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Protein

Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

Alg10b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (PubMed:9722534, PubMed:14525949). Has a role in maintenance of cochlear outer hair cell function (By similarity).By similarity2 Publications

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.By similarity

GO - Molecular functioni

  • dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity Source: InterPro
  • potassium channel regulator activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT59. Glycosyltransferase Family 59.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseBy similarity (EC:2.4.1.256By similarity)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10-B
Asparagine-linked glycosylation protein 10 homolog B
Potassium channel regulator 1
Gene namesi
Name:Alg10b
Synonyms:Kcr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi708500. Alg10b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 64ExtracellularSequence analysisAdd BLAST37
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 97CytoplasmicSequence analysisAdd BLAST12
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 126ExtracellularSequence analysis8
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 150CytoplasmicSequence analysis3
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 175ExtracellularSequence analysis4
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 256CytoplasmicSequence analysisAdd BLAST60
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283ExtracellularSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 317CytoplasmicSequence analysisAdd BLAST13
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 365ExtracellularSequence analysisAdd BLAST27
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 392CytoplasmicSequence analysis6
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 436ExtracellularSequence analysisAdd BLAST23
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 473CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154491 – 474Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST474

Proteomic databases

PaxDbiO88788.
PRIDEiO88788.

PTM databases

PhosphoSitePlusiO88788.

Expressioni

Tissue specificityi

Highly expressed in brain, skeletal muscle, uterus, small intestine and liver. Moderately expressed in lung and kidney. Weakly expressed in heart and stomach.1 Publication

Gene expression databases

BgeeiENSRNOG00000014511.

Interactioni

Subunit structurei

Interacts with KCNH1 and KCNH2.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019672.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2642. Eukaryota.
ENOG410YGXE. LUCA.
GeneTreeiENSGT00390000012906.
HOGENOMiHOG000240902.
HOVERGENiHBG053222.
InParanoidiO88788.
KOiK03850.
OMAiHTRKGFA.
OrthoDBiEOG091G0A27.
PhylomeDBiO88788.
TreeFamiTF300150.

Family and domain databases

InterProiIPR016900. Alg10.
[Graphical view]
PANTHERiPTHR12989. PTHR12989. 1 hit.
PfamiPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.

Sequencei

Sequence statusi: Complete.

O88788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GRFSLSQWDP MITTLPGLYL VSVGVVKPAS WILGWSEHVV CSIGMLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFRKIQP RNKASSSIQR ILSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSVFFT LFAYLMCLYG NHRTSALLGF CGFMFRQTNI IWAAFCAGHI
210 220 230 240 250
IAQKCSEAWK TELQKKKEER LPPAKGPLSE LRRVLQFLLM YSMSLKNLSM
260 270 280 290 300
LFLLTWPYML LLLAFFVFVV VNGGIVVGDR SSHEACLHFP QLFYFFSFTA
310 320 330 340 350
FFSFPHLLSP TKVKTFLSLV WKRRVQFSVI TLVSVFLVWK FTYVHKYLLA
360 370 380 390 400
DNRHYTFYVW KRVFQRHEIV KYLLVPAYMF AGWAVADSLK SKSIFWNLMF
410 420 430 440 450
FVCLVASTVP QKLLEFRYFI LPYIIYRLNM PLPPISRLVC ELGCYAVVNF
460 470
LTFYIFLNKT FQWSDSHDIQ RFMW
Length:474
Mass (Da):55,574
Last modified:November 1, 1998 - v1
Checksum:i0901C1DEA9C7B9E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78090 mRNA. Translation: AAC34249.1.
RefSeqiNP_620801.1. NM_139101.2.
UniGeneiRn.234407.

Genome annotation databases

EnsembliENSRNOT00000019672; ENSRNOP00000019672; ENSRNOG00000014511.
GeneIDi245960.
KEGGirno:245960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78090 mRNA. Translation: AAC34249.1.
RefSeqiNP_620801.1. NM_139101.2.
UniGeneiRn.234407.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019672.

Protein family/group databases

CAZyiGT59. Glycosyltransferase Family 59.

PTM databases

PhosphoSitePlusiO88788.

Proteomic databases

PaxDbiO88788.
PRIDEiO88788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019672; ENSRNOP00000019672; ENSRNOG00000014511.
GeneIDi245960.
KEGGirno:245960.

Organism-specific databases

CTDi84920.
RGDi708500. Alg10b.

Phylogenomic databases

eggNOGiKOG2642. Eukaryota.
ENOG410YGXE. LUCA.
GeneTreeiENSGT00390000012906.
HOGENOMiHOG000240902.
HOVERGENiHBG053222.
InParanoidiO88788.
KOiK03850.
OMAiHTRKGFA.
OrthoDBiEOG091G0A27.
PhylomeDBiO88788.
TreeFamiTF300150.

Miscellaneous databases

PROiO88788.

Gene expression databases

BgeeiENSRNOG00000014511.

Family and domain databases

InterProiIPR016900. Alg10.
[Graphical view]
PANTHERiPTHR12989. PTHR12989. 1 hit.
PfamiPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAG10B_RAT
AccessioniPrimary (citable) accession number: O88788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.