Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Coagulation factor V

Gene

F5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138CalciumBy similarity1
Metal bindingi139CalciumBy similarity1
Metal bindingi1802CopperBy similarity1
Metal bindingi1804CopperBy similarity1

GO - Molecular functioni

GO - Biological processi

  • blood circulation Source: MGI
  • blood coagulation Source: MGI

Keywordsi

Biological processBlood coagulation, Hemostasis
LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-140875 Common Pathway of Fibrin Clot Formation
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-5694530 Cargo concentration in the ER
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulation factor V
Alternative name(s):
Activated protein C cofactor
Cleaved into the following 2 chains:
Gene namesi
Name:F5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88382 F5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi333R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication1
Mutagenesisi532R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000035871820 – 2183Coagulation factor VAdd BLAST2164
ChainiPRO_000035871920 – 736Coagulation factor V heavy chainBy similarityAdd BLAST717
PropeptideiPRO_0000358720737 – 1533Activation peptide (connecting region)By similarityAdd BLAST797
ChainiPRO_00003587211534 – 2183Coagulation factor V light chainBy similarityAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi381N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei638PhosphothreonineBy similarity1
Modified residuei692SulfotyrosineSequence analysis1
Modified residuei725SulfotyrosineSequence analysis1
Glycosylationi841N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei903PhosphoserineCombined sources1
Glycosylationi959N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi972N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1438N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1811N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1866 ↔ 2020PROSITE-ProRule annotation
Disulfide bondi2025 ↔ 2180PROSITE-ProRule annotation

Post-translational modificationi

Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).By similarity
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.By similarity
Activated protein C inactivates factor V and factor Va by proteolytic degradation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei333 – 334Cleavage; by activated protein CBy similarity2
Sitei532 – 533Cleavage; by activated protein CBy similarity2
Sitei706 – 707Cleavage; by activated protein CBy similarity2
Sitei736 – 737Cleavage; by thrombinBy similarity2
Sitei1004 – 1005Cleavage; by activated protein CBy similarity2
Sitei1029 – 1030Cleavage; by thrombinBy similarity2
Sitei1533 – 1534Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation, Zymogen

Proteomic databases

MaxQBiO88783
PaxDbiO88783
PeptideAtlasiO88783
PRIDEiO88783

PTM databases

iPTMnetiO88783
PhosphoSitePlusiO88783

Expressioni

Gene expression databases

BgeeiENSMUSG00000026579
GenevisibleiO88783 MM

Interactioni

Subunit structurei

Factor Va, the activated form of factor V, is composed of a heavy chain and a light chain, non-covalently bound. The interaction between the two chains is calcium-dependent. Forms heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

IntActiO88783, 2 interactors
MINTiO88783
STRINGi10090.ENSMUSP00000083204

Structurei

3D structure databases

ProteinModelPortaliO88783
SMRiO88783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 328F5/8 type A 1Add BLAST299
Domaini30 – 192Plastocyanin-like 1Add BLAST163
Domaini202 – 328Plastocyanin-like 2Add BLAST127
Domaini347 – 682F5/8 type A 2Add BLAST336
Domaini347 – 524Plastocyanin-like 3Add BLAST178
Domaini534 – 682Plastocyanin-like 4Add BLAST149
Repeati892 – 9111-1Add BLAST20
Repeati1175 – 11832-19
Repeati1184 – 11922-29
Repeati1193 – 12012-39
Repeati1202 – 12102-49
Repeati1211 – 12192-59
Repeati1220 – 12282-69
Repeati1229 – 12372-79
Repeati1238 – 12462-89
Repeati1247 – 12552-99
Repeati1256 – 12642-109
Repeati1265 – 12732-119
Repeati1274 – 12822-129
Repeati1283 – 12912-139
Repeati1292 – 12992-148
Repeati1300 – 13082-159
Repeati1309 – 13162-168
Repeati1317 – 13252-179
Repeati1326 – 13342-189
Repeati1335 – 13412-197
Repeati1342 – 13502-209
Repeati1351 – 13592-219
Repeati1360 – 13682-229
Repeati1369 – 13772-239
Repeati1378 – 13862-249
Repeati1387 – 13952-259
Repeati1396 – 14042-269
Repeati1405 – 14132-279
Repeati1414 – 14222-289
Repeati1423 – 14312-299
Repeati1432 – 14402-309
Repeati1441 – 14492-319
Repeati1452 – 14612-3210
Domaini1538 – 1866F5/8 type A 3Add BLAST329
Domaini1538 – 1711Plastocyanin-like 5Add BLAST174
Domaini1721 – 1866Plastocyanin-like 6Add BLAST146
Domaini1866 – 2020F5/8 type C 1PROSITE-ProRule annotationAdd BLAST155
Domaini2025 – 2180F5/8 type C 2PROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni691 – 1533BBy similarityAdd BLAST843
Regioni892 – 9081 X 17 AA tandem repeatsAdd BLAST17
Regioni1175 – 146132 X 9 AA approximate tandem repeats of [TNP]-L-S-P-D-L-S-Q-TAdd BLAST287

Domaini

Domain B contains 32 X 9 AA tandem repeats, and 1 X 17 AA repeats.

