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Protein

Pyridoxine-5'-phosphate oxidase

Gene

Pnpo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).1 Publication

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.

Cofactori

FMNNote: Binds 1 FMN per subunit.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine-5'-phosphate oxidase (Pnpo)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine-5'-phosphate oxidase (Pnpo)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100Pyridoxal 5'-phosphateBy similarity1
Binding sitei139FMNBy similarity1
Binding sitei157Pyridoxal 5'-phosphateBy similarity1
Binding sitei161Pyridoxal 5'-phosphateBy similarity1
Binding sitei165Pyridoxal 5'-phosphateBy similarity1
Binding sitei219FMNBy similarity1
Binding sitei229FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 98FMNBy similarity4
Nucleotide bindingi110 – 111FMNBy similarity2
Nucleotide bindingi116 – 117FMNBy similarity2
Nucleotide bindingi174 – 175FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandFlavoprotein, FMN, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-RNO-964975 Vitamins B6 activation to pyridoxal phosphate
UniPathwayiUPA01068; UER00304
UPA01068; UER00305

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
Alternative name(s):
Pyridoxamine-phosphate oxidase
Gene namesi
Name:Pnpo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621456 Pnpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001677851 – 261Pyridoxine-5'-phosphate oxidaseAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei238PhosphothreonineBy similarity1
Modified residuei241PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88794
PRIDEiO88794

PTM databases

iPTMnetiO88794
PhosphoSitePlusiO88794
SwissPalmiO88794

Expressioni

Tissue specificityi

Detected in adult liver.1 Publication

Developmental stagei

Detected at low levels in fetal brain, and at high levels in adult brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000046493
GenevisibleiO88794 RN

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067069

Structurei

3D structure databases

ProteinModelPortaliO88794
SMRiO88794
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 45Pyridoxal 5'-phosphate bindingBy similarity4
Regioni225 – 227Pyridoxal 5'-phosphate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2586 Eukaryota
COG0259 LUCA
GeneTreeiENSGT00390000011219
HOVERGENiHBG045634
InParanoidiO88794
KOiK00275
OMAiPEPNAMV
OrthoDBiEOG091G0J0R
PhylomeDBiO88794

Family and domain databases

Gene3Di2.30.110.10, 1 hit
HAMAPiMF_01629 PdxH, 1 hit
InterProiView protein in InterPro
IPR000659 Pyridox_Oxase
IPR019740 Pyridox_Oxase_CS
IPR011576 Pyridox_Oxase_put
IPR019576 Pyridoxamine_oxidase_dimer_C
IPR012349 Split_barrel_FMN-bd
PANTHERiPTHR10851:SF0 PTHR10851:SF0, 1 hit
PfamiView protein in Pfam
PF10590 PNP_phzG_C, 1 hit
PF01243 Putative_PNPOx, 1 hit
PIRSFiPIRSF000190 Pyd_amn-ph_oxd, 1 hit
TIGRFAMsiTIGR00558 pdxH, 1 hit
PROSITEiView protein in PROSITE
PS01064 PYRIDOX_OXIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

O88794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTCGLLSVTV TFRRPAKWPG YFRHLCCRGA VMDLGPMRKS YRGDREAFEE
60 70 80 90 100
AHLTSLDPMK QFASWFEEAV QCPDIGEANA MCLATCTRDG KPSARMLLLK
110 120 130 140 150
GFGKDGFRFF TNYESRKGKE LDSNPFASLV FYWEPLNRQV RVEGPVKKLP
160 170 180 190 200
EKEAENYFHS RPKSSQIGAV VSRQSSVIPD REYLRKKNEE LGQLYREQEV
210 220 230 240 250
PKPEYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLATGD SPLGPMTHHG
260
EEDWVYERLA P
Length:261
Mass (Da):30,184
Last modified:November 1, 1998 - v1
Checksum:iC5684A180FE89B68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91561 mRNA Translation: AAC23707.1
BC087016 mRNA Translation: AAH87016.1
RefSeqiNP_072123.1, NM_022601.2
UniGeneiRn.158883

Genome annotation databases

EnsembliENSRNOT00000073746; ENSRNOP00000067069; ENSRNOG00000046493
GeneIDi64533
KEGGirno:64533

Similar proteinsi

Entry informationi

Entry nameiPNPO_RAT
AccessioniPrimary (citable) accession number: O88794
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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