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O88809

- DCX_MOUSE

UniProt

O88809 - DCX_MOUSE

Protein

Neuronal migration protein doublecortin

Gene

Dcx

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration By similarity.By similarity

    GO - Molecular functioni

    1. microtubule binding Source: UniProtKB
    2. protein binding Source: MGI

    GO - Biological processi

    1. axon extension Source: MGI
    2. brain development Source: MGI
    3. central nervous system projection neuron axonogenesis Source: MGI
    4. dendrite morphogenesis Source: MGI
    5. intracellular signal transduction Source: InterPro
    6. neuron migration Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal migration protein doublecortin
    Alternative name(s):
    Doublin
    Lissencephalin-X
    Short name:
    Lis-X
    Gene namesi
    Name:Dcx
    Synonyms:Dcn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1277171. Dcx.

    Subcellular locationi

    Cytoplasm Curated. Cell projection By similarity
    Note: Localizes at neurite tips.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. intracellular Source: MGI
    3. microtubule Source: UniProtKB-KW
    4. neuron projection Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Microtubule

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi28 – 281S → A: Reduces overall phosphorylation by 25%. 1 Publication
    Mutagenesisi287 – 2871S → A: No effect on overall phosphorylation. 1 Publication
    Mutagenesisi289 – 2891T → A: No effect on overall phosphorylation. 1 Publication
    Mutagenesisi297 – 2971S → A: No effect on overall phosphorylation. 1 Publication
    Mutagenesisi326 – 3261T → A: No effect on overall phosphorylation. 1 Publication
    Mutagenesisi332 – 3321S → A: No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-339. 1 Publication
    Mutagenesisi336 – 3361T → A: No effect on overall phosphorylation. 1 Publication
    Mutagenesisi339 – 3391S → A: No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-332. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Neuronal migration protein doublecortinPRO_0000079834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Phosphoserine; by CDK51 Publication
    Modified residuei47 – 471Phosphoserine; by MARK1 and PKABy similarity
    Modified residuei70 – 701Phosphotyrosine; by ABLSequence Analysis
    Modified residuei74 – 741Phosphoserine; by PKCSequence Analysis
    Modified residuei90 – 901Phosphoserine; by CK2Sequence Analysis
    Modified residuei110 – 1101Phosphoserine; by PKCSequence Analysis
    Modified residuei115 – 1151Phosphoserine; by CK2, MARK1 and PKABy similarity
    Modified residuei265 – 2651Phosphoserine; by CK21 Publication
    Modified residuei287 – 2871Phosphoserine; by CDK51 Publication
    Modified residuei289 – 2891Phosphothreonine; by CDK51 Publication
    Modified residuei294 – 2941Phosphoserine; by PKCSequence Analysis
    Modified residuei297 – 2971Phosphoserine; alternate2 Publications
    Modified residuei297 – 2971Phosphoserine; by CDK5; alternate2 Publications
    Modified residuei306 – 3061Phosphoserine; alternate1 Publication
    Modified residuei306 – 3061Phosphoserine; by CK2; alternateSequence Analysis
    Modified residuei306 – 3061Phosphoserine; by DYRK2; alternateBy similarity
    Modified residuei326 – 3261Phosphothreonine; by CDK5; alternate1 Publication
    Modified residuei326 – 3261Phosphothreonine; by PKC and MAPK; alternateSequence Analysis
    Modified residuei332 – 3321Phosphoserine; by CDK5; alternate1 Publication
    Modified residuei332 – 3321Phosphoserine; by MAPK; alternateSequence Analysis
    Modified residuei336 – 3361Phosphothreonine; by MAPKSequence Analysis
    Modified residuei339 – 3391Phosphoserine; by CDK5; alternate1 Publication
    Modified residuei339 – 3391Phosphoserine; by MAPK; alternateSequence Analysis
    Modified residuei342 – 3421Phosphoserine; by PKCSequence Analysis
    Modified residuei355 – 3551Phosphoserine; by CK2Sequence Analysis
    Modified residuei361 – 3611Phosphoserine; by CK2Sequence Analysis

    Post-translational modificationi

    Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules.By similarity
    Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO88809.
    PaxDbiO88809.
    PRIDEiO88809.