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IJ6Y Eukaryota
ENOG4111F6G LUCA
GeneTreeiENSGT00910000143988
HOGENOMiHOG000082542
HOVERGENiHBG005631
InParanoidiO88783
KOiK03902
OMAiPDLSHTT
OrthoDBiEOG091G00QL
PhylomeDBiO88783
TreeFamiTF329807

Family and domain databases

Gene3Di2.60.120.260, 2 hits
2.60.40.420, 5 hits
InterProiView protein in InterPro
IPR011707 Cu-oxidase_3
IPR033138 Cu_oxidase_CS
IPR008972 Cupredoxin
IPR000421 FA58C
IPR024715 Factor_5/8_like
IPR008979 Galactose-bd-like_sf
PfamiView protein in Pfam
PF07732 Cu-oxidase_3, 3 hits
PF00754 F5_F8_type_C, 2 hits
PIRSFiPIRSF000354 Factors_V_VIII, 1 hit
SMARTiView protein in SMART
SM00231 FA58C, 2 hits
SUPFAMiSSF49503 SSF49503, 6 hits
SSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits
PS00079 MULTICOPPER_OXIDASE1, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLVCPCFFL LVVLGTRWAG WGSHQAEAAQ LRQFYVAAQG ILWNYHPEPT
60 70 80 90 100
DPSLNSIPSF KKIVYREYEQ YFKKEKPRSS NSGLLGPTLY AEVGDVIKVH
110 120 130 140 150
FRNKADKPLS IHPQGIKYSK FSEGASYADH TFPAERKDDA VAPGEEYTYE
160 170 180 190 200
WIVSEDSGPT PDDPPCLTHI YYSYENLTQD FNSGLIGPLL ICKKGTLTED
210 220 230 240 250
GTQKMFDKQH VLLFAVFDES KSRSQSPSLM YTINGFVNKT MPDITVCAHD
260 270 280 290 300
HVSWHLIGMS SGPELFSIHF NGQVLEQNQH KVSTVTLVSA TSTTANMTMS
310 320 330 340 350
PEGRWIVSSL IPKHYQAGMQ AYIDIKNCPK KTRSPKTLTR EQRRYMKRWE
360 370 380 390 400
YFIAAEEVIW NYAPVIPANM DKIYRSQHLD NFSNQIGKHY KKVIYRQYEE
410 420 430 440 450
ETFTKRTDNP SIKQSGILGP VIRAQVRDTL KIVFKNMASR PYSIYPHGVT
460 470 480 490 500
FSPYEDGINS SSTSGSHTTI RPVQPGETFT YKWNILEFDE PTENDAQCLT
510 520 530 540 550
RPYYSDVDVT RDIASGLIGL LLICKSRSLD QRGVQRVADI EQQAVFAVFD
560 570 580 590 600
ENKSWYIEDN INKFCENPDE VKRDDPKFYE SNIMSTINGY VPESISTLGF
610 620 630 640 650
CFDDTVQWHF CSVGTHDDIL TIHFTGHSFI YGRRHEDTLT LFPMRGESVT
660 670 680 690 700
VTMDNVGTWM LTTMNSNPKR RNLRLRFRDV KCNRDYDNED SYEIYEPPAP
710 720 730 740 750
TSMTTRRIHD SLENEFGIDN EDDDYQYLLA SSLGIRSFKN SSLNPEENEF
760 770 780 790 800
NLTALALENS SEFISPSTDR VVDSNSSRIL SKIINNNLKD FQRTLPGSGA
810 820 830 840 850
TVAGTLLRNL IGLDENFVLN SSTEHRSSSY HENDMENPQS NITMVYLLPL
860 870 880 890 900
GPKGSGNREQ DKPKTIKTGR PHMMKHRFSW MKAPAGKTGR HSNPKNSYSG
910 920 930 940 950
MKSEEDIPSE LIPLKQKITS KFLNRRWRVA SEKGSYEIIA ANGEDTDVDK
960 970 980 990 1000
LTNSPQNQNI TVPRGESTSH TNTTRKPSDL PTFSGVGHKS PHVRQEEENS
1010 1020 1030 1040 1050
GFQKRQLFIR TRKKKKNKKL ALHSPLSPRG FDPLRGHNHS PFPDRRLLNH
1060 1070 1080 1090 1100
SLLLHKSNET ALSPDLNQTS PSMSTDRSLP DYNQYSKNDT EQMSSSLDLY
