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Protein

Junctional adhesion molecule A

Gene

F11r

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11447115). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed:11447115). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (PubMed:9660867). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (By similarity). Involved in platelet activation (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • actomyosin structure organization Source: MGI
  • cell adhesion Source: MGI
  • epithelial cell differentiation Source: MGI
  • establishment of endothelial intestinal barrier Source: MGI
  • establishment of protein localization to plasma membrane Source: MGI
  • intestinal absorption Source: UniProtKB
  • negative regulation of GTPase activity Source: MGI
  • positive regulation of blood pressure Source: Ensembl
  • positive regulation of GTPase activity Source: MGI
  • regulation of actin cytoskeleton reorganization Source: MGI
  • regulation of cytokine production Source: CACAO
  • regulation of membrane permeability Source: UniProtKB
  • response to radiation Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-MMU-420029. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule A
Short name:
JAM-A
Alternative name(s):
Junctional adhesion molecule 1
Short name:
JAM-1
CD_antigen: CD321
Gene namesi
Name:F11r
Synonyms:Jam1, Jcam, Jcam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1321398. F11r.

Subcellular locationi

  • Cell junctiontight junction 1 Publication
  • Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

  • Note: Localized at tight junctions of both epithelial and endothelial cells.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 238ExtracellularSequence analysisAdd BLAST212
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 299CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001506727 – 300Junctional adhesion molecule AAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...)1 Publication1
Disulfide bondi49 ↔ 108Combined sources1 Publication
Disulfide bondi152 ↔ 212Combined sources1 Publication
Glycosylationi185N-linked (GlcNAc...)2 Publications1
Modified residuei282PhosphoserineBy similarity1
Modified residuei285PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88792.
PeptideAtlasiO88792.
PRIDEiO88792.

PTM databases

iPTMnetiO88792.
PhosphoSitePlusiO88792.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038235.
CleanExiMM_F11R.
GenevisibleiO88792. MM.

Interactioni

Subunit structurei

Interacts with the ninth PDZ domain of MPDZ (By similarity). Interacts with the first PDZ domain of PARD3 (PubMed:11447115). The association between PARD3 and PARD6B probably disrupts this interaction (PubMed:11447115). Interacts with ITGAL (via I-domain) (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200861. 2 interactors.
DIPiDIP-41166N.
IntActiO88792. 2 interactors.
MINTiMINT-247376.
STRINGi10090.ENSMUSP00000041907.

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Beta strandi35 – 40Combined sources6
Beta strandi45 – 48Combined sources4
Beta strandi50 – 53Combined sources4
Beta strandi55 – 65Combined sources11
Beta strandi68 – 74Combined sources7
Helixi80 – 82Combined sources3
Turni83 – 85Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi92 – 96Combined sources5
Helixi100 – 102Combined sources3
Beta strandi104 – 112Combined sources9
Beta strandi115 – 129Combined sources15
Beta strandi135 – 137Combined sources3
Beta strandi140 – 143Combined sources4
Beta strandi148 – 153Combined sources6
Beta strandi162 – 167Combined sources6
Beta strandi170 – 173Combined sources4
Beta strandi184 – 186Combined sources3
Beta strandi188 – 190Combined sources3
Turni192 – 194Combined sources3
Beta strandi197 – 201Combined sources5
Helixi204 – 206Combined sources3
Beta strandi208 – 215Combined sources8
Beta strandi217 – 219Combined sources3
Beta strandi227 – 234Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F97X-ray2.50A27-238[»]
ProteinModelPortaliO88792.
SMRiO88792.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88792.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 122Ig-like V-type 1Add BLAST95
Domaini134 – 230Ig-like V-type 2Add BLAST97

Domaini

The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.By similarity

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWUE. Eukaryota.
ENOG410YHHV. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiO88792.
KOiK06089.
OMAiSCSYSGF.
OrthoDBiEOG091G05PI.
TreeFamiTF331459.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTEGKAGRK LLFLFTSMIL GSLVQGKGSV YTAQSDVQVP ENESIKLTCT
60 70 80 90 100
YSGFSSPRVE WKFVQGSTTA LVCYNSQITA PYADRVTFSS SGITFSSVTR
110 120 130 140 150
KDNGEYTCMV SEEGGQNYGE VSIHLTVLVP PSKPTISVPS SVTIGNRAVL
160 170 180 190 200
TCSEHDGSPP SEYSWFKDGI SMLTADAKKT RAFMNSSFTI DPKSGDLIFD
210 220 230 240 250
PVTAFDSGEY YCQAQNGYGT AMRSEAAHMD AVELNVGGIV AAVLVTLILL
260 270 280 290 300
GLLIFGVWFA YSRGYFERTK KGTAPGKKVI YSQPSTRSEG EFKQTSSFLV
Length:300
Mass (Da):32,424
Last modified:October 3, 2012 - v2
Checksum:i3CE561E8FF3B97EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268R → T in AAC32982 (PubMed:9660867).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89915 mRNA. Translation: AAC32982.1.
AK033574 mRNA. Translation: BAC28369.1.
CT010347 mRNA. Translation: CAJ18555.1.
AC087229 Genomic DNA. No translation available.
BC021876 mRNA. Translation: AAH21876.1.
CCDSiCCDS15496.1.
RefSeqiNP_766235.1. NM_172647.2.
UniGeneiMm.294882.

Genome annotation databases

EnsembliENSMUST00000043839; ENSMUSP00000041907; ENSMUSG00000038235.
GeneIDi16456.
KEGGimmu:16456.
UCSCiuc007doo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89915 mRNA. Translation: AAC32982.1.
AK033574 mRNA. Translation: BAC28369.1.
CT010347 mRNA. Translation: CAJ18555.1.
AC087229 Genomic DNA. No translation available.
BC021876 mRNA. Translation: AAH21876.1.
CCDSiCCDS15496.1.
RefSeqiNP_766235.1. NM_172647.2.
UniGeneiMm.294882.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F97X-ray2.50A27-238[»]
ProteinModelPortaliO88792.
SMRiO88792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200861. 2 interactors.
DIPiDIP-41166N.
IntActiO88792. 2 interactors.
MINTiMINT-247376.
STRINGi10090.ENSMUSP00000041907.

PTM databases

iPTMnetiO88792.
PhosphoSitePlusiO88792.

Proteomic databases

PaxDbiO88792.
PeptideAtlasiO88792.
PRIDEiO88792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043839; ENSMUSP00000041907; ENSMUSG00000038235.
GeneIDi16456.
KEGGimmu:16456.
UCSCiuc007doo.1. mouse.

Organism-specific databases

CTDi50848.
MGIiMGI:1321398. F11r.

Phylogenomic databases

eggNOGiENOG410IWUE. Eukaryota.
ENOG410YHHV. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiO88792.
KOiK06089.
OMAiSCSYSGF.
OrthoDBiEOG091G05PI.
TreeFamiTF331459.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-MMU-420029. Tight junction interactions.

Miscellaneous databases

ChiTaRSiF11r. mouse.
EvolutionaryTraceiO88792.
PROiO88792.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038235.
CleanExiMM_F11R.
GenevisibleiO88792. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJAM1_MOUSE
AccessioniPrimary (citable) accession number: O88792
Secondary accession number(s): Q8VC39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.