    PTM databases

    PhosphoSiteiO88809.

    Expressioni

    Tissue specificityi

    In neonatal tissues, highly expressed in brain, but not expressed in heart, liver, kidney and spleen. In adult tissues, faintly expressed in brain but not expressed in muscle, heart, lung, liver, spleen, intestine, kidney, testis and placenta.

    Developmental stagei

    Already expressed by embryonic day 11 (E11), maximally around birth, expression decreasing gradually during the second postnatal week, with no expression in adult stages.

    Gene expression databases

    ArrayExpressiO88809.
    BgeeiO88809.
    CleanExiMM_DCN.
    MM_DCX.
    GenevestigatoriO88809.

    Interactioni

    Subunit structurei

    Interacts with tubulin. Interacts with USP9X.By similarity

    Protein-protein interaction databases

    BioGridi199073. 5 interactions.
    MINTiMINT-216915.

    Structurei

    3D structure databases

    ProteinModelPortaliO88809.
    SMRiO88809. Positions 51-156, 167-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 13987Doublecortin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini180 – 26384Doublecortin 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi287 – 34256Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 doublecortin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG238212.
    GeneTreeiENSGT00680000099865.
    HOVERGENiHBG003790.
    InParanoidiO88809.
    KOiK16579.
    OMAiCRVMKGS.
    OrthoDBiEOG7M98FQ.
    PhylomeDBiO88809.
    TreeFamiTF318770.

    Family and domain databases

    Gene3Di3.10.20.230. 2 hits.
    InterProiIPR017302. Doublecortin_chordata.
    IPR003533. Doublecortin_dom.
    [Graphical view]
    PfamiPF03607. DCX. 2 hits.
    [Graphical view]
    PIRSFiPIRSF037870. Doublin. 1 hit.
    SMARTiSM00537. DCX. 2 hits.
    [Graphical view]
    PROSITEiPS50309. DC. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88809-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELDFGHFDE RDKASRNMRG SRMNGLPSPT HSAHCSFYRT RTLQALSNEK    50
    KAKKVRFYRN GDRYFKGIVY AVSSDRFRSF DALLADLTRS LSDNINLPQG 100
    VRYIYTIDGS RKIGSMDELE EGESYVCSSD NFFKKVEYTK NVNPNWSVNV 150
    KTSANMKAPQ SLASSNSAQA RENKDFVRPK LVTIIRSGVK PRKAVRVLLN 200
    KKTAHSFEQV LTDITEAIKL ETGVVKKLYT LDGKQVTCLH DFFGDDDVFI 250
    ACGPEKFRYA QDDFSLDENE CRVMKGNPSA AAGPKASPTP QKTSAKSPGP 300
    MRRSKSPADS GNDQDANGTS SSQLSTPKSK QSPISTPTSP GSLRKHKVDL 350
    YLPLSLDDSD SLGDSM 366
    Length:366
    Mass (Da):40,613
    Last modified:November 1, 1998 - v1
    Checksum:i323D0774C222F295
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti345 – 3451K → R in AAC31799. (PubMed:9668176)Curated
    Sequence conflicti348 – 3481Missing in AAC31799. (PubMed:9668176)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011678 mRNA. Translation: BAA33387.1.
    AF045547 mRNA. Translation: AAC31799.1.
    AY560329 mRNA. Translation: AAT58219.1.
    BX530055 Genomic DNA. Translation: CAM25089.1.
    CCDSiCCDS53209.1.
    PIRiJE0368.
    RefSeqiNP_001103692.1. NM_001110222.1.
    NP_001103693.1. NM_001110223.1.
    NP_001103694.1. NM_001110224.1.
    NP_034155.2. NM_010025.2.
    UniGeneiMm.12871.