1110 1120 1130 1140 1150
QSVPAEEHSP TFPAQDPDQT HSTTDPSYRS SPPELSQGLD YDLSHDFYPD
1160 1170 1180 1190 1200
DIGLTSFFPD QSQKSSFSSD DDQAIPSSDL SLFTISPELD QTIIYPDLDQ
1210 1220 1230 1240 1250
LLLSPEDNQK TSSPDLGQVP LSPDDNQKTS SPDLGQVSLS PDDNQKTSSP
1260 1270 1280 1290 1300
DLGQVPLSLD DNQKTTSPDL GQVPLSPDDN QMITSPDLGQ VPLSSDNQKT
1310 1320 1330 1340 1350
SSPDLGQVPL FPEDNQNYFL DLSQVPLSSD QNQETSSTDL LTLSPDFGQT
1360 1370 1380 1390 1400
VLSPDLDQLP LPSDNSQVTV SPDLSLLTLS PDFNEIILAP DLGQVTLSPD
1410 1420 1430 1440 1450
LIQTNPALNH GHKASSADPD QASYPPDSGQ ASSLPELNRT LPHPDLTHIP
1460 1470 1480 1490 1500
PPSPSPTLNN TSLSRKFNPL VVVGLSRVDG DDVEIVPSEE PERIDEDYAE
1510 1520 1530 1540 1550
DDFVTYNDPY RTDTRTDVNS SRNPDTIAAW YLRGHGGHKK FYYIAAEEIT
1560 1570 1580 1590 1600
WNYAEFAQSE MDHEDTGHTP KDTTYKKVVF RKYLDSTFTS RDPRAEYEEH
1610 1620 1630 1640 1650
LGILGPVIRA EVDDVIQVRF KNLASRPYSL HAHGLSYEKS SEGKTYEDES
1660 1670 1680 1690 1700
PEWFQEDDAV QPNSSYTYVW HATKRSGPEN PGSACRAWAY YSAVNVERDI
1710 1720 1730 1740 1750
HSGLIGPLLI CRKGTLHMER NLPMDMREFV LLFMVFDEKK SWYYEKSKGS
1760 1770 1780 1790 1800
RRIESPEEKN AHKFYAINGM IYNLPGLRMY EQEWVRLHLL NMGGSRDIHV
1810 1820 1830 1840 1850
VHFHGQTLLD NRTKQHQLGV WPLLPGSFKT LEMKASKPGW WLLDTEVGEN
1860 1870 1880 1890 1900
QVAGMQTPFL IIDKECKMPM GLSTGVISDS QIKASEYLTY WEPRLARLNN
1910 1920 1930 1940 1950
AGSYNAWSIE KTALDFPIKP WIQVDMQKEV VVTGIQTQGA KHYLKSCFTT
1960 1970 1980 1990 2000
EFQVAYSSDQ TNWQIFRGKS GKSVMYFTGN SDGSTIKENR LDPPIVARYI
2010 2020 2030 2040 2050
RIHPTKSYNR PTLRLELQGC EVNGCSTPLG LEDGRIQDKQ ITASSFKKSW
2060 2070 2080 2090 2100
WGDYWEPSLA RLNAQGRVNA WQAKANNNKQ WLQVDLLKIK KVTAIVTQGC
2110 2120 2130 2140 2150
KSLSSEMYVK SYSIQYSDQG VAWKPYRQKS SMVDKIFEGN SNTKGHMKNF
2160 2170 2180
FNPPIISRFI RIIPKTWNQS IALRLELFGC DIY
Length:2,183
Mass (Da):247,230
Last modified:November 1, 1998 - v1
Checksum:iBF0A8AA723F60317
GO

Sequence cautioni

The sequence BAE23393 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52925 mRNA Translation: AAC99553.1
AK137521 mRNA Translation: BAE23393.1 Different initiation.
AK139240 mRNA Translation: BAE23928.1
CCDSiCCDS35754.1
PIRiT42764
RefSeqiNP_032002.1, NM_007976.3
UniGeneiMm.12900

Genome annotation databases

EnsembliENSMUST00000086040; ENSMUSP00000083204; ENSMUSG00000026579
GeneIDi14067
KEGGimmu:14067
UCSCiuc007dic.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFA5_MOUSE
AccessioniPrimary (citable) accession number: O88783
Secondary accession number(s): Q3UTQ2, Q3UV80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health