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O88794

- PNPO_RAT

UniProt

O88794 - PNPO_RAT

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Protein
Pyridoxine-5'-phosphate oxidase
Gene
Pnpo
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).1 Publication

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.UniRule annotation
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.UniRule annotation

Cofactori

Binds 1 FMN per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951FMN By similarity
Binding sitei98 – 981FMN; via amide nitrogen By similarity
Binding sitei100 – 1001Substrate By similarity
Binding sitei117 – 1171FMN By similarity
Binding sitei157 – 1571Substrate By similarity
Binding sitei161 – 1611Substrate By similarity
Binding sitei165 – 1651Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1112FMN By similarity
Nucleotide bindingi174 – 1752FMN By similarity

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. pyridoxamine-phosphate oxidase activity Source: RGD

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: GOC
  2. pyridoxine biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_199033. Vitamins B6 activation to pyridoxal phosphate.
UniPathwayiUPA00190; UER00304.
UPA00190; UER00305.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
Alternative name(s):
Pyridoxamine-phosphate oxidase
Gene namesi
Name:Pnpo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi621456. Pnpo.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Pyridoxine-5'-phosphate oxidaseUniRule annotation
PRO_0000167785Add
BLAST

Proteomic databases

PRIDEiO88794.

Expressioni

Tissue specificityi

Detected in adult liver.1 Publication

Developmental stagei

Detected at low levels in fetal brain, and at high levels in adult brain.1 Publication

Gene expression databases

GenevestigatoriO88794.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO88794.
SMRiO88794. Positions 49-261.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2273Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011219.
HOVERGENiHBG045634.
KOiK00275.
OMAiELDHRGF.
OrthoDBiEOG7B8S4P.
PhylomeDBiO88794.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_01629. PdxH.
InterProiIPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_FMN-bd.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PANTHERiPTHR10851. PTHR10851. 1 hit.
PfamiPF10590. PNPOx_C. 1 hit.
PF01243. Pyridox_oxidase. 1 hit.
[Graphical view]
PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR00558. pdxH. 1 hit.
PROSITEiPS01064. PYRIDOX_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88794-1 [UniParc]FASTAAdd to Basket

« Hide

MTCGLLSVTV TFRRPAKWPG YFRHLCCRGA VMDLGPMRKS YRGDREAFEE    50
AHLTSLDPMK QFASWFEEAV QCPDIGEANA MCLATCTRDG KPSARMLLLK 100
GFGKDGFRFF TNYESRKGKE LDSNPFASLV FYWEPLNRQV RVEGPVKKLP 150
EKEAENYFHS RPKSSQIGAV VSRQSSVIPD REYLRKKNEE LGQLYREQEV 200
PKPEYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLATGD SPLGPMTHHG 250
EEDWVYERLA P 261
Length:261
Mass (Da):30,184
Last modified:November 1, 1998 - v1
Checksum:iC5684A180FE89B68
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U91561 mRNA. Translation: AAC23707.1.
BC087016 mRNA. Translation: AAH87016.1.
RefSeqiNP_072123.1. NM_022601.2.
UniGeneiRn.158883.

Genome annotation databases

EnsembliENSRNOT00000073746; ENSRNOP00000067069; ENSRNOG00000046493.
GeneIDi64533.
KEGGirno:64533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U91561 mRNA. Translation: AAC23707.1 .
BC087016 mRNA. Translation: AAH87016.1 .
RefSeqi NP_072123.1. NM_022601.2.
UniGenei Rn.158883.

3D structure databases

ProteinModelPortali O88794.
SMRi O88794. Positions 49-261.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O88794.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000073746 ; ENSRNOP00000067069 ; ENSRNOG00000046493 .
GeneIDi 64533.
KEGGi rno:64533.

Organism-specific databases

CTDi 55163.
RGDi 621456. Pnpo.

Phylogenomic databases

GeneTreei ENSGT00390000011219.
HOVERGENi HBG045634.
KOi K00275.
OMAi ELDHRGF.
OrthoDBi EOG7B8S4P.
PhylomeDBi O88794.

Enzyme and pathway databases

UniPathwayi UPA00190 ; UER00304 .
UPA00190 ; UER00305 .
Reactomei REACT_199033. Vitamins B6 activation to pyridoxal phosphate.

Miscellaneous databases

NextBioi 613378.
PROi O88794.

Gene expression databases

Genevestigatori O88794.

Family and domain databases

Gene3Di 2.30.110.10. 1 hit.
HAMAPi MF_01629. PdxH.
InterProi IPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_FMN-bd.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
[Graphical view ]
PANTHERi PTHR10851. PTHR10851. 1 hit.
Pfami PF10590. PNPOx_C. 1 hit.
PF01243. Pyridox_oxidase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMi SSF50475. SSF50475. 1 hit.
TIGRFAMsi TIGR00558. pdxH. 1 hit.
PROSITEi PS01064. PYRIDOX_OXIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Absence of pyridoxine-5'-phosphate oxidase (PNPO) activity in neoplastic cells: isolation, characterization, and expression of PNPO cDNA."
    Ngo E.O., LePage G.R., Thanassi J.W., Meisler N., Nutter L.M.
    Biochemistry 37:7741-7748(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 109-116; 164-181 AND 188-196, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiPNPO_RAT
AccessioniPrimary (citable) accession number: O88794
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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