    Genome annotation databases

    EnsembliENSMUST00000033642; ENSMUSP00000033642; ENSMUSG00000031285.
    ENSMUST00000087313; ENSMUSP00000084570; ENSMUSG00000031285.
    GeneIDi13193.
    KEGGimmu:13193.
    UCSCiuc009umn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011678 mRNA. Translation: BAA33387.1 .
    AF045547 mRNA. Translation: AAC31799.1 .
    AY560329 mRNA. Translation: AAT58219.1 .
    BX530055 Genomic DNA. Translation: CAM25089.1 .
    CCDSi CCDS53209.1.
    PIRi JE0368.
    RefSeqi NP_001103692.1. NM_001110222.1.
    NP_001103693.1. NM_001110223.1.
    NP_001103694.1. NM_001110224.1.
    NP_034155.2. NM_010025.2.
    UniGenei Mm.12871.

    3D structure databases

    ProteinModelPortali O88809.
    SMRi O88809. Positions 51-156, 167-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199073. 5 interactions.
    MINTi MINT-216915.

    PTM databases

    PhosphoSitei O88809.

    Proteomic databases

    MaxQBi O88809.
    PaxDbi O88809.
    PRIDEi O88809.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033642 ; ENSMUSP00000033642 ; ENSMUSG00000031285 .
    ENSMUST00000087313 ; ENSMUSP00000084570 ; ENSMUSG00000031285 .
    GeneIDi 13193.
    KEGGi mmu:13193.
    UCSCi uc009umn.1. mouse.

    Organism-specific databases

    CTDi 1641.
    MGIi MGI:1277171. Dcx.

    Phylogenomic databases

    eggNOGi NOG238212.
    GeneTreei ENSGT00680000099865.
    HOVERGENi HBG003790.
    InParanoidi O88809.
    KOi K16579.
    OMAi CRVMKGS.
    OrthoDBi EOG7M98FQ.
    PhylomeDBi O88809.
    TreeFami TF318770.

    Miscellaneous databases

    ChiTaRSi DCX. mouse.
    NextBioi 283316.
    PROi O88809.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88809.
    Bgeei O88809.
    CleanExi MM_DCN.
    MM_DCX.
    Genevestigatori O88809.

    Family and domain databases

    Gene3Di 3.10.20.230. 2 hits.
    InterProi IPR017302. Doublecortin_chordata.
    IPR003533. Doublecortin_dom.
    [Graphical view ]
    Pfami PF03607. DCX. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF037870. Doublin. 1 hit.
    SMARTi SM00537. DCX. 2 hits.
    [Graphical view ]
    PROSITEi PS50309. DC. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and developmental expression of the murine homolog of doublecortin."
      Matsuo N., Kawamoto S., Matsubara K., Okubo K.
      Biochem. Biophys. Res. Commun. 252:571-576(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Hippocampus.
    2. "Human doublecortin (DCX) and the homologous gene in mouse encode a putative Ca2+-dependent signaling protein which is mutated in human X-linked neuronal migration defects."
      Sossey-Alaoui K., Hartung A.J., Guerrini R., Manchester D.K., Posar A., Puche-Mira A., Andermann E., Dobyns W.B., Srivastava A.K.
      Hum. Mol. Genet. 7:1327-1332(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-28; SER-287; THR-289; SER-297; THR-326; SER-332 AND SER-339, MUTAGENESIS OF SER-28; SER-287; THR-289; SER-297; THR-326; SER-332; THR-336 AND SER-339.
      Strain: Swiss Webster.
      Tissue: Embryo.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    6. "Comparative phosphoproteomic analysis of neonatal and adult murine brain."
      Goswami T., Li X., Smith A.M., Luderowski E.M., Vincent J.J., Rush J., Ballif B.A.
      Proteomics 12:2185-2189(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-265 AND SER-297.

    Entry informationi

    Entry nameiDCX_MOUSE
    AccessioniPrimary (citable) accession number: O88809
    Secondary accession number(s): Q6E5A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3