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O88794

- PNPO_RAT

UniProt

O88794 - PNPO_RAT

Protein

Pyridoxine-5'-phosphate oxidase

Gene

Pnpo

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).1 Publication

    Catalytic activityi

    Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.
    Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.

    Cofactori

    Binds 1 FMN per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951FMNBy similarity
    Binding sitei98 – 981FMN; via amide nitrogenBy similarity
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei117 – 1171FMNBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei161 – 1611SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1112FMNBy similarity
    Nucleotide bindingi174 – 1752FMNBy similarity

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. pyridoxamine-phosphate oxidase activity Source: RGD

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: GOC
    2. pyridoxine biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN, Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_199033. Vitamins B6 activation to pyridoxal phosphate.
    UniPathwayiUPA00190; UER00304.
    UPA00190; UER00305.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
    Alternative name(s):
    Pyridoxamine-phosphate oxidase
    Gene namesi
    Name:Pnpo
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi621456. Pnpo.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Pyridoxine-5'-phosphate oxidasePRO_0000167785Add
    BLAST

    Proteomic databases

    PRIDEiO88794.

    Expressioni

    Tissue specificityi

    Detected in adult liver.1 Publication

    Developmental stagei

    Detected at low levels in fetal brain, and at high levels in adult brain.1 Publication

    Gene expression databases

    GenevestigatoriO88794.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO88794.
    SMRiO88794. Positions 49-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni225 – 2273Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000011219.
    HOVERGENiHBG045634.
    KOiK00275.
    OMAiELDHRGF.
    OrthoDBiEOG7B8S4P.
    PhylomeDBiO88794.

    Family and domain databases

    Gene3Di2.30.110.10. 1 hit.
    HAMAPiMF_01629. PdxH.
    InterProiIPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view]
    PANTHERiPTHR10851. PTHR10851. 1 hit.
    PfamiPF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMiSSF50475. SSF50475. 1 hit.
    TIGRFAMsiTIGR00558. pdxH. 1 hit.
    PROSITEiPS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88794-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTCGLLSVTV TFRRPAKWPG YFRHLCCRGA VMDLGPMRKS YRGDREAFEE    50
    AHLTSLDPMK QFASWFEEAV QCPDIGEANA MCLATCTRDG KPSARMLLLK 100
    GFGKDGFRFF TNYESRKGKE LDSNPFASLV FYWEPLNRQV RVEGPVKKLP 150
    EKEAENYFHS RPKSSQIGAV VSRQSSVIPD REYLRKKNEE LGQLYREQEV 200
    PKPEYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLATGD SPLGPMTHHG 250
    EEDWVYERLA P 261
    Length:261
    Mass (Da):30,184
    Last modified:November 1, 1998 - v1
    Checksum:iC5684A180FE89B68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U91561 mRNA. Translation: AAC23707.1.
    BC087016 mRNA. Translation: AAH87016.1.
    RefSeqiNP_072123.1. NM_022601.2.
    UniGeneiRn.158883.

    Genome annotation databases

    EnsembliENSRNOT00000073746; ENSRNOP00000067069; ENSRNOG00000046493.
    GeneIDi64533.
    KEGGirno:64533.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U91561 mRNA. Translation: AAC23707.1 .
    BC087016 mRNA. Translation: AAH87016.1 .
    RefSeqi NP_072123.1. NM_022601.2.
    UniGenei Rn.158883.

    3D structure databases

    ProteinModelPortali O88794.
    SMRi O88794. Positions 49-261.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O88794.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000073746 ; ENSRNOP00000067069 ; ENSRNOG00000046493 .
    GeneIDi 64533.
    KEGGi rno:64533.

    Organism-specific databases

    CTDi 55163.
    RGDi 621456. Pnpo.

    Phylogenomic databases

    GeneTreei ENSGT00390000011219.
    HOVERGENi HBG045634.
    KOi K00275.
    OMAi ELDHRGF.
    OrthoDBi EOG7B8S4P.
    PhylomeDBi O88794.

    Enzyme and pathway databases

    UniPathwayi UPA00190 ; UER00304 .
    UPA00190 ; UER00305 .
    Reactomei REACT_199033. Vitamins B6 activation to pyridoxal phosphate.

    Miscellaneous databases

    NextBioi 613378.
    PROi O88794.

    Gene expression databases

    Genevestigatori O88794.

    Family and domain databases

    Gene3Di 2.30.110.10. 1 hit.
    HAMAPi MF_01629. PdxH.
    InterProi IPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view ]
    PANTHERi PTHR10851. PTHR10851. 1 hit.
    Pfami PF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMi SSF50475. SSF50475. 1 hit.
    TIGRFAMsi TIGR00558. pdxH. 1 hit.
    PROSITEi PS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Absence of pyridoxine-5'-phosphate oxidase (PNPO) activity in neoplastic cells: isolation, characterization, and expression of PNPO cDNA."
      Ngo E.O., LePage G.R., Thanassi J.W., Meisler N., Nutter L.M.
      Biochemistry 37:7741-7748(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 109-116; 164-181 AND 188-196, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiPNPO_RAT
    AccessioniPrimary (citable) accession number: O88794
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3