O88801 (HOME2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homer protein homolog 2
Short name=Homer-2

Alternative name(s):
Cupidin
VASP/Ena-related gene up-regulated during seizure and LTP 2
Short name=Vesl-2

Gene names

Name:	Homer2
Synonyms:	Vesl2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Subunit structure	Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Subcellular location	Cytoplasm. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface.
Tissue specificity	Constitutively expressed in the adult hippocampus.
Developmental stage	In the developing hippocampus, expression is high at P8, then decreased along with hippocampal development.
Domain	The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell neuronal cell body Inferred from direct assay. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled glutamate receptor binding Inferred from physical interaction. Source: UniProtKB protein domain specific binding Inferred from mutant phenotype. Source: RGD protein heterodimerization activity Inferred from physical interaction Ref.1. Source: RGD protein homodimerization activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O88801-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O88801-2)

Also known as: Vesl-2(delta 11);

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Homer protein homolog 2		PRO_0000191010
Regions
	Domain	1 – 110	110	WH1
	Coiled coil	92 – 120	29	Potential
	Coiled coil	160 – 329	170	Potential
Natural variations
	Alternative sequence	130 – 140	11	Missing in isoform 2.		VSP_009072

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 205FA6358F9D198E	FASTA	354	40,563
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPST KKNWVPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVREAA RLARDKSQEK 130 140 150 160 170 180 IETSSNHSQE SGCETPSSTQ ASSVNGTDDE KASHASPADT HLKSENDKLK IALTQSAANV 190 200 210 220 230 240 KKWEIELQTL RESNARLTTA LQESAASVEQ WKRQFSICRD ENDRLRSKIE ELEEQCGEIN 250 260 270 280 290 300 REKEKNTQLK RRIEELESEV REKEMELKDL RKQSEIIPQL MSECEYVSEK LEAAERDNQN 310 320 330 340 350 LEDKVRSLKT DIEESKYRQR HLKGELKSFL EVLDGKIDDL HDFRRGLSKL GTDN
	Isoform 2 (Vesl-2(delta 11)) [UniParc]. Checksum: 4A96526D4BECBFCB	FASTA	343	39,456
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPST KKNWVPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVREAA RLARDKSQEK 130 140 150 160 170 180 IETSSNHSQA SSVNGTDDEK ASHASPADTH LKSENDKLKI ALTQSAANVK KWEIELQTLR 190 200 210 220 230 240 ESNARLTTAL QESAASVEQW KRQFSICRDE NDRLRSKIEE LEEQCGEINR EKEKNTQLKR 250 260 270 280 290 300 RIEELESEVR EKEMELKDLR KQSEIIPQLM SECEYVSEKL EAAERDNQNL EDKVRSLKTD 310 320 330 340 IEESKYRQRH LKGELKSFLE VLDGKIDDLH DFRRGLSKLG TDN

References

[1]	"Novel members of the Vesl/Homer family of PDZ-proteins that bind metabotropic glutamate receptors." Kato A., Ozawa F., Saitoh Y., Fukazawa Y., Sugiyama H., Inokuchi K. J. Biol. Chem. 273:23969-23975(1998) [PubMed: 9727012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: Sprague-Dawley. Tissue: Hippocampus.
[2]	"PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis." Rong R., Ahn J.-Y., Huang H., Nagata E., Kalman D., Kapp J.A., Tu J., Worley P.F., Snyder S.H., Ye K. Nat. Neurosci. 6:1153-1161(2003) [PubMed: 14528310] [Abstract] Cited for: INTERACTION WITH AGAP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007689 mRNA. Translation: BAA32478.1. AB007690 mRNA. Translation: BAA32479.1.
IPI	IPI00213180. IPI00395271.
RefSeq	NP_445761.1. NM_053309.1.
UniGene	Rn.30014.
3D structure databases
ProteinModelPortal	O88801.
SMR	O88801. Positions 1-131.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88801. 2 interactions.
STRING	O88801.
Proteomic databases
PRIDE	O88801.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026158; ENSRNOP00000026158; ENSRNOG00000019297.
GeneID	29547.
KEGG	rno:29547.
UCSC	AB007690. rat. NM_053309. rat.
Organism-specific databases
CTD	9455.
RGD	620705. Homer2.
Phylogenomic databases
eggNOG	roNOG12514.
GeneTree	ENSGT00390000017850.
HOVERGEN	HBG051918.
OrthoDB	EOG4DFPNT.
PhylomeDB	O88801.
Gene expression databases
ArrayExpress	O88801.
Genevestigator	O88801.
GermOnline	ENSRNOG00000019297. Rattus norvegicus.
Family and domain databases
InterPro	IPR000697. EVH1. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K15010.
Pfam	PF00568. WH1. 1 hit. [Graphical view]
SMART	SM00461. WH1. 1 hit. [Graphical view]
PROSITE	PS50229. WH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609567.

Entry information

Entry name

HOME2_RAT

Accession

Primary (citable) accession number: O88801
Secondary accession number(s): O88802

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program