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Protein

Junctional adhesion molecule A

Gene

F11r

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11447115). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed:11447115). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (PubMed:9660867). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (By similarity). Involved in platelet activation (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • actomyosin structure organization Source: MGI
  • cell adhesion Source: MGI
  • epithelial cell differentiation Source: MGI
  • establishment of endothelial intestinal barrier Source: MGI
  • intestinal absorption Source: UniProtKB
  • negative regulation of GTPase activity Source: MGI
  • positive regulation of blood pressure Source: Ensembl
  • positive regulation of GTPase activity Source: MGI
  • protein localization to plasma membrane Source: MGI
  • regulation of actin cytoskeleton reorganization Source: MGI
  • regulation of cytokine production Source: CACAO
  • regulation of membrane permeability Source: UniProtKB
  • response to radiation Source: Ensembl

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-216083 Integrin cell surface interactions
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-420029 Tight junction interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule A
Short name:
JAM-A
Alternative name(s):
Junctional adhesion molecule 1
Short name:
JAM-1
CD_antigen: CD321
Gene namesi
Name:F11r
Synonyms:Jam1, Jcam, Jcam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1321398 F11r

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 238ExtracellularSequence analysisAdd BLAST212
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 299CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001506727 – 300Junctional adhesion molecule AAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi49 ↔ 108Combined sources1 Publication
Disulfide bondi152 ↔ 212Combined sources1 Publication
Glycosylationi185N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei282PhosphoserineBy similarity1
Modified residuei285PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88792
PeptideAtlasiO88792
PRIDEiO88792

PTM databases

iPTMnetiO88792
PhosphoSitePlusiO88792

Expressioni

Gene expression databases

BgeeiENSMUSG00000038235
CleanExiMM_F11R
GenevisibleiO88792 MM

Interactioni

Subunit structurei

Interacts with the ninth PDZ domain of MPDZ (By similarity). Interacts with the first PDZ domain of PARD3 (PubMed:11447115). The association between PARD3 and PARD6B probably disrupts this interaction (PubMed:11447115). Interacts with ITGAL (via I-domain) (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Pick1Q620832EBI-7580837,EBI-77550

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200861, 2 interactors
DIPiDIP-41166N
IntActiO88792, 5 interactors
MINTiO88792
STRINGi10090.ENSMUSP00000041907

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Beta strandi35 – 40Combined sources6
Beta strandi45 – 48Combined sources4
Beta strandi50 – 53Combined sources4
Beta strandi55 – 65Combined sources11
Beta strandi68 – 74Combined sources7
Helixi80 – 82Combined sources3
Turni83 – 85Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi92 – 96Combined sources5
Helixi100 – 102Combined sources3
Beta strandi104 – 112Combined sources9
Beta strandi115 – 129Combined sources15
Beta strandi135 – 137Combined sources3
Beta strandi140 – 143Combined sources4
Beta strandi148 – 153Combined sources6
Beta strandi162 – 167Combined sources6
Beta strandi170 – 173Combined sources4
Beta strandi184 – 186Combined sources3
Beta strandi188 – 190Combined sources3
Turni192 – 194Combined sources3
Beta strandi197 – 201Combined sources5
Helixi204 – 206Combined sources3
Beta strandi208 – 215Combined sources8
Beta strandi217 – 219Combined sources3
Beta strandi227 – 234Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F97X-ray2.50A27-238[»]
ProteinModelPortaliO88792
SMRiO88792
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88792

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 122Ig-like V-type 1Add BLAST95
Domaini134 – 230Ig-like V-type 2Add BLAST97

Domaini

The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.By similarity

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWUE Eukaryota
ENOG410YHHV LUCA
GeneTreeiENSGT00730000110678
HOGENOMiHOG000247041
HOVERGENiHBG000518
InParanoidiO88792
KOiK06089
OMAiNRAFSNS
OrthoDBiEOG091G05PI
TreeFamiTF331459

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SM00406 IGv, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTEGKAGRK LLFLFTSMIL GSLVQGKGSV YTAQSDVQVP ENESIKLTCT
60 70 80 90 100
YSGFSSPRVE WKFVQGSTTA LVCYNSQITA PYADRVTFSS SGITFSSVTR
110 120 130 140 150
KDNGEYTCMV SEEGGQNYGE VSIHLTVLVP PSKPTISVPS SVTIGNRAVL
160 170 180 190 200
TCSEHDGSPP SEYSWFKDGI SMLTADAKKT RAFMNSSFTI DPKSGDLIFD
210 220 230 240 250
PVTAFDSGEY YCQAQNGYGT AMRSEAAHMD AVELNVGGIV AAVLVTLILL
260 270 280 290 300
GLLIFGVWFA YSRGYFERTK KGTAPGKKVI YSQPSTRSEG EFKQTSSFLV
Length:300
Mass (Da):32,424
Last modified:October 3, 2012 - v2
Checksum:i3CE561E8FF3B97EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268R → T in AAC32982 (PubMed:9660867).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89915 mRNA Translation: AAC32982.1
AK033574 mRNA Translation: BAC28369.1
CT010347 mRNA Translation: CAJ18555.1
AC087229 Genomic DNA No translation available.
BC021876 mRNA Translation: AAH21876.1
CCDSiCCDS15496.1
RefSeqiNP_766235.1, NM_172647.2
UniGeneiMm.294882

Genome annotation databases

EnsembliENSMUST00000043839; ENSMUSP00000041907; ENSMUSG00000038235
GeneIDi16456
KEGGimmu:16456
UCSCiuc007doo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiJAM1_MOUSE
AccessioniPrimary (citable) accession number: O88792
Secondary accession number(s): Q8VC39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health