYT LDGKQVTCLH 

       250        260        270        280        290        300 
DFFGDDDVFI ACGPEKFRYA QDDFSLDENE CRVMKGNPSA AAGPKASPTP QKTSAKSPGP 

       310        320        330        340        350        360 
MRRSKSPADS GNDQDANGTS SSQLSTPKSK QSPISTPTSP GSLRKHKVDL YLPLSLDDSD 


SLGDSM 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and developmental expression of the murine homolog of doublecortin."
Matsuo N., Kawamoto S., Matsubara K., Okubo K.
Biochem. Biophys. Res. Commun. 252:571-576(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Hippocampus.
[2]"Human doublecortin (DCX) and the homologous gene in mouse encode a putative Ca2+-dependent signaling protein which is mutated in human X-linked neuronal migration defects."
Sossey-Alaoui K., Hartung A.J., Guerrini R., Manchester D.K., Posar A., Puche-Mira A., Andermann E., Dobyns W.B., Srivastava A.K.
Hum. Mol. Genet. 7:1327-1332(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[3]"Multisite phosphorylation of doublecortin by cyclin-dependent kinase 5."
Graham M.E., Ruma-Haynes P., Capes-Davis A.G., Dunn J.M., Tan T.C., Valova V.A., Robinson P.J., Jeffrey P.L.
Biochem. J. 381:471-481(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-28; SER-287; THR-289; SER-297; THR-326; SER-332 AND SER-339, MUTAGENESIS OF SER-28; SER-287; THR-289; SER-297; THR-326; SER-332; THR-336 AND SER-339.
Strain: Swiss Webster.
Tissue: Embryo.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Comparative phosphoproteomic analysis of neonatal and adult murine brain."
Goswami T., Li X., Smith A.M., Luderowski E.M., Vincent J.J., Rush J., Ballif B.A.
Proteomics 12:2185-2189(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-265 AND SER-297.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011678 mRNA. Translation: BAA33387.1.
AF045547 mRNA. Translation: AAC31799.1.
AY560329 mRNA. Translation: AAT58219.1.
BX530055 Genomic DNA. Translation: CAM25089.1.
CCDSCCDS53209.1.
PIRJE0368.
RefSeqNP_001103692.1. NM_001110222.1.
NP_001103693.1. NM_001110223.1.
NP_001103694.1. NM_001110224.1.
NP_034155.2. NM_010025.2.
UniGeneMm.12871.

3D structure databases

ProteinModelPortalO88809.
SMRO88809. Positions 51-156, 167-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199073. 5 interactions.
MINTMINT-216915.

PTM databases

PhosphoSiteO88809.

Proteomic databases

MaxQBO88809.
PaxDbO88809.
PRIDEO88809.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033642; ENSMUSP00000033642; ENSMUSG00000031285.
ENSMUST00000087313; ENSMUSP00000084570; ENSMUSG00000031285.
GeneID13193.
KEGGmmu:13193.
UCSCuc009umn.1. mouse.

Organism-specific databases

CTD1641.
MGIMGI:1277171. Dcx.

Phylogenomic databases

eggNOGNOG238212.
GeneTreeENSGT00680000099865.
HOVERGENHBG003790.
InParanoidO88809.
KOK16579.
OMACRVMKGS.
OrthoDBEOG7M98FQ.
PhylomeDBO88809.
TreeFamTF318770.

Gene expression databases

ArrayExpressO88809.
BgeeO88809.
CleanExMM_DCN.
MM_DCX.
GenevestigatorO88809.

Family and domain databases

Gene3D3.10.20.230. 2 hits.
InterProIPR017302. Doublecortin_chordata.
IPR003533. Doublecortin_dom.
[Graphical view]
PfamPF03607. DCX. 2 hits.
[Graphical view]
PIRSFPIRSF037870. Doublin. 1 hit.
SMARTSM00537. DCX. 2 hits.
[Graphical view]
PROSITEPS50309. DC. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDCX. mouse.
NextBio283316.
PROO88809.
SOURCESearch...

Entry information

Entry nameDCX_MOUSE
AccessionPrimary (citable) accession number: O88809
Secondary accession number(s): Q6E5A4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot